pycom03g12640

Involved in chlorophyll biosynthesis. Catalyzes the insertion of magnesium ion into protoporphyrin IX to yield Mg- protoporphyrin IX

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
N/A
Physical Location & Seq
Forward (+)
13356767 .. 13359986
3220 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 531 bp
ATGGCTTTCTCACCCACATCTTCGCCGTGTACATCTCTCTCACACCTCCATTCTTCTTCTTGTTCTTCTTCTTCGCTACTTACCCTCTTCAGATTACAGCACCCTCATCTCCTCTCTCCTTCCTTTTCTTCTTCTCTCAGTGCAAAACGTTACGAGAGCCGTCGCACTCGCATTCGCGTCACCGCTTCTGCGGCGGCTGCCATTATTGAGTCTGGAAACGGCGCCTTACTCACCCCAGAAAAGAGCTTCGACTCCATTTCCTATGGAAGACAGTTTTTTCCTTTAGCTGCGGTTGTTGGCCAGGATGCTATCAAAACTGCTTTGTTGCTTGGGGCTATAGACCGTGAAATTGGAGGAATTGCTATCTCTGGAAGGCGGGGAACAGCGAAGACAGTAATGGCACGCGGGTTGCATGCTATTTTACCACCCATTGAAGTAGTTGTTGGTTCAATGGCAAATGCTGATCCAGCCTGCCCCGAAGATTCTTGTCACAATGTCATTACAATGACCCATATATCGATTAAAAGTTAA

Protein Analysis

177

Amino Acids

18.52

Weight (kDa)

9.34

Isoelectric Point (pI)

