Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr2

Orthogroup ID

N/A

Physical Location & Seq

Genomic Coordinates Reverse (-)

24553339 .. 24557040

Gene Structure 3702 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 651 bp

ATGACCCAGTTTACCACTGGTGTCATGGCCCTCCAGGTAGAGAGTGAATTCCAGAAGGCATATGAAAAGGGGATACACAAATCAAAGTACTGGGAGCCAACTTTTGAGGATTCACTTAGCTTGATTGCACAAGTGCCAGTAGTAGCTGCCTATATTTATCGAAGAATTTTCAAGGATGGAAAAGTAAAACAGATTGATGATTCTTTGGATTATGGTGCAAATTTTTCACACATGCTGGGTTTTGACGATCCCAAGGTGCATGAGCTCATGAGGCTTTATGTCACCATCCATAGGGATCATGAGGGTGGGAATGTCAGTGCTCACACGGGACACTTGGTTGCTAGTGCACTTTCAGATCCGTATCTTTCATTTGCAGCTGCTTTAAATGGCTTGGCTGGCCCACTCCATGGTTTGGCTAATCAGGAAGTATTGCTTTGGATAAAATCTGTGGTAGATGAAGTTGGAGAGAATGTAACTACCGAGCAGTTGAAAGATTATGTCTGGCAAACGTTAAAAAGTGGGAAGGTCGTTCCTGGTTTTGGACATGGGGTCTTGCGTAAAACAGACCCACGATACACATGTCAAAGGGAGTTTGCAATGAAACACATGCCTGATCGATCCGCTGTTTCAGTTGGTCTCCAAGCTTTATGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000166 GO:0003674 GO:0003824 GO:0004108 GO:0005488 GO:0005524 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0005739 GO:0005759 GO:0006082 GO:0006091 GO:0006099 GO:0006101 GO:0008144 GO:0008150 GO:0008152 GO:0008270 GO:0009060 GO:0009507 GO:0009536 GO:0009987 GO:0010035 GO:0010038 GO:0015980 GO:0016740 GO:0016746 GO:0016999 GO:0017076 GO:0017144 GO:0019752 GO:0030312 GO:0030554 GO:0031974 GO:0032553 GO:0032555 GO:0032559 GO:0035639 GO:0036094 GO:0036440 GO:0042221 GO:0043167 GO:0043168 GO:0043169 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043233 GO:0043436 GO:0044237 GO:0044238 GO:0044281 GO:0044422 GO:0044424 GO:0044429 GO:0044444 GO:0044446 GO:0044464 GO:0045333 GO:0046686 GO:0046872 GO:0046912 GO:0046914 GO:0050896 GO:0055114 GO:0070013 GO:0071704 GO:0071944 GO:0072350 GO:0097159 GO:0097367 GO:1901265 GO:1901363

KEGG Pathways

Metabolic & Signaling

ko00020 ko00630 ko01100 ko01110 ko01120 ko01130 ko01200 ko01210 ko01230 map00020 map00630 map01100 map01110 map01120 map01130 map01200 map01210 map01230

Pfam Domains

Protein Families

Citrate_synt

Protein Analysis

217

Amino Acids

24.11

Weight (kDa)

6.35

Isoelectric Point (pI)

