pycom03g04900

Belongs to the eukaryotic ribosomal protein eS4 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
N/A
Physical Location & Seq
Reverse (-)
3821888 .. 3823396
1509 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 423 bp
ATGGCTAGAGGACTGAAGAAGCATTTGAAGAGGCTCAATGCCCCAAAGCATTGGATGCTCGACAAGCTTGGTGGTGCTTTTGCCCCCAAGCCATCATCTGGTCCCCACAAAGCCAGGGAGTGCCTTCCCCTGGTTCTTATTCTGAGGAACAGGTTGAAGTATGCACTCACATACCGTGAGGTTGTTTCAATTCTCATGCAGCGCCATATTTTGGTTGACGGAAAGGTTAGGACTGACAAGACTTACCCTTCTGGCTTCATGGATGTTGTTTCGATCCCGAAAACAAATGAAAATTTCCGTCTGCTCTTTGACACCAAGGGTCGGTTTAGGCTCCACTCAATTAGAGATGAGGAGGCAAAGTTCAAGCTCTGCAAGGTTCGATCGATCAGTGCAGTTTGGGCAGAAGGGCATCCCCTATCTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
40S_S4_C KOW RS4NT Ribosomal_S4e S4

Protein Analysis

141

Amino Acids

16.09

Weight (kDa)

10.39

Isoelectric Point (pI)

