pycom03g02520

Pentatricopeptide repeat-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
N/A
Physical Location & Seq
Reverse (-)
1905297 .. 1906507
1211 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 609 bp
ATGGACGTTGATGATTTCAGGGGTTTGCCATGCATGGGCAAGCTGAGGAAGCTCTTAAGACTTTTCTCTAAAATGATTCAGAAAGGCGTGGATCTGAATGATGTATCCTTTGTCGGAGTGTTGCATGCTTGTAGTCACATTGGGTTTGTGGATCAGGGCTCCTTCAAGAGGCTCGCGATCGAGTTTATCACAAACATGCCTATCAAGCCCAATAGTGTCGTATGGGGAGCTCACCTTGGCGGATGCAAAGTTCACAAAAACATTGAACTGGCTGAAGAAGCAACAAAGCACCTTTCGGAATTGGATCCACTTAATGATGGCTACTATGTGGTTCTGTCAAACATTTATGCAGAAGCACAACGGTGGGAAGACACAGCGACGGTGAGAAAGATAATGAGGGATCGAGGGAGGGAAATTAGTGCTGGTTTTCGGGTTCATCAACACAAAACATGGAACACCAATTCGAATTATGATCTTCGTGTTTGCGAGGACTGTCATGCTGCTTTCAAACTTATATCAGCAATTGTTAGTACAGAGATCGTTGTGCGTGACCGTAACCGGTTCCATTGTTTCAAAGATGGCTTTTGTTCCTGCCGGGATTACTGGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DYW_deaminase PPR PPR_2

Protein Analysis

203

Amino Acids

23.18

Weight (kDa)

8.41

Isoelectric Point (pI)

