pycom03g05550

Belongs to the PdxS SNZ family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
N/A
Physical Location & Seq
Reverse (-)
4410303 .. 4411250
948 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 867 bp
ATGCCATATACGAAACCACCACCAAGTCCTCCCCGTTCTCTGTCAAAGTCGGCCTCGCCCAAATGCTCCGCGGTGGCGTCATCATGGACGTTGTCAACCCCACCCAGGCCCGCATCGCCGAGGAGGCCGGCGCCTGCGCCGTCATGGCCCTGGAGAGCGTCCCTGCCGACATCCGATCCCAGGGTGGTGTCGCCCGAATGTCCGACCCCCAGCTCATCAAGGAGATCGATCAATAAGCGCTCCGTCACCATCCCAGTCATGGCCAAAGCCCGCATCGGCCATTTCGTCGAGGCCCAAATCCTCGAAGCCATCGGCGTCGACTACGTCGACGAGAGCGAGGTCCTCACTCTCGCCGACGAGGAGCACCACATCAACAAGCACAATTTTCGAGTCCCGTTCGTCTGCGGATGTCGGAATCTCGGCGAGGCGCTCCGCAGGATCCTCGAGGGCGCCGCGATGATCCGGACGAAGGGGGAAGCCGGCACCGGAAACATCGTAGAGGCCGTTCGGCACGTGCGTCACGTGATGGGGGACATCAGGGTCTTGAGGAACATGGACGACGACGAGGTGTTCGCGTACACGAAGCAGCTCGGGAGGCTGCCGGTGGTGCATTTTGCCGCCGGGGGCGTGGCGACTCCCGCAGACGCGGCCCTGATGATGCAGTTGGGGTGCGACGGGGTTTTCGTCGGGTCGGGCGTGTTCAAGAGCAGTGACCCTGCCCGGAGAGCCAGGGCTATTGTGCAGGCTTCCACCCATTACAGTGACCCAGATGTGCTGGCGGAGATCAGCTGCGGATTGGGCGAGGCAATGACCGGGATGAATCTGAACGACGAGAAGGTCGAGCGGTTCGCATTTCGGTCTGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00750 map00750
Pfam Domains
Protein Families
SOR_SNZ ThiG

Protein Analysis

289

Amino Acids

31.19

Weight (kDa)

8.77

Isoelectric Point (pI)

61.14

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
SOR_SNZ PF01680 79 - 198 2.2e-63 SOR/SNZ family
ThiG PF05690 199 - 249 1.5e-06 Thiazole biosynthesis protein ThiG
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 539, 836
AbsI CCTCGAGG 1 cut(s) 443
AccB1I GGYRCC 3 cut(s) 130, 449, 482
AccBSI CCGCTC 1 cut(s) 844
AccI GTMKAC 2 cut(s) 318, 327
AccII CGCG 4 cut(s) 71, 455, 575, 647
AccIII TCCGGA 1 cut(s) 462
AclWI GGATC 4 cut(s) 170, 433, 446, 454
AcoI YGGCCR 2 cut(s) 261, 277
AcvI CACGTG 2 cut(s) 514, 523
AcyI GRCGYC 4 cut(s) 77, 131, 315, 450
AfaI GTAC 1 cut(s) 578
AfeI AGCGCT 1 cut(s) 239
AfiI CCNNNNNNNGG 4 cut(s) 105, 180, 259, 469
AgsI TTSAA 1 cut(s) 703
AjnI CCWGG 4 cut(s) 104, 149, 179, 728
AluBI AGCT 3 cut(s) 213, 589, 789
AluI AGCT 3 cut(s) 213, 589, 789
Alw21I GWGCWC 1 cut(s) 366
AlwI GGATC 4 cut(s) 170, 433, 446, 454
