pycom02g21000

Pyruvate kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
N/A
Physical Location & Seq
Reverse (-)
19071944 .. 19075781
3838 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 681 bp
ATGGCTGGAAAGCCAGCAGTGGTGACTCGGGTTGTGGACAGTATGACCGACAACCTAAGACCTACTCGTGCTGAAGCAACTGATGTTGCCAACGCTGTACTGGATGGAACTGATGCAATTGTTCTAGGTGCAGAAACCCTGCGCGGTTTGTACCCTGTTGAGACGATCTCCATTGTTGGAAAGATTTGCTCTGAGGCGGAGAAAGTTTTCAATCAAGATTTGTATTTTAAGAGGACTGTCAAATATGTTGGAGAACCAATGACTCACTTGGAATCTATTGCTTCCTCTGCGGTACGTGCAGCCATCAAGGTGAAGGCCTCTCTCATTATTTGCTTCACTTCATCTGGAAGAGCAGCAAGGCTGATTGCAAAGTACAGACCAACAATGCCTGTCCTTTCTGTTGTCATTCCTCGGGTCCAGACAAATCAACTCCGATGGAGATTTTCTGGTGCATTTGAGGCAAGGCAATCACTCATTGTAAGAGGCCTTTTTCCCACGCTAGCAGATCCTCGACATCCAGCTGGAACTACGGGTGCAACAAATGAGTCGATTCTTAAAATCGCTTTGGATCATGGCAGGGTCGCTGGTGTCGTAAAATCACATGATCGTGTCGTAATTTGCCAGAAAGTTGGCGATGCTTCTGTGATCAAAAGTCAACCGTCGATCGATCATCGAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0004743 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005975 GO:0006082 GO:0006090 GO:0006091 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009987 GO:0016052 GO:0016053 GO:0016310 GO:0016740 GO:0016772 GO:0016773 GO:0017144 GO:0018130 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0042866 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0051186 GO:0051188 GO:0055086 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00230 ko00620 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 ko04922 ko04930 ko05165 ko05203 ko05230 map00010 map00230 map00620 map01100 map01110 map01120 map01130 map01200 map01230 map04922 map04930 map05165 map05203 map05230
Pfam Domains
Protein Families
PK PK_C

Protein Analysis

227

Amino Acids

24.39

Weight (kDa)

9.81

Isoelectric Point (pI)

