pycom02g19380

Ras-related protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
N/A
Physical Location & Seq
Forward (+)
17220416 .. 17226169
5754 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1836 bp
ATGCCCTCCTTCGGGACGGGGTGTGTCAATTCGGTCATTTGTGGTCTCTCAGTCGCTCACTCTCACAGTCTCACACTTAATCTCCTAAAGTTGCTCGTGCTCCCGCCGCTCCTCCATCGACTCACGCCGTTCATTCCGTCCTCTGTCCACTTCAACGGCGCTCATTCAGCCAGCCACACACATAGAGATGTAGATGCAGGAAGACGCAAATCCGACACCAATCGAGTCCCAAAATCCAGTTCAACGCCGCCCATCACTACCTCCGTGACCACCTATTTCAAGAGCATCTACACCAACACAAATGAGGGTTCAATTATCAACAAGAGGACGGCGTCCACCATCAATGGCAACGGGTCATTCATCAACAAGGGGAAGGGGTCGAAGGGGCGAAAGGGAATGAACTTACTTAAGCTTCATTTTGGTTGCATGTACTTTTTTGCTTATAAACTATGTTTTTCTTTTGGTTTTTCATTTTGGTTGCATGCACTTTTAATTAACAGTTTGGTAGACAATTGTTGTGGGCAGTGGGCTGCAATGGCCTTACTTGTTAAAGAGCGTAAAATTGTAAAAAGTTTAAAAAGCCCAACATTTTGGGCTTCAAGGCCCAATCTGAAAGTTTCCCAAAAATCTCGAAACACTTTCGGGAATCCCGATTTTCGGGAAATGAATAGTTTCGGTTCTATTCGGGTTAGGGGTTTCTGTTCGAGATCGATCTTCATGACCCAAACCAGCCCTATAAATAATTTCCTTTTCGTGGTAAAACCCCCTCAAACCAATTTCACGCGCAAAATAGTCTCTTTCTGTCTCTCTCTCTCTGCCAGCGGGTCATCGCGCTATAAATACGCATCCAACCGAAAATCGAAGGAAAGAAGCGAATTTCGAACGAGGCTCCGAGATCTCGATCTATCCGAAATCGCCATGGCTCCCGTTTCGGCTCTCGCCAAGTACAAACTCGTCTTCCTGGGGGATCAGTCCGTCGGGAAAACCAGCATCATCACTCGCTTCATGTACGACAAGTTCGACAATACCTATCAGGCTACCATCGGCATTGATTTTCTGTCAAAGACAATGTATCTTGAAGATCGAACCGTTCGATTGCAGCTCTGGGATACTGCTGGGCAGGAAAGGTTCAGGAGTCTCATTCCTAGCTACATCAGGGATTCCTCTGTGGCTGTCATTGTATATGATGTTGCAAGCAGACAATCATTCCTAAACACTTCCAAGTGGATTGAAGAGGTTCGCACTGAGCGGGGAAGTGATGTTATCATTGTACTTGTTGGAAACAAAACCGATCTTGTAGACAAAAGGCAAGTTTCTATAGAGGAAGGAGAAGCCAAAGCTCGTGACCTCAATGTTATGTTTATCGAGACCAGTGCCAAGGCTGGCTTCAATATCAAGGCGCTGTTTCGGAAGATTGCAGCAGCTTTACCTGGAATGGAAACACTTTCTTCAACGAAGCAGGAGGACATGGTTGACGTGAACCTGAAGTCTTCGAATGCAAATTCATCACAGTCACAGGCACAGTCAGGCGGGTGCGCTTCTGTAAAGATTAGTTACTGTCCCAGTCGTCCCGCCATTTTGTACAGACTTTCAGATTTTCAGTTAATAGTAATTAAAAGAAACATTTACATTTTTTTTCTGCAAACTAATTTTAATAAATTCCTATGGTTGTATTCTACGGTGTGTATTGTACAATTATTAATACTAGTCTATTGGCATGCGCTTGAACTGATTACCTTTCTTTGTTATTTATGGGTTTATAATTTGTTTTGTAACGAATATAGAGAAATAAAAACAAAAGTTAAAATAGATATAATTTATAATTTATTCTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ras

Protein Analysis

612

Amino Acids

69.09

Weight (kDa)

9.78

Isoelectric Point (pI)

34.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Arf PF00025 314 - 472 4.6e-14 ADP-ribosylation factor family