Ama87I CYCGRG 2 cut(s) 443, 590
Aor13HI TCCGGA 1 cut(s) 462
Aor51HI AGCGCT 1 cut(s) 239
AoxI GGCC 9 cut(s) 51, 107, 125, 146, 261, 277, 291, 501, 648
ApeKI GCWGC 3 cut(s) 586, 598, 789
ArsI GACNNNNNNTTYG 4 cut(s) 554, 586, 665, 697
AspLEI GCGC 5 cut(s) 133, 139, 240, 430, 452
AspS9I GGNCC 5 cut(s) 108, 147, 292, 340, 649
AsuC2I CCSGG 3 cut(s) 622, 721, 814
AsuHPI GGTGA 1 cut(s) 238
AvaI CYCGRG 2 cut(s) 443, 590
AvaII GGWCC 1 cut(s) 340
BalI TGGCCA 1 cut(s) 263
BamHI GGATCC 1 cut(s) 438
BanI GGYRCC 3 cut(s) 130, 449, 482
BbrPI CACGTG 2 cut(s) 514, 523
Bbv12I GWGCWC 1 cut(s) 366
BbvI GCAGC 3 cut(s) 585, 598, 776
BccI CCATC 3 cut(s) 257, 317, 520
BceAI ACGGC 2 cut(s) 124, 488
BcgI CGANNNNNNTGC 4 cut(s) 368, 402, 424, 458
BciT130I CCWGG 4 cut(s) 106, 151, 181, 730
BcnI CCSGG 3 cut(s) 622, 721, 814
BfoI RGCGCY 4 cut(s) 134, 241, 431, 453
BglI GCCNNNNNGGC 2 cut(s) 124, 145
BisI GCNGC 6 cut(s) 453, 587, 599, 618, 648, 790
BlsI GCNGC 6 cut(s) 454, 588, 600, 619, 649, 791
Bme1390I CCNGG 7 cut(s) 106, 151, 181, 622, 721, 730, 814
Bme18I GGWCC 1 cut(s) 340
BmeT110I CYCGRG 2 cut(s) 443, 590
BmgT120I GGNCC 5 cut(s) 108, 147, 292, 340, 649
BmiI GGNNCC 4 cut(s) 132, 440, 451, 484
BmrFI CCNGG 7 cut(s) 106, 151, 181, 622, 721, 730, 814
BmrI ACTGGG 1 cut(s) 248
BmsI GCATC 3 cut(s) 122, 282, 648
BmuI ACTGGG 1 cut(s) 248
BpmI CTGGAG 1 cut(s) 172
BpuEI CTTGAG 1 cut(s) 565
BpuMI CCSGG 3 cut(s) 622, 721, 814
Bsa29I ATCGAT 1 cut(s) 227
BsaAI YACGTR 2 cut(s) 514, 523
BsaHI GRCGYC 4 cut(s) 77, 131, 315, 450
BsaJI CCNNGG 8 cut(s) 69, 104, 119, 149, 179, 180, 621, 729
BsaWI WCCGGW 2 cut(s) 462, 485
Bsc4I CCNNNNNNNGG 4 cut(s) 105, 180, 259, 469
Bse118I RCCGGY 3 cut(s) 127, 479, 601
Bse1I ACTGG 1 cut(s) 254
Bse3DI GCAATG 1 cut(s) 813
BseAI TCCGGA 1 cut(s) 462
BseBI CCWGG 4 cut(s) 106, 151, 181, 730
BseCI ATCGAT 1 cut(s) 227
BseDI CCNNGG 8 cut(s) 69, 104, 119, 149, 179, 180, 621, 729
BseGI GGATG 4 cut(s) 170, 249, 413, 822
BseLI CCNNNNNNNGG 4 cut(s) 105, 180, 259, 469
BseMI GCAATG 1 cut(s) 813
BseNI ACTGG 1 cut(s) 254
BseRI GAGGAG 2 cut(s) 136, 374
BseXI GCAGC 3 cut(s) 585, 598, 776
BseYI CCCAGC 1 cut(s) 209
BsgI GTGCAG 1 cut(s) 761
Bsh1236I CGCG 4 cut(s) 71, 455, 575, 647
