pycom03g04190

Subtilisin-like serine endopeptidase family protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
N/A
Physical Location & Seq
Reverse (-)
3273145 .. 3273555
411 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 306 bp
ATGTCGGTGCAGGTCCATATTGTGTACTTGGGGTCACTCTTCCTGATGGTGTTTACTCACCAGTCTCAACACCTTAGAGTTCTACAGAGAGTTCTCGGGCCGGGAAGATATGTAGTCTCTGTTCTTTTAAGCAGAAAATTCAAGCTGCAAACAACAAGATCTTCGGAATTTATGGGATTAAGTGATAAAGTTAGTGGAAATGCCACTATCGAGAGTGACTTTATTGTCGTTGTTATCGGCAGTGGGATTTGGCCAGAATCTAAGAGCTTTAAAACAAATGTTTTGGTCCTCCTCCCTAAAGGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005576
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Inhibitor_I9 PA Peptidase_S8

Protein Analysis

102

Amino Acids

11.24

Weight (kDa)

10.2

Isoelectric Point (pI)

40.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 224
AcoI YGGCCR 1 cut(s) 251
AcsI RAATTY 2 cut(s) 137, 167
AfaI GTAC 1 cut(s) 26
AgsI TTSAA 1 cut(s) 142
AluBI AGCT 2 cut(s) 145, 267
AluI AGCT 2 cut(s) 145, 267
Alw26I GTCTC 2 cut(s) 69, 121
Ama87I CYCGRG 1 cut(s) 95
AoxI GGCC 2 cut(s) 98, 251
ApeKI GCWGC 1 cut(s) 145
ApoI RAATTY 2 cut(s) 137, 167
AspS9I GGNCC 3 cut(s) 13, 98, 286
AsuC2I CCSGG 1 cut(s) 102
AsuHPI GGTGA 1 cut(s) 50
AvaI CYCGRG 1 cut(s) 95
AvaII GGWCC 2 cut(s) 13, 286
BalI TGGCCA 1 cut(s) 253
BbvI GCAGC 1 cut(s) 132
BccI CCATC 1 cut(s) 40
BcnI CCSGG 1 cut(s) 102
BcoDI GTCTC 2 cut(s) 69, 121
BfmI CTRYAG 1 cut(s) 83
BglII AGATCT 1 cut(s) 158
BisI GCNGC 1 cut(s) 146
BlsI GCNGC 1 cut(s) 147
Bme1390I CCNGG 1 cut(s) 102
Bme18I GGWCC 2 cut(s) 13, 286
BmeT110I CYCGRG 1 cut(s) 95
BmgT120I GGNCC 3 cut(s) 13, 98, 286
BmrFI CCNGG 1 cut(s) 102
BpuMI CCSGG 1 cut(s) 102
Bse1I ACTGG 1 cut(s) 61
BseNI ACTGG 1 cut(s) 61
BseRI GAGGAG 1 cut(s) 281
BseXI GCAGC 1 cut(s) 132
BsgI GTGCAG 1 cut(s) 29
BshFI GGCC 2 cut(s) 100, 253
BsiHKCI CYCGRG 1 cut(s) 95
BsiSI CCGG 1 cut(s) 101
BsmAI GTCTC 2 cut(s) 69, 121
BsnI GGCC 2 cut(s) 100, 253
BsoBI CYCGRG 1 cut(s) 95
Bsp143I GATC 1 cut(s) 158
BspANI GGCC 2 cut(s) 100, 253
BsrI ACTGG 1 cut(s) 61
BssMI GATC 1 cut(s) 158
Bst6I CTCTTC 1 cut(s) 44
BstDEI CTNAG 2 cut(s) 74, 261
BstKTI GATC 1 cut(s) 161
BstMAI GTCTC 2 cut(s) 69, 121
BstMBI GATC 1 cut(s) 158
BstSCI CCNGG 1 cut(s) 100
BstSFI CTRYAG 1 cut(s) 83
BstV1I GCAGC 1 cut(s) 132
BstX2I RGATCY 1 cut(s) 158
BstYI RGATCY 1 cut(s) 158
BsuRI GGCC 2 cut(s) 100, 253
BtsI GCAGTG 1 cut(s) 247
