pycom03g06890

pumilio homolog

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
N/A
Physical Location & Seq
Reverse (-)
5427848 .. 5429328
1481 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1008 bp
ATGAATCCAGCGTTGGCGTCCATGGTGGCTAGCCAACTTGGCGCTGGTAATCTTCCACCCTTGTTTGAATCAGCTATGCGATCCCCTGGAATGGATTCAAGAGTGCTTGGAGGCCGTATGTCCTCTGGACCAAACCTAGCAGCTGCTGCAAATGAATCACTTAATCTTGGTGGCTTGGGGAGTCCGATTGCAGGGAGCGGTCTTCAGGCTCCCTTTGTTGATCCTATGTATCTTCAGTACTTGAGGACATCTGAGTATGCTGCAGCACAGCTTGGTGCTCTTAATGATCCTTCTGTGGATAGGAACTACTTGGGTAATTCGTACATGAATTTACTTGAACTTCAAAAGGCGTATCTTGGAGCCTTGCTGTCACCACAGAAGTCACAGTATGGTGTTCCTATGGTTGGCAAGTCTGGTGGTTCTAATCATCAGGGGTATTATGGAAATCCTGCATTTGGTCTAGGCATGTCTTATCCTGGAAGTCCTCCTGTTATTCCTAACTCTCCAGTTGGACCAGCTACTCCTATGAGGCACAATGAACTCAATATGTGTTATCCCTCTGGGATGAGGAATTTAGCTCCCTGGCACCTGGATGGTGGACGTAACATAGATGAAAGTTTTGCTTCTTCACTCTTAGAAGAATTTAAGAGCAACAAAGCCAAGTCTTTTGAACTTTCAGAGATCGGCGGACATGTTGTGGAGTTCAGTGCGGATCAGTATGGGAGTCGTTTTATTCAACAAAAACTTGAAACTGCCACTACAGAAGAGAAAAACATGGTTTATCAAGAAATCATGCCTCAAGCTCTTGCTCTAATGACTGATGTGTTTGGTAATTATGTGATTCAAAAGTTTTTTGAGCATGGACTTCCACCTCAGAGAAGGGAATTGGCCAACAAGCTTTTCAGTCATGTACTGACACTTAGCCTTCAAATGTATGGTTGTCGGGTGATCCAGAAGGCAATAGAAGTTGTTGATCGATCTTCTGGACCAAAAGATCAAAATGGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0003730 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0044424 GO:0044444 GO:0044464 GO:0097159 GO:1901363
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
NABP PUF

Protein Analysis

336

Amino Acids

36.18

Weight (kDa)

6.09

Isoelectric Point (pI)

