pycom03g12020

Est1 DNA/RNA binding domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
N/A
Physical Location & Seq
Forward (+)
12363676 .. 12364084
409 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 195 bp
ATGATTCCACAGATGGATAACATGTCTGCTCCTTCATCAAGGGAGCGTGCCCAACGCCTGTATGACAAGATTCTTGAGTTGGAGAATAGGCGTCGGAGGTCTGCCCAAGCACGAATTCCATCAGATCCCAATGCCTGGCAACAGATGCGTGAGAATTATGAAGCAATAATTCTTGAGGATCATCGCTTTTTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000184 GO:0000212 GO:0000226 GO:0000280 GO:0000723 GO:0000932 GO:0000956 GO:0003674 GO:0003676 GO:0003677 GO:0003723 GO:0003824 GO:0004518 GO:0004540 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005697 GO:0005737 GO:0006139 GO:0006259 GO:0006278 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0006996 GO:0007004 GO:0007010 GO:0007017 GO:0007049 GO:0007051 GO:0008104 GO:0008150 GO:0008152 GO:0009056 GO:0009057 GO:0009058 GO:0009059 GO:0009892 GO:0009894 GO:0009987 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0010833 GO:0015031 GO:0015833 GO:0015931 GO:0016043 GO:0016070 GO:0016071 GO:0016787 GO:0016788 GO:0018130 GO:0019219 GO:0019222 GO:0019438 GO:0019439 GO:0022402 GO:0022414 GO:0022607 GO:0031323 GO:0031329 GO:0031503 GO:0032200 GO:0032991 GO:0033036 GO:0034613 GO:0034641 GO:0034645 GO:0034654 GO:0034655 GO:0035770 GO:0036464 GO:0042162 GO:0042592 GO:0042886 GO:0043021 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043487 GO:0043565 GO:0044085 GO:0044237 GO:0044238 GO:0044248 GO:0044249 GO:0044260 GO:0044265 GO:0044270 GO:0044271 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048285 GO:0048519 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051028 GO:0051168 GO:0051169 GO:0051171 GO:0051179 GO:0051225 GO:0051234 GO:0051236 GO:0051252 GO:0051276 GO:0051321 GO:0051641 GO:0051649 GO:0060249 GO:0060255 GO:0065007 GO:0065008 GO:0070034 GO:0070727 GO:0070925 GO:0071166 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0071897 GO:0080090 GO:0090304 GO:0090305 GO:0090306 GO:0090501 GO:0097159 GO:0140013 GO:0140098 GO:1901360 GO:1901361 GO:1901362 GO:1901363 GO:1901575 GO:1901576 GO:1903046 GO:1990904
KEGG Pathways
Metabolic & Signaling
ko03015 map03015
Pfam Domains
Protein Families
EST1 EST1_DNA_bind

Protein Analysis

65

Amino Acids

7.78

Weight (kDa)

8.27

Isoelectric Point (pI)