Ras PF00071 317 - 475 1.6e-57 Ras family
Roc PF08477 317 - 431 1.3e-36 Ras of Complex, Roc, domain of DAPkinase
GTP_EFTU PF00009 349 - 477 7.6e-06 Elongation factor Tu GTP binding domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 444, 1761, 1821
AccBSI CCGCTC 2 cut(s) 109, 1249
AccI GTMKAC 2 cut(s) 507, 1299
AccII CGCG 2 cut(s) 784, 832
AciI CCGC 7 cut(s) 104, 107, 248, 822, 1249, 1530, 1572
AclWI GGATC 1 cut(s) 975
AcsI RAATTY 3 cut(s) 875, 1501, 1658
AcuI CTGAAG 1 cut(s) 1505
AcyI GRCGYC 1 cut(s) 332
AfaI GTAC 6 cut(s) 431, 947, 1010, 1272, 1583, 1692
AfiI CCNNNNNNNGG 4 cut(s) 11, 12, 657, 754
AflII CTTAAG 1 cut(s) 407
AhlI ACTAGT 1 cut(s) 1705
AjiI CACGTC 1 cut(s) 1477
AjnI CCWGG 2 cut(s) 960, 1429
AjuI GAANNNNNNNTTGG 4 cut(s) 223, 255, 1370, 1402
AluBI AGCT 5 cut(s) 412, 1102, 1149, 1340, 1424
AluI AGCT 5 cut(s) 412, 1102, 1149, 1340, 1424
Alw21I GWGCWC 1 cut(s) 102
Alw26I GTCTC 6 cut(s) 50, 74, 799, 809, 1142, 1361
AlwI GGATC 1 cut(s) 975
AoxI GGCC 2 cut(s) 537, 602
ApeKI GCWGC 4 cut(s) 530, 1099, 1418, 1421
ApoI RAATTY 3 cut(s) 875, 1501, 1658
AseI ATTAAT 1 cut(s) 1700
Asp700I GAANNNNTTC 2 cut(s) 671, 1236
AspLEI GCGC 6 cut(s) 161, 786, 834, 1402, 1538, 1723
AspS9I GGNCC 1 cut(s) 603
AsuII TTCGAA 2 cut(s) 880, 1493
BauI CACGAG 2 cut(s) 95, 1341
BbsI GAAGAC 3 cut(s) 208, 949, 1482
Bbv12I GWGCWC 1 cut(s) 102
BbvI GCAGC 4 cut(s) 517, 1111, 1430, 1433
BccI CCATC 4 cut(s) 123, 260, 347, 1049
BceAI ACGGC 3 cut(s) 112, 172, 345
BcgI CGANNNNNNTGC 2 cut(s) 1355, 1389
BciT130I CCWGG 2 cut(s) 962, 1431
BciVI GTATCC 1 cut(s) 1102
BcoDI GTCTC 6 cut(s) 50, 74, 799, 809, 1142, 1361
BcuI ACTAGT 1 cut(s) 1705
BfaI CTAG 2 cut(s) 1146, 1706
BfmI CTRYAG 1 cut(s) 1317
BfoI RGCGCY 2 cut(s) 162, 1403
BfrI CTTAAG 1 cut(s) 407
BfuI GTATCC 1 cut(s) 1102
BglII AGATCT 1 cut(s) 895
BisI GCNGC 6 cut(s) 107, 248, 531, 1100, 1419, 1422
BlsI GCNGC 6 cut(s) 108, 249, 532, 1101, 1420, 1423
Bme1390I CCNGG 2 cut(s) 962, 1431
BmgBI CACGTC 1 cut(s) 1477
BmgT120I GGNCC 1 cut(s) 603
BmiI GGNNCC 2 cut(s) 890, 924
BmrFI CCNGG 2 cut(s) 962, 1431
BmrI ACTGGG 1 cut(s) 1557
BmsI GCATC 4 cut(s) 184, 294, 854, 999
BmuI ACTGGG 1 cut(s) 1557
BpiI GAAGAC 3 cut(s) 208, 949, 1482
Bpu14I TTCGAA 2 cut(s) 880, 1493
Bsa29I ATCGAT 1 cut(s) 710
