pycom02g25580

dihydrokaempferol 4-reductase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
N/A
Physical Location & Seq
Forward (+)
23435039 .. 23437860
2822 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1281 bp
ATGTATGTCCAATGGGGTGGTTGTAATCCTAGTTCAACTCACCAGTTCTGTTTGTGTAATGCCACAGATGATCGATCAAAGAAAACAGGGCACTTGCTCTCACTTGATGGAGCAAAAGAAAGGCTTCATTTGTTCAAGGCAGGTTTATTAGAAGAAGGAGCTTTCGACCCCGTAATTGATGGATGTGTTGGTGTTTTTCATACAGCATCGCCTGTACAACTTTCAGCCACTGACCCACAGGCAGAAATAGTTGAGCCTGCAGTGAAGGGAACTCTGAATGTTCAAAAATCATGTGTGAAATTTCCCACTGTCAAGAGAGTGGTTTTAACATCTTCTATGTCTTCAGTATCGCATAGTAGAACACCGGTAACTTCTGAGATGGTTTTGGATGAAACATGGCACTCAGATCCACTTGTTTGTGGGCAGAACAAGGTAGATGATCCAAAGAAAACAGAGCACTTGCTCTCACTTGATGGAGCAAAAGAAAGGCTTCATTTGTTCAAGGCAGGTTTATTAGAAGAAGGAGCTTTCGACCCCGTAATTGATGGATGTGTTGGTGTTTTTCATACAGCATCGCCTGTACAACTTTCAGCCACTGACCCACAGGCAGAAATAGTTGAGCCTGCAGTGAAGGGAACTCTGAATGTTCTAAAATCATGTGTGAAATTTCCCACTGTCAAGAGAGTGGTTTTAACATCTTCTATGTCTTCAGTATCGTTTAGTAGAACACCGATAACTTCTGATATGGTTTTGGATGAAACATGGTACTCAGATCCACTTGTTTGTGAGCAGAACCAGAGATGGTATCCGCTCTCCAAAATTTTAGCTGAGGAGGCTGCCTGGAAAGTTGCAAAAGAAAATGGGATCGACTTGGTGTCGATGAATCCAGGGATTGTGATTGGTCCGCTCTTGCAGCCAACTCTTAATGGCTCCGTGGAGCTGATTGCGAATATCATAAGTGGTATCCAAACACCATTTCTAAATTATACATTTGTAGACGTTAGAGATGTTGCCTCTGCCCATATTCAAGCATTCGAAGTTCCTTCAGCTAATGGCAGATATTGTTTAGTTGGCCAAGTAGCTGATGGCCCCGATACTCTACAGATTTTACGACAACTTTACCCCACTTTGTGCCTTCCTGAGTTTGGCAATCCTTCTGACTCAAAGTATCAAGTGTCCAGGGAGAAAGCGAAAAGTTTGGGAATTAATTTCCTTCCTCTGGAAGAAAGTCTTAGGGACACTATTGAAAGCCTCAAGGAGAAGGGCTTCCTCAATATCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Epimerase

Protein Analysis

427

Amino Acids

46.42

Weight (kDa)

5.36

Isoelectric Point (pI)

