pycom03g03830
ERF Family

Belongs to the protein kinase superfamily. Ser Thr protein kinase family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
N/A
Physical Location & Seq
Reverse (-)
3026471 .. 3029327
2857 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1035 bp
ATGTGGAACAAAATAAGTGGAAGTATACCAAAGGAGATAGGCAACATTACATCCTTGGAACTTTTGCTCCTTAATGGAAACCAGTTATCAGGTTCCTTGCCCGAAAAGCTCGGTTATCTTCCAAACTTGGACAGACTACAAATTGACCAGAACCAAATATCGGGATCATTACCTACATCGTTTGCAAACTTAAACAAGACAAGTGTAATATCATATTGTGTTTGTTATACTTCTCTCTTCCTTATGTTTCGTGTCTTTTCCCTTTTGAGCAGTCACATGAACAACAATTCAATAAGCGGGCAAATTCCACCCGAGCTTTCCATATTACCAATGCTTAAGCACTTTGTAAGAACGGGAGTGAGAAAAGTTGTTACTTCTGTTGTGTGTGTGAATTTAAACTCTCTAGTGGAGATGCAGAACAATAACCTTACAAATATTACCGGCAGTACTGTACTTCCGCAAAATGTCACTGTCTGGTTTGCTTCTGGACTTTGTTCTCTGTTAAGGCTTCGGGGAAATCCGGTTTGCTTAAATGGCAACCTTGATAACTTCTGTGGAACCGAAAGTGATGACGAAAGTGATAGTCAGATTTTGACAAACTCCACTGCCAACTGTGTGAATCAAGCATGTCCACCCCCTTTCGAATATATTCCTGTAGCCTGTTTTTGTGCTGCTCCTCTAATTGTTGACATTCGGTTGAAAAGTCCTGCATTCACAGATTTTCGCCCTTACGAAAGTACATTTGAGCAGTATCTTACTTCTGGTATCAAATTAGATCTCGATCAGCTGGACATTGCTTCGTTTGTGTGGGAAAAAGGGCCTCGTCTGAGAATATCTCTGAAGCTTTTTCCTGTATATGTTGATAATGCCAATAATTCCCATACTTTCAATAGAACTGAACTTAAAAGAATCATGAATATGTTCGCATCATGGGACATTCCTGAAAGTGGCCTATTTGGACCCTATGAACTTCTCAACTTCATTCTACTGGATCCTTACAAGGACGGTTTGTCTCAAAAGCCTTATTTTTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0016020 GO:0044464 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_8 Pkinase Pkinase_Tyr

Protein Analysis

345

Amino Acids

38.44

Weight (kDa)

5.48

Isoelectric Point (pI)