BsaBI GATNNNNATC 1 cut(s) 900
BsaHI GRCGYC 1 cut(s) 332
BsaI GGTCTC 2 cut(s) 50, 1361
BsaJI CCNNGG 3 cut(s) 918, 961, 1377
BsaXI ACNNNNNCTCC 2 cut(s) 66, 96
Bsc4I CCNNNNNNNGG 4 cut(s) 11, 12, 657, 754
Bse1I ACTGG 3 cut(s) 237, 1371, 1563
Bse3DI GCAATG 1 cut(s) 540
Bse8I GATNNNNATC 1 cut(s) 900
BseBI CCWGG 2 cut(s) 962, 1431
BseCI ATCGAT 1 cut(s) 710
BseDI CCNNGG 3 cut(s) 918, 961, 1377
BseGI GGATG 1 cut(s) 845
BseJI GATNNNNATC 1 cut(s) 900
BseLI CCNNNNNNNGG 4 cut(s) 11, 12, 657, 754
BseMI GCAATG 1 cut(s) 540
BseMII CTCAG 2 cut(s) 63, 1236
BseNI ACTGG 3 cut(s) 237, 1371, 1563
BseRI GAGGAG 1 cut(s) 101
BseXI GCAGC 4 cut(s) 517, 1111, 1430, 1433
BseYI CCCAGC 1 cut(s) 1115
Bsh1236I CGCG 2 cut(s) 784, 832
BshFI GGCC 2 cut(s) 539, 604
BshVI ATCGAT 1 cut(s) 710
BsiHKAI GWGCWC 1 cut(s) 102
BslFI GGGAC 4 cut(s) 28, 212, 1545, 1554
BslI CCNNNNNNNGG 4 cut(s) 11, 12, 657, 754
BsmAI GTCTC 6 cut(s) 50, 74, 799, 809, 1142, 1361
BsmFI GGGAC 4 cut(s) 28, 212, 1545, 1554
BsmI GAATGC 1 cut(s) 1501
BsnI GGCC 2 cut(s) 539, 604
Bso31I GGTCTC 2 cut(s) 50, 1361
Bsp119I TTCGAA 2 cut(s) 880, 1493
Bsp1286I GDGCHC 1 cut(s) 102
Bsp1407I TGTACA 2 cut(s) 1581, 1690
Bsp143I GATC 7 cut(s) 707, 711, 895, 901, 967, 1081, 1291
Bsp19I CCATGG 1 cut(s) 918
BspACI CCGC 7 cut(s) 104, 107, 248, 822, 1249, 1530, 1572
BspANI GGCC 2 cut(s) 539, 604
BspCNI CTCAG 2 cut(s) 62, 1237
BspDI ATCGAT 1 cut(s) 710
BspFNI CGCG 2 cut(s) 784, 832
BspHI TCATGA 1 cut(s) 717
BspLI GGNNCC 2 cut(s) 890, 924
BspPI GGATC 1 cut(s) 975
BspT104I TTCGAA 2 cut(s) 880, 1493
BspTI CTTAAG 1 cut(s) 407
BspTNI GGTCTC 2 cut(s) 50, 1361
BsrBI CCGCTC 2 cut(s) 109, 1249
BsrDI GCAATG 1 cut(s) 540
BsrGI TGTACA 2 cut(s) 1581, 1690
BsrI ACTGG 3 cut(s) 237, 1371, 1563
BssECI CCNNGG 3 cut(s) 918, 961, 1377
BssMI GATC 7 cut(s) 707, 711, 895, 901, 967, 1081, 1291
BssNI GRCGYC 1 cut(s) 332
BssSI CACGAG 2 cut(s) 95, 1341
BssT1I CCWWGG 2 cut(s) 918, 1377
Bst2BI CACGAG 2 cut(s) 95, 1341
Bst2UI CCWGG 2 cut(s) 962, 1431
Bst4CI ACNGT 7 cut(s) 68, 500, 1090, 1512, 1524, 1559, 1681
Bst6I CTCTTC 1 cut(s) 1227
BstACI GRCGYC 1 cut(s) 332
BstAFI CTTAAG 1 cut(s) 407
BstAUI TGTACA 2 cut(s) 1581, 1690
BstBI TTCGAA 2 cut(s) 880, 1493
BstC8I GCNNGC 6 cut(s) 172, 483, 820, 1195, 1384, 1719
BstDEI CTNAG 2 cut(s) 49, 1245
BstDSI CCRYGG 1 cut(s) 918
