pycom02g19190

LRR receptor-like serine threonine-protein kinase At3g47570

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
N/A
Physical Location & Seq
Forward (+)
17141119 .. 17141828
710 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 573 bp
ATGGAAGTGTGGACACAAGGAGACGTGTACAGTTTCGGCATCCTACTGTTAGAAATATTTACGGGAAAAAGACCAACCGATAACAGGTTTCAAGGAGCTTCAAACCTTCATAACTTTGTGAAGGCAGCTCTGCCTGATCAGGTGGTAGAGATTGTGGATCTTGTTCTAGTGGACGAAATTATTGATGGGGACAAGAGCGTAAGAAATAGCGGCAATGAGGATAACAAGTTTAAAGAAAGCTTGATTTCAATCTTGGAAGTTGGTGTCGCTTGCTCTGCGGAACTGCCTAGGGAGCGAAAGAACATTAGTCATGCTGTGGCTGAAATGTGTCTGATTAGAAACAAACTTCGAGCAAATTATTTCTTTATATCCAGAATTTACAGGTCTGTTGAAGTTAAATTAGTATTGTATAGTTATATTCGGAATAAATTCGGGGTCGACTTACAATTAGGGAGATATTCCTTCTATCTTGTGTCTGGTACTGCATGGTTCCATTTTGCAAGGACTCTGAATCATTCTTTTGAAGCCCATCCTCTTCATCATAAAAAATCCACTTGCCCAGAAACAACCTGA

Protein Analysis

191

Amino Acids

21.74

Weight (kDa)

7.76

Isoelectric Point (pI)

