pycom03g12600

Nuclear cap-binding protein subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0028905
Physical Location & Seq
Forward (+)
13259540 .. 13272887
13348 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1128 bp
ATGAGCAGTTGGAAGAGTCTTCTTCTTCGAATTGGCGAGAAATCTCCCGACTATGGCACCTCCTCCGACGTCAAAGAACACATTGAAACTTGCTATGGCGTGCTTCGCCGAGAATTGGAGCACTCTCCTGACGACGTTTCGCAATACCTTATCCAGTGTGCTGAACAGTTGCCTCACAAGAGTCCTCTATATGGGACAGTGATTGGCTTGTTAAATTTGGAGAACGAGGAATTTATTAGGAAAGTAGTTAAGAACACTCAAACTAATTTCCAGGATGCTTTAGATTCCGGAAATTGTAACAAGATTCGTATTTTGATGAGGTTCCTGACTGTTATGATGTGCAGTAAAGTTATCCATCCCAGTTCTCTTGTGGTTGTGTTTGAAACATTATTATCATCAGCTGCAACAACAGTTGATGAGGAGAAAGGCAATCCGTCCTGGCAATCGCGAGCTGACTTTTACATAACATGCATTTTATCTTGTCTCCCTTGGGGAGGAGCGGAACTCATTGAGCAAGTTCCTGAGGAAATTGAGAGAGTTATGGTCGGTGTTGAAGCTTATTTGAGCATAAGAAAACGTGCTTCTGACACTGGGTTGTCCTTTTTTGAGGATGATGATGAGAATGAGAGAGAACCTAGCGATAAGGATTTCTTGGAAGATTTGTGGGGCCGAATACAAGTTTTATCTAGCAATGGATGGAAACTTGATAGTGTTCCGAGACCTCACCTATCATTTGAAGCACAGCTAGTTGCTGGAAAGTCTCATGAGTTTGGACCCATCGGCTGTCCTGACCAACCTGCTATGCCTTCAACAATGTCTAATATAACTTATGGTAAACAAAAGCATGATGCAGAGTTTATCTATCCGCAAAGGATACGAAGGCTGAATATATTTCCTGAAAGTAAAACTGAGGACGTGCAACCCATGGATCGTTTGAGATATCATTGTGGAAGAGTATCTGCTAGATGTGCTATTTTACTTAAATGGCTGCTAGTCGAAAGGAATGTGCTTCATATATGGCTGGCCTACCTGTGCCCTTTAGATATGAGTATCTTATGGCAGAGACAATTTTCTCCCAGTTGCTTATGCTACCACAACCCCCTTTCAGGACAATATATTACACGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000184 GO:0000339 GO:0000375 GO:0000377 GO:0000394 GO:0000398 GO:0000956 GO:0001101 GO:0003006 GO:0003674 GO:0003676 GO:0003723 GO:0003729 GO:0005488 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005845 GO:0005846 GO:0006139 GO:0006396 GO:0006397 GO:0006401 GO:0006402 GO:0006403 GO:0006405 GO:0006406 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0008380 GO:0009056 GO:0009057 GO:0009314 GO:0009416 GO:0009628 GO:0009648 GO:0009719 GO:0009725 GO:0009737 GO:0009791 GO:0009892 GO:0009987 GO:0010033 GO:0010228 GO:0010467 GO:0010468 GO:0010605 GO:0010608 GO:0010629 GO:0014070 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016071 GO:0016441 GO:0016458 GO:0019222 GO:0019439 GO:0022414 GO:0031047 GO:0031050 GO:0031053 GO:0031503 GO:0032501 GO:0032502 GO:0032991 GO:0033036 GO:0033993 GO:0034470 GO:0034518 GO:0034613 GO:0034641 GO:0034655 GO:0034660 GO:0035194 GO:0035195 GO:0035196 GO:0040029 GO:0042221 GO:0042886 GO:0043170 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0043331 GO:0044237 GO:0044238 GO:0044248 GO:0044260 GO:0044265 GO:0044270 GO:0044424 GO:0044464 GO:0045184 GO:0046483 GO:0046700 GO:0046907 GO:0048519 GO:0048571 GO:0048573 GO:0048574 GO:0048608 GO:0048731 GO:0048856 GO:0050657 GO:0050658 GO:0050789 GO:0050896 GO:0051028 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051641 GO:0051649 GO:0051716 GO:0060255 GO:0061458 GO:0065007 GO:0070727 GO:0070887 GO:0070918 GO:0071166 GO:0071310 GO:0071359 GO:0071407 GO:0071426 GO:0071427 GO:0071702 GO:0071704 GO:0071705 GO:0090304 GO:0097159 GO:0097305 GO:1901360 GO:1901361 GO:1901363 GO:1901575 GO:1901698 GO:1901699 GO:1901700
KEGG Pathways
Metabolic & Signaling
ko03013 ko03015 ko03040 map03013 map03015 map03040
Pfam Domains
Protein Families
MIF4G MIF4G_like MIF4G_like_2