BstF5I GGATG 1 cut(s) 845
BstFNI CGCG 2 cut(s) 784, 832
BstH2I RGCGCY 2 cut(s) 162, 1403
BstHHI GCGC 6 cut(s) 161, 786, 834, 1402, 1538, 1723
BstKTI GATC 7 cut(s) 710, 714, 898, 904, 970, 1084, 1294
BstMAI GTCTC 6 cut(s) 50, 74, 799, 809, 1142, 1361
BstMBI GATC 7 cut(s) 707, 711, 895, 901, 967, 1081, 1291
BstMWI GCNNNNNNNGC 3 cut(s) 106, 167, 536
BstNI CCWGG 2 cut(s) 962, 1431
BstNSI RCATGY 3 cut(s) 430, 485, 1721
BstSCI CCNGG 2 cut(s) 960, 1429
BstSFI CTRYAG 1 cut(s) 1317
BstUI CGCG 2 cut(s) 784, 832
BstV1I GCAGC 4 cut(s) 517, 1111, 1430, 1433
BstV2I GAAGAC 3 cut(s) 208, 949, 1482
BstX2I RGATCY 1 cut(s) 895
BstXI CCANNNNNNTGG 1 cut(s) 591
BstYI RGATCY 1 cut(s) 895
Bsu15I ATCGAT 1 cut(s) 710
BsuI GTATCC 1 cut(s) 1102
BsuRI GGCC 2 cut(s) 539, 604
BsuTUI ATCGAT 1 cut(s) 710
BtgI CCRYGG 1 cut(s) 918
BtgZI GCGATG 1 cut(s) 813
BtrI CACGTC 1 cut(s) 1477
BtsCI GGATG 1 cut(s) 845
BtsI GCAGTG 1 cut(s) 530
BtsIMutI CAGTG 3 cut(s) 530, 1242, 1378
Cac8I GCNNGC 6 cut(s) 172, 483, 820, 1195, 1384, 1719
CciI TCATGA 1 cut(s) 717
CfoI GCGC 6 cut(s) 161, 786, 834, 1402, 1538, 1723
Cfr13I GGNCC 1 cut(s) 603
ClaI ATCGAT 1 cut(s) 710
CseI GACGC 2 cut(s) 213, 321
Csp6I GTAC 6 cut(s) 430, 946, 1009, 1271, 1582, 1691
CspCI CAANNNNNGTGG 2 cut(s) 499, 534
CviAII CATG 7 cut(s) 427, 482, 718, 919, 1006, 1468, 1718
CviQI GTAC 6 cut(s) 430, 946, 1009, 1271, 1582, 1691
DdeI CTNAG 2 cut(s) 49, 1245
DpnI GATC 7 cut(s) 709, 713, 897, 903, 969, 1083, 1293
DpnII GATC 7 cut(s) 707, 711, 895, 901, 967, 1081, 1291
DraI TTTAAA 1 cut(s) 576
Eam1104I CTCTTC 1 cut(s) 1227
EarI CTCTTC 1 cut(s) 1227
Eco130I CCWWGG 2 cut(s) 918, 1377
Eco31I GGTCTC 2 cut(s) 50, 1361
Eco57I CTGAAG 1 cut(s) 1505
EcoRII CCWGG 2 cut(s) 960, 1429
EcoT14I CCWWGG 2 cut(s) 918, 1377
ErhI CCWWGG 2 cut(s) 918, 1377
FaeI CATG 7 cut(s) 430, 485, 721, 922, 1009, 1471, 1721
FalI AAGNNNNNCTT 2 cut(s) 1370, 1402
FaqI GGGAC 4 cut(s) 28, 212, 1545, 1554
FatI CATG 7 cut(s) 426, 481, 717, 918, 1005, 1467, 1717
FauI CCCGC 5 cut(s) 111, 815, 1242, 1523, 1579
FblI GTMKAC 2 cut(s) 507, 1299
Fnu4HI GCNGC 6 cut(s) 107, 248, 531, 1100, 1419, 1422
FokI GGATG 1 cut(s) 832
Fsp4HI GCNGC 6 cut(s) 107, 248, 531, 1100, 1419, 1422
FspBI CTAG 2 cut(s) 1146, 1706
GlaI GCGC 6 cut(s) 160, 785, 833, 1401, 1537, 1722
