pycom03g07720

Domain of unknown function (DUF4378)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
N/A
Physical Location & Seq
Reverse (-)
6121724 .. 6122413
690 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 438 bp
ATGTTCCATTCCGAGCAAACCCTATTACTGCGGGGACACTTCCGCTTAATATGTCTAACACCAGATTCTTCTCTTGACCTACAAGGCAGGCACATCATAGACTCTTTACCAGCATCCTCCAAAACTATAAGCACTGAAATTGAACGTAAGAGTTTAAACAATCACGCCTCGCGCTTTGTAGTAGACAAAGCGCATTATCCTGAATTTAAACAATCACTCCTCGACTTTAAACATTCTACCAACCTCTGGATCTTACAATGTTCAAGGAATTGGATAATTGTTTTGCATGAAATCGGAACATACGAAGACCAGGTTACATCGGGCAATTGGGATCACCGCCTCCTGTTTGATTTATTCCCAAGGACCTTCTCTTCCAATGGCTGCATTCGTCCAATGCCGAAGGGGCAACATCTTCTCGATGTGTCTGGACAAGAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF3741 DUF4378

Protein Analysis

146

Amino Acids

16.76

Weight (kDa)

6.75

Isoelectric Point (pI)

51.02

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 246
AccI GTMKAC 1 cut(s) 183
AccII CGCG 1 cut(s) 172
AciI CCGC 3 cut(s) 31, 43, 337
AclWI GGATC 2 cut(s) 257, 339
AcsI RAATTY 1 cut(s) 203
AfiI CCNNNNNNNGG 1 cut(s) 246
AgsI TTSAA 2 cut(s) 143, 264
AjnI CCWGG 1 cut(s) 309
AlwI GGATC 2 cut(s) 257, 339
ApeKI GCWGC 1 cut(s) 381
ApoI RAATTY 1 cut(s) 203
AspLEI GCGC 2 cut(s) 174, 193
AspS9I GGNCC 1 cut(s) 363
AsuHPI GGTGA 1 cut(s) 326
AvaII GGWCC 1 cut(s) 363
BbsI GAAGAC 1 cut(s) 312
BbvI GCAGC 1 cut(s) 368
BciT130I CCWGG 1 cut(s) 311
BglI GCCNNNNNGGC 1 cut(s) 403
BisI GCNGC 1 cut(s) 382
BlsI GCNGC 1 cut(s) 383
Bme1390I CCNGG 1 cut(s) 311
Bme18I GGWCC 1 cut(s) 363
BmgT120I GGNCC 1 cut(s) 363
BmrFI CCNGG 1 cut(s) 311
BmsI GCATC 1 cut(s) 122
BpiI GAAGAC 1 cut(s) 312
BsaJI CCNNGG 1 cut(s) 359
BsaXI ACNNNNNCTCC 2 cut(s) 201, 231
Bsc4I CCNNNNNNNGG 1 cut(s) 246
BseBI CCWGG 1 cut(s) 311
BseDI CCNNGG 1 cut(s) 359
BseGI GGATG 1 cut(s) 113
BseLI CCNNNNNNNGG 1 cut(s) 246
BseRI GAGGAG 1 cut(s) 209
BseXI GCAGC 1 cut(s) 368
Bsh1236I CGCG 1 cut(s) 172
BslFI GGGAC 1 cut(s) 48
BslI CCNNNNNNNGG 1 cut(s) 246
BsmFI GGGAC 1 cut(s) 48
BsmI GAATGC 1 cut(s) 384
Bsp143I GATC 2 cut(s) 249, 331
BspACI CCGC 3 cut(s) 31, 43, 337
BspFNI CGCG 1 cut(s) 172
BspPI GGATC 2 cut(s) 257, 339
BssECI CCNNGG 1 cut(s) 359
BssMI GATC 2 cut(s) 249, 331
BssT1I CCWWGG 1 cut(s) 359
Bst2UI CCWGG 1 cut(s) 311
Bst6I CTCTTC 1 cut(s) 376
BstC8I GCNNGC 1 cut(s) 89
BstF5I GGATG 1 cut(s) 113
BstFNI CGCG 1 cut(s) 172
BstHHI GCGC 2 cut(s) 174, 193
BstKTI GATC 2 cut(s) 252, 334
BstMBI GATC 2 cut(s) 249, 331
BstMWI GCNNNNNNNGC 1 cut(s) 403
BstNI CCWGG 1 cut(s) 311
BstSCI CCNGG 1 cut(s) 309
BstUI CGCG 1 cut(s) 172
BstV1I GCAGC 1 cut(s) 368
BstV2I GAAGAC 1 cut(s) 312
BstX2I RGATCY 1 cut(s) 249
BstYI RGATCY 1 cut(s) 249
BtsCI GGATG 1 cut(s) 113
BtsIMutI CAGTG 1 cut(s) 132
Cac8I GCNNGC 1 cut(s) 89
CfoI GCGC 2 cut(s) 174, 193
Cfr13I GGNCC 1 cut(s) 363
CsiI ACCWGGT 1 cut(s) 309
CviAII CATG 1 cut(s) 287
CviJI RGCY 1 cut(s) 381
CviKI_1 RGCY 1 cut(s) 381
DpnI GATC 2 cut(s) 251, 333
DpnII GATC 2 cut(s) 249, 331
DraI TTTAAA 3 cut(s) 156, 208, 229
Eam1104I CTCTTC 1 cut(s) 376