38.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
3Beta_HSD PF01073 49 - 118 7e-06 3-beta hydroxysteroid dehydrogenase/isomerase family
3Beta_HSD PF01073 152 - 345 1.5e-11 3-beta hydroxysteroid dehydrogenase/isomerase family
Epimerase PF01370 160 - 350 2.9e-13 NAD dependent epimerase/dehydratase family
NAD_binding_4 PF07993 173 - 323 1.3e-07 Male sterility protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 131, 497
AccBSI CCGCTC 2 cut(s) 811, 907
AccI GTMKAC 1 cut(s) 996
AciI CCGC 2 cut(s) 809, 905
AclWI GGATC 4 cut(s) 401, 434, 767, 872
AcoI YGGCCR 1 cut(s) 1072
AcsI RAATTY 3 cut(s) 299, 665, 819
AcuI CTGAAG 3 cut(s) 327, 693, 1029
AdeI CACNNNGTG 1 cut(s) 1131
AfaI GTAC 3 cut(s) 216, 582, 767
AfiI CCNNNNNNNGG 2 cut(s) 1145, 1219
AgeI ACCGGT 1 cut(s) 364
AgsI TTSAA 6 cut(s) 36, 136, 284, 502, 1028, 1247
AhdI GACNNNNNGTC 1 cut(s) 874
AjnI CCWGG 3 cut(s) 839, 886, 1178
AjuI GAANNNNNNNTTGG 2 cut(s) 960, 992
AluBI AGCT 6 cut(s) 161, 527, 827, 940, 1049, 1082
AluI AGCT 6 cut(s) 161, 527, 827, 940, 1049, 1082
Alw21I GWGCWC 1 cut(s) 459
AlwI GGATC 4 cut(s) 401, 434, 767, 872
AlwNI CAGNNNCTG 2 cut(s) 230, 596
AoxI GGCC 2 cut(s) 1072, 1087
ApeKI GCWGC 2 cut(s) 836, 913
ApoI RAATTY 3 cut(s) 299, 665, 819
AseI ATTAAT 1 cut(s) 1206
AsiGI ACCGGT 1 cut(s) 364
Asp700I GAANNNNTTC 3 cut(s) 123, 489, 1265
AspS9I GGNCC 2 cut(s) 902, 1088
AsuHPI GGTGA 1 cut(s) 32
AsuII TTCGAA 1 cut(s) 1035
AvaII GGWCC 1 cut(s) 902
BaeGI GKGCMC 1 cut(s) 93
BalI TGGCCA 1 cut(s) 1074
BbsI GAAGAC 2 cut(s) 333, 699
Bbv12I GWGCWC 1 cut(s) 459
BbvCI CCTCAGC 1 cut(s) 828
BbvI GCAGC 2 cut(s) 823, 925
BccI CCATC 7 cut(s) 101, 173, 373, 467, 539, 795, 1079
BciT130I CCWGG 3 cut(s) 841, 888, 1180
BciVI GTATCC 2 cut(s) 816, 974
BfaI CTAG 1 cut(s) 30
BfmI CTRYAG 3 cut(s) 258, 624, 1100
BfuAI ACCTGC 2 cut(s) 131, 497
BfuI GTATCC 2 cut(s) 816, 974
BisI GCNGC 2 cut(s) 837, 914
BlsI GCNGC 2 cut(s) 838, 915
Bme1390I CCNGG 3 cut(s) 841, 888, 1180
Bme18I GGWCC 1 cut(s) 902
BmeRI GACNNNNNGTC 1 cut(s) 874
BmgT120I GGNCC 2 cut(s) 902, 1088
BmiI GGNNCC 2 cut(s) 931, 1090
BmrFI CCNGG 3 cut(s) 841, 888, 1180
BmsI GCATC 2 cut(s) 215, 581
BpiI GAAGAC 2 cut(s) 333, 699
Bpu10I CCTNAGC 1 cut(s) 828
Bpu14I TTCGAA 1 cut(s) 1035
BpuEI CTTGAG 1 cut(s) 1238
Bsa29I ATCGAT 1 cut(s) 73
BsaJI CCNNGG 3 cut(s) 887, 933, 1179
BsaWI WCCGGW 1 cut(s) 364
