pycom02g19590

Rgp1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
N/A
Physical Location & Seq
Forward (+)
17486697 .. 17488926
2230 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 528 bp
ATGATGAAGCAAGAGGGCTTCATTCGAGGGAGGTCATACAATATCCGGCTGGATGACCGAGTTCTGCTTAGATTTTCCCCAAAAAATTCTGATTCCAATTATTACTTCTGTGATATGATTGGTGGAACTCTTACTTTCTTTCATGAAGAAGGAGCTAGAAGATGCCTTGAGGTAGATGCAATTAGAAGTAATATTGAAATCAATTATAGTGTTTCTATAACATTGGAGACTTCAGAAACTATTAGCCAGCGCTTTGTGCATCCTTCTCGGAGGAATTCTCCCACAATTACAAAGGGTACGTGCAATCATGTACCAGAGATCCAGAGTGATCATTATGAGGTTGTAGCGGACTTGGTGCAGACAAGTTTTCTTTTCTCCATTCCCATGGATGGGCCTATGTCTTTTTCCACTCCCCATGTTTCTGTACAATGGGAACTTCGATTTGAATTTTTTACCACTCCGAAGAATGTGGATTGGACCAGGTATGACCGACCATGTGGAATCATTCATGAGCTTCTTATTTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rgp1

Protein Analysis

176

Amino Acids

20.32

Weight (kDa)

5.85

Isoelectric Point (pI)

