pycom03g02890

fructose-bisphosphate aldolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
N/A
Physical Location & Seq
Forward (+)
2260828 .. 2262854
2027 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 813 bp
ATGCCCTGGTGGATTTCTGATGGCGTATTCCACCGGAGCTTTGTGTATATAGTGTTATCTGATACCTATCCTAAATCCCCAACCATGTCAGCCTTTGTTGGCAAGTATGCTGAGGAGCTTATAAAGAATGCCAAGTACATTGCCACCCCAGGCAAGGGCATATTGGCAGCAGATGAGAGCACAGGCACCATCGGCAAGCGTCTATCCAGCATCAATGTCGAGAACATTGAGTCCAACCGCCAAGCTCTCCGAGAGCTTCTTTTCACTTCTCCCAACGCCCTCCCTTACCTCTCAGGTGTCATCCTCTTCGAGGAGACCTTGTACCAGAAGAGCTCTGATGGCAAACCCTTCGTCGAAATCCTCCAGGAAAACAATGTCATTCCAGGGATCAAGGTTGACAAGGGCACCGTTGAATTAGCTGGAACAAATGGAGAAACAACAACCCAAGGCTTTGACTCTCTGGGAGCTAGGTGCGCACAGTACTACAAGGCGGGTGCGCGTTTTGCCAAGTGGCGTGCCGTGCTCAAGATAGGCCTCACCGAGCCCTCGGAACTGTCCATCCAGCAGAATGCGCAGGGCTTGGCTCGCTATGCGATCATCTGCCAGGAGAACGGACTAGTGCCAATCGTTGAGCCAGAGATTTTAACTGATGGATCGATCATGACATTAAGAAATGTGCAGCTGCTACTGAAAAGGTTCTTGCAGCAGTTTACAAGGCACTCAATGAGCAACATGTTCTTCTTGAAGGCACACTCCTTAAGCCCAACATGGTTACTCCAGGCTCTGACAGCCCAAAGGTTACGCCAGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0001666 GO:0003674 GO:0003824 GO:0004332 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005975 GO:0005996 GO:0006006 GO:0006082 GO:0006090 GO:0006091 GO:0006094 GO:0006096 GO:0006139 GO:0006163 GO:0006164 GO:0006165 GO:0006725 GO:0006732 GO:0006733 GO:0006753 GO:0006754 GO:0006757 GO:0006793 GO:0006796 GO:0006807 GO:0006950 GO:0008150 GO:0008152 GO:0009056 GO:0009058 GO:0009108 GO:0009117 GO:0009123 GO:0009124 GO:0009126 GO:0009127 GO:0009132 GO:0009135 GO:0009141 GO:0009142 GO:0009144 GO:0009145 GO:0009150 GO:0009152 GO:0009156 GO:0009161 GO:0009165 GO:0009166 GO:0009167 GO:0009168 GO:0009179 GO:0009185 GO:0009199 GO:0009201 GO:0009205 GO:0009206 GO:0009259 GO:0009260 GO:0009507 GO:0009536 GO:0009628 GO:0009987 GO:0016051 GO:0016052 GO:0016053 GO:0016310 GO:0016829 GO:0016830 GO:0016832 GO:0017144 GO:0018130 GO:0019318 GO:0019319 GO:0019359 GO:0019362 GO:0019363 GO:0019438 GO:0019439 GO:0019637 GO:0019693 GO:0019752 GO:0032787 GO:0034404 GO:0034641 GO:0034654 GO:0034655 GO:0036293 GO:0042866 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043436 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044270 GO:0044271 GO:0044281 GO:0044283 GO:0044424 GO:0044444 GO:0044464 GO:0046031 GO:0046034 GO:0046364 GO:0046390 GO:0046394 GO:0046434 GO:0046483 GO:0046496 GO:0046700 GO:0046939 GO:0050896 GO:0051186 GO:0051188 GO:0055086 GO:0070482 GO:0071704 GO:0072330 GO:0072521 GO:0072522 GO:0072524 GO:0072525 GO:0090407 GO:1901135 GO:1901137 GO:1901292 GO:1901293 GO:1901360 GO:1901361 GO:1901362 GO:1901564 GO:1901566 GO:1901575 GO:1901576
KEGG Pathways
Metabolic & Signaling
ko00010 ko00030 ko00051 ko00680 ko00710 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00010 map00030 map00051 map00680 map00710 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
Glycolytic

