pycom03g02590

Coiled-coil domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
N/A
Physical Location & Seq
Reverse (-)
1980745 .. 1982155
1411 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 471 bp
ATGTTTATGCACTATATTGCTCAAGAAACGACGTCGAATCAAACTCTTTCCGGCGAAACGAACAGAACAAAGAACTCAAACAACTGCCACTCTACACTGAAAATCCAAAACTCGCAGAAAATCTCTTCAATTCCATCAATCGGCAATGGAAGATTCGCGGGAAATACTGCTGAAGTCGTTGGAGAGCTCCGGCGTATGTGCCCCGGACGACGTTCGTCTGTTAAGGACCTGACGCTGGCTGCCTTGGTCTCTATCTGCGGCCAGTCTCTCAACCTCACCGGCCAAACGACGCCGTTTCCCACTTCCCTCCCCGACTCCTCCGTCGCCGACCAGTTCAAGCTCTGTACGGACATTGCCTCCGGGATCAAGAGCCTCGGTTACCTCGCCGACCTGAGCTTCCATCAGAAACAGACTATATGTGTTGCTTACGGCAGTTCTTGTATCCATCTGAAGAGGGCTCGTACAAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF812

Protein Analysis

157

Amino Acids

16.83

Weight (kDa)

9.32

Isoelectric Point (pI)

