pycom02g24490

cucumisin-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
N/A
Physical Location & Seq
Forward (+)
22645449 .. 22645962
514 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 375 bp
ATGAATGATACAAGCACTTTCCCAGGCGAATTTGGTTATGGATCTAGACATCTTAATCCTGTAGGAGCTAAGCAGCTAGGACTTGTGTACGATGCTTCTAAAAAAGATTACATTAAGTTGCTTTGCTTGTTTTATATTGTTGAGAGTTACGAATGGTGGCCATGCAAACTCCAGGCCAGTATCTCCAAAAATTCTGAAAATGTTGACATCAAAGCGGTGCCTGAAGTTCTTAACTTCAAATCCTTAAACGAGGTGAAAAGTTCCGTTGTGACCATTGCTGAACAGGCTTTCGTGGCAGGAATTGTGACTGCATCGCTAACCTGGTCTTATGGAAATCACCCGGTTACAAGTCCAATTACTGTACATGGAATGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

125

Amino Acids

13.69

Weight (kDa)

6.26

Isoelectric Point (pI)

19.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
fn3_6 PF17766 60 - 121 3.7e-08 Fibronectin type-III domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 217
AciI CCGC 1 cut(s) 215
AclWI GGATC 1 cut(s) 49
AcoI YGGCCR 1 cut(s) 158
AcsI RAATTY 2 cut(s) 29, 190
AcuI CTGAAG 1 cut(s) 243
AfaI GTAC 2 cut(s) 89, 363
AgsI TTSAA 1 cut(s) 238
AjnI CCWGG 3 cut(s) 22, 171, 320
AluBI AGCT 2 cut(s) 68, 76
AluI AGCT 2 cut(s) 68, 76
AlwI GGATC 1 cut(s) 49
AoxI GGCC 2 cut(s) 158, 174
ApeKI GCWGC 1 cut(s) 73
ApoI RAATTY 2 cut(s) 29, 190
AsuC2I CCSGG 1 cut(s) 341
AsuHPI GGTGA 2 cut(s) 265, 329
BalI TGGCCA 1 cut(s) 160
BanI GGYRCC 1 cut(s) 217
BbvI GCAGC 1 cut(s) 85
BciT130I CCWGG 3 cut(s) 24, 173, 322
BcnI CCSGG 1 cut(s) 341
BfaI CTAG 2 cut(s) 45, 77
BfmI CTRYAG 1 cut(s) 60
BisI GCNGC 1 cut(s) 74
BlpI GCTNAGC 1 cut(s) 69
BlsI GCNGC 1 cut(s) 75
Bme1390I CCNGG 4 cut(s) 24, 173, 322, 341
BmiI GGNNCC 1 cut(s) 219
BmrFI CCNGG 4 cut(s) 24, 173, 322, 341
BmsI GCATC 2 cut(s) 82, 320
BpmI CTGGAG 1 cut(s) 155
Bpu1102I GCTNAGC 1 cut(s) 69
BpuMI CCSGG 1 cut(s) 341
BsaJI CCNNGG 1 cut(s) 22
Bse1I ACTGG 1 cut(s) 177
Bse3DI GCAATG 1 cut(s) 273
BseBI CCWGG 3 cut(s) 24, 173, 322
BseDI CCNNGG 1 cut(s) 22
BseMI GCAATG 1 cut(s) 273
BseNI ACTGG 1 cut(s) 177
BseXI GCAGC 1 cut(s) 85
BshFI GGCC 2 cut(s) 160, 176
BshNI GGYRCC 1 cut(s) 217
BsiSI CCGG 1 cut(s) 341
BsnI GGCC 2 cut(s) 160, 176
Bsp1407I TGTACA 1 cut(s) 361
Bsp143I GATC 1 cut(s) 41
Bsp1720I GCTNAGC 1 cut(s) 69
BspACI CCGC 1 cut(s) 215
BspANI GGCC 2 cut(s) 160, 176
BspLI GGNNCC 1 cut(s) 219
BspPI GGATC 1 cut(s) 49
BspT107I GGYRCC 1 cut(s) 217
BsrDI GCAATG 1 cut(s) 273
BsrGI TGTACA 1 cut(s) 361
BsrI ACTGG 1 cut(s) 177
BssECI CCNNGG 1 cut(s) 22
BssMI GATC 1 cut(s) 41
Bst2UI CCWGG 3 cut(s) 24, 173, 322
Bst4CI ACNGT 1 cut(s) 361
BstAUI TGTACA 1 cut(s) 361
BstDEI CTNAG 1 cut(s) 69
BstKTI GATC 1 cut(s) 44
BstMBI GATC 1 cut(s) 41
BstMWI GCNNNNNNNGC 2 cut(s) 284, 293
BstNI CCWGG 3 cut(s) 24, 173, 322
BstSCI CCNGG 4 cut(s) 22, 171, 320, 339
BstSFI CTRYAG 1 cut(s) 60
BstV1I GCAGC 1 cut(s) 85
BstX2I RGATCY 1 cut(s) 41
BstYI RGATCY 1 cut(s) 41
BsuRI GGCC 2 cut(s) 160, 176