Protein Analysis

271

Amino Acids

30.11

Weight (kDa)

9.13

Isoelectric Point (pI)

44.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glycolytic PF00274 39 - 254 4.5e-91 Fructose-bisphosphate aldolase class-I
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 122
Acc16I TGCGCA 2 cut(s) 475, 573
AccB1I GGYRCC 2 cut(s) 185, 404
AccII CGCG 1 cut(s) 499
AciI CCGC 2 cut(s) 238, 491
AclWI GGATC 2 cut(s) 395, 661
AfaI GTAC 3 cut(s) 137, 323, 482
AfiI CCNNNNNNNGG 3 cut(s) 154, 155, 310
AflII CTTAAG 1 cut(s) 757
AflIII ACRYGT 1 cut(s) 732
AgsI TTSAA 2 cut(s) 413, 745
AhlI ACTAGT 1 cut(s) 616
AjnI CCWGG 6 cut(s) 5, 148, 363, 382, 603, 777
AloI GAACNNNNNNTCC 4 cut(s) 215, 247, 543, 575
AluBI AGCT 8 cut(s) 39, 118, 245, 256, 333, 419, 467, 682
AluI AGCT 8 cut(s) 39, 118, 245, 256, 333, 419, 467, 682
Alw21I GWGCWC 3 cut(s) 182, 335, 525
Alw26I GTCTC 1 cut(s) 308
AlwI GGATC 2 cut(s) 395, 661
AlwNI CAGNNNCTG 1 cut(s) 784
AoxI GGCC 1 cut(s) 532
ApeKI GCWGC 4 cut(s) 167, 679, 682, 703
ArsI GACNNNNNNTTYG 2 cut(s) 336, 368
Asp700I GAANNNNTTC 1 cut(s) 695
AspLEI GCGC 3 cut(s) 476, 499, 574
AsuHPI GGTGA 1 cut(s) 529
BaeGI GKGCMC 1 cut(s) 407
BanI GGYRCC 2 cut(s) 185, 404
BanII GRGCYC 2 cut(s) 335, 546
Bbv12I GWGCWC 3 cut(s) 182, 335, 525
BbvCI CCTCAGC 1 cut(s) 111
BbvI GCAGC 4 cut(s) 179, 669, 691, 715
BccI CCATC 5 cut(s) 14, 197, 332, 566, 644
BceAI ACGGC 1 cut(s) 503
BcgI CGANNNNNNTGC 4 cut(s) 199, 233, 331, 365
BciT130I CCWGG 6 cut(s) 7, 150, 365, 384, 605, 779
BcoDI GTCTC 1 cut(s) 308
BcuI ACTAGT 1 cut(s) 616
BfaI CTAG 2 cut(s) 468, 617
BfrI CTTAAG 1 cut(s) 757
BisI GCNGC 4 cut(s) 168, 680, 683, 704
BlsI GCNGC 4 cut(s) 169, 681, 684, 705
BmcAI AGTACT 1 cut(s) 482
Bme1390I CCNGG 6 cut(s) 7, 150, 365, 384, 605, 779
BmiI GGNNCC 2 cut(s) 187, 406
BmrFI CCNGG 6 cut(s) 7, 150, 365, 384, 605, 779
BmsI GCATC 1 cut(s) 219
BpmI CTGGAG 2 cut(s) 347, 761
Bpu10I CCTNAGC 1 cut(s) 111
BpuEI CTTGAG 1 cut(s) 509
Bsa29I ATCGAT 1 cut(s) 656
BsaBI GATNNNNATC 1 cut(s) 66
BsaI GGTCTC 1 cut(s) 308
BsaJI CCNNGG 5 cut(s) 5, 148, 383, 445, 546
BsaWI WCCGGW 1 cut(s) 33
BsaXI ACNNNNNCTCC 2 cut(s) 305, 335
Bsc4I CCNNNNNNNGG 3 cut(s) 154, 155, 310
Bse3DI GCAATG 1 cut(s) 138
Bse8I GATNNNNATC 1 cut(s) 66
BseBI CCWGG 6 cut(s) 7, 150, 365, 384, 605, 779
BseCI ATCGAT 1 cut(s) 656