55.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
NABP PF07990 1 - 226 1e-98 Nucleic acid binding protein NABP
PUF PF00806 231 - 265 2.3e-11 Pumilio-family RNA binding repeat
PUF PF00806 269 - 300 1.9e-08 Pumilio-family RNA binding repeat
PUF PF00806 303 - 325 6.3e-07 Pumilio-family RNA binding repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 585
AccBSI CCGCTC 1 cut(s) 198
AciI CCGC 3 cut(s) 198, 687, 710
AclWI GGATC 5 cut(s) 75, 215, 281, 720, 943
AcoI YGGCCR 1 cut(s) 888
AcsI RAATTY 3 cut(s) 328, 571, 641
AcuI CTGAAG 2 cut(s) 188, 218
AcyI GRCGYC 1 cut(s) 17
AfaI GTAC 3 cut(s) 239, 323, 912
AfiI CCNNNNNNNGG 4 cut(s) 91, 191, 404, 455
AflIII ACRYGT 1 cut(s) 691
AgsI TTSAA 9 cut(s) 68, 99, 338, 344, 671, 737, 749, 845, 929
AjnI CCWGG 4 cut(s) 85, 475, 581, 588
AjuI GAANNNNNNNTTGG 3 cut(s) 28, 884, 916
AluBI AGCT 7 cut(s) 74, 143, 271, 518, 578, 803, 898
AluI AGCT 7 cut(s) 74, 143, 271, 518, 578, 803, 898
Alw21I GWGCWC 1 cut(s) 280
AlwI GGATC 5 cut(s) 75, 215, 281, 720, 943
AlwNI CAGNNNCTG 1 cut(s) 146
AoxI GGCC 2 cut(s) 112, 888
ApeKI GCWGC 5 cut(s) 140, 143, 146, 260, 263
ApoI RAATTY 3 cut(s) 328, 571, 641
Asp700I GAANNNNTTC 1 cut(s) 94
AspLEI GCGC 1 cut(s) 44
AspS9I GGNCC 3 cut(s) 128, 512, 986
AsuHPI GGTGA 2 cut(s) 363, 958
AsuNHI GCTAGC 1 cut(s) 29
AvaII GGWCC 3 cut(s) 128, 512, 986
BalI TGGCCA 1 cut(s) 890
BanI GGYRCC 1 cut(s) 585
BbsI GAAGAC 1 cut(s) 194
Bbv12I GWGCWC 1 cut(s) 280
BbvI GCAGC 5 cut(s) 130, 133, 152, 247, 275
BccI CCATC 1 cut(s) 587
BceAI ACGGC 1 cut(s) 99
BciT130I CCWGG 4 cut(s) 87, 477, 583, 590
BfaI CTAG 3 cut(s) 30, 137, 461
BfmI CTRYAG 2 cut(s) 261, 759
BfoI RGCGCY 1 cut(s) 45
BglI GCCNNNNNGGC 1 cut(s) 39
BisI GCNGC 5 cut(s) 141, 144, 147, 261, 264
BlsI GCNGC 5 cut(s) 142, 145, 148, 262, 265
BmcAI AGTACT 1 cut(s) 239
Bme1390I CCNGG 4 cut(s) 87, 477, 583, 590
Bme18I GGWCC 3 cut(s) 128, 512, 986
BmgT120I GGNCC 3 cut(s) 128, 512, 986
BmiI GGNNCC 3 cut(s) 210, 361, 587
BmrFI CCNGG 4 cut(s) 87, 477, 583, 590
BmtI GCTAGC 1 cut(s) 33
BpiI GAAGAC 1 cut(s) 194
BpmI CTGGAG 1 cut(s) 489
BpuEI CTTGAG 2 cut(s) 262, 783
Bsa29I ATCGAT 1 cut(s) 976
BsaHI GRCGYC 1 cut(s) 17
BsaJI CCNNGG 3 cut(s) 21, 85, 581
Bsc4I CCNNNNNNNGG 4 cut(s) 91, 191, 404, 455
Bse1I ACTGG 1 cut(s) 506
BseBI CCWGG 4 cut(s) 87, 477, 583, 590
BseCI ATCGAT 1 cut(s) 976
BseDI CCNNGG 3 cut(s) 21, 85, 581