56.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Rgp1 PF08737 64 - 152 1e-07 Rgp1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 347
AclWI GGATC 1 cut(s) 313
AcsI RAATTY 3 cut(s) 85, 274, 446
AcuI CTGAAG 1 cut(s) 216
AfaI GTAC 3 cut(s) 298, 312, 426
AfeI AGCGCT 1 cut(s) 251
AfiI CCNNNNNNNGG 2 cut(s) 389, 390
AgsI TTSAA 2 cut(s) 197, 446
AjnI CCWGG 1 cut(s) 479
AjuI GAANNNNNNNTTGG 2 cut(s) 89, 121
AluBI AGCT 2 cut(s) 155, 514
AluI AGCT 2 cut(s) 155, 514
Alw26I GTCTC 1 cut(s) 221
AlwI GGATC 1 cut(s) 313
Aor51HI AGCGCT 1 cut(s) 251
AoxI GGCC 1 cut(s) 392
ApoI RAATTY 3 cut(s) 85, 274, 446
AspLEI GCGC 1 cut(s) 252
AspS9I GGNCC 2 cut(s) 392, 477
AvaII GGWCC 1 cut(s) 477
BccI CCATC 1 cut(s) 383
BcgI CGANNNNNNTGC 2 cut(s) 248, 282
BciT130I CCWGG 1 cut(s) 481
BclI TGATCA 1 cut(s) 328
BcoDI GTCTC 1 cut(s) 221
BfaI CTAG 1 cut(s) 156
BfoI RGCGCY 1 cut(s) 253
Bme1390I CCNGG 1 cut(s) 481
Bme18I GGWCC 1 cut(s) 477
BmgT120I GGNCC 2 cut(s) 392, 477
BmrFI CCNGG 1 cut(s) 481
BmsI GCATC 3 cut(s) 152, 166, 268
BplI GAGNNNNNCTC 2 cut(s) 262, 294
BpuEI CTTGAG 1 cut(s) 188
BsaAI YACGTR 1 cut(s) 300
BsaJI CCNNGG 1 cut(s) 384
Bsc4I CCNNNNNNNGG 2 cut(s) 389, 390
BseBI CCWGG 1 cut(s) 481
BseDI CCNNGG 1 cut(s) 384
BseGI GGATG 3 cut(s) 58, 259, 394
BseLI CCNNNNNNNGG 2 cut(s) 389, 390
BsgI GTGCAG 1 cut(s) 377
BshFI GGCC 1 cut(s) 394
BsiSI CCGG 1 cut(s) 46
BslI CCNNNNNNNGG 2 cut(s) 389, 390
BsmAI GTCTC 1 cut(s) 221
BsnI GGCC 1 cut(s) 394
Bsp1407I TGTACA 1 cut(s) 424
Bsp143I GATC 2 cut(s) 318, 328
Bsp19I CCATGG 1 cut(s) 384
BspACI CCGC 1 cut(s) 347
BspANI GGCC 1 cut(s) 394
BspHI TCATGA 2 cut(s) 142, 508
BspPI GGATC 1 cut(s) 313
BsrGI TGTACA 1 cut(s) 424
BssECI CCNNGG 1 cut(s) 384
BssMI GATC 2 cut(s) 318, 328
BssT1I CCWWGG 1 cut(s) 384
Bst2UI CCWGG 1 cut(s) 481
BstAUI TGTACA 1 cut(s) 424
BstBAI YACGTR 1 cut(s) 300
BstC8I GCNNGC 1 cut(s) 248
BstDEI CTNAG 1 cut(s) 68
BstDSI CCRYGG 1 cut(s) 384
BstF5I GGATG 3 cut(s) 58, 259, 394
BstH2I RGCGCY 1 cut(s) 253
BstHHI GCGC 1 cut(s) 252
BstKTI GATC 2 cut(s) 321, 331
BstMAI GTCTC 1 cut(s) 221
BstMBI GATC 2 cut(s) 318, 328
BstMWI GCNNNNNNNGC 1 cut(s) 256
BstNI CCWGG 1 cut(s) 481
BstSCI CCNGG 1 cut(s) 479
BstX2I RGATCY 1 cut(s) 318
BstXI CCANNNNNNTGG 1 cut(s) 385
BstYI RGATCY 1 cut(s) 318
BsuRI GGCC 1 cut(s) 394
BtgI CCRYGG 1 cut(s) 384
BtsCI GGATG 3 cut(s) 58, 259, 394
Cac8I GCNNGC 1 cut(s) 248
CciI TCATGA 2 cut(s) 142, 508
CfoI GCGC 1 cut(s) 252
Cfr13I GGNCC 2 cut(s) 392, 477
CsiI ACCWGGT 1 cut(s) 479
Csp6I GTAC 3 cut(s) 297, 311, 425
CviAII CATG 6 cut(s) 143, 308, 385, 416, 495, 509
CviJI RGCY 6 cut(s) 18, 49, 155, 246, 394, 514
CviKI_1 RGCY 6 cut(s) 18, 49, 155, 246, 394, 514
CviQI GTAC 3 cut(s) 297, 311, 425
DdeI CTNAG 1 cut(s) 68
DpnI GATC 2 cut(s) 320, 330
DpnII GATC 2 cut(s) 318, 328
Eco130I CCWWGG 1 cut(s) 384