BseDI CCNNGG 5 cut(s) 5, 148, 383, 445, 546
BseGI GGATG 2 cut(s) 300, 558
BseJI GATNNNNATC 1 cut(s) 66
BseLI CCNNNNNNNGG 3 cut(s) 154, 155, 310
BseMI GCAATG 1 cut(s) 138
BseMII CTCAG 2 cut(s) 102, 306
BseRI GAGGAG 2 cut(s) 128, 326
BseSI GKGCMC 1 cut(s) 407
BseXI GCAGC 4 cut(s) 179, 669, 691, 715
BsgI GTGCAG 1 cut(s) 698
Bsh1236I CGCG 1 cut(s) 499
BshFI GGCC 1 cut(s) 534
BshNI GGYRCC 2 cut(s) 185, 404
BshVI ATCGAT 1 cut(s) 656
BsiHKAI GWGCWC 3 cut(s) 182, 335, 525
BsiSI CCGG 1 cut(s) 34
BslI CCNNNNNNNGG 3 cut(s) 154, 155, 310
BsmAI GTCTC 1 cut(s) 308
BsmI GAATGC 2 cut(s) 133, 574
BsnI GGCC 1 cut(s) 534
Bso31I GGTCTC 1 cut(s) 308
Bsp1286I GDGCHC 5 cut(s) 182, 335, 407, 525, 546
Bsp143I GATC 4 cut(s) 387, 594, 653, 657
BspACI CCGC 2 cut(s) 238, 491
BspANI GGCC 1 cut(s) 534
BspCNI CTCAG 2 cut(s) 103, 305
BspDI ATCGAT 1 cut(s) 656
BspFNI CGCG 1 cut(s) 499
BspHI TCATGA 1 cut(s) 660
BspLI GGNNCC 2 cut(s) 187, 406
BspPI GGATC 2 cut(s) 395, 661
BspQI GCTCTTC 1 cut(s) 323
BspT107I GGYRCC 2 cut(s) 185, 404
BspTI CTTAAG 1 cut(s) 757
BspTNI GGTCTC 1 cut(s) 308
BsrDI GCAATG 1 cut(s) 138
BssECI CCNNGG 5 cut(s) 5, 148, 383, 445, 546
BssMI GATC 4 cut(s) 387, 594, 653, 657
BssT1I CCWWGG 1 cut(s) 445
Bst2UI CCWGG 6 cut(s) 7, 150, 365, 384, 605, 779
Bst4CI ACNGT 3 cut(s) 409, 480, 555
Bst6I CTCTTC 2 cut(s) 311, 323
BstAFI CTTAAG 1 cut(s) 757
BstC8I GCNNGC 3 cut(s) 197, 516, 586
BstDEI CTNAG 2 cut(s) 111, 292
BstENI CCTNNNNNAGG 1 cut(s) 308
BstF5I GGATG 2 cut(s) 300, 558
BstFNI CGCG 1 cut(s) 499
BstHHI GCGC 3 cut(s) 476, 499, 574
BstKTI GATC 4 cut(s) 390, 597, 656, 660
BstMAI GTCTC 1 cut(s) 308
BstMBI GATC 4 cut(s) 387, 594, 653, 657
BstMWI GCNNNNNNNGC 9 cut(s) 192, 339, 473, 503, 520, 571, 585, 590, 788
BstNI CCWGG 6 cut(s) 7, 150, 365, 384, 605, 779
BstNSI RCATGY 1 cut(s) 736
BstSCI CCNGG 6 cut(s) 5, 148, 363, 382, 603, 777
BstSLI GKGCMC 1 cut(s) 407
BstUI CGCG 1 cut(s) 499
BstV1I GCAGC 4 cut(s) 179, 669, 691, 715
Bsu15I ATCGAT 1 cut(s) 656
BsuRI GGCC 1 cut(s) 534
BsuTUI ATCGAT 1 cut(s) 656
BtsCI GGATG 2 cut(s) 300, 558
Cac8I GCNNGC 3 cut(s) 197, 516, 586
CaiI CAGNNNCTG 1 cut(s) 784
CciI TCATGA 1 cut(s) 660
CfoI GCGC 3 cut(s) 476, 499, 574
ClaI ATCGAT 1 cut(s) 656
CseI GACGC 1 cut(s) 188
Csp6I GTAC 3 cut(s) 136, 322, 481
CviAII CATG 4 cut(s) 85, 661, 733, 768
