pycom02g25700

TMV resistance protein N-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
N/A
Physical Location & Seq
Reverse (-)
23619485 .. 23619955
471 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 405 bp
ATGGAATTGACGAACAACCTCTACTGCACATTGAAAGACCACAGATTCAACGTCTTTATCGACGAGAACGAATTGACCAGAGGCGAAATTATCACACAAGCACTGAAGCAAGCAGTCAAAGACTCTAGGCTTGCTGCTGTCATCTTCTCAAGCGGGTATGCCGAGTCGAGATGGTGTCTTGATGAGCTGGTCGAGATCTTGGCCTGCAGAAGAATAATGGGGCAAATGGTTTTGCCAATTTTCTATGACATCGATCCCTCCGATGTCAGGCATCAGAGGGGTGCTTTTGAGGAAGCATTCCGGGAACATCAAAAGCGCTCCCCTGAAGTGGTGCAGACCTGGAAAGATGCTCTTAATGAAGCTGCAGAGTTGGGCGGCGAGGATTTTAGTGCGACTGATGGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NB-ARC TIR

Protein Analysis

135

Amino Acids

15.34

Weight (kDa)

4.77

Isoelectric Point (pI)

55.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 2 - 130 4.2e-40 TIR domain
TIR_2 PF13676 5 - 86 3.3e-12 TIR domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 153, 375
AclWI GGATC 1 cut(s) 248
AcuI CTGAAG 2 cut(s) 125, 345
AfeI AGCGCT 1 cut(s) 317
AfiI CCNNNNNNNGG 2 cut(s) 267, 328
AgsI TTSAA 2 cut(s) 34, 49
AjnI CCWGG 1 cut(s) 338
AluBI AGCT 2 cut(s) 187, 362
AluI AGCT 2 cut(s) 187, 362
AlwI GGATC 1 cut(s) 248
Aor51HI AGCGCT 1 cut(s) 317
AoxI GGCC 1 cut(s) 201
ApeKI GCWGC 2 cut(s) 134, 362
AspLEI GCGC 1 cut(s) 318
AsuC2I CCSGG 1 cut(s) 302
BbvI GCAGC 2 cut(s) 121, 349
BccI CCATC 2 cut(s) 165, 392
BciT130I CCWGG 1 cut(s) 340
BcnI CCSGG 1 cut(s) 302
BfaI CTAG 1 cut(s) 126
BfmI CTRYAG 2 cut(s) 205, 363
BfoI RGCGCY 1 cut(s) 319
BglII AGATCT 1 cut(s) 195
BisI GCNGC 3 cut(s) 135, 363, 376
BlsI GCNGC 3 cut(s) 136, 364, 377
Bme1390I CCNGG 2 cut(s) 302, 340
BmrFI CCNGG 2 cut(s) 302, 340
BmsI GCATC 2 cut(s) 280, 337
BpuEI CTTGAG 1 cut(s) 133
BpuMI CCSGG 1 cut(s) 302
Bsa29I ATCGAT 1 cut(s) 252
Bsc4I CCNNNNNNNGG 2 cut(s) 267, 328
BseBI CCWGG 1 cut(s) 340
BseCI ATCGAT 1 cut(s) 252
BseLI CCNNNNNNNGG 2 cut(s) 267, 328
BseXI GCAGC 2 cut(s) 121, 349
BsgI GTGCAG 2 cut(s) 10, 353
BshFI GGCC 1 cut(s) 203
BshVI ATCGAT 1 cut(s) 252
BsiSI CCGG 1 cut(s) 301
BslI CCNNNNNNNGG 2 cut(s) 267, 328
BsmI GAATGC 1 cut(s) 296
BsnI GGCC 1 cut(s) 203
Bsp143I GATC 2 cut(s) 195, 253
BspACI CCGC 2 cut(s) 153, 375
BspANI GGCC 1 cut(s) 203
BspDI ATCGAT 1 cut(s) 252
BspMAI CTGCAG 2 cut(s) 209, 367
BspPI GGATC 1 cut(s) 248
BssMI GATC 2 cut(s) 195, 253
Bst2UI CCWGG 1 cut(s) 340
BstC8I GCNNGC 3 cut(s) 111, 132, 205
BstH2I RGCGCY 1 cut(s) 319
BstHHI GCGC 1 cut(s) 318
BstKTI GATC 2 cut(s) 198, 256
BstMBI GATC 2 cut(s) 195, 253
BstNI CCWGG 1 cut(s) 340
BstSCI CCNGG 2 cut(s) 300, 338
BstSFI CTRYAG 2 cut(s) 205, 363
BstV1I GCAGC 2 cut(s) 121, 349
BstX2I RGATCY 1 cut(s) 195
BstYI RGATCY 1 cut(s) 195
Bsu15I ATCGAT 1 cut(s) 252
BsuRI GGCC 1 cut(s) 203
BsuTUI ATCGAT 1 cut(s) 252
