pycom03g13460

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
N/A
Physical Location & Seq
Forward (+)
14485479 .. 14489294
3816 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2811 bp
ATGGATAAAAGTCGTTTCCTACTGTTGAGAATGTTGTCAATGATACACTGCTTGTGTTATATCTATATGATGATTATGGCTAATTACTTAACTGCAGGTGCATTAGCAATAGCTCATACCAATTTCAGCACAGATCAGTCTGCGCTTCTTGCTCTCAAAGCTCACATCACTAGTGACCCTCAAAACATCTTGACCGCCAACTGGTCGTCTGCCTCCAATTCTGACATTTGCAACTGGGTTGGTGTTACCTGCGGTGCTCGCCACCATAGAGTCACGGCCTTAAATCTCTCGTCCATGGGTCTTGCTGGGGTTATTCCTCCGCATCTAGGCAACCTCTCATTTCTCATTGAGTTGCACCTTAAGAATAATTTTTTTAATGGTCCCCTACCCCAAGAATTGTCTCGTTTGCGCCGGTTGAAGGCGATTAACTTTCAATACAACAACTTTACGGGAACCATTCCTTCGTGGTTTGGGTCCTTCCCTAAACTTCAAACCTTCATATTGTGGGGTAATGGCTTCTCTGGTTTCATACCCGCTGCTATCTTCAACTTATCTGCACTTGAAAAAATTGATCTGAGCAATAACCAACTATCAGGTAGCATACCCAGAGAAATCGGCAACTTAACAATGGTGAAGCGCATATACCTTGACGACAACAAGTTCGAAGAACTCCCGAACGAGATAGGCAGTTTAGGTCAGCTGGAGGTGTTGTTTGTGCAGGACAATGCTCTAAAAGGCTCTGCTTTTGTGCCTGTCCTCAACATATCTTCTTTGACTACTTTCACTCTATACGGAAACAACATGAGTGGTAGTCTTCCGGACAATATGTGTGAGCATCTTTCGAGTATTCGACGATTATATTTGGATCAAAACCAGCTTGACGGTATGATTCCCTCCAAACTGTGGCAATGCAAAGAGCTTCGTGAAATCTCATTAGCGTCTAACAATTTCCGTGGAAGCATACCCAAAAGTCTTGGCAATTTGACCTACTTAAGCAAGATTATTCTTTCCGACAATAATTTAGAAGGTACAATACCGGATGAGATTGGTGATCTTCCACAGTTAGAGATATTGACACTGCATGCTAATAATCTCAGTGGCGTCATCCCATCCAAACTCTTCAATAACTCCATGGTAAGAGAAATAGGGCTTTCTATTAATCAGCTCTCAGGTGGCCTCCCAGCAAACATAGGTCTTAACATTCCAAACCTAGAATTCCTAGGTGTGGCCCAAAATAACCTCGTGGCCGGACCACTCCCTAACCTCTCCAATGCTTCCAAGCTCAAGGAGCTAGACATGAGCATAAACTCATTTACCGGGTTTCTTCCTATCACACTCTGTTCCTTAAAAAACCTCGAGTTTCTTATCTTGCATTCGAACAATTTGACGATTGATGCTTCTACTCCACAAGTAGGAAGCACTCTTTCTTGCTTGTTTAATCTTAGAAATCTAACAAGATTATACTTGTCAAATAATCCACTAAACACCAAAATTCCAGCTCCTCGCGGGAATCTATCTACGTCACTCCAATATGTTGATTTAAGATTTTCCAACATCAAGGGTAACATTCCAGTTGATATTGGCAACTTGAGCAGCATGATAGAGTTAAACCTGGGAGACAATCAGTTGAGTGGAGCAATTCCAAGTTCAATACAAAGGCTAAAAAATCTCCAAGATGGACCACTCTCAGAGGATATCGGAAAGTTGAGAGATGTGGTGGATATGGATTTATCAAATAACCATTTCTCCGGAAACATTCCTGGTAGCATTGGTGGTCTTCAAAATATGATTAGTTTCTCCTTGGCAAACAATTCTTTAGAAGGCCCTATTCCCAGTTCATTTAAAACCTTGCTAAGCCTGGAATTCTTGGATTTGTCCAAAAACAATCTATCCGGGGTGATCCCAAAGTCATTGGAAGCACTCTTGTATCTCAAGCATCTGAATTTGTCTTTCAACAAACTCCAAGGAGAAATTCCGACCGGCGGGCCTTTCGGAAACTTCTCTGCTGATTCATTTGCATCAAACGGTGCACTCTGCGGTGCTTCTCGACTCCATGTTCCACTATGCAAAAATAGAACAAAAGTTAAGCCGAACTGGAGGAAAGCTAAATATATCATCCCAGGGGTCATATCAGTAATACTCCTAGCGGCCGCTGCATTGATTCTGATACAATGCAAGAAAAGGAATGTCGAAGTTGTTAGAGAAACTGCCTCGCTACATCAAGTTCTTTGGAGAAGAGTTTCGCGCCTAGAACTTGTAAGGGCAACAAATGGATTTCATGAGAGTAACTTACTAGGCACAGGTGGTTTTGGCTCAGTGTACAGGGGAACACTTTCAGACAGGATAGATATCGCCGTCAAGGTTTTCAATTTACAGCTAGAAGAGGCATTCAAAAGTTTTGAGAAGGAATGTGAAATGCTAAGCAATATCCGTCACCGGAATCTTATTAAAATCATAAGTTGCTGCAATGATCTTGATTTCAAAGCCCTGATACTTCAATTGATGCCTAATGGAAGCCTCGAAAAGTGGTTGTATTCTCCAAACCGCTCCATGACTCTCCTGCAAAGGTTAGATATAATGAAAGATGTTGCATTGGCACTAGAATATCTTCATCATGGTTACTCGATACCTATCGTTCATTGTGATGTGAAACCAAGCAATATATTACTAGATGATGATATGGTTGCACATGTTGCTGATTTTGGCATTGCAAGACTCATTGGCGGTGGAGATTCGATGACAGAAACCATGACCCTAGCTACAATTGGATATATGGCTCCAGTCATTATTTCCAGATGCTGCAATAGGTGA

Protein Analysis

937

Amino Acids

102.92

Weight (kDa)

8.32

Isoelectric Point (pI)

33.1

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRRNT_2 PF08263 44 - 84 5.8e-10 Leucine rich repeat N-terminal domain
LRR_8 PF13855 138 - 197 9.7e-07 Leucine rich repeat
LRR_8 PF13855 161 - 221 2.9e-08 Leucine rich repeat
LRR_14 PF23598 182 - 266 5.2e-06 Leucine-rich repeat region
LRR_8 PF13855 257 - 317 1e-05 Leucine rich repeat
LRR_8 PF13855 306 - 365 5.3e-07 Leucine rich repeat
LRR_8 PF13855 600 - 654 7.9e-06 Leucine rich repeat
Pkinase PF00069 759 - 931 7.9e-32 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 762 - 931 3.9e-30 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 86
Acc36I ACCTGC 2 cut(s) 86, 257
AccB7I CCANNNNNTGG 1 cut(s) 903
AccBSI CCGCTC 1 cut(s) 2547
AccII CGCG 2 cut(s) 1506, 2245
AccIII TCCGGA 2 cut(s) 817, 1748
AclWI GGATC 2 cut(s) 873, 1894
AcoI YGGCCR 2 cut(s) 1245, 2148
AcsI RAATTY 5 cut(s) 1214, 1491, 1862, 1942, 1971
AcyI GRCGYC 1 cut(s) 1101
AfaI GTAC 2 cut(s) 1030, 2321
AfiI CCNNNNNNNGG 7 cut(s) 201, 326, 418, 903, 1225, 1412, 1982
AflII CTTAAG 2 cut(s) 359, 991
AflIII ACRYGT 1 cut(s) 2689
AhlI ACTAGT 1 cut(s) 170
AjnI CCWGG 4 cut(s) 1611, 1759, 1857, 2119
AjuI GAANNNNNNNTTGG 2 cut(s) 1956, 1988
Alw21I GWGCWC 2 cut(s) 259, 2032
Alw26I GTCTC 2 cut(s) 405, 1611