BshFI GGCC 9 cut(s) 53, 109, 127, 148, 263, 279, 293, 503, 650
BshNI GGYRCC 3 cut(s) 130, 449, 482
BshVI ATCGAT 1 cut(s) 227
BsiHKAI GWGCWC 1 cut(s) 366
BsiHKCI CYCGRG 2 cut(s) 443, 590
BsiSI CCGG 8 cut(s) 128, 463, 480, 486, 602, 621, 721, 813
BslFI GGGAC 3 cut(s) 145, 377, 545
BslI CCNNNNNNNGG 4 cut(s) 105, 180, 259, 469
BsmFI GGGAC 3 cut(s) 145, 377, 545
BsnI GGCC 9 cut(s) 53, 109, 127, 148, 263, 279, 293, 503, 650
BsoBI CYCGRG 2 cut(s) 443, 590
Bsp1286I GDGCHC 1 cut(s) 366
Bsp13I TCCGGA 1 cut(s) 462
Bsp143I GATC 6 cut(s) 175, 224, 228, 438, 459, 783
BspANI GGCC 9 cut(s) 53, 109, 127, 148, 263, 279, 293, 503, 650
BspDI ATCGAT 1 cut(s) 227
BspEI TCCGGA 1 cut(s) 462
BspFNI CGCG 4 cut(s) 71, 455, 575, 647
BspLI GGNNCC 4 cut(s) 132, 440, 451, 484
BspPI GGATC 4 cut(s) 170, 433, 446, 454
BspT107I GGYRCC 3 cut(s) 130, 449, 482
BsrBI CCGCTC 1 cut(s) 844
BsrDI GCAATG 1 cut(s) 813
BsrFI RCCGGY 3 cut(s) 127, 479, 601
BsrI ACTGG 1 cut(s) 254
BssAI RCCGGY 3 cut(s) 127, 479, 601
BssECI CCNNGG 8 cut(s) 69, 104, 119, 149, 179, 180, 621, 729
BssMI GATC 6 cut(s) 175, 224, 228, 438, 459, 783
BssNI GRCGYC 4 cut(s) 77, 131, 315, 450
Bst2UI CCWGG 4 cut(s) 106, 151, 181, 730
Bst4CI ACNGT 1 cut(s) 761
BstACI GRCGYC 4 cut(s) 77, 131, 315, 450
BstBAI YACGTR 2 cut(s) 514, 523
BstC8I GCNNGC 7 cut(s) 111, 129, 135, 271, 481, 744, 777
BstDSI CCRYGG 1 cut(s) 69
BstF5I GGATG 4 cut(s) 170, 249, 413, 822
BstFNI CGCG 4 cut(s) 71, 455, 575, 647
BstH2I RGCGCY 4 cut(s) 134, 241, 431, 453
BstHHI GCGC 5 cut(s) 133, 139, 240, 430, 452
BstKTI GATC 6 cut(s) 178, 227, 231, 441, 462, 786
BstMBI GATC 6 cut(s) 175, 224, 228, 438, 459, 783
BstMWI GCNNNNNNNGC 9 cut(s) 115, 124, 145, 595, 607, 638, 647, 725, 798
BstNI CCWGG 4 cut(s) 106, 151, 181, 730
BstSCI CCNGG 7 cut(s) 104, 149, 179, 620, 719, 728, 812
BstUI CGCG 4 cut(s) 71, 455, 575, 647
BstV1I GCAGC 3 cut(s) 585, 598, 776
BstX2I RGATCY 1 cut(s) 438
BstYI RGATCY 1 cut(s) 438
Bsu15I ATCGAT 1 cut(s) 227
BsuRI GGCC 9 cut(s) 53, 109, 127, 148, 263, 279, 293, 503, 650
BsuTUI ATCGAT 1 cut(s) 227
BtgI CCRYGG 1 cut(s) 69
BtgZI GCGATG 2 cut(s) 99, 470
BtsCI GGATG 4 cut(s) 170, 249, 413, 822