21.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PK PF00224 2 - 56 1.6e-17 Pyruvate kinase, barrel domain
PK_C PF02887 91 - 210 1.6e-21 Pyruvate kinase, alpha/beta domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 144
AciI CCGC 3 cut(s) 144, 197, 290
AclWI GGATC 2 cut(s) 500, 576
AcuI CTGAAG 1 cut(s) 93
AfaI GTAC 4 cut(s) 99, 152, 294, 374
AgsI TTSAA 1 cut(s) 211
AluBI AGCT 2 cut(s) 521, 677
AluI AGCT 2 cut(s) 521, 677
Alw26I GTCTC 1 cut(s) 155
AlwI GGATC 2 cut(s) 500, 576
Ama87I CYCGRG 2 cut(s) 27, 411
AoxI GGCC 2 cut(s) 315, 484
ApeKI GCWGC 2 cut(s) 299, 353
Asp700I GAANNNNTTC 1 cut(s) 206
AspLEI GCGC 1 cut(s) 144
AspS9I GGNCC 1 cut(s) 415
AsuHPI GGTGA 2 cut(s) 34, 322
AsuNHI GCTAGC 1 cut(s) 499
AvaI CYCGRG 2 cut(s) 27, 411
AvaII GGWCC 1 cut(s) 415
BauI CACGAG 1 cut(s) 66
BbvI GCAGC 2 cut(s) 311, 365
BccI CCATC 3 cut(s) 98, 311, 429
BclI TGATCA 1 cut(s) 645
BcoDI GTCTC 1 cut(s) 155
BfaI CTAG 2 cut(s) 125, 500
BisI GCNGC 2 cut(s) 300, 354
BlsI GCNGC 2 cut(s) 301, 355
Bme18I GGWCC 1 cut(s) 415
BmeT110I CYCGRG 2 cut(s) 27, 411
BmgT120I GGNCC 1 cut(s) 415
BmiI GGNNCC 1 cut(s) 416
BmsI GCATC 2 cut(s) 103, 625
BmtI GCTAGC 1 cut(s) 503
BplI GAGNNNNNCTC 2 cut(s) 152, 184
Bsa29I ATCGAT 1 cut(s) 666
BsaAI YACGTR 1 cut(s) 296
BsaJI CCNNGG 1 cut(s) 410
Bse1I ACTGG 1 cut(s) 105
BseCI ATCGAT 1 cut(s) 666
BseDI CCNNGG 1 cut(s) 410
BseGI GGATG 2 cut(s) 109, 514
BseMII CTCAG 1 cut(s) 183
BseNI ACTGG 1 cut(s) 105
BseXI GCAGC 2 cut(s) 311, 365
BsgI GTGCAG 2 cut(s) 150, 318
Bsh1236I CGCG 1 cut(s) 144
Bsh1285I CGRYCG 1 cut(s) 666
BshFI GGCC 2 cut(s) 317, 486
BshVI ATCGAT 1 cut(s) 666
BsiEI CGRYCG 1 cut(s) 666
BsiHKCI CYCGRG 2 cut(s) 27, 411
BsmAI GTCTC 1 cut(s) 155
BsmBI CGTCTC 1 cut(s) 155
BsnI GGCC 2 cut(s) 317, 486
BsoBI CYCGRG 2 cut(s) 27, 411
Bsp143I GATC 7 cut(s) 165, 505, 568, 604, 645, 663, 667
BspACI CCGC 3 cut(s) 144, 197, 290
BspANI GGCC 2 cut(s) 317, 486
BspCNI CTCAG 1 cut(s) 184
BspDI ATCGAT 1 cut(s) 666
BspFNI CGCG 1 cut(s) 144
BspLI GGNNCC 1 cut(s) 416
BspOI GCTAGC 1 cut(s) 503
BspPI GGATC 2 cut(s) 500, 576
BspQI GCTCTTC 1 cut(s) 343
BsrI ACTGG 1 cut(s) 105
BssECI CCNNGG 1 cut(s) 410
BssMI GATC 7 cut(s) 165, 505, 568, 604, 645, 663, 667
BssSI CACGAG 1 cut(s) 66
Bst2BI CACGAG 1 cut(s) 66
Bst4CI ACNGT 3 cut(s) 41, 238, 660
Bst6I CTCTTC 1 cut(s) 343
BstBAI YACGTR 1 cut(s) 296
BstC8I GCNNGC 2 cut(s) 15, 501
BstDEI CTNAG 2 cut(s) 56, 192
BstF5I GGATG 2 cut(s) 109, 514