30.94

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 438
AciI CCGC 2 cut(s) 210, 278
AclWI GGATC 1 cut(s) 165
AcsI RAATTY 2 cut(s) 375, 428
AfaI GTAC 2 cut(s) 29, 481
AfiI CCNNNNNNNGG 1 cut(s) 84
AflIII ACRYGT 1 cut(s) 24
AgsI TTSAA 5 cut(s) 92, 102, 249, 392, 524
AjiI CACGTC 1 cut(s) 25
AluBI AGCT 3 cut(s) 98, 128, 240
AluI AGCT 3 cut(s) 98, 128, 240
Alw26I GTCTC 1 cut(s) 15
AlwI GGATC 1 cut(s) 165
ApeKI GCWGC 1 cut(s) 125
ApoI RAATTY 2 cut(s) 375, 428
Asp700I GAANNNNTTC 1 cut(s) 428
AspA2I CCTAGG 1 cut(s) 287
AvrII CCTAGG 1 cut(s) 287
BbvI GCAGC 1 cut(s) 137
BccI CCATC 2 cut(s) 179, 537
BclI TGATCA 1 cut(s) 136
BcoDI GTCTC 1 cut(s) 15
BfaI CTAG 2 cut(s) 167, 288
BisI GCNGC 2 cut(s) 126, 211
BlnI CCTAGG 1 cut(s) 287
BlsI GCNGC 2 cut(s) 127, 212
BmgBI CACGTC 1 cut(s) 25
BmiI GGNNCC 1 cut(s) 491
BmsI GCATC 1 cut(s) 48
BsaBI GATNNNNATC 1 cut(s) 248
BsaJI CCNNGG 1 cut(s) 287
Bsc4I CCNNNNNNNGG 1 cut(s) 84
Bse3DI GCAATG 1 cut(s) 220
Bse8I GATNNNNATC 1 cut(s) 248
BseDI CCNNGG 1 cut(s) 287
BseGI GGATG 2 cut(s) 39, 529
BseJI GATNNNNATC 1 cut(s) 248
BseLI CCNNNNNNNGG 1 cut(s) 84
BseMI GCAATG 1 cut(s) 220
BseXI GCAGC 1 cut(s) 137
BslFI GGGAC 1 cut(s) 203
BslI CCNNNNNNNGG 1 cut(s) 84
BsmAI GTCTC 1 cut(s) 15
BsmBI CGTCTC 1 cut(s) 15
BsmFI GGGAC 1 cut(s) 203
Bsp1407I TGTACA 1 cut(s) 27
Bsp143I GATC 2 cut(s) 136, 157
BspACI CCGC 2 cut(s) 210, 278
BspLI GGNNCC 1 cut(s) 491
BspPI GGATC 1 cut(s) 165
BsrDI GCAATG 1 cut(s) 220
BsrGI TGTACA 1 cut(s) 27
BssECI CCNNGG 1 cut(s) 287
BssMI GATC 2 cut(s) 136, 157
BssT1I CCWWGG 1 cut(s) 287
Bst4CI ACNGT 2 cut(s) 32, 48
Bst6I CTCTTC 1 cut(s) 540
BstAUI TGTACA 1 cut(s) 27
BstC8I GCNNGC 1 cut(s) 271
BstF5I GGATG 2 cut(s) 39, 529
BstKTI GATC 2 cut(s) 139, 160
BstMAI GTCTC 1 cut(s) 15
BstMBI GATC 2 cut(s) 136, 157
BstMWI GCNNNNNNNGC 2 cut(s) 275, 292
BstV1I GCAGC 1 cut(s) 137
BstX2I RGATCY 1 cut(s) 157
BstYI RGATCY 1 cut(s) 157
BtrI CACGTC 1 cut(s) 25
BtsCI GGATG 2 cut(s) 39, 529
Cac8I GCNNGC 1 cut(s) 271
Csp6I GTAC 2 cut(s) 28, 480
CviAII CATG 2 cut(s) 311, 486
CviJI RGCY 5 cut(s) 98, 128, 240, 320, 527
CviKI_1 RGCY 5 cut(s) 98, 128, 240, 320, 527
CviQI GTAC 2 cut(s) 28, 480
DpnI GATC 2 cut(s) 138, 159
DpnII GATC 2 cut(s) 136, 157
DraI TTTAAA 1 cut(s) 232
Eam1104I CTCTTC 1 cut(s) 540
EarI CTCTTC 1 cut(s) 540
Eco130I CCWWGG 1 cut(s) 287
EcoT14I CCWWGG 1 cut(s) 287
ErhI CCWWGG 1 cut(s) 287
Esp3I CGTCTC 1 cut(s) 15
FaeI CATG 2 cut(s) 314, 489
FaiI YATR 7 cut(s) 111, 312, 368, 411, 417, 487, 543
FaqI GGGAC 1 cut(s) 203
FatI CATG 2 cut(s) 310, 485
FbaI TGATCA 1 cut(s) 136
FblI GTMKAC 1 cut(s) 438
Fnu4HI GCNGC 2 cut(s) 126, 211
FokI GGATG 2 cut(s) 26, 516
Fsp4HI GCNGC 2 cut(s) 126, 211
FspBI CTAG 2 cut(s) 167, 288
GluI GCNGC 2 cut(s) 126, 211
Hin1II CATG 2 cut(s) 314, 489
HincII GTYRAC 1 cut(s) 439
HindII GTYRAC 1 cut(s) 439
HindIII AAGCTT 1 cut(s) 238
HinfI GANTC 2 cut(s) 505, 511
Hpy166II GTNNAC 4 cut(s) 12, 28, 172, 439
Hpy188I TCNGA 3 cut(s) 333, 423, 510
Hpy188III TCNNGA 1 cut(s) 372
Hpy8I GTNNAC 4 cut(s) 12, 28, 172, 439
HpyAV CCTTC 3 cut(s) 115, 116, 472
HpyCH4III ACNGT 2 cut(s) 32, 48
HpyCH4IV ACGT 1 cut(s) 24
HpyCH4V TGCA 2 cut(s) 485, 500
HpyF10VI GCNNNNNNNGC 2 cut(s) 275, 292
HpySE526I ACGT 1 cut(s) 24
Hsp92II CATG 2 cut(s) 314, 489
Ksp22I TGATCA 1 cut(s) 136
Kzo9I GATC 2 cut(s) 136, 157
LmnI GCTCC 2 cut(s) 95, 292
LpnPI CCDG 6 cut(s) 70, 125, 147, 367, 385, 462
Lsp1109I GCAGC 1 cut(s) 137
LweI GCATC 1 cut(s) 48
MaeI CTAG 2 cut(s) 167, 288
MaeII ACGT 1 cut(s) 24
MalI GATC 2 cut(s) 138, 159
MboI GATC 2 cut(s) 136, 157
MboII GAAGA 1 cut(s) 527
MflI RGATCY 1 cut(s) 157
MluCI AATT 6 cut(s) 177, 355, 375, 398, 428, 446
MlyI GAGTC 1 cut(s) 499
MnlI CCTC 2 cut(s) 211, 543
MroXI GAANNNNTTC 1 cut(s) 428
MseI TTAA 2 cut(s) 231, 396
MwoI GCNNNNNNNGC 2 cut(s) 275, 292
NdeII GATC 2 cut(s) 136, 157
NlaIII CATG 2 cut(s) 314, 489
NlaIV GGNNCC 1 cut(s) 491
PdmI GAANNNNTTC 1 cut(s) 428
PfeI GAWTC 1 cut(s) 511
PkrI GCNGC 2 cut(s) 127, 212
PleI GAGTC 1 cut(s) 499
PpsI GAGTC 1 cut(s) 499
PspN4I GGNNCC 1 cut(s) 491
PsuI RGATCY 1 cut(s) 157
RsaI GTAC 2 cut(s) 29, 481
RsaNI GTAC 2 cut(s) 28, 480
SalI GTCGAC 1 cut(s) 437
SaqAI TTAA 2 cut(s) 231, 396
SatI GCNGC 2 cut(s) 126, 211
Sau3AI GATC 2 cut(s) 136, 157
SchI GAGTC 1 cut(s) 499
SetI ASST 9 cut(s) 27, 89, 100, 108, 130, 144, 242, 386, 572
SfaNI GCATC 1 cut(s) 48
Sse9I AATT 6 cut(s) 177, 355, 375, 398, 428, 446
SsiI CCGC 2 cut(s) 210, 278
SspI AATATT 1 cut(s) 57
SspMI CTAG 2 cut(s) 167, 288
StyI CCWWGG 1 cut(s) 287
TaaI ACNGT 2 cut(s) 32, 48
TaiI ACGT 1 cut(s) 27
TaqI TCGA 2 cut(s) 349, 438
TasI AATT 6 cut(s) 177, 355, 375, 398, 428, 446
TatI WGTACW 1 cut(s) 27
TauI GCSGC 1 cut(s) 213
TfiI GAWTC 1 cut(s) 511
Tru1I TTAA 2 cut(s) 231, 396
Tru9I TTAA 2 cut(s) 231, 396
TseI GCWGC 1 cut(s) 125
TspDTI ATGAA 2 cut(s) 98, 527
XapI RAATTY 2 cut(s) 375, 428
XmaJI CCTAGG 1 cut(s) 287
XmiI GTMKAC 1 cut(s) 438
XmnI GAANNNNTTC 1 cut(s) 428
XspI CTAG 2 cut(s) 167, 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.