BseGI GGATG 2 cut(s) 570, 598
BseLI CCNNNNNNNGG 4 cut(s) 91, 191, 404, 455
BseMII CTCAG 2 cut(s) 243, 887
BseNI ACTGG 1 cut(s) 506
BseXI GCAGC 5 cut(s) 130, 133, 152, 247, 275
BshFI GGCC 2 cut(s) 114, 890
BshNI GGYRCC 1 cut(s) 585
BshVI ATCGAT 1 cut(s) 976
BsiHKAI GWGCWC 1 cut(s) 280
BslI CCNNNNNNNGG 4 cut(s) 91, 191, 404, 455
BsnI GGCC 2 cut(s) 114, 890
Bsp1286I GDGCHC 1 cut(s) 280
Bsp143I GATC 9 cut(s) 80, 220, 286, 681, 712, 948, 973, 977, 994
Bsp19I CCATGG 1 cut(s) 21
BspACI CCGC 3 cut(s) 198, 687, 710
BspANI GGCC 2 cut(s) 114, 890
BspCNI CTCAG 2 cut(s) 244, 886
BspDI ATCGAT 1 cut(s) 976
BspLI GGNNCC 3 cut(s) 210, 361, 587
BspMAI CTGCAG 1 cut(s) 265
BspOI GCTAGC 1 cut(s) 33
BspPI GGATC 5 cut(s) 75, 215, 281, 720, 943
BspT107I GGYRCC 1 cut(s) 585
BsrBI CCGCTC 1 cut(s) 198
BsrI ACTGG 1 cut(s) 506
BssECI CCNNGG 3 cut(s) 21, 85, 581
BssMI GATC 9 cut(s) 80, 220, 286, 681, 712, 948, 973, 977, 994
BssNI GRCGYC 1 cut(s) 17
BssT1I CCWWGG 1 cut(s) 21
Bst2UI CCWGG 4 cut(s) 87, 477, 583, 590
Bst4CI ACNGT 1 cut(s) 387
Bst6I CTCTTC 1 cut(s) 759
BstACI GRCGYC 1 cut(s) 17
BstAPI GCANNNNNTGC 1 cut(s) 146
BstC8I GCNNGC 1 cut(s) 31
BstDEI CTNAG 4 cut(s) 252, 634, 873, 920
BstDSI CCRYGG 1 cut(s) 21
BstF5I GGATG 2 cut(s) 570, 598
BstH2I RGCGCY 1 cut(s) 45
BstHHI GCGC 1 cut(s) 44
BstKTI GATC 9 cut(s) 83, 223, 289, 684, 715, 951, 976, 980, 997
BstMBI GATC 9 cut(s) 80, 220, 286, 681, 712, 948, 973, 977, 994
BstMWI GCNNNNNNNGC 2 cut(s) 39, 146
BstNI CCWGG 4 cut(s) 87, 477, 583, 590
BstNSI RCATGY 2 cut(s) 469, 695
BstSCI CCNGG 4 cut(s) 85, 475, 581, 588
BstSFI CTRYAG 2 cut(s) 261, 759
BstV1I GCAGC 5 cut(s) 130, 133, 152, 247, 275
BstV2I GAAGAC 1 cut(s) 194
Bsu15I ATCGAT 1 cut(s) 976
BsuRI GGCC 2 cut(s) 114, 890
BsuTUI ATCGAT 1 cut(s) 976
BtgI CCRYGG 1 cut(s) 21
BtsCI GGATG 2 cut(s) 570, 598
BtsIMutI CAGTG 1 cut(s) 712
Cac8I GCNNGC 1 cut(s) 31
CaiI CAGNNNCTG 1 cut(s) 146
CfoI GCGC 1 cut(s) 44
Cfr13I GGNCC 3 cut(s) 128, 512, 986
ClaI ATCGAT 1 cut(s) 976
CseI GACGC 1 cut(s) 6
Csp6I GTAC 3 cut(s) 238, 322, 911
CspCI CAANNNNNGTGG 2 cut(s) 397, 432
CviAII CATG 8 cut(s) 22, 325, 466, 692, 775, 793, 860, 908
CviQI GTAC 3 cut(s) 238, 322, 911
DdeI CTNAG 4 cut(s) 252, 634, 873, 920
DpnI GATC 9 cut(s) 82, 222, 288, 683, 714, 950, 975, 979, 996
DpnII GATC 9 cut(s) 80, 220, 286, 681, 712, 948, 973, 977, 994
EaeI YGGCCR 1 cut(s) 888