BtsI GCAGTG 1 cut(s) 715
BtsIMutI CAGTG 2 cut(s) 715, 766
Cac8I GCNNGC 7 cut(s) 111, 129, 135, 271, 481, 744, 777
CfoI GCGC 5 cut(s) 133, 139, 240, 430, 452
Cfr10I RCCGGY 3 cut(s) 127, 479, 601
Cfr13I GGNCC 5 cut(s) 108, 147, 292, 340, 649
Cfr42I CCGCGG 1 cut(s) 72
ClaI ATCGAT 1 cut(s) 227
CseI GACGC 5 cut(s) 66, 147, 304, 506, 653
Csp6I GTAC 1 cut(s) 577
CviAII CATG 4 cut(s) 84, 144, 259, 553
CviQI GTAC 1 cut(s) 577
DinI GGCGCC 2 cut(s) 132, 451
DpnI GATC 6 cut(s) 177, 226, 230, 440, 461, 785
DpnII GATC 6 cut(s) 175, 224, 228, 438, 459, 783
DrdI GACNNNNNNGTC 2 cut(s) 539, 836
DseDI GACNNNNNNGTC 2 cut(s) 539, 836
EaeI YGGCCR 2 cut(s) 261, 277
EciI GGCGGA 1 cut(s) 794
Eco47I GGWCC 1 cut(s) 340
Eco47III AGCGCT 1 cut(s) 239
Eco72I CACGTG 2 cut(s) 514, 523
Eco88I CYCGRG 2 cut(s) 443, 590
EcoO109I RGGNCCY 1 cut(s) 340
EcoRII CCWGG 4 cut(s) 104, 149, 179, 728
EgeI GGCGCC 2 cut(s) 132, 451
EheI GGCGCC 2 cut(s) 132, 451
FaeI CATG 4 cut(s) 87, 147, 262, 556
FaiI YATR 6 cut(s) 7, 9, 85, 145, 260, 554
FaqI GGGAC 3 cut(s) 145, 377, 545
FatI CATG 4 cut(s) 83, 143, 258, 552
FauI CCCGC 3 cut(s) 118, 278, 646
FblI GTMKAC 2 cut(s) 318, 327
Fnu4HI GCNGC 6 cut(s) 453, 587, 599, 618, 648, 790
FokI GGATG 4 cut(s) 157, 236, 420, 829
Fsp4HI GCNGC 6 cut(s) 453, 587, 599, 618, 648, 790
GlaI GCGC 5 cut(s) 132, 138, 239, 429, 451
GluI GCNGC 6 cut(s) 453, 587, 599, 618, 648, 790
GsaI CCCAGC 1 cut(s) 213
GsuI CTGGAG 1 cut(s) 172
HaeII RGCGCY 4 cut(s) 134, 241, 431, 453
HaeIII GGCC 9 cut(s) 53, 109, 127, 148, 263, 279, 293, 503, 650
HapII CCGG 8 cut(s) 128, 463, 480, 486, 602, 621, 721, 813
HgaI GACGC 5 cut(s) 66, 147, 304, 506, 653
HhaI GCGC 5 cut(s) 133, 139, 240, 430, 452
Hin1I GRCGYC 4 cut(s) 77, 131, 315, 450
Hin1II CATG 4 cut(s) 87, 147, 262, 556
Hin6I GCGC 5 cut(s) 131, 137, 238, 428, 450
HinP1I GCGC 5 cut(s) 131, 137, 238, 428, 450
HincII GTYRAC 3 cut(s) 96, 319, 328
HindII GTYRAC 3 cut(s) 96, 319, 328
HinfI GANTC 4 cut(s) 390, 415, 634, 820
HpaII CCGG 8 cut(s) 128, 463, 480, 486, 602, 621, 721, 813
HphI GGTGA 1 cut(s) 238
Hpy166II GTNNAC 4 cut(s) 96, 319, 328, 579
Hpy188I TCNGA 5 cut(s) 175, 204, 414, 825, 862
Hpy188III TCNNGA 4 cut(s) 463, 544, 592, 703