28.74

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 25
AciI CCGC 2 cut(s) 297, 458
AclWI GGATC 3 cut(s) 172, 986, 999
AcsI RAATTY 2 cut(s) 303, 391
AcuI CTGAAG 1 cut(s) 860
AfaI GTAC 3 cut(s) 448, 453, 739
AfiI CCNNNNNNNGG 2 cut(s) 160, 947
AflII CTTAAG 1 cut(s) 335
AgsI TTSAA 3 cut(s) 291, 700, 889
AhdI GACNNNNNGTC 1 cut(s) 1009
AloI GAACNNNNNNTCC 2 cut(s) 51, 83
AluBI AGCT 4 cut(s) 109, 316, 787, 844
AluI AGCT 4 cut(s) 109, 316, 787, 844
Alw26I GTCTC 1 cut(s) 1017
AlwI GGATC 3 cut(s) 172, 986, 999
Ama87I CYCGRG 1 cut(s) 311
AoxI GGCC 2 cut(s) 818, 949
ApeKI GCWGC 1 cut(s) 671
ApoI RAATTY 2 cut(s) 303, 391
Asp700I GAANNNNTTC 2 cut(s) 648, 920
AspS9I GGNCC 2 cut(s) 818, 959
AsuII TTCGAA 1 cut(s) 642
AvaI CYCGRG 1 cut(s) 311
AvaII GGWCC 1 cut(s) 959
BamHI GGATCC 1 cut(s) 991
BbvI GCAGC 1 cut(s) 658
BcoDI GTCTC 1 cut(s) 1017
BfaI CTAG 2 cut(s) 404, 1033
BfmI CTRYAG 1 cut(s) 654
BfrI CTTAAG 1 cut(s) 335
BglII AGATCT 1 cut(s) 775
BisI GCNGC 1 cut(s) 672
BlsI GCNGC 1 cut(s) 673
BmcAI AGTACT 1 cut(s) 448
Bme18I GGWCC 1 cut(s) 959
BmeRI GACNNNNNGTC 1 cut(s) 1009
BmeT110I CYCGRG 1 cut(s) 311
BmgT120I GGNCC 2 cut(s) 818, 959
BmiI GGNNCC 4 cut(s) 94, 559, 961, 993
BmsI GCATC 2 cut(s) 402, 935
BplI GAGNNNNNCTC 2 cut(s) 820, 852
Bpu14I TTCGAA 1 cut(s) 642
BsaBI GATNNNNATC 1 cut(s) 780
BsaJI CCNNGG 1 cut(s) 54
BsaWI WCCGGW 1 cut(s) 520
BsaXI ACNNNNNCTCC 2 cut(s) 51, 81
Bsc4I CCNNNNNNNGG 2 cut(s) 160, 947
Bse118I RCCGGY 1 cut(s) 440
Bse1I ACTGG 2 cut(s) 82, 993
Bse3DI GCAATG 1 cut(s) 792
Bse8I GATNNNNATC 1 cut(s) 780
BseDI CCNNGG 1 cut(s) 54
BseGI GGATG 1 cut(s) 50
BseJI GATNNNNATC 1 cut(s) 780
BseLI CCNNNNNNNGG 2 cut(s) 160, 947
BseMI GCAATG 1 cut(s) 792
BseMII CTCAG 1 cut(s) 818
BseNI ACTGG 2 cut(s) 82, 993
BseRI GAGGAG 1 cut(s) 666
BseXI GCAGC 1 cut(s) 658
BshFI GGCC 2 cut(s) 820, 951
BsiHKCI CYCGRG 1 cut(s) 311
BsiSI CCGG 2 cut(s) 441, 521
BslFI GGGAC 1 cut(s) 947
BslI CCNNNNNNNGG 2 cut(s) 160, 947
BsmAI GTCTC 1 cut(s) 1017
BsmFI GGGAC 1 cut(s) 947
BsmI GAATGC 1 cut(s) 710
BsnI GGCC 2 cut(s) 820, 951
BsoBI CYCGRG 1 cut(s) 311
Bsp119I TTCGAA 1 cut(s) 642
Bsp143I GATC 4 cut(s) 164, 775, 781, 991
BspACI CCGC 2 cut(s) 297, 458
BspANI GGCC 2 cut(s) 820, 951
BspCNI CTCAG 1 cut(s) 819
BspHI TCATGA 1 cut(s) 912
BspLI GGNNCC 4 cut(s) 94, 559, 961, 993
BspPI GGATC 3 cut(s) 172, 986, 999
BspT104I TTCGAA 1 cut(s) 642
BspTI CTTAAG 1 cut(s) 335
BsrDI GCAATG 1 cut(s) 792
BsrFI RCCGGY 1 cut(s) 440
BsrI ACTGG 2 cut(s) 82, 993
BssAI RCCGGY 1 cut(s) 440
BssECI CCNNGG 1 cut(s) 54