36.51

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
E_motif PF20431 85 - 137 7.5e-16 E motif
DYW_deaminase PF14432 157 - 202 6.2e-19 DYW family of nucleic acid deaminases
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 176
AciI CCGC 1 cut(s) 240
AclWI GGATC 5 cut(s) 99, 159, 299, 312, 408
AcuI CTGAAG 1 cut(s) 294
AfaI GTAC 1 cut(s) 532
AfiI CCNNNNNNNGG 2 cut(s) 35, 168
AflII CTTAAG 1 cut(s) 55
AgeI ACCGGT 1 cut(s) 558
AgsI TTSAA 4 cut(s) 166, 266, 508, 574
AleI CACNNNNGTG 1 cut(s) 361
AloI GAACNNNNNNTCC 2 cut(s) 234, 266
AluBI AGCT 3 cut(s) 43, 52, 230
AluI AGCT 3 cut(s) 43, 52, 230
Alw21I GWGCWC 1 cut(s) 232
AlwI GGATC 5 cut(s) 99, 159, 299, 312, 408
ApeKI GCWGC 1 cut(s) 500
AsiGI ACCGGT 1 cut(s) 558
AsuC2I CCSGG 1 cut(s) 596
AsuHPI GGTGA 2 cut(s) 224, 394
AsuII TTCGAA 1 cut(s) 464
BamHI GGATCC 1 cut(s) 304
BanII GRGCYC 2 cut(s) 161, 232
BbsI GAAGAC 1 cut(s) 375
Bbv12I GWGCWC 1 cut(s) 232
BbvCI CCTCAGC 1 cut(s) 44
BbvI GCAGC 1 cut(s) 487
BccI CCATC 2 cut(s) 311, 572
BciVI GTATCC 1 cut(s) 115
BcnI CCSGG 1 cut(s) 596
BfrI CTTAAG 1 cut(s) 55
BfuI GTATCC 1 cut(s) 115
BisI GCNGC 1 cut(s) 501
BlsI GCNGC 1 cut(s) 502
Bme1390I CCNGG 1 cut(s) 596
BmiI GGNNCC 3 cut(s) 160, 306, 563
BmrFI CCNGG 1 cut(s) 596
BmsI GCATC 1 cut(s) 233
BpiI GAAGAC 1 cut(s) 375
Bpu10I CCTNAGC 1 cut(s) 44
Bpu14I TTCGAA 1 cut(s) 464
BpuMI CCSGG 1 cut(s) 596
BsaJI CCNNGG 1 cut(s) 235
BsaWI WCCGGW 1 cut(s) 558
Bsc4I CCNNNNNNNGG 2 cut(s) 35, 168
Bse118I RCCGGY 1 cut(s) 558
Bse1I ACTGG 2 cut(s) 273, 608
BseDI CCNNGG 1 cut(s) 235
BseGI GGATG 1 cut(s) 248
BseLI CCNNNNNNNGG 2 cut(s) 35, 168
BseMII CTCAG 1 cut(s) 35
BseNI ACTGG 2 cut(s) 273, 608
BseXI GCAGC 1 cut(s) 487
Bsh1236I CGCG 1 cut(s) 176
Bsh1285I CGRYCG 1 cut(s) 180
BshTI ACCGGT 1 cut(s) 558
BsiEI CGRYCG 1 cut(s) 180
BsiHKAI GWGCWC 1 cut(s) 232
BsiSI CCGG 2 cut(s) 559, 595
BslI CCNNNNNNNGG 2 cut(s) 35, 168
Bsp119I TTCGAA 1 cut(s) 464
Bsp1286I GDGCHC 2 cut(s) 161, 232
Bsp143I GATC 7 cut(s) 91, 151, 177, 304, 400, 472, 537
Bsp68I TCGCGA 1 cut(s) 176
BspACI CCGC 1 cut(s) 240
BspCNI CTCAG 1 cut(s) 36
BspFNI CGCG 1 cut(s) 176
BspLI GGNNCC 3 cut(s) 160, 306, 563
BspPI GGATC 5 cut(s) 99, 159, 299, 312, 408
BspT104I TTCGAA 1 cut(s) 464
BspTI CTTAAG 1 cut(s) 55
BsrFI RCCGGY 1 cut(s) 558
BsrI ACTGG 2 cut(s) 273, 608
BssAI RCCGGY 1 cut(s) 558
BssECI CCNNGG 1 cut(s) 235
BssMI GATC 7 cut(s) 91, 151, 177, 304, 400, 472, 537
BssT1I CCWWGG 1 cut(s) 235