Protein Analysis

376

Amino Acids

42.91

Weight (kDa)

5.55

Isoelectric Point (pI)

56.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIF4G PF02854 20 - 224 4e-31 MIF4G domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0028905)

Species Orthologous Gene IDs
malus_domestica MD11G1196400.v1.1
pyrus_communis pycom03g12600

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 72
Acc36I ACCTGC 1 cut(s) 805
AccB1I GGYRCC 1 cut(s) 56
AccBSI CCGCTC 1 cut(s) 500
AccII CGCG 1 cut(s) 448
AccIII TCCGGA 1 cut(s) 287
AciI CCGC 2 cut(s) 500, 866
AclWI GGATC 1 cut(s) 936
AcsI RAATTY 2 cut(s) 214, 230
AcyI GRCGYC 1 cut(s) 69
AfiI CCNNNNNNNGG 5 cut(s) 53, 115, 191, 494, 1106
AgsI TTSAA 5 cut(s) 86, 383, 554, 737, 810
AjiI CACGTC 1 cut(s) 916
AjnI CCWGG 2 cut(s) 270, 437
AluBI AGCT 4 cut(s) 401, 452, 557, 745
AluI AGCT 4 cut(s) 401, 452, 557, 745
Alw21I GWGCWC 1 cut(s) 123
Alw26I GTCTC 4 cut(s) 488, 712, 765, 1057
AlwI GGATC 1 cut(s) 936
Aor13HI TCCGGA 1 cut(s) 287
AoxI GGCC 2 cut(s) 667, 1023
ApeKI GCWGC 2 cut(s) 401, 988
ApoI RAATTY 2 cut(s) 214, 230
AspS9I GGNCC 2 cut(s) 667, 773
AsuHPI GGTGA 1 cut(s) 716
AsuII TTCGAA 1 cut(s) 28
AvaII GGWCC 1 cut(s) 773
AxyI CCTNAGG 1 cut(s) 522
BaeGI GKGCMC 1 cut(s) 1037
BanI GGYRCC 1 cut(s) 56
BbsI GAAGAC 1 cut(s) 11
Bbv12I GWGCWC 1 cut(s) 123
BbvI GCAGC 2 cut(s) 388, 975
BccI CCATC 3 cut(s) 363, 690, 785
BciT130I CCWGG 2 cut(s) 272, 439
BciVI GTATCC 1 cut(s) 867
BcoDI GTCTC 4 cut(s) 488, 712, 765, 1057
BfaI CTAG 6 cut(s) 636, 687, 746, 963, 992, 1126
BfuAI ACCTGC 1 cut(s) 805
BfuI GTATCC 1 cut(s) 867
BisI GCNGC 2 cut(s) 402, 989
BlsI GCNGC 2 cut(s) 403, 990
Bme1390I CCNGG 2 cut(s) 272, 439
Bme18I GGWCC 1 cut(s) 773
BmgBI CACGTC 1 cut(s) 916
BmgT120I GGNCC 2 cut(s) 667, 773
BmiI GGNNCC 4 cut(s) 58, 323, 668, 775
BmrFI CCNGG 2 cut(s) 272, 439
BmrI ACTGGG 3 cut(s) 354, 600, 1071
BmsI GCATC 2 cut(s) 265, 838
BmuI ACTGGG 3 cut(s) 354, 600, 1071
BpiI GAAGAC 1 cut(s) 11
BplI GAGNNNNNCTC 2 cut(s) 489, 521
Bpu14I TTCGAA 1 cut(s) 28
BsaHI GRCGYC 1 cut(s) 69
BsaI GGTCTC 1 cut(s) 712
BsaJI CCNNGG 2 cut(s) 488, 924
BsaWI WCCGGW 1 cut(s) 287
Bsc4I CCNNNNNNNGG 5 cut(s) 53, 115, 191, 494, 1106
Bse1I ACTGG 4 cut(s) 154, 360, 595, 1077