CviQI GTAC 3 cut(s) 136, 322, 481
DdeI CTNAG 2 cut(s) 111, 292
DpnI GATC 4 cut(s) 389, 596, 655, 659
DpnII GATC 4 cut(s) 387, 594, 653, 657
Eam1104I CTCTTC 2 cut(s) 311, 323
EarI CTCTTC 2 cut(s) 311, 323
Ecl136II GAGCTC 1 cut(s) 333
Eco130I CCWWGG 1 cut(s) 445
Eco147I AGGCCT 1 cut(s) 534
Eco24I GRGCYC 2 cut(s) 335, 546
Eco31I GGTCTC 1 cut(s) 308
Eco53kI GAGCTC 1 cut(s) 333
EcoICRI GAGCTC 1 cut(s) 333
EcoNI CCTNNNNNAGG 1 cut(s) 308
EcoRII CCWGG 6 cut(s) 5, 148, 363, 382, 603, 777
EcoT14I CCWWGG 1 cut(s) 445
EcoT38I GRGCYC 2 cut(s) 335, 546
ErhI CCWWGG 1 cut(s) 445
FaeI CATG 4 cut(s) 88, 664, 736, 771
FatI CATG 4 cut(s) 84, 660, 732, 767
FauI CCCGC 1 cut(s) 484
Fnu4HI GCNGC 4 cut(s) 168, 680, 683, 704
FokI GGATG 2 cut(s) 287, 545
FriOI GRGCYC 2 cut(s) 335, 546
Fsp4HI GCNGC 4 cut(s) 168, 680, 683, 704
FspAI RTGCGCAY 1 cut(s) 475
FspBI CTAG 2 cut(s) 468, 617
FspI TGCGCA 2 cut(s) 475, 573
GlaI GCGC 3 cut(s) 475, 498, 573
GluI GCNGC 4 cut(s) 168, 680, 683, 704
GsuI CTGGAG 2 cut(s) 347, 761
HaeIII GGCC 1 cut(s) 534
HapII CCGG 1 cut(s) 34
HgaI GACGC 1 cut(s) 188
HhaI GCGC 3 cut(s) 476, 499, 574
Hin1II CATG 4 cut(s) 88, 664, 736, 771
Hin6I GCGC 3 cut(s) 474, 497, 572
HinP1I GCGC 3 cut(s) 474, 497, 572
HincII GTYRAC 1 cut(s) 397
HindII GTYRAC 1 cut(s) 397
HinfI GANTC 2 cut(s) 230, 455
HpaII CCGG 1 cut(s) 34
HphI GGTGA 1 cut(s) 529
Hpy166II GTNNAC 2 cut(s) 397, 711
Hpy188I TCNGA 6 cut(s) 19, 61, 251, 337, 550, 786
Hpy188III TCNNGA 4 cut(s) 220, 526, 661, 742
Hpy8I GTNNAC 2 cut(s) 397, 711
Hpy99I CGWCG 1 cut(s) 356
HpyAV CCTTC 2 cut(s) 358, 739
HpyCH4III ACNGT 3 cut(s) 409, 480, 555
HpyCH4V TGCA 2 cut(s) 679, 703
HpyF10VI GCNNNNNNNGC 9 cut(s) 192, 339, 473, 503, 520, 571, 585, 590, 788
HpyF3I CTNAG 2 cut(s) 111, 292
Hsp92II CATG 4 cut(s) 88, 664, 736, 771
HspAI GCGC 3 cut(s) 474, 497, 572
Kzo9I GATC 4 cut(s) 387, 594, 653, 657
LguI GCTCTTC 1 cut(s) 323
LmnI GCTCC 3 cut(s) 36, 115, 464
Lsp1109I GCAGC 4 cut(s) 179, 669, 691, 715
LweI GCATC 1 cut(s) 219
MaeI CTAG 2 cut(s) 468, 617
MaeIII GTNAC 2 cut(s) 771, 798
MalI GATC 4 cut(s) 389, 596, 655, 659
MboI GATC 4 cut(s) 387, 594, 653, 657
MboII GAAGA 3 cut(s) 298, 340, 730
MhlI GDGCHC 5 cut(s) 182, 335, 407, 525, 546
MluCI AATT 1 cut(s) 413
MlyI GAGTC 2 cut(s) 239, 449
MmeI TCCRAC 1 cut(s) 258
MnlI CCTC 9 cut(s) 106, 290, 299, 304, 314, 371, 545, 556, 801