EarI CTCTTC 1 cut(s) 376
Eco130I CCWWGG 1 cut(s) 359
Eco47I GGWCC 1 cut(s) 363
EcoO109I RGGNCCY 1 cut(s) 363
EcoRII CCWGG 1 cut(s) 309
EcoT14I CCWWGG 1 cut(s) 359
ErhI CCWWGG 1 cut(s) 359
FaeI CATG 1 cut(s) 290
FaiI YATR 5 cut(s) 52, 98, 128, 288, 301
FaqI GGGAC 1 cut(s) 48
FatI CATG 1 cut(s) 286
FauI CCCGC 1 cut(s) 24
FblI GTMKAC 1 cut(s) 183
Fnu4HI GCNGC 1 cut(s) 382
FokI GGATG 1 cut(s) 100
Fsp4HI GCNGC 1 cut(s) 382
GlaI GCGC 2 cut(s) 173, 192
GluI GCNGC 1 cut(s) 382
HhaI GCGC 2 cut(s) 174, 193
Hin1II CATG 1 cut(s) 290
Hin6I GCGC 2 cut(s) 172, 191
HinP1I GCGC 2 cut(s) 172, 191
HinfI GANTC 2 cut(s) 65, 101
HphI GGTGA 1 cut(s) 326
Hpy166II GTNNAC 1 cut(s) 184
Hpy188I TCNGA 2 cut(s) 13, 296
Hpy188III TCNNGA 5 cut(s) 74, 200, 247, 416, 426
Hpy8I GTNNAC 1 cut(s) 184
HpyAV CCTTC 2 cut(s) 376, 394
HpyCH4IV ACGT 1 cut(s) 145
HpyCH4V TGCA 2 cut(s) 286, 384
HpyF10VI GCNNNNNNNGC 1 cut(s) 403
HpySE526I ACGT 1 cut(s) 145
Hsp92II CATG 1 cut(s) 290
HspAI GCGC 2 cut(s) 172, 191
Kzo9I GATC 2 cut(s) 249, 331
LpnPI CCDG 9 cut(s) 73, 75, 123, 213, 232, 296, 323, 356, 411
Lsp1109I GCAGC 1 cut(s) 368
LweI GCATC 1 cut(s) 122
MabI ACCWGGT 1 cut(s) 309
MaeII ACGT 1 cut(s) 145
MaeIII GTNAC 1 cut(s) 313
MalI GATC 2 cut(s) 251, 333
MboI GATC 2 cut(s) 249, 331
MboII GAAGA 4 cut(s) 60, 317, 363, 404
MfeI CAATTG 1 cut(s) 325
MflI RGATCY 1 cut(s) 249
MluCI AATT 5 cut(s) 138, 203, 268, 276, 325
MlyI GAGTC 1 cut(s) 95
MnlI CCTC 5 cut(s) 127, 178, 230, 254, 350
MseI TTAA 4 cut(s) 47, 155, 207, 228
MspR9I CCNGG 1 cut(s) 311
MssI GTTTAAAC 1 cut(s) 156
MunI CAATTG 1 cut(s) 325
Mva1269I GAATGC 1 cut(s) 384
MvaI CCWGG 1 cut(s) 311
MvnI CGCG 1 cut(s) 172
MwoI GCNNNNNNNGC 1 cut(s) 403
NdeII GATC 2 cut(s) 249, 331
NlaIII CATG 1 cut(s) 290
PcsI WCGNNNNNNNCGW 1 cut(s) 300
PctI GAATGC 1 cut(s) 384
PfeI GAWTC 1 cut(s) 65
PflMI CCANNNNNTGG 1 cut(s) 246
PkrI GCNGC 1 cut(s) 383
PleI GAGTC 1 cut(s) 95
PmeI GTTTAAAC 1 cut(s) 156
PpsI GAGTC 1 cut(s) 95
PpuMI RGGWCCY 1 cut(s) 363
Psp5II RGGWCCY 1 cut(s) 363
Psp6I CCWGG 1 cut(s) 309
PspGI CCWGG 1 cut(s) 309
PspPI GGNCC 1 cut(s) 363
PspPPI RGGWCCY 1 cut(s) 363
PsuI RGATCY 1 cut(s) 249
SaqAI TTAA 4 cut(s) 47, 155, 207, 228
SatI GCNGC 1 cut(s) 382
Sau3AI GATC 2 cut(s) 249, 331
Sau96I GGNCC 1 cut(s) 363
SchI GAGTC 1 cut(s) 95
ScrFI CCNGG 1 cut(s) 311
SetI ASST 5 cut(s) 81, 148, 246, 315, 368
SexAI ACCWGGT 1 cut(s) 309
SfaNI GCATC 1 cut(s) 122
SinI GGWCC 1 cut(s) 363
Sse9I AATT 5 cut(s) 138, 203, 268, 276, 325
SsiI CCGC 3 cut(s) 31, 43, 337
StyD4I CCNGG 1 cut(s) 309
StyI CCWWGG 1 cut(s) 359
TaiI ACGT 1 cut(s) 148
TaqI TCGA 2 cut(s) 222, 417
TasI AATT 5 cut(s) 138, 203, 268, 276, 325
TfiI GAWTC 1 cut(s) 65
Tru1I TTAA 4 cut(s) 47, 155, 207, 228
Tru9I TTAA 4 cut(s) 47, 155, 207, 228
TscAI CASTG 1 cut(s) 139
TseI GCWGC 1 cut(s) 381
TspDTI ATGAA 1 cut(s) 303
TspRI CASTG 1 cut(s) 139
Van91I CCANNNNNTGG 1 cut(s) 246
VpaK11BI GGWCC 1 cut(s) 363
XapI RAATTY 1 cut(s) 203
XmiI GTMKAC 1 cut(s) 183
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.