Alw44I GTGCAC 1 cut(s) 2028
AlwI GGATC 2 cut(s) 873, 1894
AlwNI CAGNNNCTG 1 cut(s) 2799
Ama87I CYCGRG 1 cut(s) 1355
Aor13HI TCCGGA 2 cut(s) 817, 1748
AoxI GGCC 7 cut(s) 276, 1174, 1227, 1245, 1822, 1985, 2148
ApaLI GTGCAC 1 cut(s) 2028
ApeKI GCWGC 5 cut(s) 536, 1593, 2153, 2463, 2799
ApoI RAATTY 5 cut(s) 1214, 1491, 1862, 1942, 1971
ArsI GACNNNNNNTTYG 2 cut(s) 644, 676
AseI ATTAAT 1 cut(s) 1158
Asp700I GAANNNNTTC 3 cut(s) 2239, 2332, 2607
AspA2I CCTAGG 1 cut(s) 1219
AspLEI GCGC 4 cut(s) 145, 411, 639, 2247
AspS9I GGNCC 7 cut(s) 380, 474, 1228, 1250, 1679, 1823, 1985
AsuC2I CCSGG 2 cut(s) 1318, 1894
AsuHPI GGTGA 4 cut(s) 643, 1061, 1909, 2426
AsuII TTCGAA 2 cut(s) 663, 1376
AvaI CYCGRG 1 cut(s) 1355
AvaII GGWCC 4 cut(s) 380, 474, 1250, 1679
AvrII CCTAGG 1 cut(s) 1219
BaeGI GKGCMC 1 cut(s) 2032
BaeI ACNNNNGTAYC 2 cut(s) 1910, 1943
BarI GAAGNNNNNNTAC 4 cut(s) 626, 658, 1017, 1049
BauI CACGAG 1 cut(s) 1241
BbsI GAAGAC 2 cut(s) 806, 1769
Bbv12I GWGCWC 2 cut(s) 259, 2032
BbvI GCAGC 5 cut(s) 523, 1605, 2140, 2450, 2786
BccI CCATC 2 cut(s) 1117, 1670
BceAI ACGGC 2 cut(s) 291, 2339
BciT130I CCWGG 4 cut(s) 1613, 1761, 1859, 2121
BcnI CCSGG 2 cut(s) 1318, 1894
BcoDI GTCTC 2 cut(s) 405, 1611
BcuI ACTAGT 1 cut(s) 170
BfmI CTRYAG 1 cut(s) 93
BfrI CTTAAG 2 cut(s) 359, 991
BfuAI ACCTGC 2 cut(s) 86, 257
BisI GCNGC 7 cut(s) 537, 1594, 2148, 2151, 2154, 2464, 2800
BlnI CCTAGG 1 cut(s) 1219
BlpI GCTNAGC 2 cut(s) 1853, 2420
BlsI GCNGC 7 cut(s) 538, 1595, 2149, 2152, 2155, 2465, 2801
Bme1390I CCNGG 6 cut(s) 1318, 1613, 1761, 1859, 1894, 2121
Bme18I GGWCC 4 cut(s) 380, 474, 1250, 1679
BmeT110I CYCGRG 1 cut(s) 1355
BmgT120I GGNCC 7 cut(s) 380, 474, 1228, 1250, 1679, 1823, 1985
BmiI GGNNCC 4 cut(s) 382, 454, 475, 2778
BmrFI CCNGG 6 cut(s) 1318, 1613, 1761, 1859, 1894, 2121
BmrI ACTGGG 2 cut(s) 244, 1827
BmsI GCATC 7 cut(s) 331, 844, 1384, 1945, 2027, 2493, 2786
BmuI ACTGGG 2 cut(s) 244, 1827
BpiI GAAGAC 2 cut(s) 806, 1769
BpmI CTGGAG 3 cut(s) 722, 2116, 2763
Bpu1102I GCTNAGC 2 cut(s) 1853, 2420
Bpu14I TTCGAA 2 cut(s) 663, 1376
BpuEI CTTGAG 3 cut(s) 1268, 1609, 1916
BpuMI CCSGG 2 cut(s) 1318, 1894
BsaBI GATNNNNATC 2 cut(s) 2348, 2630