78.31

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 135
AclWI GGATC 2 cut(s) 119, 186
AcsI RAATTY 1 cut(s) 114
AcyI GRCGYC 1 cut(s) 91
AfiI CCNNNNNNNGG 1 cut(s) 135
AflIII ACRYGT 1 cut(s) 21
AjnI CCWGG 1 cut(s) 134
AjuI GAANNNNNNNTTGG 2 cut(s) 99, 131
AlwI GGATC 2 cut(s) 119, 186
ApoI RAATTY 1 cut(s) 114
BaeGI GKGCMC 1 cut(s) 52
BccI CCATC 2 cut(s) 7, 127
BciT130I CCWGG 1 cut(s) 136
Bme1390I CCNGG 1 cut(s) 136
BmrFI CCNGG 1 cut(s) 136
BmsI GCATC 1 cut(s) 135
BpuEI CTTGAG 2 cut(s) 95, 194
BsaHI GRCGYC 1 cut(s) 91
Bsc4I CCNNNNNNNGG 1 cut(s) 135
BseBI CCWGG 1 cut(s) 136
BseLI CCNNNNNNNGG 1 cut(s) 135
BseSI GKGCMC 1 cut(s) 52
BslI CCNNNNNNNGG 1 cut(s) 135
Bsp1286I GDGCHC 1 cut(s) 52
Bsp143I GATC 2 cut(s) 124, 178
BspPI GGATC 2 cut(s) 119, 186
BssMI GATC 2 cut(s) 124, 178
BssNI GRCGYC 1 cut(s) 91
Bst2UI CCWGG 1 cut(s) 136
BstACI GRCGYC 1 cut(s) 91
BstAPI GCANNNNNTGC 1 cut(s) 145
BstC8I GCNNGC 1 cut(s) 48
BstKTI GATC 2 cut(s) 127, 181
BstMBI GATC 2 cut(s) 124, 178
BstMWI GCNNNNNNNGC 1 cut(s) 145
BstNI CCWGG 1 cut(s) 136
BstNSI RCATGY 1 cut(s) 25
BstSCI CCNGG 1 cut(s) 134
BstSLI GKGCMC 1 cut(s) 52
BstX2I RGATCY 1 cut(s) 124
BstYI RGATCY 1 cut(s) 124
BtgZI GCGATG 1 cut(s) 167
Cac8I GCNNGC 1 cut(s) 48
CseI GACGC 1 cut(s) 80
CviAII CATG 1 cut(s) 22
DpnI GATC 2 cut(s) 126, 180
DpnII GATC 2 cut(s) 124, 178
EcoRI GAATTC 1 cut(s) 114
EcoRII CCWGG 1 cut(s) 134
FaeI CATG 1 cut(s) 25
FaiI YATR 3 cut(s) 23, 63, 159
FatI CATG 1 cut(s) 21
HgaI GACGC 1 cut(s) 80
Hin1I GRCGYC 1 cut(s) 91
Hin1II CATG 1 cut(s) 25
HinfI GANTC 2 cut(s) 4, 70
Hpy188I TCNGA 3 cut(s) 96, 124, 194
Hpy188III TCNNGA 2 cut(s) 74, 173
Hpy99I CGWCG 1 cut(s) 96
HpyAV CCTTC 1 cut(s) 42
HpyF10VI GCNNNNNNNGC 1 cut(s) 145
Hsp92I GRCGYC 1 cut(s) 91
Hsp92II CATG 1 cut(s) 25
Kzo9I GATC 2 cut(s) 124, 178
LmnI GCTCC 2 cut(s) 34, 43
LpnPI CCDG 3 cut(s) 71, 121, 148
LweI GCATC 1 cut(s) 135
MalI GATC 2 cut(s) 126, 180
MboI GATC 2 cut(s) 124, 178
MflI RGATCY 1 cut(s) 124
MhlI GDGCHC 1 cut(s) 52
MluCI AATT 3 cut(s) 114, 154, 168
MmeI TCCRAC 2 cut(s) 60, 74
MnlI CCTC 2 cut(s) 90, 169
MspR9I CCNGG 1 cut(s) 136
MvaI CCWGG 1 cut(s) 136
MwoI GCNNNNNNNGC 1 cut(s) 145
NdeII GATC 2 cut(s) 124, 178
NlaIII CATG 1 cut(s) 25
NspI RCATGY 1 cut(s) 25
PciI ACATGT 1 cut(s) 21
PfeI GAWTC 2 cut(s) 4, 70
PflMI CCANNNNNTGG 1 cut(s) 135
PscI ACATGT 1 cut(s) 21
Psp6I CCWGG 1 cut(s) 134
PspGI CCWGG 1 cut(s) 134
PsuI RGATCY 1 cut(s) 124
Sau3AI GATC 2 cut(s) 124, 178
ScrFI CCNGG 1 cut(s) 136
SduI GDGCHC 1 cut(s) 52
SetI ASST 1 cut(s) 101
SfaNI GCATC 1 cut(s) 135
SmlI CTYRAG 2 cut(s) 74, 173
SmoI CTYRAG 2 cut(s) 74, 173
Sse9I AATT 3 cut(s) 114, 154, 168
StyD4I CCNGG 1 cut(s) 134
TasI AATT 3 cut(s) 114, 154, 168
TfiI GAWTC 2 cut(s) 4, 70
TspDTI ATGAA 2 cut(s) 24, 174
Van91I CCANNNNNTGG 1 cut(s) 135
XapI RAATTY 1 cut(s) 114
XceI RCATGY 1 cut(s) 25
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.