BssMI GATC 4 cut(s) 164, 775, 781, 991
BssNAI GTATAC 1 cut(s) 26
BssT1I CCWWGG 1 cut(s) 54
Bst1107I GTATAC 1 cut(s) 26
Bst4CI ACNGT 4 cut(s) 451, 472, 614, 1007
Bst6I CTCTTC 1 cut(s) 242
BstAFI CTTAAG 1 cut(s) 335
BstBI TTCGAA 1 cut(s) 642
BstC8I GCNNGC 1 cut(s) 299
BstDEI CTNAG 1 cut(s) 827
BstF5I GGATG 1 cut(s) 50
BstKTI GATC 4 cut(s) 167, 778, 784, 994
BstMAI GTCTC 1 cut(s) 1017
BstMBI GATC 4 cut(s) 164, 775, 781, 991
BstMWI GCNNNNNNNGC 2 cut(s) 106, 534
BstNSI RCATGY 1 cut(s) 630
BstSFI CTRYAG 1 cut(s) 654
BstV1I GCAGC 1 cut(s) 658
BstX2I RGATCY 2 cut(s) 775, 991
BstYI RGATCY 2 cut(s) 775, 991
BstZ17I GTATAC 1 cut(s) 26
BsuRI GGCC 2 cut(s) 820, 951
BtsCI GGATG 1 cut(s) 50
BtsI GCAGTG 1 cut(s) 603
BtsIMutI CAGTG 2 cut(s) 468, 603
Cac8I GCNNGC 1 cut(s) 299
CciI TCATGA 1 cut(s) 912
Cfr10I RCCGGY 1 cut(s) 440
Cfr13I GGNCC 2 cut(s) 818, 959
Csp6I GTAC 3 cut(s) 447, 452, 738
CspCI CAANNNNNGTGG 1 cut(s) 33
CviAII CATG 4 cut(s) 277, 627, 913, 930
CviJI RGCY 9 cut(s) 109, 316, 508, 659, 787, 820, 844, 951, 1021
CviKI_1 RGCY 9 cut(s) 109, 316, 508, 659, 787, 820, 844, 951, 1021
CviQI GTAC 3 cut(s) 447, 452, 738
DdeI CTNAG 1 cut(s) 827
DpnI GATC 4 cut(s) 166, 777, 783, 993
DpnII GATC 4 cut(s) 164, 775, 781, 991
DraI TTTAAA 1 cut(s) 396
DriI GACNNNNNGTC 1 cut(s) 1009
Eam1104I CTCTTC 1 cut(s) 242
Eam1105I GACNNNNNGTC 1 cut(s) 1009
EarI CTCTTC 1 cut(s) 242
Eco130I CCWWGG 1 cut(s) 54
Eco47I GGWCC 1 cut(s) 959
Eco57I CTGAAG 1 cut(s) 860
Eco88I CYCGRG 1 cut(s) 311
EcoO109I RGGNCCY 1 cut(s) 818
EcoT14I CCWWGG 1 cut(s) 54
ErhI CCWWGG 1 cut(s) 54
FaeI CATG 4 cut(s) 280, 630, 916, 933
FaqI GGGAC 1 cut(s) 947
FatI CATG 4 cut(s) 276, 626, 912, 929
FauI CCCGC 1 cut(s) 290
FblI GTMKAC 1 cut(s) 25
Fnu4HI GCNGC 1 cut(s) 672
FokI GGATG 1 cut(s) 37
Fsp4HI GCNGC 1 cut(s) 672
FspBI CTAG 2 cut(s) 404, 1033
GluI GCNGC 1 cut(s) 672
HaeIII GGCC 2 cut(s) 820, 951
HapII CCGG 2 cut(s) 441, 521
Hin1II CATG 4 cut(s) 280, 630, 916, 933
HincII GTYRAC 1 cut(s) 688
HindII GTYRAC 1 cut(s) 688
HindIII AAGCTT 1 cut(s) 842
HinfI GANTC 2 cut(s) 619, 909
HpaII CCGG 2 cut(s) 441, 521
Hpy166II GTNNAC 3 cut(s) 26, 632, 688
Hpy188I TCNGA 3 cut(s) 588, 828, 840
Hpy188III TCNNGA 5 cut(s) 162, 486, 779, 913, 941
Hpy8I GTNNAC 3 cut(s) 26, 632, 688
HpyCH4III ACNGT 4 cut(s) 451, 472, 614, 1007
HpyCH4V TGCA 3 cut(s) 185, 415, 710
HpyF10VI GCNNNNNNNGC 2 cut(s) 106, 534
HpyF3I CTNAG 1 cut(s) 827
Hsp92II CATG 4 cut(s) 280, 630, 916, 933
Kzo9I GATC 4 cut(s) 164, 775, 781, 991
LmnI GCTCC 2 cut(s) 72, 679