Bsc4I CCNNNNNNNGG 2 cut(s) 1145, 1219
Bse118I RCCGGY 1 cut(s) 364
Bse1I ACTGG 1 cut(s) 43
BseBI CCWGG 3 cut(s) 841, 888, 1180
BseCI ATCGAT 1 cut(s) 73
BseDI CCNNGG 3 cut(s) 887, 933, 1179
BseGI GGATG 4 cut(s) 188, 394, 554, 760
BseLI CCNNNNNNNGG 2 cut(s) 1145, 1219
BseMII CTCAG 5 cut(s) 366, 417, 783, 819, 1131
BseNI ACTGG 1 cut(s) 43
BseRI GAGGAG 1 cut(s) 845
BseSI GKGCMC 1 cut(s) 93
BseXI GCAGC 2 cut(s) 823, 925
BshFI GGCC 2 cut(s) 1074, 1089
BshTI ACCGGT 1 cut(s) 364
BshVI ATCGAT 1 cut(s) 73
BsiHKAI GWGCWC 1 cut(s) 459
BsiSI CCGG 1 cut(s) 365
BslFI GGGAC 1 cut(s) 1250
BslI CCNNNNNNNGG 2 cut(s) 1145, 1219
BsmFI GGGAC 1 cut(s) 1250
BsmI GAATGC 1 cut(s) 1031
BsnI GGCC 2 cut(s) 1074, 1089
Bsp119I TTCGAA 1 cut(s) 1035
Bsp1286I GDGCHC 2 cut(s) 93, 459
Bsp1407I TGTACA 2 cut(s) 214, 580
Bsp143I GATC 6 cut(s) 70, 74, 406, 439, 772, 864
BspACI CCGC 2 cut(s) 809, 905
BspANI GGCC 2 cut(s) 1074, 1089
BspCNI CTCAG 5 cut(s) 367, 416, 782, 820, 1132
BspDI ATCGAT 1 cut(s) 73
BspLI GGNNCC 2 cut(s) 931, 1090
BspMAI CTGCAG 2 cut(s) 262, 628
BspMI ACCTGC 2 cut(s) 131, 497
BspPI GGATC 4 cut(s) 401, 434, 767, 872
BspT104I TTCGAA 1 cut(s) 1035
BsrBI CCGCTC 2 cut(s) 811, 907
BsrFI RCCGGY 1 cut(s) 364
BsrGI TGTACA 2 cut(s) 214, 580
BsrI ACTGG 1 cut(s) 43
BssAI RCCGGY 1 cut(s) 364
BssECI CCNNGG 3 cut(s) 887, 933, 1179
BssMI GATC 6 cut(s) 70, 74, 406, 439, 772, 864
Bst2UI CCWGG 3 cut(s) 841, 888, 1180
Bst4CI ACNGT 2 cut(s) 310, 676
BstAUI TGTACA 2 cut(s) 214, 580
BstBI TTCGAA 1 cut(s) 1035
BstC8I GCNNGC 2 cut(s) 258, 624
BstDEI CTNAG 6 cut(s) 375, 403, 769, 828, 1140, 1232
BstDSI CCRYGG 1 cut(s) 933
BstF5I GGATG 4 cut(s) 188, 394, 554, 760
BstKTI GATC 6 cut(s) 73, 77, 409, 442, 775, 867
BstMBI GATC 6 cut(s) 70, 74, 406, 439, 772, 864
BstMWI GCNNNNNNNGC 2 cut(s) 833, 913
BstNI CCWGG 3 cut(s) 841, 888, 1180
BstSCI CCNGG 3 cut(s) 839, 886, 1178
BstSFI CTRYAG 3 cut(s) 258, 624, 1100
BstSLI GKGCMC 1 cut(s) 93
BstV1I GCAGC 2 cut(s) 823, 925
BstV2I GAAGAC 2 cut(s) 333, 699
BstX2I RGATCY 2 cut(s) 406, 772
BstXI CCANNNNNNTGG 1 cut(s) 17
BstYI RGATCY 2 cut(s) 406, 772
Bsu15I ATCGAT 1 cut(s) 73
BsuI GTATCC 2 cut(s) 816, 974
BsuRI GGCC 2 cut(s) 1074, 1089
BsuTUI ATCGAT 1 cut(s) 73
BtgI CCRYGG 1 cut(s) 933
BtgZI GCGATG 2 cut(s) 192, 558
BtsCI GGATG 4 cut(s) 188, 394, 554, 760
BtsI GCAGTG 2 cut(s) 267, 633
BtsIMutI CAGTG 6 cut(s) 228, 267, 306, 594, 633, 672