68.06

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 221
AccII CGCG 2 cut(s) 177, 405
AciI CCGC 6 cut(s) 183, 191, 194, 290, 376, 405
AclI AACGTT 1 cut(s) 148
AclWI GGATC 1 cut(s) 458
AcoI YGGCCR 1 cut(s) 298
AcuI CTGAAG 1 cut(s) 73
AcyI GRCGYC 1 cut(s) 222
AfaI GTAC 1 cut(s) 31
AgsI TTSAA 2 cut(s) 434, 450
AjnI CCWGG 1 cut(s) 300
AjuI GAANNNNNNNTTGG 2 cut(s) 426, 458
AluBI AGCT 2 cut(s) 246, 287
AluI AGCT 2 cut(s) 246, 287
AlwI GGATC 1 cut(s) 458
AoxI GGCC 1 cut(s) 298
ApeKI GCWGC 2 cut(s) 197, 287
AspLEI GCGC 1 cut(s) 224
AsuHPI GGTGA 3 cut(s) 3, 172, 223
BalI TGGCCA 1 cut(s) 300
BanI GGYRCC 1 cut(s) 221
BbsI GAAGAC 2 cut(s) 274, 395
BbvI GCAGC 2 cut(s) 184, 274
BceAI ACGGC 3 cut(s) 10, 144, 235
BciT130I CCWGG 1 cut(s) 302
BfmI CTRYAG 1 cut(s) 336
BfoI RGCGCY 1 cut(s) 225
BisI GCNGC 4 cut(s) 192, 195, 198, 288
BlsI GCNGC 4 cut(s) 193, 196, 199, 289
Bme1390I CCNGG 1 cut(s) 302
BmiI GGNNCC 1 cut(s) 223
BmrFI CCNGG 1 cut(s) 302
BmsI GCATC 1 cut(s) 295
BpiI GAAGAC 2 cut(s) 274, 395
Bsa29I ATCGAT 1 cut(s) 518
BsaHI GRCGYC 1 cut(s) 222
BseBI CCWGG 1 cut(s) 302
BseCI ATCGAT 1 cut(s) 518
BseGI GGATG 1 cut(s) 310
BseMII CTCAG 1 cut(s) 151
BseRI GAGGAG 1 cut(s) 101
BseXI GCAGC 2 cut(s) 184, 274
Bsh1236I CGCG 2 cut(s) 177, 405
BshFI GGCC 1 cut(s) 300
BshNI GGYRCC 1 cut(s) 221
BshVI ATCGAT 1 cut(s) 518
BsmI GAATGC 1 cut(s) 171
BsnI GGCC 1 cut(s) 300
Bsp1407I TGTACA 1 cut(s) 29
Bsp143I GATC 1 cut(s) 463
BspACI CCGC 6 cut(s) 183, 191, 194, 290, 376, 405
BspANI GGCC 1 cut(s) 300
BspCNI CTCAG 1 cut(s) 150
BspDI ATCGAT 1 cut(s) 518
BspFNI CGCG 2 cut(s) 177, 405
BspLI GGNNCC 1 cut(s) 223
BspPI GGATC 1 cut(s) 458
BspT107I GGYRCC 1 cut(s) 221
BsrGI TGTACA 1 cut(s) 29
BssMI GATC 1 cut(s) 463
BssNI GRCGYC 1 cut(s) 222
Bst2UI CCWGG 1 cut(s) 302
Bst4CI ACNGT 3 cut(s) 273, 344, 394
Bst6I CTCTTC 1 cut(s) 92
BstACI GRCGYC 1 cut(s) 222
BstAUI TGTACA 1 cut(s) 29
BstC8I GCNNGC 3 cut(s) 403, 414, 472
BstDEI CTNAG 1 cut(s) 137
BstF5I GGATG 1 cut(s) 310
BstFNI CGCG 2 cut(s) 177, 405
BstH2I RGCGCY 1 cut(s) 225
BstHHI GCGC 1 cut(s) 224
BstKTI GATC 1 cut(s) 466
BstMBI GATC 1 cut(s) 463
BstMWI GCNNNNNNNGC 3 cut(s) 191, 197, 467
BstNI CCWGG 1 cut(s) 302
BstNSI RCATGY 1 cut(s) 416
BstSCI CCNGG 1 cut(s) 300
BstSFI CTRYAG 1 cut(s) 336
BstUI CGCG 2 cut(s) 177, 405
BstV1I GCAGC 2 cut(s) 184, 274
BstV2I GAAGAC 2 cut(s) 274, 395
Bsu15I ATCGAT 1 cut(s) 518
BsuRI GGCC 1 cut(s) 300
BsuTUI ATCGAT 1 cut(s) 518
BtsCI GGATG 1 cut(s) 310
BtsIMutI CAGTG 1 cut(s) 145
Cac8I GCNNGC 3 cut(s) 403, 414, 472
CfoI GCGC 1 cut(s) 224
ClaI ATCGAT 1 cut(s) 518
CseI GACGC 1 cut(s) 166
Csp6I GTAC 1 cut(s) 30
CviAII CATG 1 cut(s) 413
CviJI RGCY 8 cut(s) 5, 159, 197, 246, 287, 300, 335, 470
CviKI_1 RGCY 8 cut(s) 5, 159, 197, 246, 287, 300, 335, 470
CviQI GTAC 1 cut(s) 30
DdeI CTNAG 1 cut(s) 137
DinI GGCGCC 1 cut(s) 223
DpnI GATC 1 cut(s) 465
DpnII GATC 1 cut(s) 463
EaeI YGGCCR 1 cut(s) 298
Eam1104I CTCTTC 1 cut(s) 92
EarI CTCTTC 1 cut(s) 92
Eco57I CTGAAG 1 cut(s) 73
EcoRII CCWGG 1 cut(s) 300
EgeI GGCGCC 1 cut(s) 223
EheI GGCGCC 1 cut(s) 223
FaeI CATG 1 cut(s) 416
FaiI YATR 5 cut(s) 264, 338, 414, 513, 515