BsaHI GRCGYC 1 cut(s) 1101
BsaWI WCCGGW 4 cut(s) 817, 1036, 1748, 2436
BsaXI ACNNNNNCTCC 4 cut(s) 695, 725, 1730, 1760
Bsc4I CCNNNNNNNGG 7 cut(s) 201, 326, 418, 903, 1225, 1412, 1982
Bse118I RCCGGY 2 cut(s) 411, 1979
Bse1I ACTGG 6 cut(s) 206, 239, 1571, 1833, 2099, 2780
Bse3DI GCAATG 3 cut(s) 914, 2473, 2706
Bse8I GATNNNNATC 2 cut(s) 2348, 2630
BseAI TCCGGA 2 cut(s) 817, 1748
BseBI CCWGG 4 cut(s) 1613, 1761, 1859, 2121
BseGI GGATG 4 cut(s) 1045, 1104, 1109, 2115
BseJI GATNNNNATC 2 cut(s) 2348, 2630
BseLI CCNNNNNNNGG 7 cut(s) 201, 326, 418, 903, 1225, 1412, 1982
BseMI GCAATG 3 cut(s) 914, 2473, 2706
BseMII CTCAG 5 cut(s) 566, 1108, 1182, 1701, 2328
BseNI ACTGG 6 cut(s) 206, 239, 1571, 1833, 2099, 2780
BseRI GAGGAG 1 cut(s) 1491
BseSI GKGCMC 1 cut(s) 2032
BseX3I CGGCCG 1 cut(s) 2148
BseXI GCAGC 5 cut(s) 523, 1605, 2140, 2450, 2786
BseYI CCCAGC 2 cut(s) 305, 1180
BsgI GTGCAG 2 cut(s) 540, 737
Bsh1236I CGCG 2 cut(s) 1506, 2245
Bsh1285I CGRYCG 2 cut(s) 1980, 2151
BshFI GGCC 7 cut(s) 278, 1176, 1229, 1247, 1824, 1987, 2150
BsiEI CGRYCG 2 cut(s) 1980, 2151
BsiHKAI GWGCWC 2 cut(s) 259, 2032
BsiHKCI CYCGRG 1 cut(s) 1355
BsiSI CCGG 9 cut(s) 412, 818, 1037, 1248, 1317, 1749, 1893, 1980, 2437
BslFI GGGAC 1 cut(s) 366
BslI CCNNNNNNNGG 7 cut(s) 201, 326, 418, 903, 1225, 1412, 1982
BsmAI GTCTC 2 cut(s) 405, 1611
BsmFI GGGAC 1 cut(s) 366
BsmI GAATGC 2 cut(s) 1372, 2387
BsnI GGCC 7 cut(s) 278, 1176, 1229, 1247, 1824, 1987, 2150
BsoBI CYCGRG 1 cut(s) 1355
Bsp119I TTCGAA 2 cut(s) 663, 1376
Bsp1286I GDGCHC 2 cut(s) 259, 2032
Bsp13I TCCGGA 2 cut(s) 817, 1748
Bsp1407I TGTACA 1 cut(s) 2319
Bsp143I GATC 6 cut(s) 133, 571, 865, 1051, 1899, 2470
Bsp1720I GCTNAGC 2 cut(s) 1853, 2420
Bsp19I CCATGG 2 cut(s) 294, 1131
BspANI GGCC 7 cut(s) 278, 1176, 1229, 1247, 1824, 1987, 2150
BspCNI CTCAG 5 cut(s) 567, 1107, 1181, 1700, 2327
BspEI TCCGGA 2 cut(s) 817, 1748
BspFNI CGCG 2 cut(s) 1506, 2245
BspHI TCATGA 1 cut(s) 2278
BspLI GGNNCC 4 cut(s) 382, 454, 475, 2778
BspMAI CTGCAG 1 cut(s) 97
BspMI ACCTGC 2 cut(s) 86, 257
BspPI GGATC 2 cut(s) 873, 1894
BspT104I TTCGAA 2 cut(s) 663, 1376
BspTI CTTAAG 2 cut(s) 359, 991
BsrBI CCGCTC 1 cut(s) 2547