Hpy8I GTNNAC 4 cut(s) 96, 319, 328, 579
HpyAV CCTTC 2 cut(s) 463, 829
HpyCH4III ACNGT 1 cut(s) 761
HpyCH4IV ACGT 4 cut(s) 89, 324, 513, 522
HpyCH4V TGCA 3 cut(s) 610, 661, 742
HpyF10VI GCNNNNNNNGC 9 cut(s) 115, 124, 145, 595, 607, 638, 647, 725, 798
HpySE526I ACGT 4 cut(s) 89, 324, 513, 522
Hsp92I GRCGYC 4 cut(s) 77, 131, 315, 450
Hsp92II CATG 4 cut(s) 87, 147, 262, 556
HspAI GCGC 5 cut(s) 131, 137, 238, 428, 450
KasI GGCGCC 2 cut(s) 130, 449
Kpn2I TCCGGA 1 cut(s) 462
KroI GCCGGC 2 cut(s) 127, 479
KroNI GCCGGC 2 cut(s) 129, 481
KspI CCGCGG 1 cut(s) 72
Kzo9I GATC 6 cut(s) 175, 224, 228, 438, 459, 783
LmnI GCTCC 4 cut(s) 71, 245, 361, 435
Lsp1109I GCAGC 3 cut(s) 585, 598, 776
LweI GCATC 3 cut(s) 122, 282, 648
MaeII ACGT 4 cut(s) 89, 324, 513, 522
MaeIII GTNAC 4 cut(s) 244, 518, 710, 761
MalI GATC 6 cut(s) 177, 226, 230, 440, 461, 785
MbiI CCGCTC 1 cut(s) 844
MboI GATC 6 cut(s) 175, 224, 228, 438, 459, 783
MflI RGATCY 1 cut(s) 438
MhlI GDGCHC 1 cut(s) 366
MlsI TGGCCA 1 cut(s) 263
MluCI AATT 1 cut(s) 382
MluNI TGGCCA 1 cut(s) 263
Mly113I GGCGCC 2 cut(s) 131, 450
MlyI GAGTC 2 cut(s) 399, 628
MmeI TCCRAC 2 cut(s) 227, 392
Mox20I TGGCCA 1 cut(s) 263
MroI TCCGGA 1 cut(s) 462
MroNI GCCGGC 2 cut(s) 127, 479
MscI TGGCCA 1 cut(s) 263
MslI CAYNNNNRTG 1 cut(s) 759
Msp20I TGGCCA 1 cut(s) 263
MspA1I CMGCKG 2 cut(s) 71, 789
MspI CCGG 8 cut(s) 128, 463, 480, 486, 602, 621, 721, 813
MspR9I CCNGG 7 cut(s) 106, 151, 181, 622, 721, 730, 814
MvaI CCWGG 4 cut(s) 106, 151, 181, 730
MvnI CGCG 4 cut(s) 71, 455, 575, 647
MwoI GCNNNNNNNGC 9 cut(s) 115, 124, 145, 595, 607, 638, 647, 725, 798
NaeI GCCGGC 2 cut(s) 129, 481
NarI GGCGCC 2 cut(s) 131, 450
NciI CCSGG 3 cut(s) 622, 721, 814
NdeII GATC 6 cut(s) 175, 224, 228, 438, 459, 783
NgoMIV GCCGGC 2 cut(s) 127, 479
NlaIII CATG 4 cut(s) 87, 147, 262, 556
NlaIV GGNNCC 4 cut(s) 132, 440, 451, 484
NmeAIII GCCGAG 2 cut(s) 144, 399
NmuCI GTSAC 4 cut(s) 244, 518, 710, 761
PaeR7I CTCGAG 1 cut(s) 443
PasI CCCWGGG 1 cut(s) 180
PcsI WCGNNNNNNNCGW 9 cut(s) 282, 324, 333, 501, 514, 519, 681, 693, 837
PdiI GCCGGC 2 cut(s) 129, 481
PfeI GAWTC 2 cut(s) 415, 820
PflFI GACNNNGTC 2 cut(s) 91, 323