Bse21I CCTNAGG 1 cut(s) 522
Bse3DI GCAATG 1 cut(s) 697
BseAI TCCGGA 1 cut(s) 287
BseBI CCWGG 2 cut(s) 272, 439
BseDI CCNNGG 2 cut(s) 488, 924
BseGI GGATG 4 cut(s) 280, 355, 616, 701
BseLI CCNNNNNNNGG 5 cut(s) 53, 115, 191, 494, 1106
BseMI GCAATG 1 cut(s) 697
BseMII CTCAG 2 cut(s) 513, 900
BseNI ACTGG 4 cut(s) 154, 360, 595, 1077
BseRI GAGGAG 3 cut(s) 52, 434, 510
BseSI GKGCMC 1 cut(s) 1037
BseXI GCAGC 2 cut(s) 388, 975
BsgI GTGCAG 1 cut(s) 361
Bsh1236I CGCG 1 cut(s) 448
BshFI GGCC 2 cut(s) 669, 1025
BshNI GGYRCC 1 cut(s) 56
BsiHKAI GWGCWC 1 cut(s) 123
BsiSI CCGG 1 cut(s) 288
BslFI GGGAC 1 cut(s) 208
BslI CCNNNNNNNGG 5 cut(s) 53, 115, 191, 494, 1106
BsmAI GTCTC 4 cut(s) 488, 712, 765, 1057
BsmFI GGGAC 1 cut(s) 208
BsnI GGCC 2 cut(s) 669, 1025
Bso31I GGTCTC 1 cut(s) 712
Bsp119I TTCGAA 1 cut(s) 28
Bsp1286I GDGCHC 2 cut(s) 123, 1037
Bsp13I TCCGGA 1 cut(s) 287
Bsp143I GATC 1 cut(s) 928
Bsp19I CCATGG 1 cut(s) 924
Bsp68I TCGCGA 1 cut(s) 448
BspACI CCGC 2 cut(s) 500, 866
BspANI GGCC 2 cut(s) 669, 1025
BspCNI CTCAG 2 cut(s) 514, 901
BspEI TCCGGA 1 cut(s) 287
BspFNI CGCG 1 cut(s) 448
BspHI TCATGA 1 cut(s) 763
BspLI GGNNCC 4 cut(s) 58, 323, 668, 775
BspMI ACCTGC 1 cut(s) 805
BspPI GGATC 1 cut(s) 936
BspT104I TTCGAA 1 cut(s) 28
BspT107I GGYRCC 1 cut(s) 56
BspTNI GGTCTC 1 cut(s) 712
BsrBI CCGCTC 1 cut(s) 500
BsrDI GCAATG 1 cut(s) 697
BsrI ACTGG 4 cut(s) 154, 360, 595, 1077
BssECI CCNNGG 2 cut(s) 488, 924
BssMI GATC 1 cut(s) 928
BssNI GRCGYC 1 cut(s) 69
BssT1I CCWWGG 2 cut(s) 488, 924
Bst2UI CCWGG 2 cut(s) 272, 439
Bst4CI ACNGT 4 cut(s) 168, 199, 331, 412
Bst6I CTCTTC 2 cut(s) 8, 946
BstACI GRCGYC 1 cut(s) 69
BstBI TTCGAA 1 cut(s) 28
BstC8I GCNNGC 3 cut(s) 101, 450, 1023
BstDEI CTNAG 2 cut(s) 522, 909
BstDSI CCRYGG 1 cut(s) 924
BstENI CCTNNNNNAGG 1 cut(s) 492
BstF5I GGATG 4 cut(s) 280, 355, 616, 701
BstFNI CGCG 1 cut(s) 448
BstKTI GATC 1 cut(s) 931
BstMAI GTCTC 4 cut(s) 488, 712, 765, 1057
BstMBI GATC 1 cut(s) 928
BstMWI GCNNNNNNNGC 2 cut(s) 105, 968
BstNI CCWGG 2 cut(s) 272, 439
BstNSI RCATGY 1 cut(s) 471
BstSCI CCNGG 2 cut(s) 270, 437
BstSLI GKGCMC 1 cut(s) 1037
BstUI CGCG 1 cut(s) 448
BstV1I GCAGC 2 cut(s) 388, 975
BstV2I GAAGAC 1 cut(s) 11
Bsu36I CCTNAGG 1 cut(s) 522