32.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RS4NT PF08071 3 - 39 5.1e-19 RS4NT (NUC023) domain
Ribosomal_S4e PF00900 95 - 131 2.3e-14 Ribosomal family S4e
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 98, 211
AclWI GGATC 1 cut(s) 268
AcsI RAATTY 1 cut(s) 292
AcuI CTGAAG 1 cut(s) 35
AfiI CCNNNNNNNGG 4 cut(s) 98, 130, 211, 321
AgsI TTSAA 4 cut(s) 28, 157, 189, 364
AjnI CCWGG 2 cut(s) 113, 129
AloI GAACNNNNNNTCC 2 cut(s) 344, 376
AluBI AGCT 2 cut(s) 67, 367
AluI AGCT 2 cut(s) 67, 367
AlwI GGATC 1 cut(s) 268
ApeKI GCWGC 1 cut(s) 199
ApoI RAATTY 1 cut(s) 292
AspLEI GCGC 1 cut(s) 204
AspS9I GGNCC 1 cut(s) 101
AvaII GGWCC 1 cut(s) 101
BbvI GCAGC 1 cut(s) 211
BccI CCATC 1 cut(s) 100
BciT130I CCWGG 2 cut(s) 115, 131
BfaI CTAG 1 cut(s) 6
BfoI RGCGCY 1 cut(s) 205
BisI GCNGC 1 cut(s) 200
BlsI GCNGC 1 cut(s) 201
Bme1390I CCNGG 2 cut(s) 115, 131
Bme18I GGWCC 1 cut(s) 101
BmgT120I GGNCC 1 cut(s) 101
BmiI GGNNCC 2 cut(s) 103, 332
BmrFI CCNGG 2 cut(s) 115, 131
BmsI GCATC 2 cut(s) 45, 418
Bsa29I ATCGAT 1 cut(s) 383
BsaJI CCNNGG 3 cut(s) 114, 129, 315
BsaXI ACNNNNNCTCC 2 cut(s) 344, 374
Bsc4I CCNNNNNNNGG 4 cut(s) 98, 130, 211, 321
BseBI CCWGG 2 cut(s) 115, 131
BseCI ATCGAT 1 cut(s) 383
BseDI CCNNGG 3 cut(s) 114, 129, 315
BseGI GGATG 3 cut(s) 60, 268, 409
BseLI CCNNNNNNNGG 4 cut(s) 98, 130, 211, 321
BseMII CTCAG 1 cut(s) 134
BseRI GAGGAG 1 cut(s) 365
BseXI GCAGC 1 cut(s) 211
BsgI GTGCAG 1 cut(s) 411
Bsh1285I CGRYCG 1 cut(s) 383
BshVI ATCGAT 1 cut(s) 383
BsiEI CGRYCG 1 cut(s) 383
BslFI GGGAC 1 cut(s) 87
BslI CCNNNNNNNGG 4 cut(s) 98, 130, 211, 321
BsmFI GGGAC 1 cut(s) 87
Bsp143I GATC 3 cut(s) 273, 380, 384
BspCNI CTCAG 1 cut(s) 135
BspDI ATCGAT 1 cut(s) 383
BspLI GGNNCC 2 cut(s) 103, 332
BspPI GGATC 1 cut(s) 268
BssECI CCNNGG 3 cut(s) 114, 129, 315
BssMI GATC 3 cut(s) 273, 380, 384
BssT1I CCWWGG 1 cut(s) 315
Bst2UI CCWGG 2 cut(s) 115, 131
Bst4CI ACNGT 1 cut(s) 176
Bst6I CTCTTC 1 cut(s) 23
BstAPI GCANNNNNTGC 1 cut(s) 55
BstDEI CTNAG 1 cut(s) 143
BstF5I GGATG 3 cut(s) 60, 268, 409
BstH2I RGCGCY 1 cut(s) 205
BstHHI GCGC 1 cut(s) 204
BstKTI GATC 3 cut(s) 276, 383, 387
BstMBI GATC 3 cut(s) 273, 380, 384
BstMCI CGRYCG 1 cut(s) 383
BstMWI GCNNNNNNNGC 3 cut(s) 55, 64, 398
BstNI CCWGG 2 cut(s) 115, 131
BstSCI CCNGG 2 cut(s) 113, 129
BstV1I GCAGC 1 cut(s) 211
BstXI CCANNNNNNTGG 1 cut(s) 51
Bsu15I ATCGAT 1 cut(s) 383
BsuTUI ATCGAT 1 cut(s) 383
BtsCI GGATG 3 cut(s) 60, 268, 409
BtsIMutI CAGTG 1 cut(s) 394
CfoI GCGC 1 cut(s) 204
Cfr13I GGNCC 1 cut(s) 101
ClaI ATCGAT 1 cut(s) 383
CspCI CAANNNNNGTGG 2 cut(s) 52, 87
CviAII CATG 2 cut(s) 196, 259
CviJI RGCY 8 cut(s) 5, 34, 67, 91, 113, 255, 331, 367
CviKI_1 RGCY 8 cut(s) 5, 34, 67, 91, 113, 255, 331, 367
DdeI CTNAG 1 cut(s) 143