BtsIMutI CAGTG 1 cut(s) 101
Cac8I GCNNGC 3 cut(s) 111, 132, 205
CfoI GCGC 1 cut(s) 318
ClaI ATCGAT 1 cut(s) 252
CviJI RGCY 4 cut(s) 130, 187, 203, 362
CviKI_1 RGCY 4 cut(s) 130, 187, 203, 362
DpnI GATC 2 cut(s) 197, 255
DpnII GATC 2 cut(s) 195, 253
Eco47III AGCGCT 1 cut(s) 317
Eco57I CTGAAG 2 cut(s) 125, 345
EcoRII CCWGG 1 cut(s) 338
FaiI YATR 2 cut(s) 159, 246
FalI AAGNNNNNCTT 2 cut(s) 336, 368
FauI CCCGC 1 cut(s) 146
Fnu4HI GCNGC 3 cut(s) 135, 363, 376
Fsp4HI GCNGC 3 cut(s) 135, 363, 376
FspBI CTAG 1 cut(s) 126
GlaI GCGC 1 cut(s) 317
GluI GCNGC 3 cut(s) 135, 363, 376
HaeII RGCGCY 1 cut(s) 319
HaeIII GGCC 1 cut(s) 203
HapII CCGG 1 cut(s) 301
HhaI GCGC 1 cut(s) 318
Hin6I GCGC 1 cut(s) 316
HinP1I GCGC 1 cut(s) 316
HinfI GANTC 3 cut(s) 45, 122, 164
HpaII CCGG 1 cut(s) 301
Hpy188I TCNGA 2 cut(s) 262, 276
Hpy188III TCNNGA 3 cut(s) 168, 179, 193
Hpy99I CGWCG 1 cut(s) 65
HpyCH4IV ACGT 1 cut(s) 51
HpyCH4V TGCA 4 cut(s) 27, 207, 334, 365
HpySE526I ACGT 1 cut(s) 51
HspAI GCGC 1 cut(s) 316
Kzo9I GATC 2 cut(s) 195, 253
LmnI GCTCC 1 cut(s) 323
LpnPI CCDG 8 cut(s) 91, 173, 217, 253, 314, 325, 336, 352
Lsp1109I GCAGC 2 cut(s) 121, 349
LweI GCATC 2 cut(s) 280, 337
MaeI CTAG 1 cut(s) 126
MaeII ACGT 1 cut(s) 51
MalI GATC 2 cut(s) 197, 255
MboI GATC 2 cut(s) 195, 253
MboII GAAGA 2 cut(s) 136, 222
MflI RGATCY 1 cut(s) 195
MluCI AATT 4 cut(s) 5, 71, 87, 237
MlyI GAGTC 2 cut(s) 116, 173
MnlI CCTC 6 cut(s) 29, 74, 268, 270, 283, 373
MseI TTAA 1 cut(s) 354
MspI CCGG 1 cut(s) 301
MspR9I CCNGG 2 cut(s) 302, 340
Mva1269I GAATGC 1 cut(s) 296
MvaI CCWGG 1 cut(s) 340
NciI CCSGG 1 cut(s) 302
NdeII GATC 2 cut(s) 195, 253
NmeAIII GCCGAG 1 cut(s) 187
PcsI WCGNNNNNNNCGW 3 cut(s) 57, 66, 258
PctI GAATGC 1 cut(s) 296
PfeI GAWTC 1 cut(s) 45
PfoI TCCNGGA 1 cut(s) 300
PkrI GCNGC 3 cut(s) 136, 364, 377
PleI GAGTC 2 cut(s) 116, 172
PpsI GAGTC 2 cut(s) 116, 172
Psp6I CCWGG 1 cut(s) 338
PspGI CCWGG 1 cut(s) 338
PsrI GAACNNNNNNTAC 1 cut(s) 37
PstI CTGCAG 2 cut(s) 209, 367
PsuI RGATCY 1 cut(s) 195
SaqAI TTAA 1 cut(s) 354
SatI GCNGC 3 cut(s) 135, 363, 376
Sau3AI GATC 2 cut(s) 195, 253
SchI GAGTC 2 cut(s) 116, 173
ScrFI CCNGG 2 cut(s) 302, 340
SetI ASST 5 cut(s) 21, 54, 189, 341, 364
SfaNI GCATC 2 cut(s) 280, 337
SfcI CTRYAG 2 cut(s) 205, 363
SmlI CTYRAG 1 cut(s) 148
SmoI CTYRAG 1 cut(s) 148
Sse9I AATT 4 cut(s) 5, 71, 87, 237
SsiI CCGC 2 cut(s) 153, 375
SspMI CTAG 1 cut(s) 126
StyD4I CCNGG 2 cut(s) 300, 338
TaiI ACGT 1 cut(s) 54
TaqI TCGA 4 cut(s) 60, 167, 192, 252
TasI AATT 4 cut(s) 5, 71, 87, 237
TauI GCSGC 1 cut(s) 378
TfiI GAWTC 1 cut(s) 45
Tru1I TTAA 1 cut(s) 354
Tru9I TTAA 1 cut(s) 354
TscAI CASTG 1 cut(s) 108
TseI GCWGC 2 cut(s) 134, 362
TspDTI ATGAA 1 cut(s) 372
TspRI CASTG 1 cut(s) 108
XspI CTAG 1 cut(s) 126
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.