pycom02g23050

Cupin domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
N/A
Physical Location & Seq
Forward (+)
21173750 .. 21174186
437 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 342 bp
ATGGCTTTGAGATTAGTTCTTCAGTTAGTGATCATACTAGTACTTGCCCTGACCATTTCCAAAAAGGGTCCTAGCCGGCGATCGGGGATCCTTTCCGACTTCATAGTTCCACCAAGGTTCGTGGACACGAAATACATTCTATACACTCAAAAGCTTAGAGTTGGAAACCTACTTGTGATTCCTAACGGATTAGTTCATTATCAGTATAACCCAGATGCTCAATTACCAGCCTATGCCATTTCATTTGCCACTGGAATTGACGATCCGATCCTCGCCAAATCATTTAGGACTGAAGTTGGTACCATTGAGAAGATCAAGTCTGGTCTCACCACTCATAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Cupin_1

Protein Analysis

114

Amino Acids

12.53

Weight (kDa)

9.93

Isoelectric Point (pI)

39.37

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 299
AccB1I GGYRCC 1 cut(s) 299
AclWI GGATC 4 cut(s) 82, 95, 257, 262
AcuI CTGAAG 2 cut(s) 5, 312
AfaI GTAC 2 cut(s) 42, 301
AfiI CCNNNNNNNGG 1 cut(s) 82
AhlI ACTAGT 1 cut(s) 37
AluBI AGCT 1 cut(s) 154
AluI AGCT 1 cut(s) 154
Alw26I GTCTC 1 cut(s) 329
AlwI GGATC 4 cut(s) 82, 95, 257, 262
Asp718I GGTACC 1 cut(s) 299
AspS9I GGNCC 1 cut(s) 68
AsuHPI GGTGA 1 cut(s) 319
AvaII GGWCC 1 cut(s) 68
BamHI GGATCC 1 cut(s) 87
BanI GGYRCC 1 cut(s) 299
BclI TGATCA 1 cut(s) 30
BcoDI GTCTC 1 cut(s) 329
BcuI ACTAGT 1 cut(s) 37
BfaI CTAG 2 cut(s) 38, 72
BmcAI AGTACT 1 cut(s) 42
Bme18I GGWCC 1 cut(s) 68
BmgT120I GGNCC 1 cut(s) 68
BmiI GGNNCC 3 cut(s) 69, 89, 301
BmsI GCATC 1 cut(s) 205
BsaI GGTCTC 1 cut(s) 329
BsaJI CCNNGG 1 cut(s) 113
Bsc4I CCNNNNNNNGG 1 cut(s) 82
Bse118I RCCGGY 1 cut(s) 75
Bse1I ACTGG 1 cut(s) 256
BseDI CCNNGG 1 cut(s) 113
BseLI CCNNNNNNNGG 1 cut(s) 82
BseNI ACTGG 1 cut(s) 256
Bsh1285I CGRYCG 1 cut(s) 83
BshNI GGYRCC 1 cut(s) 299
BsiEI CGRYCG 1 cut(s) 83
BsiSI CCGG 1 cut(s) 76
BslI CCNNNNNNNGG 1 cut(s) 82
BsmAI GTCTC 1 cut(s) 329
Bso31I GGTCTC 1 cut(s) 329
Bsp143I GATC 6 cut(s) 30, 80, 87, 262, 267, 312
BspLI GGNNCC 3 cut(s) 69, 89, 301
BspPI GGATC 4 cut(s) 82, 95, 257, 262
BspT107I GGYRCC 1 cut(s) 299
BspTNI GGTCTC 1 cut(s) 329
BsrFI RCCGGY 1 cut(s) 75
BsrI ACTGG 1 cut(s) 256
BssAI RCCGGY 1 cut(s) 75
BssECI CCNNGG 1 cut(s) 113
BssMI GATC 6 cut(s) 30, 80, 87, 262, 267, 312
BssT1I CCWWGG 1 cut(s) 113
BstC8I GCNNGC 1 cut(s) 77
BstDEI CTNAG 1 cut(s) 155
BstKTI GATC 6 cut(s) 33, 83, 90, 265, 270, 315
BstMAI GTCTC 1 cut(s) 329
BstMBI GATC 6 cut(s) 30, 80, 87, 262, 267, 312
BstMCI CGRYCG 1 cut(s) 83
BstX2I RGATCY 1 cut(s) 87
BstYI RGATCY 1 cut(s) 87
BtsIMutI CAGTG 1 cut(s) 249