Bst4CI ACNGT 4 cut(s) 363, 382, 494, 554
BstAFI CTTAAG 1 cut(s) 55
BstBI TTCGAA 1 cut(s) 464
BstC8I GCNNGC 3 cut(s) 41, 126, 174
BstDEI CTNAG 1 cut(s) 44
BstENI CCTNNNNNAGG 1 cut(s) 166
BstF5I GGATG 1 cut(s) 248
BstFNI CGCG 1 cut(s) 176
BstKTI GATC 7 cut(s) 94, 154, 180, 307, 403, 475, 540
BstMBI GATC 7 cut(s) 91, 151, 177, 304, 400, 472, 537
BstMCI CGRYCG 1 cut(s) 180
BstMWI GCNNNNNNNGC 3 cut(s) 49, 205, 278
BstNSI RCATGY 2 cut(s) 128, 199
BstSCI CCNGG 1 cut(s) 594
BstUI CGCG 1 cut(s) 176
BstV1I GCAGC 1 cut(s) 487
BstV2I GAAGAC 1 cut(s) 375
BstX2I RGATCY 2 cut(s) 91, 304
BstYI RGATCY 2 cut(s) 91, 304
BsuI GTATCC 1 cut(s) 115
BtsCI GGATG 1 cut(s) 248
BtuMI TCGCGA 1 cut(s) 176
Cac8I GCNNGC 3 cut(s) 41, 126, 174
Cfr10I RCCGGY 1 cut(s) 558
Csp6I GTAC 1 cut(s) 531
CspAI ACCGGT 1 cut(s) 558
CviAII CATG 6 cut(s) 30, 34, 125, 196, 450, 497
CviJI RGCY 9 cut(s) 43, 52, 159, 172, 208, 230, 272, 321, 582
CviKI_1 RGCY 9 cut(s) 43, 52, 159, 172, 208, 230, 272, 321, 582
CviQI GTAC 1 cut(s) 531
DdeI CTNAG 1 cut(s) 44
DpnI GATC 7 cut(s) 93, 153, 179, 306, 402, 474, 539
DpnII GATC 7 cut(s) 91, 151, 177, 304, 400, 472, 537
EciI GGCGGA 1 cut(s) 255
Ecl136II GAGCTC 1 cut(s) 230
Eco130I CCWWGG 1 cut(s) 235
Eco24I GRGCYC 2 cut(s) 161, 232
Eco53kI GAGCTC 1 cut(s) 230
Eco57I CTGAAG 1 cut(s) 294
EcoICRI GAGCTC 1 cut(s) 230
EcoNI CCTNNNNNAGG 1 cut(s) 166
EcoT14I CCWWGG 1 cut(s) 235
EcoT22I ATGCAT 1 cut(s) 35
EcoT38I GRGCYC 2 cut(s) 161, 232
ErhI CCWWGG 1 cut(s) 235
FaeI CATG 6 cut(s) 33, 37, 128, 199, 453, 500
FatI CATG 6 cut(s) 29, 33, 124, 195, 449, 496
Fnu4HI GCNGC 1 cut(s) 501
FokI GGATG 1 cut(s) 255
FriOI GRGCYC 2 cut(s) 161, 232
Fsp4HI GCNGC 1 cut(s) 501
GluI GCNGC 1 cut(s) 501
HapII CCGG 2 cut(s) 559, 595
Hin1II CATG 6 cut(s) 33, 37, 128, 199, 453, 500
HinfI GANTC 1 cut(s) 76
HpaII CCGG 2 cut(s) 559, 595
HphI GGTGA 2 cut(s) 224, 394
Hpy166II GTNNAC 1 cut(s) 253
Hpy188I TCNGA 4 cut(s) 81, 96, 116, 298
Hpy188III TCNNGA 2 cut(s) 166, 175
Hpy8I GTNNAC 1 cut(s) 253
Hpy99I CGWCG 1 cut(s) 382
HpyAV CCTTC 1 cut(s) 172
HpyCH4III ACNGT 4 cut(s) 363, 382, 494, 554
HpyCH4IV ACGT 1 cut(s) 6
HpyCH4V TGCA 4 cut(s) 33, 124, 246, 350
HpyF10VI GCNNNNNNNGC 3 cut(s) 49, 205, 278
HpyF3I CTNAG 1 cut(s) 44
HpySE526I ACGT 1 cut(s) 6
Hsp92II CATG 6 cut(s) 33, 37, 128, 199, 453, 500
Kzo9I GATC 7 cut(s) 91, 151, 177, 304, 400, 472, 537
LmnI GCTCC 2 cut(s) 164, 227
LpnPI CCDG 7 cut(s) 4, 140, 254, 408, 572, 589, 604