41.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
CCDC22_N PF21674 70 - 135 3.1e-11 CCDC22 protein N-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 320
AatII GACGTC 1 cut(s) 35
AccII CGCG 1 cut(s) 158
AciI CCGC 2 cut(s) 158, 258
AclWI GGATC 1 cut(s) 371
AcoI YGGCCR 2 cut(s) 259, 280
AcuI CTGAAG 2 cut(s) 192, 470
AcyI GRCGYC 2 cut(s) 32, 290
AfaI GTAC 2 cut(s) 346, 463
AfiI CCNNNNNNNGG 2 cut(s) 140, 235
AgsI TTSAA 2 cut(s) 129, 337
AluBI AGCT 3 cut(s) 187, 340, 396
AluI AGCT 3 cut(s) 187, 340, 396
Alw21I GWGCWC 1 cut(s) 189
Alw26I GTCTC 2 cut(s) 253, 270
AlwI GGATC 1 cut(s) 371
AoxI GGCC 2 cut(s) 259, 280
ApeKI GCWGC 1 cut(s) 239
AspS9I GGNCC 1 cut(s) 226
AsuC2I CCSGG 2 cut(s) 204, 361
AsuHPI GGTGA 1 cut(s) 268
AvaII GGWCC 1 cut(s) 226
BaeGI GKGCMC 1 cut(s) 203
BanII GRGCYC 2 cut(s) 189, 460
Bbv12I GWGCWC 1 cut(s) 189
BbvI GCAGC 1 cut(s) 226
BccI CCATC 3 cut(s) 142, 408, 453
BceAI ACGGC 2 cut(s) 277, 445
BciVI GTATCC 1 cut(s) 452
BcnI CCSGG 2 cut(s) 204, 361
BcoDI GTCTC 2 cut(s) 253, 270
BfuI GTATCC 1 cut(s) 452
BisI GCNGC 2 cut(s) 240, 259
BlsI GCNGC 2 cut(s) 241, 260
Bme1390I CCNGG 2 cut(s) 204, 361
Bme18I GGWCC 1 cut(s) 226
BmgT120I GGNCC 1 cut(s) 226
BmrFI CCNGG 2 cut(s) 204, 361
BoxI GACNNNNGTC 1 cut(s) 214
Bpu10I CCTNAGC 1 cut(s) 392
BpuEI CTTGAG 1 cut(s) 6
BpuMI CCSGG 2 cut(s) 204, 361
BsaHI GRCGYC 2 cut(s) 32, 290
BsaI GGTCTC 1 cut(s) 253
BsaJI CCNNGG 3 cut(s) 202, 243, 373
BsaXI ACNNNNNCTCC 2 cut(s) 341, 371
Bsc4I CCNNNNNNNGG 2 cut(s) 140, 235
Bse118I RCCGGY 1 cut(s) 278
Bse1I ACTGG 2 cut(s) 262, 331
Bse3DI GCAATG 2 cut(s) 151, 351
BseDI CCNNGG 3 cut(s) 202, 243, 373
BseLI CCNNNNNNNGG 2 cut(s) 140, 235
BseMI GCAATG 2 cut(s) 151, 351
BseMII CTCAG 1 cut(s) 383
BseNI ACTGG 2 cut(s) 262, 331
BseRI GAGGAG 1 cut(s) 307
BseSI GKGCMC 1 cut(s) 203
BseXI GCAGC 1 cut(s) 226
Bsh1236I CGCG 1 cut(s) 158
BshFI GGCC 2 cut(s) 261, 282
BsiHKAI GWGCWC 1 cut(s) 189
BsiSI CCGG 5 cut(s) 51, 190, 204, 279, 360
BslI CCNNNNNNNGG 2 cut(s) 140, 235
BsmAI GTCTC 2 cut(s) 253, 270
BsnI GGCC 2 cut(s) 261, 282
Bso31I GGTCTC 1 cut(s) 253
Bsp1286I GDGCHC 3 cut(s) 189, 203, 460
Bsp143I GATC 1 cut(s) 363
BspACI CCGC 2 cut(s) 158, 258
BspANI GGCC 2 cut(s) 261, 282
BspCNI CTCAG 1 cut(s) 384
BspFNI CGCG 1 cut(s) 158
BspPI GGATC 1 cut(s) 371
BspTNI GGTCTC 1 cut(s) 253
BsrDI GCAATG 2 cut(s) 151, 351
BsrFI RCCGGY 1 cut(s) 278
BsrI ACTGG 2 cut(s) 262, 331
BssAI RCCGGY 1 cut(s) 278
BssECI CCNNGG 3 cut(s) 202, 243, 373
BssMI GATC 1 cut(s) 363
BssNI GRCGYC 2 cut(s) 32, 290
BssT1I CCWWGG 1 cut(s) 243
Bst6I CTCTTC 2 cut(s) 130, 446
BstACI GRCGYC 2 cut(s) 32, 290
BstC8I GCNNGC 1 cut(s) 237
BstDEI CTNAG 1 cut(s) 392
BstEII GGTNACC 1 cut(s) 377
BstFNI CGCG 1 cut(s) 158
BstKTI GATC 1 cut(s) 366
BstMAI GTCTC 2 cut(s) 253, 270
BstMBI GATC 1 cut(s) 363
BstPAI GACNNNNGTC 1 cut(s) 214
BstPI GGTNACC 1 cut(s) 377
BstSCI CCNGG 2 cut(s) 202, 359
BstSLI GKGCMC 1 cut(s) 203
BstUI CGCG 1 cut(s) 158
BstV1I GCAGC 1 cut(s) 226
BsuI GTATCC 1 cut(s) 452
BsuRI GGCC 2 cut(s) 261, 282
BtsIMutI CAGTG 1 cut(s) 95
Cac8I GCNNGC 1 cut(s) 237
Cfr10I RCCGGY 1 cut(s) 278
Cfr13I GGNCC 1 cut(s) 226
CseI GACGC 2 cut(s) 241, 298
Csp6I GTAC 2 cut(s) 345, 462
CviJI RGCY 8 cut(s) 187, 239, 261, 282, 340, 372, 396, 458