19.58

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Citrate_synt	PF00285	1 - 204	6.2e-55	Citrate synthase, C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	2 cut(s)	407, 412
AciI	CCGC	1 cut(s)	621
AclI	AACGTT	1 cut(s)	509
AclWI	GGATC	4 cut(s)	242, 303, 350, 612
AcsI	RAATTY	3 cut(s)	47, 165, 220
AfaI	GTAC	1 cut(s)	89
AfiI	CCNNNNNNNGG	4 cut(s)	291, 407, 412, 539
AflIII	ACRYGT	1 cut(s)	578
AgsI	TTSAA	2 cut(s)	172, 490
AhdI	GACNNNNNGTC	1 cut(s)	548
AjnI	CCWGG	2 cut(s)	33, 532
AluBI	AGCT	5 cut(s)	120, 146, 265, 377, 644
AluI	AGCT	5 cut(s)	120, 146, 265, 377, 644
Alw21I	GWGCWC	3 cut(s)	267, 322, 349
Alw26I	GTCTC	1 cut(s)	641
Alw44I	GTGCAC	1 cut(s)	345
AlwI	GGATC	4 cut(s)	242, 303, 350, 612
AoxI	GGCC	2 cut(s)	27, 397
ApaLI	GTGCAC	1 cut(s)	345
ApeKI	GCWGC	3 cut(s)	146, 374, 377
ApoI	RAATTY	3 cut(s)	47, 165, 220
AspS9I	GGNCC	2 cut(s)	28, 398
AsuHPI	GGTGA	1 cut(s)	274
BaeGI	GKGCMC	1 cut(s)	349
BaeI	ACNNNNGTAYC	2 cut(s)	565, 598
BanII	GRGCYC	1 cut(s)	267
Bbv12I	GWGCWC	3 cut(s)	267, 322, 349
BbvI	GCAGC	3 cut(s)	133, 364, 386
BccI	CCATC	2 cut(s)	170, 293
BciT130I	CCWGG	2 cut(s)	35, 534
BciVI	GTATCC	1 cut(s)	66
BcoDI	GTCTC	1 cut(s)	641
BfaI	CTAG	1 cut(s)	342
BfuI	GTATCC	1 cut(s)	66
BisI	GCNGC	3 cut(s)	147, 375, 378
BlsI	GCNGC	3 cut(s)	148, 376, 379
BmcAI	AGTACT	1 cut(s)	89
Bme1390I	CCNGG	2 cut(s)	35, 534
BmeRI	GACNNNNNGTC	1 cut(s)	548
BmgT120I	GGNCC	2 cut(s)	28, 398
BmiI	GGNNCC	1 cut(s)	96
BmrFI	CCNGG	2 cut(s)	35, 534
BmrI	ACTGGG	1 cut(s)	100
BmuI	ACTGGG	1 cut(s)	100
BpmI	CTGGAG	1 cut(s)	17
Bsa29I	ATCGAT	1 cut(s)	616
BsaBI	GATNNNNATC	1 cut(s)	360
BsaI	GGTCTC	1 cut(s)	641
BsaJI	CCNNGG	2 cut(s)	252, 406
BsaXI	ACNNNNNCTCC	2 cut(s)	456, 486
Bsc4I	CCNNNNNNNGG	4 cut(s)	291, 407, 412, 539
Bse1I	ACTGG	4 cut(s)	7, 22, 95, 137
Bse3DI	GCAATG	1 cut(s)	603
Bse8I	GATNNNNATC	1 cut(s)	360
BseBI	CCWGG	2 cut(s)	35, 534
BseCI	ATCGAT	1 cut(s)	616
BseDI	CCNNGG	2 cut(s)	252, 406
BseGI	GGATG	2 cut(s)	181, 285
BseJI	GATNNNNATC	1 cut(s)	360
BseLI	CCNNNNNNNGG	4 cut(s)	291, 407, 412, 539
BseMI	GCAATG	1 cut(s)	603
BseNI	ACTGG	4 cut(s)	7, 22, 95, 137
BseSI	GKGCMC	1 cut(s)	349
BseXI	GCAGC	3 cut(s)	133, 364, 386
BseYI	CCCAGC	1 cut(s)	235
BshFI	GGCC	2 cut(s)	29, 399
BshVI	ATCGAT	1 cut(s)	616
BsiHKAI	GWGCWC	3 cut(s)	267, 322, 349
BslFI	GGGAC	1 cut(s)	342
BslI	CCNNNNNNNGG	4 cut(s)	291, 407, 412, 539
BsmAI	GTCTC	1 cut(s)	641
BsmFI	GGGAC	1 cut(s)	342
BsnI	GGCC	2 cut(s)	29, 399
Bso31I	GGTCTC	1 cut(s)	641
Bsp1286I	GDGCHC	3 cut(s)	267, 322, 349
Bsp143I	GATC	5 cut(s)	247, 295, 355, 613, 617
Bsp19I	CCATGG	1 cut(s)	406
BspACI	CCGC	1 cut(s)	621
BspANI	GGCC	2 cut(s)	29, 399
BspDI	ATCGAT	1 cut(s)	616
BspHI	TCATGA	2 cut(s)	267, 298