BtgZI GCGATG 1 cut(s) 297
CsiI ACCWGGT 1 cut(s) 320
Csp6I GTAC 2 cut(s) 88, 362
CviAII CATG 2 cut(s) 162, 365
CviJI RGCY 5 cut(s) 68, 76, 160, 176, 287
CviKI_1 RGCY 5 cut(s) 68, 76, 160, 176, 287
CviQI GTAC 2 cut(s) 88, 362
DdeI CTNAG 1 cut(s) 69
DpnI GATC 1 cut(s) 43
DpnII GATC 1 cut(s) 41
EaeI YGGCCR 1 cut(s) 158
Eco57I CTGAAG 1 cut(s) 243
EcoRII CCWGG 3 cut(s) 22, 171, 320
FaeI CATG 2 cut(s) 165, 368
FaiI YATR 5 cut(s) 39, 135, 163, 330, 366
FatI CATG 2 cut(s) 161, 364
Fnu4HI GCNGC 1 cut(s) 74
Fsp4HI GCNGC 1 cut(s) 74
FspBI CTAG 2 cut(s) 45, 77
GluI GCNGC 1 cut(s) 74
GsuI CTGGAG 1 cut(s) 155
HaeIII GGCC 2 cut(s) 160, 176
HapII CCGG 1 cut(s) 341
Hin1II CATG 2 cut(s) 165, 368
HincII GTYRAC 1 cut(s) 205
HindII GTYRAC 1 cut(s) 205
HpaII CCGG 1 cut(s) 341
HphI GGTGA 2 cut(s) 265, 329
Hpy166II GTNNAC 2 cut(s) 88, 205
Hpy188I TCNGA 1 cut(s) 196
Hpy188III TCNNGA 1 cut(s) 45
Hpy8I GTNNAC 2 cut(s) 88, 205
HpyCH4III ACNGT 1 cut(s) 361
HpyCH4V TGCA 2 cut(s) 165, 311
HpyF10VI GCNNNNNNNGC 2 cut(s) 284, 293
HpyF3I CTNAG 1 cut(s) 69
Hsp92II CATG 2 cut(s) 165, 368
Kzo9I GATC 1 cut(s) 41
LmnI GCTCC 1 cut(s) 65
Lsp1109I GCAGC 1 cut(s) 85
LweI GCATC 2 cut(s) 82, 320
MabI ACCWGGT 1 cut(s) 320
MaeI CTAG 2 cut(s) 45, 77
MaeIII GTNAC 4 cut(s) 146, 268, 304, 343
MalI GATC 1 cut(s) 43
MboI GATC 1 cut(s) 41
MflI RGATCY 1 cut(s) 41
MlsI TGGCCA 1 cut(s) 160
MluCI AATT 4 cut(s) 29, 190, 300, 354
MluNI TGGCCA 1 cut(s) 160
MnlI CCTC 1 cut(s) 244
Mox20I TGGCCA 1 cut(s) 160
MscI TGGCCA 1 cut(s) 160
MseI TTAA 4 cut(s) 54, 114, 231, 245
Msp20I TGGCCA 1 cut(s) 160
MspI CCGG 1 cut(s) 341
MspR9I CCNGG 4 cut(s) 24, 173, 322, 341
MvaI CCWGG 3 cut(s) 24, 173, 322
MwoI GCNNNNNNNGC 2 cut(s) 284, 293
NciI CCSGG 1 cut(s) 341
NdeII GATC 1 cut(s) 41
NlaIII CATG 2 cut(s) 165, 368
NlaIV GGNNCC 1 cut(s) 219
NmuCI GTSAC 2 cut(s) 268, 304
PkrI GCNGC 1 cut(s) 75
Psp6I CCWGG 3 cut(s) 22, 171, 320
PspGI CCWGG 3 cut(s) 22, 171, 320
PspN4I GGNNCC 1 cut(s) 219
PsuI RGATCY 1 cut(s) 41
RsaI GTAC 2 cut(s) 89, 363
RsaNI GTAC 2 cut(s) 88, 362
SaqAI TTAA 4 cut(s) 54, 114, 231, 245
SatI GCNGC 1 cut(s) 74
Sau3AI GATC 1 cut(s) 41
ScrFI CCNGG 4 cut(s) 24, 173, 322, 341
SetI ASST 4 cut(s) 70, 78, 255, 323
SexAI ACCWGGT 1 cut(s) 320
SfaNI GCATC 2 cut(s) 82, 320
SfcI CTRYAG 1 cut(s) 60
Sse9I AATT 4 cut(s) 29, 190, 300, 354
SsiI CCGC 1 cut(s) 215
SspMI CTAG 2 cut(s) 45, 77
StyD4I CCNGG 4 cut(s) 22, 171, 320, 339
TaaI ACNGT 1 cut(s) 361
TasI AATT 4 cut(s) 29, 190, 300, 354
TatI WGTACW 1 cut(s) 361
Tru1I TTAA 4 cut(s) 54, 114, 231, 245
Tru9I TTAA 4 cut(s) 54, 114, 231, 245
TseFI GTSAC 2 cut(s) 268, 304
TseI GCWGC 1 cut(s) 73
Tsp45I GTSAC 2 cut(s) 268, 304
TspDTI ATGAA 1 cut(s) 17
TspGWI ACGGA 1 cut(s) 253
XapI RAATTY 2 cut(s) 29, 190
XbaI TCTAGA 1 cut(s) 44
XspI CTAG 2 cut(s) 45, 77
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.