pycom03g06100

Acyl-coenzyme A thioesterase 13-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
N/A
Physical Location & Seq
Forward (+)
4849016 .. 4849228
213 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 213 bp
ATGAATATAAGCCCATCCGAACCCGGCCCGCTCAACTTCTGCAAAGATGGATCCGAGTCAAAGTGGTGCAAAAGTCAGCATGGAGAAGGCGAACAAATCCTTGGAACTGACCCACGACGAGTTAGAGGCGCCGGCGCCGGGCCGAGCTTCTACGAGCCTTACGCTCTTCGTGGCTTCCGAGTCGACCGAGTCGAATCCGGACTCGTCGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
4HBT

Protein Analysis

71

Amino Acids

7.62

Weight (kDa)

6.55

Isoelectric Point (pI)

54.43

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 128, 134
AccBSI CCGCTC 1 cut(s) 31
AccI GTMKAC 1 cut(s) 183
AccIII TCCGGA 1 cut(s) 197
AciI CCGC 1 cut(s) 29
AclWI GGATC 2 cut(s) 45, 58
AcyI GRCGYC 2 cut(s) 129, 135
AfiI CCNNNNNNNGG 1 cut(s) 138
AhdI GACNNNNNGTC 1 cut(s) 206
AjuI GAANNNNNNNTTGG 2 cut(s) 84, 116
AluBI AGCT 1 cut(s) 147
AluI AGCT 1 cut(s) 147
AlwI GGATC 2 cut(s) 45, 58
Aor13HI TCCGGA 1 cut(s) 197
AoxI GGCC 2 cut(s) 25, 140
AspLEI GCGC 2 cut(s) 131, 137
AspS9I GGNCC 2 cut(s) 26, 140
AsuC2I CCSGG 2 cut(s) 24, 139
BamHI GGATCC 1 cut(s) 50
BanI GGYRCC 2 cut(s) 128, 134
BccI CCATC 2 cut(s) 22, 41
BcnI CCSGG 2 cut(s) 24, 139
BfoI RGCGCY 2 cut(s) 132, 138
Bme1390I CCNGG 2 cut(s) 24, 139
BmeRI GACNNNNNGTC 1 cut(s) 206
BmgT120I GGNCC 2 cut(s) 26, 140
BmiI GGNNCC 3 cut(s) 52, 130, 136
BmrFI CCNGG 2 cut(s) 24, 139
BpuMI CCSGG 2 cut(s) 24, 139
BsaHI GRCGYC 2 cut(s) 129, 135
BsaJI CCNNGG 1 cut(s) 100
BsaWI WCCGGW 1 cut(s) 197
Bsc4I CCNNNNNNNGG 1 cut(s) 138
Bse118I RCCGGY 1 cut(s) 131
BseAI TCCGGA 1 cut(s) 197
BseDI CCNNGG 1 cut(s) 100
BseGI GGATG 1 cut(s) 14
BseLI CCNNNNNNNGG 1 cut(s) 138
Bsh1285I CGRYCG 1 cut(s) 187
BshFI GGCC 2 cut(s) 27, 142
BshNI GGYRCC 2 cut(s) 128, 134
BsiEI CGRYCG 1 cut(s) 187
BsiSI CCGG 4 cut(s) 24, 132, 138, 198
BslI CCNNNNNNNGG 1 cut(s) 138
BsnI GGCC 2 cut(s) 27, 142
Bsp13I TCCGGA 1 cut(s) 197
Bsp143I GATC 1 cut(s) 50
BspACI CCGC 1 cut(s) 29
BspANI GGCC 2 cut(s) 27, 142
BspEI TCCGGA 1 cut(s) 197
BspLI GGNNCC 3 cut(s) 52, 130, 136
BspPI GGATC 2 cut(s) 45, 58
BspQI GCTCTTC 1 cut(s) 171
BspT107I GGYRCC 2 cut(s) 128, 134
BsrBI CCGCTC 1 cut(s) 31
BsrFI RCCGGY 1 cut(s) 131
BssAI RCCGGY 1 cut(s) 131
BssECI CCNNGG 1 cut(s) 100
BssMI GATC 1 cut(s) 50
BssNI GRCGYC 2 cut(s) 129, 135
BssT1I CCWWGG 1 cut(s) 100
Bst6I CTCTTC 1 cut(s) 171
BstACI GRCGYC 2 cut(s) 129, 135
BstC8I GCNNGC 2 cut(s) 29, 133
BstF5I GGATG 1 cut(s) 14
BstH2I RGCGCY 2 cut(s) 132, 138
BstHHI GCGC 2 cut(s) 131, 137
BstKTI GATC 1 cut(s) 53
BstMBI GATC 1 cut(s) 50
BstMCI CGRYCG 1 cut(s) 187
BstSCI CCNGG 2 cut(s) 22, 137
BstX2I RGATCY 1 cut(s) 50
BstYI RGATCY 1 cut(s) 50
BsuRI GGCC 2 cut(s) 27, 142
BtsCI GGATG 1 cut(s) 14
Cac8I GCNNGC 2 cut(s) 29, 133
CfoI GCGC 2 cut(s) 131, 137
Cfr10I RCCGGY 1 cut(s) 131
Cfr13I GGNCC 2 cut(s) 26, 140
CviAII CATG 1 cut(s) 80
CviJI RGCY 6 cut(s) 12, 27, 142, 147, 157, 174