MroXI GAANNNNTTC 1 cut(s) 695
MseI TTAA 3 cut(s) 644, 668, 758
MspA1I CMGCKG 1 cut(s) 682
MspCI CTTAAG 1 cut(s) 757
MspI CCGG 1 cut(s) 34
MspR9I CCNGG 6 cut(s) 7, 150, 365, 384, 605, 779
Mva1269I GAATGC 2 cut(s) 133, 574
MvaI CCWGG 6 cut(s) 7, 150, 365, 384, 605, 779
MvnI CGCG 1 cut(s) 499
MwoI GCNNNNNNNGC 9 cut(s) 192, 339, 473, 503, 520, 571, 585, 590, 788
NdeII GATC 4 cut(s) 387, 594, 653, 657
NlaIII CATG 4 cut(s) 88, 664, 736, 771
NlaIV GGNNCC 2 cut(s) 187, 406
NsbI TGCGCA 2 cut(s) 475, 573
NspI RCATGY 1 cut(s) 736
PagI TCATGA 1 cut(s) 660
PceI AGGCCT 1 cut(s) 534
PciI ACATGT 1 cut(s) 732
PciSI GCTCTTC 1 cut(s) 323
PctI GAATGC 2 cut(s) 133, 574
PdmI GAANNNNTTC 1 cut(s) 695
PfoI TCCNGGA 1 cut(s) 363
PkrI GCNGC 4 cut(s) 169, 681, 684, 705
PleI GAGTC 2 cut(s) 238, 449
PpsI GAGTC 2 cut(s) 238, 449
PscI ACATGT 1 cut(s) 732
PsiI TTATAA 1 cut(s) 122
Psp124BI GAGCTC 1 cut(s) 335
Psp6I CCWGG 6 cut(s) 5, 148, 363, 382, 603, 777
PspGI CCWGG 6 cut(s) 5, 148, 363, 382, 603, 777
PspN4I GGNNCC 2 cut(s) 187, 406
PstNI CAGNNNCTG 1 cut(s) 784
PvuII CAGCTG 1 cut(s) 682
RsaI GTAC 3 cut(s) 137, 323, 482
RsaNI GTAC 3 cut(s) 136, 322, 481
SacI GAGCTC 1 cut(s) 335
SapI GCTCTTC 1 cut(s) 323
SaqAI TTAA 3 cut(s) 644, 668, 758
SatI GCNGC 4 cut(s) 168, 680, 683, 704
Sau3AI GATC 4 cut(s) 387, 594, 653, 657
ScaI AGTACT 1 cut(s) 482
SchI GAGTC 2 cut(s) 239, 449
ScrFI CCNGG 6 cut(s) 7, 150, 365, 384, 605, 779
SduI GDGCHC 5 cut(s) 182, 335, 407, 525, 546
SfaNI GCATC 1 cut(s) 219
SmlI CTYRAG 2 cut(s) 524, 757
SmoI CTYRAG 2 cut(s) 524, 757
SpeI ACTAGT 1 cut(s) 616
Sse9I AATT 1 cut(s) 413
SseBI AGGCCT 1 cut(s) 534
SsiI CCGC 2 cut(s) 238, 491
SspMI CTAG 2 cut(s) 468, 617
SstI GAGCTC 1 cut(s) 335
StuI AGGCCT 1 cut(s) 534
StyD4I CCNGG 6 cut(s) 5, 148, 363, 382, 603, 777
StyI CCWWGG 1 cut(s) 445
TaaI ACNGT 3 cut(s) 409, 480, 555
TaqI TCGA 4 cut(s) 219, 309, 354, 656
TasI AATT 1 cut(s) 413
TatI WGTACW 2 cut(s) 135, 480
Tru1I TTAA 3 cut(s) 644, 668, 758
Tru9I TTAA 3 cut(s) 644, 668, 758
TseI GCWGC 4 cut(s) 167, 679, 682, 703
TspGWI ACGGA 1 cut(s) 627
Vha464I CTTAAG 1 cut(s) 757
XagI CCTNNNNNAGG 1 cut(s) 308
XceI RCATGY 1 cut(s) 736
XmnI GAANNNNTTC 1 cut(s) 695
XspI CTAG 2 cut(s) 468, 617
ZrmI AGTACT 1 cut(s) 482
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.