CviKI_1 RGCY 8 cut(s) 187, 239, 261, 282, 340, 372, 396, 458
CviQI GTAC 2 cut(s) 345, 462
DdeI CTNAG 1 cut(s) 392
DpnI GATC 1 cut(s) 365
DpnII GATC 1 cut(s) 363
DrdI GACNNNNNNGTC 1 cut(s) 320
DseDI GACNNNNNNGTC 1 cut(s) 320
EaeI YGGCCR 2 cut(s) 259, 280
Eam1104I CTCTTC 2 cut(s) 130, 446
EarI CTCTTC 2 cut(s) 130, 446
Ecl136II GAGCTC 1 cut(s) 187
Eco130I CCWWGG 1 cut(s) 243
Eco24I GRGCYC 2 cut(s) 189, 460
Eco31I GGTCTC 1 cut(s) 253
Eco47I GGWCC 1 cut(s) 226
Eco53kI GAGCTC 1 cut(s) 187
Eco57I CTGAAG 2 cut(s) 192, 470
Eco91I GGTNACC 1 cut(s) 377
EcoICRI GAGCTC 1 cut(s) 187
EcoO109I RGGNCCY 1 cut(s) 226
EcoO65I GGTNACC 1 cut(s) 377
EcoT14I CCWWGG 1 cut(s) 243
EcoT38I GRGCYC 2 cut(s) 189, 460
ErhI CCWWGG 1 cut(s) 243
FaiI YATR 5 cut(s) 8, 15, 197, 416, 418
FauI CCCGC 1 cut(s) 151
Fnu4HI GCNGC 2 cut(s) 240, 259
FriOI GRGCYC 2 cut(s) 189, 460
Fsp4HI GCNGC 2 cut(s) 240, 259
GluI GCNGC 2 cut(s) 240, 259
HaeIII GGCC 2 cut(s) 261, 282
HapII CCGG 5 cut(s) 51, 190, 204, 279, 360
HgaI GACGC 2 cut(s) 241, 298
Hin1I GRCGYC 2 cut(s) 32, 290
HinfI GANTC 3 cut(s) 37, 153, 314
HpaII CCGG 5 cut(s) 51, 190, 204, 279, 360
HphI GGTGA 1 cut(s) 268
Hpy188I TCNGA 2 cut(s) 405, 450
Hpy188III TCNNGA 2 cut(s) 23, 367
Hpy99I CGWCG 5 cut(s) 34, 37, 213, 292, 326
HpyCH4IV ACGT 2 cut(s) 32, 211
HpyCH4V TGCA 1 cut(s) 10
HpyF3I CTNAG 1 cut(s) 392
HpySE526I ACGT 2 cut(s) 32, 211
Hsp92I GRCGYC 2 cut(s) 32, 290
Kzo9I GATC 1 cut(s) 363
LmnI GCTCC 1 cut(s) 192
Lsp1109I GCAGC 1 cut(s) 226
MaeII ACGT 2 cut(s) 32, 211
MaeIII GTNAC 1 cut(s) 377
MalI GATC 1 cut(s) 365
MboI GATC 1 cut(s) 363
MboII GAAGA 3 cut(s) 117, 162, 463
MhlI GDGCHC 3 cut(s) 189, 203, 460
MluCI AATT 1 cut(s) 129
MlyI GAGTC 1 cut(s) 308
MmeI TCCRAC 1 cut(s) 160
MnlI CCTC 7 cut(s) 284, 317, 328, 367, 383, 392, 447
MseI TTAA 1 cut(s) 222
MspI CCGG 5 cut(s) 51, 190, 204, 279, 360
MspR9I CCNGG 2 cut(s) 204, 361
MvnI CGCG 1 cut(s) 158
NciI CCSGG 2 cut(s) 204, 361
NdeII GATC 1 cut(s) 363
PfeI GAWTC 2 cut(s) 37, 153
PfoI TCCNGGA 1 cut(s) 359
PkrI GCNGC 2 cut(s) 241, 260
PleI GAGTC 1 cut(s) 308
PpsI GAGTC 1 cut(s) 308
PpuMI RGGWCCY 1 cut(s) 226
PshAI GACNNNNGTC 1 cut(s) 214
Psp124BI GAGCTC 1 cut(s) 189
Psp5II RGGWCCY 1 cut(s) 226
PspEI GGTNACC 1 cut(s) 377
PspPI GGNCC 1 cut(s) 226
PspPPI RGGWCCY 1 cut(s) 226
RsaI GTAC 2 cut(s) 346, 463
RsaNI GTAC 2 cut(s) 345, 462
SacI GAGCTC 1 cut(s) 189
SaqAI TTAA 1 cut(s) 222
SatI GCNGC 2 cut(s) 240, 259
Sau3AI GATC 1 cut(s) 363
Sau96I GGNCC 1 cut(s) 226
SchI GAGTC 1 cut(s) 308
ScrFI CCNGG 2 cut(s) 204, 361
SduI GDGCHC 3 cut(s) 189, 203, 460
SetI ASST 9 cut(s) 35, 189, 214, 231, 276, 342, 384, 393, 398
SinI GGWCC 1 cut(s) 226
SmlI CTYRAG 1 cut(s) 21
SmoI CTYRAG 1 cut(s) 21
Sse9I AATT 1 cut(s) 129
SsiI CCGC 2 cut(s) 158, 258
SstI GAGCTC 1 cut(s) 189
StyD4I CCNGG 2 cut(s) 202, 359
StyI CCWWGG 1 cut(s) 243
TaiI ACGT 2 cut(s) 35, 214
TaqI TCGA 1 cut(s) 35
TasI AATT 1 cut(s) 129
TauI GCSGC 1 cut(s) 261
TfiI GAWTC 2 cut(s) 37, 153
Tru1I TTAA 1 cut(s) 222
Tru9I TTAA 1 cut(s) 222
TscAI CASTG 1 cut(s) 102
TseI GCWGC 1 cut(s) 239
TspGWI ACGGA 2 cut(s) 310, 362
TspRI CASTG 1 cut(s) 102
VpaK11BI GGWCC 1 cut(s) 226
ZraI GACGTC 1 cut(s) 33
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.