BsuI GTATCC 1 cut(s) 867
BsuRI GGCC 2 cut(s) 669, 1025
BtgI CCRYGG 1 cut(s) 924
BtrI CACGTC 1 cut(s) 916
BtsCI GGATG 4 cut(s) 280, 355, 616, 701
BtsIMutI CAGTG 3 cut(s) 161, 204, 588
BtuMI TCGCGA 1 cut(s) 448
BveI ACCTGC 1 cut(s) 805
Cac8I GCNNGC 3 cut(s) 101, 450, 1023
CciI TCATGA 1 cut(s) 763
Cfr13I GGNCC 2 cut(s) 667, 773
CviAII CATG 4 cut(s) 468, 764, 845, 925
DdeI CTNAG 2 cut(s) 522, 909
DpnI GATC 1 cut(s) 930
DpnII GATC 1 cut(s) 928
Eam1104I CTCTTC 2 cut(s) 8, 946
EarI CTCTTC 2 cut(s) 8, 946
Eco130I CCWWGG 2 cut(s) 488, 924
Eco31I GGTCTC 1 cut(s) 712
Eco32I GATATC 1 cut(s) 941
Eco47I GGWCC 1 cut(s) 773
Eco81I CCTNAGG 1 cut(s) 522
EcoNI CCTNNNNNAGG 1 cut(s) 492
EcoRII CCWGG 2 cut(s) 270, 437
EcoRV GATATC 1 cut(s) 941
EcoT14I CCWWGG 2 cut(s) 488, 924
EcoT22I ATGCAT 1 cut(s) 473
ErhI CCWWGG 2 cut(s) 488, 924
FaeI CATG 4 cut(s) 471, 767, 848, 928
FalI AAGNNNNNCTT 2 cut(s) 635, 667
FaqI GGGAC 1 cut(s) 208
FatI CATG 4 cut(s) 467, 763, 844, 924
Fnu4HI GCNGC 2 cut(s) 402, 989
FokI GGATG 4 cut(s) 287, 342, 623, 708
Fsp4HI GCNGC 2 cut(s) 402, 989
FspBI CTAG 6 cut(s) 636, 687, 746, 963, 992, 1126
GluI GCNGC 2 cut(s) 402, 989
HaeIII GGCC 2 cut(s) 669, 1025
HapII CCGG 1 cut(s) 288
Hin1I GRCGYC 1 cut(s) 69
Hin1II CATG 4 cut(s) 471, 767, 848, 928
HindIII AAGCTT 1 cut(s) 555
HinfI GANTC 4 cut(s) 16, 181, 284, 304
HpaII CCGG 1 cut(s) 288
HphI GGTGA 1 cut(s) 716
Hpy166II GTNNAC 1 cut(s) 836
Hpy188I TCNGA 3 cut(s) 67, 586, 717
Hpy8I GTNNAC 1 cut(s) 836
Hpy99I CGWCG 2 cut(s) 71, 137
HpyAV CCTTC 2 cut(s) 816, 873
HpyCH4III ACNGT 4 cut(s) 168, 199, 331, 412
HpyCH4IV ACGT 4 cut(s) 69, 135, 577, 915
HpyCH4V TGCA 5 cut(s) 342, 404, 471, 851, 919
HpyF10VI GCNNNNNNNGC 2 cut(s) 105, 968
HpyF3I CTNAG 2 cut(s) 522, 909
HpySE526I ACGT 4 cut(s) 69, 135, 577, 915
Hsp92I GRCGYC 1 cut(s) 69
Hsp92II CATG 4 cut(s) 471, 767, 848, 928
Kpn2I TCCGGA 1 cut(s) 287
Kzo9I GATC 1 cut(s) 928
LmnI GCTCC 2 cut(s) 118, 497
Lsp1109I GCAGC 2 cut(s) 388, 975
LweI GCATC 2 cut(s) 265, 838
MaeI CTAG 6 cut(s) 636, 687, 746, 963, 992, 1126
MaeII ACGT 4 cut(s) 69, 135, 577, 915
MaeIII GTNAC 1 cut(s) 296
MalI GATC 1 cut(s) 930
MbiI CCGCTC 1 cut(s) 500
MboI GATC 1 cut(s) 928
MboII GAAGA 6 cut(s) 11, 14, 17, 25, 668, 963