BspLI	GGNNCC	1 cut(s)	96
BspPI	GGATC	4 cut(s)	242, 303, 350, 612
BspTNI	GGTCTC	1 cut(s)	641
BsrDI	GCAATG	1 cut(s)	603
BsrI	ACTGG	4 cut(s)	7, 22, 95, 137
BssECI	CCNNGG	2 cut(s)	252, 406
BssMI	GATC	5 cut(s)	247, 295, 355, 613, 617
BssT1I	CCWWGG	2 cut(s)	252, 406
Bst2UI	CCWGG	2 cut(s)	35, 534
BstC8I	GCNNGC	1 cut(s)	397
BstDEI	CTNAG	1 cut(s)	116
BstDSI	CCRYGG	1 cut(s)	406
BstF5I	GGATG	2 cut(s)	181, 285
BstKTI	GATC	5 cut(s)	250, 298, 358, 616, 620
BstMAI	GTCTC	1 cut(s)	641
BstMBI	GATC	5 cut(s)	247, 295, 355, 613, 617
BstMWI	GCNNNNNNNGC	2 cut(s)	271, 396
BstNI	CCWGG	2 cut(s)	35, 534
BstNSI	RCATGY	3 cut(s)	235, 582, 610
BstSCI	CCNGG	2 cut(s)	33, 532
BstSLI	GKGCMC	1 cut(s)	349
BstV1I	GCAGC	3 cut(s)	133, 364, 386
BstX2I	RGATCY	1 cut(s)	355
BstYI	RGATCY	1 cut(s)	355
Bsu15I	ATCGAT	1 cut(s)	616
BsuI	GTATCC	1 cut(s)	66
BsuRI	GGCC	2 cut(s)	29, 399
BsuTUI	ATCGAT	1 cut(s)	616
BtgI	CCRYGG	1 cut(s)	406
BtsCI	GGATG	2 cut(s)	181, 285
BtsIMutI	CAGTG	2 cut(s)	15, 322
Cac8I	GCNNGC	1 cut(s)	397
CciI	TCATGA	2 cut(s)	267, 298
Cfr13I	GGNCC	2 cut(s)	28, 398
ClaI	ATCGAT	1 cut(s)	616
Csp6I	GTAC	1 cut(s)	88
CviAII	CATG	9 cut(s)	25, 232, 260, 268, 299, 407, 545, 579, 607
CviQI	GTAC	1 cut(s)	88
DdeI	CTNAG	1 cut(s)	116
DpnI	GATC	5 cut(s)	249, 297, 357, 615, 619
DpnII	GATC	5 cut(s)	247, 295, 355, 613, 617
DraI	TTTAAA	1 cut(s)	384
DriI	GACNNNNNGTC	1 cut(s)	548
Eam1105I	GACNNNNNGTC	1 cut(s)	548
Ecl136II	GAGCTC	1 cut(s)	265
Eco130I	CCWWGG	2 cut(s)	252, 406
Eco24I	GRGCYC	1 cut(s)	267
Eco31I	GGTCTC	1 cut(s)	641
Eco53kI	GAGCTC	1 cut(s)	265
EcoICRI	GAGCTC	1 cut(s)	265
EcoRI	GAATTC	1 cut(s)	47
EcoRII	CCWGG	2 cut(s)	33, 532
EcoT14I	CCWWGG	2 cut(s)	252, 406
EcoT38I	GRGCYC	1 cut(s)	267
ErhI	CCWWGG	2 cut(s)	252, 406
FaeI	CATG	9 cut(s)	28, 235, 263, 271, 302, 410, 548, 582, 610
FalI	AAGNNNNNCTT	2 cut(s)	417, 449
FaqI	GGGAC	1 cut(s)	342
FatI	CATG	9 cut(s)	24, 231, 259, 267, 298, 406, 544, 578, 606
FauNDI	CATATG	1 cut(s)	61
Fnu4HI	GCNGC	3 cut(s)	147, 375, 378
FokI	GGATG	2 cut(s)	188, 272
FriOI	GRGCYC	1 cut(s)	267
Fsp4HI	GCNGC	3 cut(s)	147, 375, 378
FspBI	CTAG	1 cut(s)	342
GluI	GCNGC	3 cut(s)	147, 375, 378
GsaI	CCCAGC	1 cut(s)	239
GsuI	CTGGAG	1 cut(s)	17
HaeIII	GGCC	2 cut(s)	29, 399
Hin1II	CATG	9 cut(s)	28, 235, 263, 271, 302, 410, 548, 582, 610
HindIII	AAGCTT	1 cut(s)	642
HinfI	GANTC	2 cut(s)	110, 200
HphI	GGTGA	1 cut(s)	274
Hpy166II	GTNNAC	2 cut(s)	12, 347
Hpy188I	TCNGA	1 cut(s)	355
Hpy188III	TCNNGA	4 cut(s)	52, 268, 299, 422
Hpy8I	GTNNAC	2 cut(s)	12, 347
HpyAV	CCTTC	2 cut(s)	49, 517
HpyCH4IV	ACGT	1 cut(s)	509
HpyCH4V	TGCA	6 cut(s)	128, 218, 259, 347, 374, 596
HpyF10VI	GCNNNNNNNGC	2 cut(s)	271, 396
HpyF3I	CTNAG	1 cut(s)	116