Lsp1109I GCAGC 1 cut(s) 487
LweI GCATC 1 cut(s) 233
MaeII ACGT 1 cut(s) 6
MaeIII GTNAC 3 cut(s) 134, 548, 554
MalI GATC 7 cut(s) 93, 153, 179, 306, 402, 474, 539
MboI GATC 7 cut(s) 91, 151, 177, 304, 400, 472, 537
MboII GAAGA 3 cut(s) 287, 380, 467
MfeI CAATTG 1 cut(s) 522
MflI RGATCY 2 cut(s) 91, 304
MhlI GDGCHC 2 cut(s) 161, 232
MluCI AATT 5 cut(s) 299, 414, 460, 466, 522
MmeI TCCRAC 1 cut(s) 94
MnlI CCTC 6 cut(s) 39, 162, 390, 398, 402, 481
Mph1103I ATGCAT 1 cut(s) 35
MseI TTAA 2 cut(s) 56, 312
MslI CAYNNNNRTG 2 cut(s) 194, 361
MspCI CTTAAG 1 cut(s) 55
MspI CCGG 2 cut(s) 559, 595
MspR9I CCNGG 1 cut(s) 596
MunI CAATTG 1 cut(s) 522
MvnI CGCG 1 cut(s) 176
MwoI GCNNNNNNNGC 3 cut(s) 49, 205, 278
NciI CCSGG 1 cut(s) 596
NdeII GATC 7 cut(s) 91, 151, 177, 304, 400, 472, 537
NlaIII CATG 6 cut(s) 33, 37, 128, 199, 453, 500
NlaIV GGNNCC 3 cut(s) 160, 306, 563
NmuCI GTSAC 2 cut(s) 134, 548
NruI TCGCGA 1 cut(s) 176
NsiI ATGCAT 1 cut(s) 35
NspI RCATGY 2 cut(s) 128, 199
NspV TTCGAA 1 cut(s) 464
OliI CACNNNNGTG 1 cut(s) 361
PaeI GCATGC 1 cut(s) 128
PfeI GAWTC 1 cut(s) 76
PinAI ACCGGT 1 cut(s) 558
PkrI GCNGC 1 cut(s) 502
Ple19I CGATCG 1 cut(s) 180
Psp124BI GAGCTC 1 cut(s) 232
PspN4I GGNNCC 3 cut(s) 160, 306, 563
PsuI RGATCY 2 cut(s) 91, 304
PvuI CGATCG 1 cut(s) 180
RruI TCGCGA 1 cut(s) 176
RsaI GTAC 1 cut(s) 532
RsaNI GTAC 1 cut(s) 531
RseI CAYNNNNRTG 2 cut(s) 194, 361
SacI GAGCTC 1 cut(s) 232
SaqAI TTAA 2 cut(s) 56, 312
SatI GCNGC 1 cut(s) 501
Sau3AI GATC 7 cut(s) 91, 151, 177, 304, 400, 472, 537
ScrFI CCNGG 1 cut(s) 596
SduI GDGCHC 2 cut(s) 161, 232
SetI ASST 6 cut(s) 9, 45, 54, 232, 237, 294
SfaNI GCATC 1 cut(s) 233
SfuI TTCGAA 1 cut(s) 464
SmiMI CAYNNNNRTG 2 cut(s) 194, 361
SmlI CTYRAG 1 cut(s) 55
SmoI CTYRAG 1 cut(s) 55
SphI GCATGC 1 cut(s) 128
Sse9I AATT 5 cut(s) 299, 414, 460, 466, 522
SsiI CCGC 1 cut(s) 240
SstI GAGCTC 1 cut(s) 232
StyD4I CCNGG 1 cut(s) 594
StyI CCWWGG 1 cut(s) 235
TaaI ACNGT 4 cut(s) 363, 382, 494, 554
TaiI ACGT 1 cut(s) 9
TaqI TCGA 3 cut(s) 180, 403, 464
TasI AATT 5 cut(s) 299, 414, 460, 466, 522
TatI WGTACW 1 cut(s) 530
TfiI GAWTC 1 cut(s) 76
Tru1I TTAA 2 cut(s) 56, 312
Tru9I TTAA 2 cut(s) 56, 312
TseFI GTSAC 2 cut(s) 134, 548
TseI GCWGC 1 cut(s) 500
Tsp45I GTSAC 2 cut(s) 134, 548
TspDTI ATGAA 1 cut(s) 425
Vha464I CTTAAG 1 cut(s) 55
XagI CCTNNNNNAGG 1 cut(s) 166
XceI RCATGY 2 cut(s) 128, 199
Zsp2I ATGCAT 1 cut(s) 35
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.