GluI GCNGC 6 cut(s) 107, 248, 531, 1100, 1419, 1422
GsaI CCCAGC 1 cut(s) 1119
HaeII RGCGCY 2 cut(s) 162, 1403
HaeIII GGCC 2 cut(s) 539, 604
HgaI GACGC 2 cut(s) 213, 321
HhaI GCGC 6 cut(s) 161, 786, 834, 1402, 1538, 1723
Hin1I GRCGYC 1 cut(s) 332
Hin1II CATG 7 cut(s) 430, 485, 721, 922, 1009, 1471, 1721
Hin6I GCGC 6 cut(s) 159, 784, 832, 1400, 1536, 1721
HinP1I GCGC 6 cut(s) 159, 784, 832, 1400, 1536, 1721
HincII GTYRAC 1 cut(s) 1474
HindII GTYRAC 1 cut(s) 1474
HindIII AAGCTT 1 cut(s) 410
HinfI GANTC 5 cut(s) 120, 225, 646, 1135, 1160
Hpy166II GTNNAC 6 cut(s) 148, 336, 508, 1300, 1474, 1480
Hpy188I TCNGA 6 cut(s) 214, 612, 893, 910, 1410, 1594
Hpy8I GTNNAC 6 cut(s) 148, 336, 508, 1300, 1474, 1480
Hpy99I CGWCG 1 cut(s) 980
HpyAV CCTTC 5 cut(s) 19, 367, 376, 856, 1319
HpyCH4III ACNGT 7 cut(s) 68, 500, 1090, 1512, 1524, 1559, 1681
HpyCH4IV ACGT 1 cut(s) 1476
HpyF10VI GCNNNNNNNGC 3 cut(s) 106, 167, 536
HpyF3I CTNAG 2 cut(s) 49, 1245
HpySE526I ACGT 1 cut(s) 1476
Hsp92I GRCGYC 1 cut(s) 332
Hsp92II CATG 7 cut(s) 430, 485, 721, 922, 1009, 1471, 1721
HspAI GCGC 6 cut(s) 159, 784, 832, 1400, 1536, 1721
Kzo9I GATC 7 cut(s) 707, 711, 895, 901, 967, 1081, 1291
LmnI GCTCC 4 cut(s) 105, 114, 894, 928
Lsp1109I GCAGC 4 cut(s) 517, 1111, 1430, 1433
LweI GCATC 4 cut(s) 184, 294, 854, 999
MaeI CTAG 2 cut(s) 1146, 1706
MaeII ACGT 1 cut(s) 1476
MaeIII GTNAC 5 cut(s) 265, 1343, 1512, 1553, 1772
MalI GATC 7 cut(s) 709, 713, 897, 903, 969, 1083, 1293
MbiI CCGCTC 2 cut(s) 109, 1249
MboI GATC 7 cut(s) 707, 711, 895, 901, 967, 1081, 1291
MboII GAAGA 8 cut(s) 213, 706, 949, 1091, 1244, 1423, 1440, 1482
MfeI CAATTG 1 cut(s) 511
MflI RGATCY 1 cut(s) 895
MhlI GDGCHC 1 cut(s) 102
MlyI GAGTC 3 cut(s) 114, 234, 1144
MmeI TCCRAC 3 cut(s) 237, 873, 1258
MroXI GAANNNNTTC 2 cut(s) 671, 1236
MslI CAYNNNNRTG 1 cut(s) 186
MspA1I CMGCKG 1 cut(s) 822
MspCI CTTAAG 1 cut(s) 407
MspR9I CCNGG 2 cut(s) 962, 1431
MunI CAATTG 1 cut(s) 511
Mva1269I GAATGC 1 cut(s) 1501
MvaI CCWGG 2 cut(s) 962, 1431
MvnI CGCG 2 cut(s) 784, 832
MwoI GCNNNNNNNGC 3 cut(s) 106, 167, 536
NcoI CCATGG 1 cut(s) 918
NdeII GATC 7 cut(s) 707, 711, 895, 901, 967, 1081, 1291
NlaIII CATG 7 cut(s) 430, 485, 721, 922, 1009, 1471, 1721
NlaIV GGNNCC 2 cut(s) 890, 924
NmuCI GTSAC 3 cut(s) 265, 1343, 1512
NspI RCATGY 3 cut(s) 430, 485, 1721