Eam1104I CTCTTC 1 cut(s) 759
EarI CTCTTC 1 cut(s) 759
EciI GGCGGA 1 cut(s) 702
Eco130I CCWWGG 1 cut(s) 21
Eco47I GGWCC 3 cut(s) 128, 512, 986
Eco57I CTGAAG 2 cut(s) 188, 218
EcoRII CCWGG 4 cut(s) 85, 475, 581, 588
EcoT14I CCWWGG 1 cut(s) 21
ErhI CCWWGG 1 cut(s) 21
FaeI CATG 8 cut(s) 25, 328, 469, 695, 778, 796, 863, 911
FalI AAGNNNNNCTT 2 cut(s) 607, 639
FatI CATG 8 cut(s) 21, 324, 465, 691, 774, 792, 859, 907
Fnu4HI GCNGC 5 cut(s) 141, 144, 147, 261, 264
FokI GGATG 2 cut(s) 577, 605
Fsp4HI GCNGC 5 cut(s) 141, 144, 147, 261, 264
FspBI CTAG 3 cut(s) 30, 137, 461
GlaI GCGC 1 cut(s) 43
GluI GCNGC 5 cut(s) 141, 144, 147, 261, 264
GsuI CTGGAG 1 cut(s) 489
HaeII RGCGCY 1 cut(s) 45
HaeIII GGCC 2 cut(s) 114, 890
HgaI GACGC 1 cut(s) 6
HhaI GCGC 1 cut(s) 44
Hin1I GRCGYC 1 cut(s) 17
Hin1II CATG 8 cut(s) 25, 328, 469, 695, 778, 796, 863, 911
Hin6I GCGC 1 cut(s) 42
HinP1I GCGC 1 cut(s) 42
HindIII AAGCTT 1 cut(s) 896
HinfI GANTC 7 cut(s) 4, 68, 95, 155, 181, 724, 841
HphI GGTGA 2 cut(s) 363, 958
Hpy166II GTNNAC 1 cut(s) 599
Hpy188I TCNGA 4 cut(s) 186, 253, 679, 876
Hpy188III TCNNGA 5 cut(s) 99, 126, 785, 952, 984
Hpy8I GTNNAC 1 cut(s) 599
HpyAV CCTTC 4 cut(s) 300, 873, 935, 949
HpyCH4III ACNGT 1 cut(s) 387
HpyCH4IV ACGT 1 cut(s) 601
HpyCH4V TGCA 4 cut(s) 149, 191, 263, 452
HpyF10VI GCNNNNNNNGC 2 cut(s) 39, 146
HpyF3I CTNAG 4 cut(s) 252, 634, 873, 920
HpySE526I ACGT 1 cut(s) 601
Hsp92I GRCGYC 1 cut(s) 17
Hsp92II CATG 8 cut(s) 25, 328, 469, 695, 778, 796, 863, 911
HspAI GCGC 1 cut(s) 42
Kzo9I GATC 9 cut(s) 80, 220, 286, 681, 712, 948, 973, 977, 994
LmnI GCTCC 4 cut(s) 195, 214, 359, 583
Lsp1109I GCAGC 5 cut(s) 130, 133, 152, 247, 275
MaeI CTAG 3 cut(s) 30, 137, 461
MaeII ACGT 1 cut(s) 601
MaeIII GTNAC 3 cut(s) 369, 381, 602
MalI GATC 9 cut(s) 82, 222, 288, 683, 714, 950, 975, 979, 996
MbiI CCGCTC 1 cut(s) 198
MboI GATC 9 cut(s) 80, 220, 286, 681, 712, 948, 973, 977, 994
MboII GAAGA 7 cut(s) 44, 194, 224, 618, 650, 776, 972
MhlI GDGCHC 1 cut(s) 280
MlsI TGGCCA 1 cut(s) 890
MluCI AATT 6 cut(s) 316, 328, 571, 641, 832, 884
MluNI TGGCCA 1 cut(s) 890
MlyI GAGTC 2 cut(s) 190, 733
MmeI TCCRAC 1 cut(s) 490
MnlI CCTC 9 cut(s) 104, 133, 237, 495, 522, 561, 568, 807, 882
Mox20I TGGCCA 1 cut(s) 890
MroXI GAANNNNTTC 1 cut(s) 94
MscI TGGCCA 1 cut(s) 890
MseI TTAA 3 cut(s) 162, 282, 645
MslI CAYNNNNRTG 1 cut(s) 591
Msp20I TGGCCA 1 cut(s) 890