BveI ACCTGC 2 cut(s) 131, 497
Cac8I GCNNGC 2 cut(s) 258, 624
CaiI CAGNNNCTG 2 cut(s) 230, 596
Cfr10I RCCGGY 1 cut(s) 364
Cfr13I GGNCC 2 cut(s) 902, 1088
ClaI ATCGAT 1 cut(s) 73
Csp6I GTAC 3 cut(s) 215, 581, 766
CspAI ACCGGT 1 cut(s) 364
CviAII CATG 4 cut(s) 291, 396, 657, 762
CviQI GTAC 3 cut(s) 215, 581, 766
DdeI CTNAG 6 cut(s) 375, 403, 769, 828, 1140, 1232
DpnI GATC 6 cut(s) 72, 76, 408, 441, 774, 866
DpnII GATC 6 cut(s) 70, 74, 406, 439, 772, 864
DraIII CACNNNGTG 1 cut(s) 1131
DriI GACNNNNNGTC 1 cut(s) 874
EaeI YGGCCR 1 cut(s) 1072
Eam1105I GACNNNNNGTC 1 cut(s) 874
Eco47I GGWCC 1 cut(s) 902
Eco57I CTGAAG 3 cut(s) 327, 693, 1029
EcoRII CCWGG 3 cut(s) 839, 886, 1178
FaeI CATG 4 cut(s) 294, 399, 660, 765
FalI AAGNNNNNCTT 6 cut(s) 108, 140, 474, 506, 1215, 1247
FaqI GGGAC 1 cut(s) 1250
FatI CATG 4 cut(s) 290, 395, 656, 761
FblI GTMKAC 1 cut(s) 996
Fnu4HI GCNGC 2 cut(s) 837, 914
FokI GGATG 4 cut(s) 195, 401, 561, 767
Fsp4HI GCNGC 2 cut(s) 837, 914
FspBI CTAG 1 cut(s) 30
GluI GCNGC 2 cut(s) 837, 914
HaeIII GGCC 2 cut(s) 1074, 1089
HapII CCGG 1 cut(s) 365
Hin1II CATG 4 cut(s) 294, 399, 660, 765
HinfI GANTC 2 cut(s) 883, 1160
HpaII CCGG 1 cut(s) 365
HphI GGTGA 1 cut(s) 32
Hpy166II GTNNAC 1 cut(s) 997
Hpy188I TCNGA 7 cut(s) 276, 376, 406, 642, 742, 772, 1159
Hpy188III TCNNGA 4 cut(s) 313, 679, 1139, 1220
Hpy8I GTNNAC 1 cut(s) 997
HpyAV CCTTC 9 cut(s) 149, 259, 515, 625, 1053, 1145, 1164, 1223, 1255
HpyCH4III ACNGT 2 cut(s) 310, 676
HpyCH4IV ACGT 1 cut(s) 999
HpyCH4V TGCA 4 cut(s) 260, 626, 851, 913
HpyF10VI GCNNNNNNNGC 2 cut(s) 833, 913
HpyF3I CTNAG 6 cut(s) 375, 403, 769, 828, 1140, 1232
HpySE526I ACGT 1 cut(s) 999
Hsp92II CATG 4 cut(s) 294, 399, 660, 765
Kzo9I GATC 6 cut(s) 70, 74, 406, 439, 772, 864
LmnI GCTCC 6 cut(s) 110, 158, 476, 524, 935, 937
Lsp1109I GCAGC 2 cut(s) 823, 925
LweI GCATC 2 cut(s) 215, 581
MaeI CTAG 1 cut(s) 30
MaeII ACGT 1 cut(s) 999
MaeIII GTNAC 1 cut(s) 367
MalI GATC 6 cut(s) 72, 76, 408, 441, 774, 866
MbiI CCGCTC 2 cut(s) 811, 907
MboI GATC 6 cut(s) 70, 74, 406, 439, 772, 864
MboII GAAGA 7 cut(s) 164, 324, 333, 530, 690, 699, 1235
MflI RGATCY 2 cut(s) 406, 772
MhlI GDGCHC 2 cut(s) 93, 459
MlsI TGGCCA 1 cut(s) 1074
MluCI AATT 8 cut(s) 174, 299, 540, 665, 819, 982, 1203, 1207
MluNI TGGCCA 1 cut(s) 1074
MlyI GAGTC 1 cut(s) 1154
MnlI CCTC 6 cut(s) 823, 826, 1024, 1227, 1262, 1280