BsrDI GCAATG 3 cut(s) 914, 2473, 2706
BsrFI RCCGGY 2 cut(s) 411, 1979
BsrGI TGTACA 1 cut(s) 2319
BsrI ACTGG 6 cut(s) 206, 239, 1571, 1833, 2099, 2780
BssAI RCCGGY 2 cut(s) 411, 1979
BssMI GATC 6 cut(s) 133, 571, 865, 1051, 1899, 2470
BssNI GRCGYC 1 cut(s) 1101
BssSI CACGAG 1 cut(s) 1241
BssT1I CCWWGG 5 cut(s) 294, 1131, 1219, 1800, 1963
Bst2BI CACGAG 1 cut(s) 1241
Bst2UI CCWGG 4 cut(s) 1613, 1761, 1859, 2121
Bst4CI ACNGT 5 cut(s) 24, 884, 903, 1062, 2027
Bst6I CTCTTC 3 cut(s) 1124, 2230, 2376
BstACI GRCGYC 1 cut(s) 1101
BstAFI CTTAAG 2 cut(s) 359, 991
BstAPI GCANNNNNTGC 1 cut(s) 2693
BstAUI TGTACA 1 cut(s) 2319
BstBI TTCGAA 2 cut(s) 663, 1376
BstC8I GCNNGC 3 cut(s) 259, 1083, 1985
BstDEI CTNAG 8 cut(s) 575, 1094, 1168, 1442, 1687, 1853, 2314, 2420
BstDSI CCRYGG 3 cut(s) 294, 952, 1131
BstF5I GGATG 4 cut(s) 1045, 1104, 1109, 2115
BstFNI CGCG 2 cut(s) 1506, 2245
BstHHI GCGC 4 cut(s) 145, 411, 639, 2247
BstKTI GATC 6 cut(s) 136, 574, 868, 1054, 1902, 2473
BstMAI GTCTC 2 cut(s) 405, 1611
BstMBI GATC 6 cut(s) 133, 571, 865, 1051, 1899, 2470
BstMCI CGRYCG 2 cut(s) 1980, 2151
BstMWI GCNNNNNNNGC 7 cut(s) 149, 158, 258, 1288, 1590, 2153, 2693
BstNI CCWGG 4 cut(s) 1613, 1761, 1859, 2121
BstNSI RCATGY 2 cut(s) 1085, 2693
BstSCI CCNGG 6 cut(s) 1316, 1611, 1759, 1857, 1892, 2119
BstSFI CTRYAG 1 cut(s) 93
BstSLI GKGCMC 1 cut(s) 2032
BstUI CGCG 2 cut(s) 1506, 2245
BstV1I GCAGC 5 cut(s) 523, 1605, 2140, 2450, 2786
BstV2I GAAGAC 2 cut(s) 806, 1769
BstZI CGGCCG 1 cut(s) 2148
BsuRI GGCC 7 cut(s) 278, 1176, 1229, 1247, 1824, 1987, 2150
BtgI CCRYGG 3 cut(s) 294, 952, 1131
BtsCI GGATG 4 cut(s) 1045, 1104, 1109, 2115
BtsI GCAGTG 2 cut(s) 46, 1076
BtsIMutI CAGTG 4 cut(s) 46, 1076, 1102, 2322
BveI ACCTGC 2 cut(s) 86, 257
Cac8I GCNNGC 3 cut(s) 259, 1083, 1985
CaiI CAGNNNCTG 1 cut(s) 2799
CciI TCATGA 1 cut(s) 2278
CciNI GCGGCCGC 1 cut(s) 2148
CfoI GCGC 4 cut(s) 145, 411, 639, 2247
Cfr10I RCCGGY 2 cut(s) 411, 1979
Cfr13I GGNCC 7 cut(s) 380, 474, 1228, 1250, 1679, 1823, 1985
CseI GACGC 2 cut(s) 927, 1090
Csp6I GTAC 2 cut(s) 1029, 2320
CspCI CAANNNNNGTGG 4 cut(s) 787, 822, 934, 969
CviQI GTAC 2 cut(s) 1029, 2320
DdeI CTNAG 8 cut(s) 575, 1094, 1168, 1442, 1687, 1853, 2314, 2420