BstFNI CGCG 1 cut(s) 144
BstHHI GCGC 1 cut(s) 144
BstKTI GATC 7 cut(s) 168, 508, 571, 607, 648, 666, 670
BstMAI GTCTC 1 cut(s) 155
BstMBI GATC 7 cut(s) 165, 505, 568, 604, 645, 663, 667
BstMCI CGRYCG 1 cut(s) 666
BstMWI GCNNNNNNNGC 3 cut(s) 287, 296, 458
BstUI CGCG 1 cut(s) 144
BstV1I GCAGC 2 cut(s) 311, 365
BstX2I RGATCY 1 cut(s) 505
BstXI CCANNNNNNTGG 1 cut(s) 629
BstYI RGATCY 1 cut(s) 505
Bsu15I ATCGAT 1 cut(s) 666
BsuRI GGCC 2 cut(s) 317, 486
BsuTUI ATCGAT 1 cut(s) 666
BtgZI GCGATG 1 cut(s) 648
BtsCI GGATG 2 cut(s) 109, 514
BtsI GCAGTG 1 cut(s) 24
BtsIMutI CAGTG 1 cut(s) 24
Cac8I GCNNGC 2 cut(s) 15, 501
CfoI GCGC 1 cut(s) 144
Cfr13I GGNCC 1 cut(s) 415
ClaI ATCGAT 1 cut(s) 666
Csp6I GTAC 4 cut(s) 98, 151, 293, 373
CviAII CATG 2 cut(s) 572, 602
CviJI RGCY 8 cut(s) 5, 13, 302, 317, 361, 486, 521, 677
CviKI_1 RGCY 8 cut(s) 5, 13, 302, 317, 361, 486, 521, 677
CviQI GTAC 4 cut(s) 98, 151, 293, 373
DdeI CTNAG 2 cut(s) 56, 192
DpnI GATC 7 cut(s) 167, 507, 570, 606, 647, 665, 669
DpnII GATC 7 cut(s) 165, 505, 568, 604, 645, 663, 667
Eam1104I CTCTTC 1 cut(s) 343
EarI CTCTTC 1 cut(s) 343
EciI GGCGGA 1 cut(s) 212
Eco147I AGGCCT 2 cut(s) 317, 486
Eco47I GGWCC 1 cut(s) 415
Eco57I CTGAAG 1 cut(s) 93
Eco88I CYCGRG 2 cut(s) 27, 411
Esp3I CGTCTC 1 cut(s) 155
FaeI CATG 2 cut(s) 575, 605
FaiI YATR 4 cut(s) 44, 246, 573, 603
FatI CATG 2 cut(s) 571, 601
FbaI TGATCA 1 cut(s) 645
Fnu4HI GCNGC 2 cut(s) 300, 354
FokI GGATG 2 cut(s) 116, 501
Fsp4HI GCNGC 2 cut(s) 300, 354
FspBI CTAG 2 cut(s) 125, 500
GlaI GCGC 1 cut(s) 143
GluI GCNGC 2 cut(s) 300, 354
HaeIII GGCC 2 cut(s) 317, 486
HhaI GCGC 1 cut(s) 144
Hin1II CATG 2 cut(s) 575, 605
Hin6I GCGC 1 cut(s) 142
HinP1I GCGC 1 cut(s) 142
HincII GTYRAC 1 cut(s) 656
HindII GTYRAC 1 cut(s) 656
HinfI GANTC 5 cut(s) 25, 262, 272, 545, 550
HphI GGTGA 2 cut(s) 34, 322
Hpy166II GTNNAC 2 cut(s) 37, 656
Hpy188I TCNGA 2 cut(s) 193, 434
Hpy188III TCNNGA 3 cut(s) 215, 345, 418
Hpy8I GTNNAC 2 cut(s) 37, 656
Hpy99I CGWCG 1 cut(s) 664
HpyAV CCTTC 1 cut(s) 307
HpyCH4III ACNGT 3 cut(s) 41, 238, 660
HpyCH4IV ACGT 1 cut(s) 295
HpyCH4V TGCA 6 cut(s) 116, 131, 299, 368, 452, 536
HpyF10VI GCNNNNNNNGC 3 cut(s) 287, 296, 458
HpyF3I CTNAG 2 cut(s) 56, 192
HpySE526I ACGT 1 cut(s) 295
Hsp92II CATG 2 cut(s) 575, 605
HspAI GCGC 1 cut(s) 142
Ksp22I TGATCA 1 cut(s) 645
Kzo9I GATC 7 cut(s) 165, 505, 568, 604, 645, 663, 667
LguI GCTCTTC 1 cut(s) 343
Lsp1109I GCAGC 2 cut(s) 311, 365