FatI CATG 1 cut(s) 412
FauI CCCGC 2 cut(s) 369, 398
Fnu4HI GCNGC 4 cut(s) 192, 195, 198, 288
FokI GGATG 1 cut(s) 317
Fsp4HI GCNGC 4 cut(s) 192, 195, 198, 288
GlaI GCGC 1 cut(s) 223
GluI GCNGC 4 cut(s) 192, 195, 198, 288
HaeII RGCGCY 1 cut(s) 225
HaeIII GGCC 1 cut(s) 300
HgaI GACGC 1 cut(s) 166
HhaI GCGC 1 cut(s) 224
Hin1I GRCGYC 1 cut(s) 222
Hin1II CATG 1 cut(s) 416
Hin6I GCGC 1 cut(s) 222
HinP1I GCGC 1 cut(s) 222
HinfI GANTC 3 cut(s) 209, 251, 482
HphI GGTGA 3 cut(s) 3, 172, 223
Hpy166II GTNNAC 1 cut(s) 30
Hpy188I TCNGA 1 cut(s) 92
Hpy188III TCNNGA 2 cut(s) 213, 369
Hpy8I GTNNAC 1 cut(s) 30
Hpy99I CGWCG 1 cut(s) 165
HpyAV CCTTC 2 cut(s) 129, 366
HpyCH4III ACNGT 3 cut(s) 273, 344, 394
HpyCH4IV ACGT 1 cut(s) 148
HpyCH4V TGCA 2 cut(s) 143, 412
HpyF10VI GCNNNNNNNGC 3 cut(s) 191, 197, 467
HpyF3I CTNAG 1 cut(s) 137
HpySE526I ACGT 1 cut(s) 148
Hsp92I GRCGYC 1 cut(s) 222
Hsp92II CATG 1 cut(s) 416
HspAI GCGC 1 cut(s) 222
KasI GGCGCC 1 cut(s) 221
Kzo9I GATC 1 cut(s) 463
LpnPI CCDG 7 cut(s) 198, 249, 287, 314, 354, 480, 484
Lsp1109I GCAGC 2 cut(s) 184, 274
LweI GCATC 1 cut(s) 295
MaeII ACGT 1 cut(s) 148
MaeIII GTNAC 3 cut(s) 149, 178, 488
MalI GATC 1 cut(s) 465
MboI GATC 1 cut(s) 463
MlsI TGGCCA 1 cut(s) 300
MluCI AATT 2 cut(s) 348, 357
MluNI TGGCCA 1 cut(s) 300
Mly113I GGCGCC 1 cut(s) 222
MlyI GAGTC 2 cut(s) 218, 245
MnlI CCTC 5 cut(s) 56, 95, 114, 122, 347
Mox20I TGGCCA 1 cut(s) 300
MscI TGGCCA 1 cut(s) 300
MseI TTAA 2 cut(s) 522, 529
MslI CAYNNNNRTG 1 cut(s) 503
Msp20I TGGCCA 1 cut(s) 300
MspR9I CCNGG 1 cut(s) 302
Mva1269I GAATGC 1 cut(s) 171
MvaI CCWGG 1 cut(s) 302
MvnI CGCG 2 cut(s) 177, 405
MwoI GCNNNNNNNGC 3 cut(s) 191, 197, 467
NarI GGCGCC 1 cut(s) 222
NdeII GATC 1 cut(s) 463
NlaIII CATG 1 cut(s) 416
NlaIV GGNNCC 1 cut(s) 223
NmuCI GTSAC 2 cut(s) 178, 488
NspI RCATGY 1 cut(s) 416
PaeI GCATGC 1 cut(s) 416
PctI GAATGC 1 cut(s) 171
PfeI GAWTC 1 cut(s) 482
PkrI GCNGC 4 cut(s) 193, 196, 199, 289
PleI GAGTC 2 cut(s) 217, 245
PluTI GGCGCC 1 cut(s) 225
PpsI GAGTC 2 cut(s) 217, 245
Psp1406I AACGTT 1 cut(s) 148
Psp6I CCWGG 1 cut(s) 300
PspGI CCWGG 1 cut(s) 300
PspN4I GGNNCC 1 cut(s) 223
RsaI GTAC 1 cut(s) 31
RsaNI GTAC 1 cut(s) 30
RseI CAYNNNNRTG 1 cut(s) 503
SaqAI TTAA 2 cut(s) 522, 529
SatI GCNGC 4 cut(s) 192, 195, 198, 288
Sau3AI GATC 1 cut(s) 463
SchI GAGTC 2 cut(s) 218, 245
ScrFI CCNGG 1 cut(s) 302
SetI ASST 4 cut(s) 48, 151, 248, 289
SfaNI GCATC 1 cut(s) 295
SfcI CTRYAG 1 cut(s) 336
SfoI GGCGCC 1 cut(s) 223
SmiMI CAYNNNNRTG 1 cut(s) 503
SphI GCATGC 1 cut(s) 416
Sse9I AATT 2 cut(s) 348, 357
SsiI CCGC 6 cut(s) 183, 191, 194, 290, 376, 405
SspDI GGCGCC 1 cut(s) 221
StyD4I CCNGG 1 cut(s) 300
TaaI ACNGT 3 cut(s) 273, 344, 394
TaiI ACGT 1 cut(s) 151
TaqI TCGA 2 cut(s) 249, 518
TasI AATT 2 cut(s) 348, 357
TatI WGTACW 1 cut(s) 29
TauI GCSGC 2 cut(s) 194, 197
TfiI GAWTC 1 cut(s) 482
Tru1I TTAA 2 cut(s) 522, 529
Tru9I TTAA 2 cut(s) 522, 529
TscAI CASTG 1 cut(s) 145
TseFI GTSAC 2 cut(s) 178, 488
TseI GCWGC 2 cut(s) 197, 287
Tsp45I GTSAC 2 cut(s) 178, 488
TspRI CASTG 1 cut(s) 145
XceI RCATGY 1 cut(s) 416
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.