PkrI GCNGC 6 cut(s) 454, 588, 600, 619, 649, 791
PleI GAGTC 2 cut(s) 398, 628
PluTI GGCGCC 2 cut(s) 134, 453
PmaCI CACGTG 2 cut(s) 514, 523
PmlI CACGTG 2 cut(s) 514, 523
PpsI GAGTC 2 cut(s) 398, 628
Ppu21I YACGTR 2 cut(s) 514, 523
PpuMI RGGWCCY 1 cut(s) 340
Psp5II RGGWCCY 1 cut(s) 340
Psp6I CCWGG 4 cut(s) 104, 149, 179, 728
PspCI CACGTG 2 cut(s) 514, 523
PspFI CCCAGC 1 cut(s) 209
PspGI CCWGG 4 cut(s) 104, 149, 179, 728
PspN4I GGNNCC 4 cut(s) 132, 440, 451, 484
PspPI GGNCC 5 cut(s) 108, 147, 292, 340, 649
PspPPI RGGWCCY 1 cut(s) 340
PspXI VCTCGAGB 1 cut(s) 443
PsrI GAACNNNNNNTAC 2 cut(s) 489, 521
PsuI RGATCY 1 cut(s) 438
PsyI GACNNNGTC 2 cut(s) 91, 323
PvuII CAGCTG 1 cut(s) 789
RsaI GTAC 1 cut(s) 578
RsaNI GTAC 1 cut(s) 577
RseI CAYNNNNRTG 1 cut(s) 759
SacII CCGCGG 1 cut(s) 72
SalI GTCGAC 2 cut(s) 317, 326
SatI GCNGC 6 cut(s) 453, 587, 599, 618, 648, 790
Sau3AI GATC 6 cut(s) 175, 224, 228, 438, 459, 783
Sau96I GGNCC 5 cut(s) 108, 147, 292, 340, 649
SchI GAGTC 2 cut(s) 399, 628
ScrFI CCNGG 7 cut(s) 106, 151, 181, 622, 721, 730, 814
SduI GDGCHC 1 cut(s) 366
SfaNI GCATC 3 cut(s) 122, 282, 648
SfoI GGCGCC 2 cut(s) 132, 451
Sfr274I CTCGAG 1 cut(s) 443
Sfr303I CCGCGG 1 cut(s) 72
SgrBI CCGCGG 1 cut(s) 72
SgrDI CGTCGACG 1 cut(s) 326
SinI GGWCC 1 cut(s) 340
SlaI CTCGAG 1 cut(s) 443
SmiMI CAYNNNNRTG 1 cut(s) 759
SmlI CTYRAG 2 cut(s) 443, 544
SmoI CTYRAG 2 cut(s) 443, 544
Sse9I AATT 1 cut(s) 382
SspDI GGCGCC 2 cut(s) 130, 449
StyD4I CCNGG 7 cut(s) 104, 149, 179, 620, 719, 728, 812
TaaI ACNGT 1 cut(s) 761
TaiI ACGT 4 cut(s) 92, 327, 516, 525
TaqI TCGA 8 cut(s) 227, 288, 303, 318, 327, 388, 444, 840
TaqII GACCGA 1 cut(s) 846
TasI AATT 1 cut(s) 382
TauI GCSGC 3 cut(s) 455, 620, 650
TfiI GAWTC 2 cut(s) 415, 820
TscAI CASTG 2 cut(s) 715, 766
TseFI GTSAC 4 cut(s) 244, 518, 710, 761
TseI GCWGC 3 cut(s) 586, 598, 789
Tsp45I GTSAC 4 cut(s) 244, 518, 710, 761
TspDTI ATGAA 1 cut(s) 833
TspGWI ACGGA 1 cut(s) 232
TspRI CASTG 2 cut(s) 715, 766
Tth111I GACNNNGTC 2 cut(s) 91, 323
VpaK11BI GGWCC 1 cut(s) 340
XcmI CCANNNNNNNNNTGG 1 cut(s) 256
XhoI CTCGAG 1 cut(s) 443
XmiI GTMKAC 2 cut(s) 318, 327
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.