HpySE526I	ACGT	1 cut(s)	509
Hsp92II	CATG	9 cut(s)	28, 235, 263, 271, 302, 410, 548, 582, 610
Kzo9I	GATC	5 cut(s)	247, 295, 355, 613, 617
LmnI	GCTCC	1 cut(s)	94
Lsp1109I	GCAGC	3 cut(s)	133, 364, 386
MaeI	CTAG	1 cut(s)	342
MaeII	ACGT	1 cut(s)	509
MaeIII	GTNAC	2 cut(s)	280, 472
MalI	GATC	5 cut(s)	249, 297, 357, 615, 619
MboI	GATC	5 cut(s)	247, 295, 355, 613, 617
MboII	GAAGA	1 cut(s)	174
MflI	RGATCY	1 cut(s)	355
MhlI	GDGCHC	3 cut(s)	267, 322, 349
MluCI	AATT	3 cut(s)	47, 165, 220
MmeI	TCCRAC	1 cut(s)	442
MnlI	CCTC	4 cut(s)	41, 100, 264, 295
MseI	TTAA	2 cut(s)	383, 512
MslI	CAYNNNNRTG	1 cut(s)	303
MspA1I	CMGCKG	2 cut(s)	377, 623
MspR9I	CCNGG	2 cut(s)	35, 534
MvaI	CCWGG	2 cut(s)	35, 534
MwoI	GCNNNNNNNGC	2 cut(s)	271, 396
NcoI	CCATGG	1 cut(s)	406
NdeI	CATATG	1 cut(s)	61
NdeII	GATC	5 cut(s)	247, 295, 355, 613, 617
NlaIII	CATG	9 cut(s)	28, 235, 263, 271, 302, 410, 548, 582, 610
NlaIV	GGNNCC	1 cut(s)	96
NmuCI	GTSAC	1 cut(s)	280
NspI	RCATGY	3 cut(s)	235, 582, 610
PagI	TCATGA	2 cut(s)	267, 298
PciI	ACATGT	1 cut(s)	578
PfeI	GAWTC	2 cut(s)	110, 200
PflMI	CCANNNNNTGG	2 cut(s)	407, 412
PkrI	GCNGC	3 cut(s)	148, 376, 379
PscI	ACATGT	1 cut(s)	578
Psp124BI	GAGCTC	1 cut(s)	267
Psp1406I	AACGTT	1 cut(s)	509
Psp6I	CCWGG	2 cut(s)	33, 532
PspFI	CCCAGC	1 cut(s)	235
PspGI	CCWGG	2 cut(s)	33, 532
PspN4I	GGNNCC	1 cut(s)	96
PspPI	GGNCC	2 cut(s)	28, 398
PsuI	RGATCY	1 cut(s)	355
PvuII	CAGCTG	1 cut(s)	377
RsaI	GTAC	1 cut(s)	89
RsaNI	GTAC	1 cut(s)	88
RseI	CAYNNNNRTG	1 cut(s)	303
SacI	GAGCTC	1 cut(s)	267
SaqAI	TTAA	2 cut(s)	383, 512
SatI	GCNGC	3 cut(s)	147, 375, 378
Sau3AI	GATC	5 cut(s)	247, 295, 355, 613, 617
Sau96I	GGNCC	2 cut(s)	28, 398
ScaI	AGTACT	1 cut(s)	89
ScrFI	CCNGG	2 cut(s)	35, 534
SduI	GDGCHC	3 cut(s)	267, 322, 349
SetI	ASST	9 cut(s)	39, 122, 148, 258, 267, 379, 512, 528, 646
SmiMI	CAYNNNNRTG	1 cut(s)	303
Sse9I	AATT	3 cut(s)	47, 165, 220
SsiI	CCGC	1 cut(s)	621
SspMI	CTAG	1 cut(s)	342
SstI	GAGCTC	1 cut(s)	267
StyD4I	CCNGG	2 cut(s)	33, 532
StyI	CCWWGG	2 cut(s)	252, 406
TaiI	ACGT	1 cut(s)	512
TaqI	TCGA	2 cut(s)	160, 616
TasI	AATT	3 cut(s)	47, 165, 220
TatI	WGTACW	1 cut(s)	87
TfiI	GAWTC	2 cut(s)	110, 200
Tru1I	TTAA	2 cut(s)	383, 512
Tru9I	TTAA	2 cut(s)	383, 512
TscAI	CASTG	2 cut(s)	22, 322
TseFI	GTSAC	1 cut(s)	280
TseI	GCWGC	3 cut(s)	146, 374, 377
Tsp45I	GTSAC	1 cut(s)	280
TspDTI	ATGAA	4 cut(s)	78, 357, 471, 614
TspGWI	ACGGA	1 cut(s)	348
TspRI	CASTG	2 cut(s)	22, 322
Van91I	CCANNNNNTGG	2 cut(s)	407, 412
VneI	GTGCAC	1 cut(s)	345
XapI	RAATTY	3 cut(s)	47, 165, 220
XceI	RCATGY	3 cut(s)	235, 582, 610
XcmI	CCANNNNNNNNNTGG	2 cut(s)	14, 22
XspI	CTAG	1 cut(s)	342
ZrmI	AGTACT	1 cut(s)	89

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.