Eco47I GGWCC 1 cut(s) 477
Eco47III AGCGCT 1 cut(s) 251
Eco57I CTGAAG 1 cut(s) 216
EcoRI GAATTC 1 cut(s) 274
EcoRII CCWGG 1 cut(s) 479
EcoT14I CCWWGG 1 cut(s) 384
ErhI CCWWGG 1 cut(s) 384
FaeI CATG 6 cut(s) 146, 311, 388, 419, 498, 512
FatI CATG 6 cut(s) 142, 307, 384, 415, 494, 508
FbaI TGATCA 1 cut(s) 328
FokI GGATG 3 cut(s) 65, 246, 401
FspBI CTAG 1 cut(s) 156
GlaI GCGC 1 cut(s) 251
HaeII RGCGCY 1 cut(s) 253
HaeIII GGCC 1 cut(s) 394
HapII CCGG 1 cut(s) 46
HhaI GCGC 1 cut(s) 252
Hin1II CATG 6 cut(s) 146, 311, 388, 419, 498, 512
Hin6I GCGC 1 cut(s) 250
HinP1I GCGC 1 cut(s) 250
HinfI GANTC 2 cut(s) 92, 501
HpaII CCGG 1 cut(s) 46
Hpy188I TCNGA 4 cut(s) 91, 235, 270, 462
Hpy188III TCNNGA 4 cut(s) 143, 322, 509, 525
HpyAV CCTTC 2 cut(s) 143, 273
HpyCH4IV ACGT 1 cut(s) 299
HpyCH4V TGCA 4 cut(s) 179, 259, 303, 358
HpyF10VI GCNNNNNNNGC 1 cut(s) 256
HpyF3I CTNAG 1 cut(s) 68
HpySE526I ACGT 1 cut(s) 299
Hsp92II CATG 6 cut(s) 146, 311, 388, 419, 498, 512
HspAI GCGC 1 cut(s) 250
Ksp22I TGATCA 1 cut(s) 328
Kzo9I GATC 2 cut(s) 318, 328
LmnI GCTCC 1 cut(s) 152
LpnPI CCDG 7 cut(s) 35, 59, 260, 327, 335, 466, 493
LweI GCATC 3 cut(s) 152, 166, 268
MabI ACCWGGT 1 cut(s) 479
MaeI CTAG 1 cut(s) 156
MaeII ACGT 1 cut(s) 299
MalI GATC 2 cut(s) 320, 330
MboI GATC 2 cut(s) 318, 328
MboII GAAGA 3 cut(s) 158, 171, 475
MflI RGATCY 1 cut(s) 318
MluCI AATT 7 cut(s) 85, 97, 180, 202, 274, 285, 446
MnlI CCTC 6 cut(s) 7, 20, 24, 163, 264, 331
MslI CAYNNNNRTG 1 cut(s) 383
MspI CCGG 1 cut(s) 46
MspR9I CCNGG 1 cut(s) 481
MvaI CCWGG 1 cut(s) 481
MwoI GCNNNNNNNGC 1 cut(s) 256
NcoI CCATGG 1 cut(s) 384
NdeII GATC 2 cut(s) 318, 328
NlaIII CATG 6 cut(s) 146, 311, 388, 419, 498, 512
PagI TCATGA 2 cut(s) 142, 508
PfeI GAWTC 2 cut(s) 92, 501
Ppu21I YACGTR 1 cut(s) 300
Psp6I CCWGG 1 cut(s) 479
PspGI CCWGG 1 cut(s) 479
PspPI GGNCC 2 cut(s) 392, 477
PsuI RGATCY 1 cut(s) 318
RsaI GTAC 3 cut(s) 298, 312, 426
RsaNI GTAC 3 cut(s) 297, 311, 425
RseI CAYNNNNRTG 1 cut(s) 383
Sau3AI GATC 2 cut(s) 318, 328
Sau96I GGNCC 2 cut(s) 392, 477
ScrFI CCNGG 1 cut(s) 481
SetI ASST 7 cut(s) 35, 157, 174, 302, 342, 485, 516
SexAI ACCWGGT 1 cut(s) 479
SfaNI GCATC 3 cut(s) 152, 166, 268
SinI GGWCC 1 cut(s) 477
SmiMI CAYNNNNRTG 1 cut(s) 383
SmlI CTYRAG 1 cut(s) 167
SmoI CTYRAG 1 cut(s) 167
Sse9I AATT 7 cut(s) 85, 97, 180, 202, 274, 285, 446
SsiI CCGC 1 cut(s) 347
SspI AATATT 1 cut(s) 193
SspMI CTAG 1 cut(s) 156
StyD4I CCNGG 1 cut(s) 479
StyI CCWWGG 1 cut(s) 384
TaiI ACGT 1 cut(s) 302
TaqI TCGA 2 cut(s) 25, 439
TaqII GACCGA 2 cut(s) 72, 504
TasI AATT 7 cut(s) 85, 97, 180, 202, 274, 285, 446
TatI WGTACW 1 cut(s) 424
TfiI GAWTC 2 cut(s) 92, 501
TspDTI ATGAA 5 cut(s) 10, 20, 131, 159, 497
VpaK11BI GGWCC 1 cut(s) 477
XapI RAATTY 3 cut(s) 85, 274, 446
XspI CTAG 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.