CviKI_1 RGCY 6 cut(s) 12, 27, 142, 147, 157, 174
DinI GGCGCC 2 cut(s) 130, 136
DpnI GATC 1 cut(s) 52
DpnII GATC 1 cut(s) 50
DriI GACNNNNNGTC 1 cut(s) 206
Eam1104I CTCTTC 1 cut(s) 171
Eam1105I GACNNNNNGTC 1 cut(s) 206
EarI CTCTTC 1 cut(s) 171
Eco130I CCWWGG 1 cut(s) 100
EcoT14I CCWWGG 1 cut(s) 100
EgeI GGCGCC 2 cut(s) 130, 136
EheI GGCGCC 2 cut(s) 130, 136
ErhI CCWWGG 1 cut(s) 100
FaeI CATG 1 cut(s) 83
FaiI YATR 2 cut(s) 8, 81
FatI CATG 1 cut(s) 79
FauI CCCGC 1 cut(s) 36
FblI GTMKAC 1 cut(s) 183
GlaI GCGC 2 cut(s) 130, 136
HaeII RGCGCY 2 cut(s) 132, 138
HaeIII GGCC 2 cut(s) 27, 142
HapII CCGG 4 cut(s) 24, 132, 138, 198
HhaI GCGC 2 cut(s) 131, 137
Hin1I GRCGYC 2 cut(s) 129, 135
Hin1II CATG 1 cut(s) 83
Hin6I GCGC 2 cut(s) 129, 135
HinP1I GCGC 2 cut(s) 129, 135
HincII GTYRAC 1 cut(s) 184
HindII GTYRAC 1 cut(s) 184
HinfI GANTC 5 cut(s) 56, 180, 189, 194, 201
HpaII CCGG 4 cut(s) 24, 132, 138, 198
Hpy166II GTNNAC 1 cut(s) 184
Hpy188I TCNGA 4 cut(s) 19, 55, 179, 212
Hpy188III TCNNGA 1 cut(s) 198
Hpy8I GTNNAC 1 cut(s) 184
Hpy99I CGWCG 2 cut(s) 120, 209
HpyAV CCTTC 1 cut(s) 80
HpyCH4V TGCA 2 cut(s) 42, 69
Hsp92I GRCGYC 2 cut(s) 129, 135
Hsp92II CATG 1 cut(s) 83
HspAI GCGC 2 cut(s) 129, 135
KasI GGCGCC 2 cut(s) 128, 134
Kpn2I TCCGGA 1 cut(s) 197
KroI GCCGGC 1 cut(s) 131
KroNI GCCGGC 1 cut(s) 133
Kzo9I GATC 1 cut(s) 50
LguI GCTCTTC 1 cut(s) 171
LpnPI CCDG 3 cut(s) 37, 145, 151
MalI GATC 1 cut(s) 52
MbiI CCGCTC 1 cut(s) 31
MboI GATC 1 cut(s) 50
MboII GAAGA 1 cut(s) 158
MflI RGATCY 1 cut(s) 50
Mly113I GGCGCC 2 cut(s) 129, 135
MlyI GAGTC 4 cut(s) 65, 189, 195, 198
MnlI CCTC 1 cut(s) 119
MreI CGCCGGCG 1 cut(s) 131
MroI TCCGGA 1 cut(s) 197
MroNI GCCGGC 1 cut(s) 131
MspI CCGG 4 cut(s) 24, 132, 138, 198
MspR9I CCNGG 2 cut(s) 24, 139
NaeI GCCGGC 1 cut(s) 133
NarI GGCGCC 2 cut(s) 129, 135
NciI CCSGG 2 cut(s) 24, 139
NdeII GATC 1 cut(s) 50
NgoMIV GCCGGC 1 cut(s) 131
NlaIII CATG 1 cut(s) 83
NlaIV GGNNCC 3 cut(s) 52, 130, 136
NmeAIII GCCGAG 1 cut(s) 168
PciSI GCTCTTC 1 cut(s) 171
PcsI WCGNNNNNNNCGW 2 cut(s) 175, 189
PdiI GCCGGC 1 cut(s) 133
PfeI GAWTC 1 cut(s) 194
PflFI GACNNNGTC 1 cut(s) 188
PleI GAGTC 4 cut(s) 64, 188, 195, 197
PluTI GGCGCC 2 cut(s) 132, 138
PpsI GAGTC 4 cut(s) 64, 188, 195, 197
PspN4I GGNNCC 3 cut(s) 52, 130, 136
PspPI GGNCC 2 cut(s) 26, 140
PsuI RGATCY 1 cut(s) 50
PsyI GACNNNGTC 1 cut(s) 188
SalI GTCGAC 1 cut(s) 182
SapI GCTCTTC 1 cut(s) 171
Sau3AI GATC 1 cut(s) 50
Sau96I GGNCC 2 cut(s) 26, 140
SchI GAGTC 4 cut(s) 65, 189, 195, 198
ScrFI CCNGG 2 cut(s) 24, 139
SetI ASST 1 cut(s) 149
SfoI GGCGCC 2 cut(s) 130, 136
SgrAI CRCCGGYG 1 cut(s) 131
SsiI CCGC 1 cut(s) 29
SspDI GGCGCC 2 cut(s) 128, 134
StyD4I CCNGG 2 cut(s) 22, 137
StyI CCWWGG 1 cut(s) 100
TaqI TCGA 2 cut(s) 183, 192
TaqII GACCGA 1 cut(s) 201
TfiI GAWTC 1 cut(s) 194
TspDTI ATGAA 1 cut(s) 17
Tth111I GACNNNGTC 1 cut(s) 188
XmiI GTMKAC 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.