MspA1I CMGCKG 1 cut(s) 143
MspR9I CCNGG 4 cut(s) 87, 477, 583, 590
MvaI CCWGG 4 cut(s) 87, 477, 583, 590
MwoI GCNNNNNNNGC 2 cut(s) 39, 146
NcoI CCATGG 1 cut(s) 21
NdeII GATC 9 cut(s) 80, 220, 286, 681, 712, 948, 973, 977, 994
NheI GCTAGC 1 cut(s) 29
NlaIII CATG 8 cut(s) 25, 328, 469, 695, 778, 796, 863, 911
NlaIV GGNNCC 3 cut(s) 210, 361, 587
NmuCI GTSAC 2 cut(s) 369, 381
NspI RCATGY 2 cut(s) 469, 695
PciI ACATGT 1 cut(s) 691
PdmI GAANNNNTTC 1 cut(s) 94
PfeI GAWTC 5 cut(s) 4, 68, 95, 155, 841
PfoI TCCNGGA 1 cut(s) 475
PkrI GCNGC 5 cut(s) 142, 145, 148, 262, 265
PleI GAGTC 2 cut(s) 189, 732
PpsI GAGTC 2 cut(s) 189, 732
PscI ACATGT 1 cut(s) 691
Psp6I CCWGG 4 cut(s) 85, 475, 581, 588
PspGI CCWGG 4 cut(s) 85, 475, 581, 588
PspN4I GGNNCC 3 cut(s) 210, 361, 587
PspPI GGNCC 3 cut(s) 128, 512, 986
PstI CTGCAG 1 cut(s) 265
PstNI CAGNNNCTG 1 cut(s) 146
PvuII CAGCTG 1 cut(s) 143
RsaI GTAC 3 cut(s) 239, 323, 912
RsaNI GTAC 3 cut(s) 238, 322, 911
RseI CAYNNNNRTG 1 cut(s) 591
SaqAI TTAA 3 cut(s) 162, 282, 645
SatI GCNGC 5 cut(s) 141, 144, 147, 261, 264
Sau3AI GATC 9 cut(s) 80, 220, 286, 681, 712, 948, 973, 977, 994
Sau96I GGNCC 3 cut(s) 128, 512, 986
ScaI AGTACT 1 cut(s) 239
SchI GAGTC 2 cut(s) 190, 733
ScrFI CCNGG 4 cut(s) 87, 477, 583, 590
SduI GDGCHC 1 cut(s) 280
SfcI CTRYAG 2 cut(s) 261, 759
SinI GGWCC 3 cut(s) 128, 512, 986
SmiMI CAYNNNNRTG 1 cut(s) 591
SmlI CTYRAG 2 cut(s) 241, 798
SmoI CTYRAG 2 cut(s) 241, 798
Sse9I AATT 6 cut(s) 316, 328, 571, 641, 832, 884
SsiI CCGC 3 cut(s) 198, 687, 710
SspMI CTAG 3 cut(s) 30, 137, 461
StyD4I CCNGG 4 cut(s) 85, 475, 581, 588
StyI CCWWGG 1 cut(s) 21
TaaI ACNGT 1 cut(s) 387
TaiI ACGT 1 cut(s) 604
TaqI TCGA 1 cut(s) 976
TasI AATT 6 cut(s) 316, 328, 571, 641, 832, 884
TatI WGTACW 2 cut(s) 237, 910
TfiI GAWTC 5 cut(s) 4, 68, 95, 155, 841
Tru1I TTAA 3 cut(s) 162, 282, 645
Tru9I TTAA 3 cut(s) 162, 282, 645
TscAI CASTG 1 cut(s) 712
TseFI GTSAC 2 cut(s) 369, 381
TseI GCWGC 5 cut(s) 140, 143, 146, 260, 263
Tsp45I GTSAC 2 cut(s) 369, 381
TspDTI ATGAA 5 cut(s) 17, 168, 341, 552, 627
TspRI CASTG 1 cut(s) 712
VpaK11BI GGWCC 3 cut(s) 128, 512, 986
XapI RAATTY 3 cut(s) 328, 571, 641
XceI RCATGY 2 cut(s) 469, 695
XcmI CCANNNNNNNNNTGG 1 cut(s) 41
XmnI GAANNNNTTC 1 cut(s) 94
XspI CTAG 3 cut(s) 30, 137, 461
ZrmI AGTACT 1 cut(s) 239
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.