BtsIMutI CAGTG 1 cut(s) 247
Cfr13I GGNCC 3 cut(s) 13, 98, 286
Csp6I GTAC 1 cut(s) 25
CviJI RGCY 5 cut(s) 100, 145, 253, 267, 303
CviKI_1 RGCY 5 cut(s) 100, 145, 253, 267, 303
CviQI GTAC 1 cut(s) 25
DdeI CTNAG 2 cut(s) 74, 261
DpnI GATC 1 cut(s) 160
DpnII GATC 1 cut(s) 158
DraI TTTAAA 1 cut(s) 271
DrdI GACNNNNNNGTC 1 cut(s) 224
DseDI GACNNNNNNGTC 1 cut(s) 224
EaeI YGGCCR 1 cut(s) 251
Eam1104I CTCTTC 1 cut(s) 44
EarI CTCTTC 1 cut(s) 44
Eco47I GGWCC 2 cut(s) 13, 286
Eco88I CYCGRG 1 cut(s) 95
FaiI YATR 3 cut(s) 18, 111, 173
Fnu4HI GCNGC 1 cut(s) 146
Fsp4HI GCNGC 1 cut(s) 146
GluI GCNGC 1 cut(s) 146
HaeIII GGCC 2 cut(s) 100, 253
HapII CCGG 1 cut(s) 101
HinfI GANTC 1 cut(s) 257
HpaII CCGG 1 cut(s) 101
HphI GGTGA 1 cut(s) 50
Hpy166II GTNNAC 2 cut(s) 25, 54
Hpy188I TCNGA 1 cut(s) 166
Hpy188III TCNNGA 2 cut(s) 43, 211
Hpy8I GTNNAC 2 cut(s) 25, 54
HpyCH4V TGCA 2 cut(s) 10, 148
HpyF3I CTNAG 2 cut(s) 74, 261
Kzo9I GATC 1 cut(s) 158
LpnPI CCDG 4 cut(s) 56, 74, 114, 267
Lsp1109I GCAGC 1 cut(s) 132
MaeIII GTNAC 2 cut(s) 33, 215
MalI GATC 1 cut(s) 160
MboI GATC 1 cut(s) 158
MboII GAAGA 3 cut(s) 31, 117, 153
MflI RGATCY 1 cut(s) 158
MlsI TGGCCA 1 cut(s) 253
MluCI AATT 2 cut(s) 137, 167
MluNI TGGCCA 1 cut(s) 253
MnlI CCTC 2 cut(s) 299, 302
Mox20I TGGCCA 1 cut(s) 253
MscI TGGCCA 1 cut(s) 253
MseI TTAA 3 cut(s) 128, 179, 270
Msp20I TGGCCA 1 cut(s) 253
MspI CCGG 1 cut(s) 101
MspR9I CCNGG 1 cut(s) 102
NciI CCSGG 1 cut(s) 102
NdeII GATC 1 cut(s) 158
NmuCI GTSAC 2 cut(s) 33, 215
PfeI GAWTC 1 cut(s) 257
PkrI GCNGC 1 cut(s) 147
PspPI GGNCC 3 cut(s) 13, 98, 286
PsrI GAACNNNNNNTAC 2 cut(s) 105, 137
PsuI RGATCY 1 cut(s) 158
RsaI GTAC 1 cut(s) 26
RsaNI GTAC 1 cut(s) 25
SaqAI TTAA 3 cut(s) 128, 179, 270
SatI GCNGC 1 cut(s) 146
Sau3AI GATC 1 cut(s) 158
Sau96I GGNCC 3 cut(s) 13, 98, 286
ScrFI CCNGG 1 cut(s) 102
SetI ASST 4 cut(s) 15, 75, 147, 269
SfcI CTRYAG 1 cut(s) 83
SinI GGWCC 2 cut(s) 13, 286
Sse9I AATT 2 cut(s) 137, 167
StyD4I CCNGG 1 cut(s) 100
TaqI TCGA 1 cut(s) 210
TasI AATT 2 cut(s) 137, 167
TatI WGTACW 1 cut(s) 24
TfiI GAWTC 1 cut(s) 257
Tru1I TTAA 3 cut(s) 128, 179, 270
Tru9I TTAA 3 cut(s) 128, 179, 270
TscAI CASTG 1 cut(s) 247
TseFI GTSAC 2 cut(s) 33, 215
TseI GCWGC 1 cut(s) 145
Tsp45I GTSAC 2 cut(s) 33, 215
TspRI CASTG 1 cut(s) 247
VpaK11BI GGWCC 2 cut(s) 13, 286
XapI RAATTY 2 cut(s) 137, 167
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.