NspV TTCGAA 2 cut(s) 880, 1493
PacI TTAATTAA 1 cut(s) 495
PaeI GCATGC 2 cut(s) 485, 1721
PagI TCATGA 1 cut(s) 717
PcsI WCGNNNNNNNCGW 4 cut(s) 648, 906, 1017, 1090
PctI GAATGC 1 cut(s) 1501
PdmI GAANNNNTTC 2 cut(s) 671, 1236
PfeI GAWTC 2 cut(s) 646, 1160
PflFI GACNNNGTC 1 cut(s) 331
PkrI GCNGC 6 cut(s) 108, 249, 532, 1101, 1420, 1423
PleI GAGTC 3 cut(s) 114, 233, 1143
PpsI GAGTC 3 cut(s) 114, 233, 1143
PshBI ATTAAT 1 cut(s) 1700
PsiI TTATAA 3 cut(s) 444, 1761, 1821
Psp6I CCWGG 2 cut(s) 960, 1429
PspFI CCCAGC 1 cut(s) 1115
PspGI CCWGG 2 cut(s) 960, 1429
PspN4I GGNNCC 2 cut(s) 890, 924
PspPI GGNCC 1 cut(s) 603
PsrI GAACNNNNNNTAC 2 cut(s) 1001, 1033
PsuI RGATCY 1 cut(s) 895
PsyI GACNNNGTC 1 cut(s) 331
RsaI GTAC 6 cut(s) 431, 947, 1010, 1272, 1583, 1692
RsaNI GTAC 6 cut(s) 430, 946, 1009, 1271, 1582, 1691
RseI CAYNNNNRTG 1 cut(s) 186
SatI GCNGC 6 cut(s) 107, 248, 531, 1100, 1419, 1422
Sau3AI GATC 7 cut(s) 707, 711, 895, 901, 967, 1081, 1291
Sau96I GGNCC 1 cut(s) 603
SchI GAGTC 3 cut(s) 114, 234, 1144
ScrFI CCNGG 2 cut(s) 962, 1431
SduI GDGCHC 1 cut(s) 102
SfaNI GCATC 4 cut(s) 184, 294, 854, 999
SfcI CTRYAG 1 cut(s) 1317
SfuI TTCGAA 2 cut(s) 880, 1493
SmiMI CAYNNNNRTG 1 cut(s) 186
SmlI CTYRAG 1 cut(s) 407
SmoI CTYRAG 1 cut(s) 407
SpeI ACTAGT 1 cut(s) 1705
SphI GCATGC 2 cut(s) 485, 1721
SsiI CCGC 7 cut(s) 104, 107, 248, 822, 1249, 1530, 1572
SspMI CTAG 2 cut(s) 1146, 1706
StyD4I CCNGG 2 cut(s) 960, 1429
StyI CCWWGG 2 cut(s) 918, 1377
TaaI ACNGT 7 cut(s) 68, 500, 1090, 1512, 1524, 1559, 1681
TaiI ACGT 1 cut(s) 1479
TaqII GACCGA 1 cut(s) 22
TatI WGTACW 5 cut(s) 429, 945, 1270, 1581, 1690
TauI GCSGC 2 cut(s) 109, 250
TfiI GAWTC 2 cut(s) 646, 1160
TscAI CASTG 3 cut(s) 530, 1249, 1378
TseFI GTSAC 3 cut(s) 265, 1343, 1512
TseI GCWGC 4 cut(s) 530, 1099, 1418, 1421
Tsp45I GTSAC 3 cut(s) 265, 1343, 1512
TspDTI ATGAA 9 cut(s) 121, 349, 404, 413, 459, 680, 706, 994, 1494
TspGWI ACGGA 3 cut(s) 126, 253, 964
TspRI CASTG 3 cut(s) 530, 1249, 1378
Tth111I GACNNNGTC 1 cut(s) 331
Vha464I CTTAAG 1 cut(s) 407
VspI ATTAAT 1 cut(s) 1700
XapI RAATTY 3 cut(s) 875, 1501, 1658
XceI RCATGY 3 cut(s) 430, 485, 1721
XmiI GTMKAC 2 cut(s) 507, 1299
XmnI GAANNNNTTC 2 cut(s) 671, 1236
XspI CTAG 2 cut(s) 1146, 1706
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.