LweI GCATC 2 cut(s) 103, 625
MaeI CTAG 2 cut(s) 125, 500
MaeII ACGT 1 cut(s) 295
MaeIII GTNAC 1 cut(s) 22
MalI GATC 7 cut(s) 167, 507, 570, 606, 647, 665, 669
MboI GATC 7 cut(s) 165, 505, 568, 604, 645, 663, 667
MboII GAAGA 1 cut(s) 360
MfeI CAATTG 1 cut(s) 117
MflI RGATCY 1 cut(s) 505
MluCI AATT 2 cut(s) 117, 615
MlyI GAGTC 3 cut(s) 19, 256, 554
MmeI TCCRAC 2 cut(s) 157, 229
MnlI CCTC 8 cut(s) 187, 225, 295, 328, 420, 451, 476, 519
MroXI GAANNNNTTC 1 cut(s) 206
MseI TTAA 2 cut(s) 228, 555
MslI CAYNNNNRTG 2 cut(s) 308, 606
MspA1I CMGCKG 1 cut(s) 521
MunI CAATTG 1 cut(s) 117
MvnI CGCG 1 cut(s) 144
MwoI GCNNNNNNNGC 3 cut(s) 287, 296, 458
NdeII GATC 7 cut(s) 165, 505, 568, 604, 645, 663, 667
NheI GCTAGC 1 cut(s) 499
NlaIII CATG 2 cut(s) 575, 605
NlaIV GGNNCC 1 cut(s) 416
NmuCI GTSAC 1 cut(s) 22
PceI AGGCCT 2 cut(s) 317, 486
PciSI GCTCTTC 1 cut(s) 343
PcsI WCGNNNNNNNCGW 1 cut(s) 588
PdmI GAANNNNTTC 1 cut(s) 206
PfeI GAWTC 2 cut(s) 272, 550
PkrI GCNGC 2 cut(s) 301, 355
Ple19I CGATCG 1 cut(s) 666
PleI GAGTC 3 cut(s) 19, 256, 553
PpsI GAGTC 3 cut(s) 19, 256, 553
Ppu21I YACGTR 1 cut(s) 296
PspN4I GGNNCC 1 cut(s) 416
PspPI GGNCC 1 cut(s) 415
PsuI RGATCY 1 cut(s) 505
PvuI CGATCG 1 cut(s) 666
PvuII CAGCTG 1 cut(s) 521
RsaI GTAC 4 cut(s) 99, 152, 294, 374
RsaNI GTAC 4 cut(s) 98, 151, 293, 373
RseI CAYNNNNRTG 2 cut(s) 308, 606
SapI GCTCTTC 1 cut(s) 343
SaqAI TTAA 2 cut(s) 228, 555
SatI GCNGC 2 cut(s) 300, 354
Sau3AI GATC 7 cut(s) 165, 505, 568, 604, 645, 663, 667
Sau96I GGNCC 1 cut(s) 415
SchI GAGTC 3 cut(s) 19, 256, 554
SetI ASST 7 cut(s) 57, 64, 130, 298, 312, 523, 679
SfaNI GCATC 2 cut(s) 103, 625
SinI GGWCC 1 cut(s) 415
SmiMI CAYNNNNRTG 2 cut(s) 308, 606
Sse9I AATT 2 cut(s) 117, 615
SseBI AGGCCT 2 cut(s) 317, 486
SsiI CCGC 3 cut(s) 144, 197, 290
SspMI CTAG 2 cut(s) 125, 500
StuI AGGCCT 2 cut(s) 317, 486
TaaI ACNGT 3 cut(s) 41, 238, 660
TaiI ACGT 1 cut(s) 298
TaqI TCGA 5 cut(s) 511, 548, 662, 666, 673
TaqII GACCGA 1 cut(s) 62
TasI AATT 2 cut(s) 117, 615
TatI WGTACW 2 cut(s) 97, 372
TfiI GAWTC 2 cut(s) 272, 550
Tru1I TTAA 2 cut(s) 228, 555
Tru9I TTAA 2 cut(s) 228, 555
TscAI CASTG 1 cut(s) 24
TseFI GTSAC 1 cut(s) 22
TseI GCWGC 2 cut(s) 299, 353
Tsp45I GTSAC 1 cut(s) 22
TspDTI ATGAA 1 cut(s) 330
TspRI CASTG 1 cut(s) 24
VpaK11BI GGWCC 1 cut(s) 415
XcmI CCANNNNNNNNNTGG 1 cut(s) 97
XmnI GAANNNNTTC 1 cut(s) 206
XspI CTAG 2 cut(s) 125, 500
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.