Cac8I GCNNGC 1 cut(s) 77
Cfr10I RCCGGY 1 cut(s) 75
Cfr13I GGNCC 1 cut(s) 68
Csp6I GTAC 2 cut(s) 41, 300
CspCI CAANNNNNGTGG 4 cut(s) 102, 137, 238, 273
CviJI RGCY 4 cut(s) 5, 75, 154, 230
CviKI_1 RGCY 4 cut(s) 5, 75, 154, 230
CviQI GTAC 2 cut(s) 41, 300
DdeI CTNAG 1 cut(s) 155
DpnI GATC 6 cut(s) 32, 82, 89, 264, 269, 314
DpnII GATC 6 cut(s) 30, 80, 87, 262, 267, 312
Eco130I CCWWGG 1 cut(s) 113
Eco31I GGTCTC 1 cut(s) 329
Eco47I GGWCC 1 cut(s) 68
Eco57I CTGAAG 2 cut(s) 5, 312
EcoO109I RGGNCCY 1 cut(s) 68
EcoT14I CCWWGG 1 cut(s) 113
ErhI CCWWGG 1 cut(s) 113
FaiI YATR 6 cut(s) 35, 104, 142, 207, 234, 336
FbaI TGATCA 1 cut(s) 30
FspBI CTAG 2 cut(s) 38, 72
HapII CCGG 1 cut(s) 76
HindIII AAGCTT 1 cut(s) 152
HinfI GANTC 1 cut(s) 178
HpaII CCGG 1 cut(s) 76
HphI GGTGA 1 cut(s) 319
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 2 cut(s) 97, 267
Hpy8I GTNNAC 1 cut(s) 124
HpyF3I CTNAG 1 cut(s) 155
KpnI GGTACC 1 cut(s) 303
KroI GCCGGC 1 cut(s) 75
KroNI GCCGGC 1 cut(s) 77
Ksp22I TGATCA 1 cut(s) 30
Kzo9I GATC 6 cut(s) 30, 80, 87, 262, 267, 312
LpnPI CCDG 6 cut(s) 62, 89, 225, 237, 240, 306
LweI GCATC 1 cut(s) 205
MaeI CTAG 2 cut(s) 38, 72
MalI GATC 6 cut(s) 32, 82, 89, 264, 269, 314
MboI GATC 6 cut(s) 30, 80, 87, 262, 267, 312
MboII GAAGA 2 cut(s) 11, 322
MflI RGATCY 1 cut(s) 87
MluCI AATT 2 cut(s) 221, 255
MmeI TCCRAC 2 cut(s) 120, 142
MnlI CCTC 1 cut(s) 281
MroNI GCCGGC 1 cut(s) 75
MspI CCGG 1 cut(s) 76
NaeI GCCGGC 1 cut(s) 77
NdeII GATC 6 cut(s) 30, 80, 87, 262, 267, 312
NgoMIV GCCGGC 1 cut(s) 75
NlaIV GGNNCC 3 cut(s) 69, 89, 301
PdiI GCCGGC 1 cut(s) 77
PfeI GAWTC 1 cut(s) 178
Ple19I CGATCG 1 cut(s) 83
PpuMI RGGWCCY 1 cut(s) 68
Psp5II RGGWCCY 1 cut(s) 68
PspN4I GGNNCC 3 cut(s) 69, 89, 301
PspPI GGNCC 1 cut(s) 68
PspPPI RGGWCCY 1 cut(s) 68
PsuI RGATCY 1 cut(s) 87
PvuI CGATCG 1 cut(s) 83
RsaI GTAC 2 cut(s) 42, 301
RsaNI GTAC 2 cut(s) 41, 300
Sau3AI GATC 6 cut(s) 30, 80, 87, 262, 267, 312
Sau96I GGNCC 1 cut(s) 68
ScaI AGTACT 1 cut(s) 42
SetI ASST 3 cut(s) 119, 156, 171
SfaNI GCATC 1 cut(s) 205
SinI GGWCC 1 cut(s) 68
SpeI ACTAGT 1 cut(s) 37
Sse9I AATT 2 cut(s) 221, 255
SspMI CTAG 2 cut(s) 38, 72
StyI CCWWGG 1 cut(s) 113
TasI AATT 2 cut(s) 221, 255
TatI WGTACW 1 cut(s) 40
TfiI GAWTC 1 cut(s) 178
TscAI CASTG 1 cut(s) 256
TspDTI ATGAA 3 cut(s) 91, 185, 231
TspGWI ACGGA 1 cut(s) 201
TspRI CASTG 1 cut(s) 256
VpaK11BI GGWCC 1 cut(s) 68
XspI CTAG 2 cut(s) 38, 72
ZrmI AGTACT 1 cut(s) 42
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.