MhlI GDGCHC 2 cut(s) 123, 1037
MluCI AATT 8 cut(s) 30, 113, 214, 230, 265, 292, 528, 1067
MlyI GAGTC 2 cut(s) 25, 190
MmeI TCCRAC 1 cut(s) 90
Mph1103I ATGCAT 1 cut(s) 473
MroI TCCGGA 1 cut(s) 287
MseI TTAA 3 cut(s) 212, 249, 981
MspA1I CMGCKG 1 cut(s) 401
MspI CCGG 1 cut(s) 288
MspR9I CCNGG 2 cut(s) 272, 439
MvaI CCWGG 2 cut(s) 272, 439
MvnI CGCG 1 cut(s) 448
MwoI GCNNNNNNNGC 2 cut(s) 105, 968
NcoI CCATGG 1 cut(s) 924
NdeII GATC 1 cut(s) 928
NlaIII CATG 4 cut(s) 471, 767, 848, 928
NlaIV GGNNCC 4 cut(s) 58, 323, 668, 775
NmeAIII GCCGAG 1 cut(s) 134
NruI TCGCGA 1 cut(s) 448
NsiI ATGCAT 1 cut(s) 473
NspI RCATGY 1 cut(s) 471
NspV TTCGAA 1 cut(s) 28
PagI TCATGA 1 cut(s) 763
PfeI GAWTC 2 cut(s) 284, 304
PfoI TCCNGGA 1 cut(s) 270
PkrI GCNGC 2 cut(s) 403, 990
PleI GAGTC 2 cut(s) 24, 189
PpsI GAGTC 2 cut(s) 24, 189
Psp6I CCWGG 2 cut(s) 270, 437
PspGI CCWGG 2 cut(s) 270, 437
PspN4I GGNNCC 4 cut(s) 58, 323, 668, 775
PspPI GGNCC 2 cut(s) 667, 773
PvuII CAGCTG 1 cut(s) 401
RruI TCGCGA 1 cut(s) 448
SaqAI TTAA 3 cut(s) 212, 249, 981
SatI GCNGC 2 cut(s) 402, 989
Sau3AI GATC 1 cut(s) 928
Sau96I GGNCC 2 cut(s) 667, 773
SchI GAGTC 2 cut(s) 25, 190
ScrFI CCNGG 2 cut(s) 272, 439
SduI GDGCHC 2 cut(s) 123, 1037
SfaNI GCATC 2 cut(s) 265, 838
SfuI TTCGAA 1 cut(s) 28
SinI GGWCC 1 cut(s) 773
Sse9I AATT 8 cut(s) 30, 113, 214, 230, 265, 292, 528, 1067
SsiI CCGC 2 cut(s) 500, 866
SspMI CTAG 6 cut(s) 636, 687, 746, 963, 992, 1126
StyD4I CCNGG 2 cut(s) 270, 437
StyI CCWWGG 2 cut(s) 488, 924
TaaI ACNGT 4 cut(s) 168, 199, 331, 412
TaiI ACGT 4 cut(s) 72, 138, 580, 918
TaqI TCGA 2 cut(s) 28, 996
TasI AATT 8 cut(s) 30, 113, 214, 230, 265, 292, 528, 1067
TfiI GAWTC 2 cut(s) 284, 304
Tru1I TTAA 3 cut(s) 212, 249, 981
Tru9I TTAA 3 cut(s) 212, 249, 981
TscAI CASTG 3 cut(s) 161, 204, 595
TseI GCWGC 2 cut(s) 401, 988
TspDTI ATGAA 1 cut(s) 1001
TspGWI ACGGA 1 cut(s) 423
TspRI CASTG 3 cut(s) 161, 204, 595
VpaK11BI GGWCC 1 cut(s) 773
XagI CCTNNNNNAGG 1 cut(s) 492
XapI RAATTY 2 cut(s) 214, 230
XceI RCATGY 1 cut(s) 471
XcmI CCANNNNNNNNNTGG 1 cut(s) 367
XspI CTAG 6 cut(s) 636, 687, 746, 963, 992, 1126
ZraI GACGTC 1 cut(s) 70
Zsp2I ATGCAT 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.