Mox20I TGGCCA 1 cut(s) 1074
MroXI GAANNNNTTC 3 cut(s) 123, 489, 1265
MscI TGGCCA 1 cut(s) 1074
MseI TTAA 4 cut(s) 326, 692, 924, 1206
Msp20I TGGCCA 1 cut(s) 1074
MspI CCGG 1 cut(s) 365
MspR9I CCNGG 3 cut(s) 841, 888, 1180
Mva1269I GAATGC 1 cut(s) 1031
MvaI CCWGG 3 cut(s) 841, 888, 1180
MwoI GCNNNNNNNGC 2 cut(s) 833, 913
NdeII GATC 6 cut(s) 70, 74, 406, 439, 772, 864
NlaIII CATG 4 cut(s) 294, 399, 660, 765
NlaIV GGNNCC 2 cut(s) 931, 1090
NspV TTCGAA 1 cut(s) 1035
PctI GAATGC 1 cut(s) 1031
PdmI GAANNNNTTC 3 cut(s) 123, 489, 1265
PfeI GAWTC 1 cut(s) 883
PinAI ACCGGT 1 cut(s) 364
PkrI GCNGC 2 cut(s) 838, 915
PleI GAGTC 1 cut(s) 1154
PpsI GAGTC 1 cut(s) 1154
PshBI ATTAAT 1 cut(s) 1206
Psp6I CCWGG 3 cut(s) 839, 886, 1178
PspGI CCWGG 3 cut(s) 839, 886, 1178
PspN4I GGNNCC 2 cut(s) 931, 1090
PspPI GGNCC 2 cut(s) 902, 1088
PsrI GAACNNNNNNTAC 2 cut(s) 16, 48
PstI CTGCAG 2 cut(s) 262, 628
PstNI CAGNNNCTG 2 cut(s) 230, 596
PsuI RGATCY 2 cut(s) 406, 772
RsaI GTAC 3 cut(s) 216, 582, 767
RsaNI GTAC 3 cut(s) 215, 581, 766
SaqAI TTAA 4 cut(s) 326, 692, 924, 1206
SatI GCNGC 2 cut(s) 837, 914
Sau3AI GATC 6 cut(s) 70, 74, 406, 439, 772, 864
Sau96I GGNCC 2 cut(s) 902, 1088
SchI GAGTC 1 cut(s) 1154
ScrFI CCNGG 3 cut(s) 841, 888, 1180
SduI GDGCHC 2 cut(s) 93, 459
SfaNI GCATC 2 cut(s) 215, 581
SfcI CTRYAG 3 cut(s) 258, 624, 1100
SfuI TTCGAA 1 cut(s) 1035
SinI GGWCC 1 cut(s) 902
SmlI CTYRAG 1 cut(s) 1253
SmoI CTYRAG 1 cut(s) 1253
Sse9I AATT 8 cut(s) 174, 299, 540, 665, 819, 982, 1203, 1207
SsiI CCGC 2 cut(s) 809, 905
SspMI CTAG 1 cut(s) 30
StyD4I CCNGG 3 cut(s) 839, 886, 1178
TaaI ACNGT 2 cut(s) 310, 676
TaiI ACGT 1 cut(s) 1002
TaqI TCGA 6 cut(s) 73, 165, 531, 867, 878, 1035
TasI AATT 8 cut(s) 174, 299, 540, 665, 819, 982, 1203, 1207
TatI WGTACW 2 cut(s) 214, 580
TfiI GAWTC 1 cut(s) 883
Tru1I TTAA 4 cut(s) 326, 692, 924, 1206
Tru9I TTAA 4 cut(s) 326, 692, 924, 1206
TscAI CASTG 6 cut(s) 235, 267, 313, 601, 633, 679
TseI GCWGC 2 cut(s) 836, 913
TspDTI ATGAA 7 cut(s) 116, 188, 405, 482, 554, 771, 896
TspGWI ACGGA 1 cut(s) 922
TspRI CASTG 6 cut(s) 235, 267, 313, 601, 633, 679
VpaK11BI GGWCC 1 cut(s) 902
VspI ATTAAT 1 cut(s) 1206
XapI RAATTY 3 cut(s) 299, 665, 819
XcmI CCANNNNNNNNNTGG 1 cut(s) 1082
XmiI GTMKAC 1 cut(s) 996
XmnI GAANNNNTTC 3 cut(s) 123, 489, 1265
XspI CTAG 1 cut(s) 30
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.