DpnI GATC 3 cut(s) 275, 382, 386
DpnII GATC 3 cut(s) 273, 380, 384
Eam1104I CTCTTC 1 cut(s) 23
EarI CTCTTC 1 cut(s) 23
Eco130I CCWWGG 1 cut(s) 315
Eco47I GGWCC 1 cut(s) 101
Eco57I CTGAAG 1 cut(s) 35
EcoRII CCWGG 2 cut(s) 113, 129
EcoT14I CCWWGG 1 cut(s) 315
ErhI CCWWGG 1 cut(s) 315
FaeI CATG 2 cut(s) 199, 262
FaiI YATR 5 cut(s) 162, 172, 197, 207, 260
FaqI GGGAC 1 cut(s) 87
FatI CATG 2 cut(s) 195, 258
Fnu4HI GCNGC 1 cut(s) 200
FokI GGATG 3 cut(s) 67, 275, 396
Fsp4HI GCNGC 1 cut(s) 200
FspBI CTAG 1 cut(s) 6
GlaI GCGC 1 cut(s) 203
GluI GCNGC 1 cut(s) 200
HaeII RGCGCY 1 cut(s) 205
HhaI GCGC 1 cut(s) 204
Hin1II CATG 2 cut(s) 199, 262
Hin6I GCGC 1 cut(s) 202
HinP1I GCGC 1 cut(s) 202
HincII GTYRAC 1 cut(s) 217
HindII GTYRAC 1 cut(s) 217
HindIII AAGCTT 1 cut(s) 65
Hpy166II GTNNAC 1 cut(s) 217
Hpy188I TCNGA 1 cut(s) 144
Hpy188III TCNNGA 1 cut(s) 277
Hpy8I GTNNAC 1 cut(s) 217
HpyAV CCTTC 3 cut(s) 134, 258, 398
HpyCH4III ACNGT 1 cut(s) 176
HpyCH4V TGCA 4 cut(s) 164, 199, 372, 392
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 64, 398
HpyF3I CTNAG 1 cut(s) 143
Hsp92II CATG 2 cut(s) 199, 262
HspAI GCGC 1 cut(s) 202
Kzo9I GATC 3 cut(s) 273, 380, 384
LmnI GCTCC 1 cut(s) 336
LpnPI CCDG 7 cut(s) 84, 100, 116, 127, 136, 143, 237
Lsp1109I GCAGC 1 cut(s) 211
LweI GCATC 2 cut(s) 45, 418
MaeI CTAG 1 cut(s) 6
MalI GATC 3 cut(s) 275, 382, 386
MboI GATC 3 cut(s) 273, 380, 384
MboII GAAGA 2 cut(s) 28, 40
MluCI AATT 3 cut(s) 189, 292, 339
MnlI CCTC 5 cut(s) 24, 138, 172, 343, 346
MseI TTAA 1 cut(s) 421
MspR9I CCNGG 2 cut(s) 115, 131
MvaI CCWGG 2 cut(s) 115, 131
MwoI GCNNNNNNNGC 3 cut(s) 55, 64, 398
NdeII GATC 3 cut(s) 273, 380, 384
NlaIII CATG 2 cut(s) 199, 262
NlaIV GGNNCC 2 cut(s) 103, 332
PflMI CCANNNNNTGG 2 cut(s) 98, 211
PkrI GCNGC 1 cut(s) 201
Ple19I CGATCG 1 cut(s) 383
Psp6I CCWGG 2 cut(s) 113, 129
PspGI CCWGG 2 cut(s) 113, 129
PspN4I GGNNCC 2 cut(s) 103, 332
PspPI GGNCC 1 cut(s) 101
PvuI CGATCG 1 cut(s) 383
SaqAI TTAA 1 cut(s) 421
SatI GCNGC 1 cut(s) 200
Sau3AI GATC 3 cut(s) 273, 380, 384
Sau96I GGNCC 1 cut(s) 101
ScrFI CCNGG 2 cut(s) 115, 131
SetI ASST 6 cut(s) 69, 155, 183, 228, 369, 378
SfaNI GCATC 2 cut(s) 45, 418
SinI GGWCC 1 cut(s) 101
Sse9I AATT 3 cut(s) 189, 292, 339
SspMI CTAG 1 cut(s) 6
StyD4I CCNGG 2 cut(s) 113, 129
StyI CCWWGG 1 cut(s) 315
TaaI ACNGT 1 cut(s) 176
TaqI TCGA 4 cut(s) 60, 272, 379, 383
TasI AATT 3 cut(s) 189, 292, 339
Tru1I TTAA 1 cut(s) 421
Tru9I TTAA 1 cut(s) 421
TscAI CASTG 1 cut(s) 394
TseI GCWGC 1 cut(s) 199
TspDTI ATGAA 2 cut(s) 247, 303
TspGWI ACGGA 2 cut(s) 234, 287
TspRI CASTG 1 cut(s) 394
Van91I CCANNNNNTGG 2 cut(s) 98, 211
VpaK11BI GGWCC 1 cut(s) 101
XapI RAATTY 1 cut(s) 292
XspI CTAG 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.