Lsp1109I GCAGC 1 cut(s) 658
LweI GCATC 2 cut(s) 402, 935
MaeI CTAG 2 cut(s) 404, 1033
MaeIII GTNAC 3 cut(s) 272, 370, 466
MalI GATC 4 cut(s) 166, 777, 783, 993
MboI GATC 4 cut(s) 164, 775, 781, 991
MboII GAAGA 2 cut(s) 110, 229
MflI RGATCY 2 cut(s) 775, 991
MluCI AATT 7 cut(s) 141, 286, 303, 391, 681, 770, 874
MnlI CCTC 2 cut(s) 687, 831
MroXI GAANNNNTTC 2 cut(s) 648, 920
MseI TTAA 7 cut(s) 72, 191, 336, 395, 503, 530, 903
MslI CAYNNNNRTG 1 cut(s) 917
MspA1I CMGCKG 1 cut(s) 787
MspCI CTTAAG 1 cut(s) 335
MspI CCGG 2 cut(s) 441, 521
Mva1269I GAATGC 1 cut(s) 710
MwoI GCNNNNNNNGC 2 cut(s) 106, 534
NdeII GATC 4 cut(s) 164, 775, 781, 991
NlaIII CATG 4 cut(s) 280, 630, 916, 933
NlaIV GGNNCC 4 cut(s) 94, 559, 961, 993
NmuCI GTSAC 2 cut(s) 272, 466
NspI RCATGY 1 cut(s) 630
NspV TTCGAA 1 cut(s) 642
PagI TCATGA 1 cut(s) 912
PctI GAATGC 1 cut(s) 710
PdmI GAANNNNTTC 2 cut(s) 648, 920
PfeI GAWTC 2 cut(s) 619, 909
PkrI GCNGC 1 cut(s) 673
PspN4I GGNNCC 4 cut(s) 94, 559, 961, 993
PspPI GGNCC 2 cut(s) 818, 959
PsuI RGATCY 2 cut(s) 775, 991
PvuII CAGCTG 1 cut(s) 787
RsaI GTAC 3 cut(s) 448, 453, 739
RsaNI GTAC 3 cut(s) 447, 452, 738
RseI CAYNNNNRTG 1 cut(s) 917
SaqAI TTAA 7 cut(s) 72, 191, 336, 395, 503, 530, 903
SatI GCNGC 1 cut(s) 672
Sau3AI GATC 4 cut(s) 164, 775, 781, 991
Sau96I GGNCC 2 cut(s) 818, 959
ScaI AGTACT 1 cut(s) 448
SetI ASST 8 cut(s) 94, 111, 175, 318, 429, 543, 789, 846
SfaNI GCATC 2 cut(s) 402, 935
SfcI CTRYAG 1 cut(s) 654
SfuI TTCGAA 1 cut(s) 642
SinI GGWCC 1 cut(s) 959
SmiMI CAYNNNNRTG 1 cut(s) 917
SmlI CTYRAG 1 cut(s) 335
SmoI CTYRAG 1 cut(s) 335
Sse9I AATT 7 cut(s) 141, 286, 303, 391, 681, 770, 874
SsiI CCGC 2 cut(s) 297, 458
SspI AATATT 1 cut(s) 436
SspMI CTAG 2 cut(s) 404, 1033
StyI CCWWGG 1 cut(s) 54
TaaI ACNGT 4 cut(s) 451, 472, 614, 1007
TaqI TCGA 2 cut(s) 642, 780
TasI AATT 7 cut(s) 141, 286, 303, 391, 681, 770, 874
TatI WGTACW 3 cut(s) 446, 451, 737
TfiI GAWTC 2 cut(s) 619, 909
Tru1I TTAA 7 cut(s) 72, 191, 336, 395, 503, 530, 903
Tru9I TTAA 7 cut(s) 72, 191, 336, 395, 503, 530, 903
TscAI CASTG 2 cut(s) 475, 610
TseFI GTSAC 2 cut(s) 272, 466
TseI GCWGC 1 cut(s) 671
Tsp45I GTSAC 2 cut(s) 272, 466
TspDTI ATGAA 4 cut(s) 293, 929, 970, 981
TspRI CASTG 2 cut(s) 475, 610
Vha464I CTTAAG 1 cut(s) 335
VpaK11BI GGWCC 1 cut(s) 959
XapI RAATTY 2 cut(s) 303, 391
XceI RCATGY 1 cut(s) 630
XmiI GTMKAC 1 cut(s) 25
XmnI GAANNNNTTC 2 cut(s) 648, 920
XspI CTAG 2 cut(s) 404, 1033
ZrmI AGTACT 1 cut(s) 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.