DpnI GATC 6 cut(s) 135, 573, 867, 1053, 1901, 2472
DpnII GATC 6 cut(s) 133, 571, 865, 1051, 1899, 2470
DraI TTTAAA 1 cut(s) 1843
EaeI YGGCCR 2 cut(s) 1245, 2148
EagI CGGCCG 1 cut(s) 2148
Eam1104I CTCTTC 3 cut(s) 1124, 2230, 2376
EarI CTCTTC 3 cut(s) 1124, 2230, 2376
EclXI CGGCCG 1 cut(s) 2148
Eco130I CCWWGG 5 cut(s) 294, 1131, 1219, 1800, 1963
Eco32I GATATC 2 cut(s) 1696, 2350
Eco47I GGWCC 4 cut(s) 380, 474, 1250, 1679
Eco52I CGGCCG 1 cut(s) 2148
Eco88I CYCGRG 1 cut(s) 1355
EcoO109I RGGNCCY 2 cut(s) 474, 1823
EcoRI GAATTC 2 cut(s) 1214, 1862
EcoRII CCWGG 4 cut(s) 1611, 1759, 1857, 2119
EcoRV GATATC 2 cut(s) 1696, 2350
EcoT14I CCWWGG 5 cut(s) 294, 1131, 1219, 1800, 1963
ErhI CCWWGG 5 cut(s) 294, 1131, 1219, 1800, 1963
FaqI GGGAC 1 cut(s) 366
FauI CCCGC 3 cut(s) 541, 1499, 1976
Fnu4HI GCNGC 7 cut(s) 537, 1594, 2148, 2151, 2154, 2464, 2800
FokI GGATG 4 cut(s) 1052, 1091, 1096, 2102
Fsp4HI GCNGC 7 cut(s) 537, 1594, 2148, 2151, 2154, 2464, 2800
GlaI GCGC 4 cut(s) 144, 410, 638, 2246
GluI GCNGC 7 cut(s) 537, 1594, 2148, 2151, 2154, 2464, 2800
GsaI CCCAGC 2 cut(s) 309, 1184
GsuI CTGGAG 3 cut(s) 722, 2116, 2763
HaeIII GGCC 7 cut(s) 278, 1176, 1229, 1247, 1824, 1987, 2150
HapII CCGG 9 cut(s) 412, 818, 1037, 1248, 1317, 1749, 1893, 1980, 2437
HgaI GACGC 2 cut(s) 927, 1090
HhaI GCGC 4 cut(s) 145, 411, 639, 2247
Hin1I GRCGYC 1 cut(s) 1101
Hin6I GCGC 4 cut(s) 143, 409, 637, 2245
HinP1I GCGC 4 cut(s) 143, 409, 637, 2245
HpaII CCGG 9 cut(s) 412, 818, 1037, 1248, 1317, 1749, 1893, 1980, 2437
HphI GGTGA 4 cut(s) 643, 1061, 1909, 2426
Hpy166II GTNNAC 2 cut(s) 2030, 2320
Hpy188III TCNNGA 9 cut(s) 190, 673, 818, 923, 1749, 2046, 2279, 2474, 2793
Hpy8I GTNNAC 2 cut(s) 2030, 2320
Hpy99I CGWCG 1 cut(s) 855
HpyAV CCTTC 7 cut(s) 412, 471, 487, 505, 1019, 1814, 2398
HpyCH4III ACNGT 5 cut(s) 24, 884, 903, 1062, 2027
HpyCH4IV ACGT 1 cut(s) 1520
HpyF10VI GCNNNNNNNGC 7 cut(s) 149, 158, 258, 1288, 1590, 2153, 2693
HpyF3I CTNAG 8 cut(s) 575, 1094, 1168, 1442, 1687, 1853, 2314, 2420
HpySE526I ACGT 1 cut(s) 1520
Hsp92I GRCGYC 1 cut(s) 1101
HspAI GCGC 4 cut(s) 143, 409, 637, 2245
Kpn2I TCCGGA 2 cut(s) 817, 1748
Kzo9I GATC 6 cut(s) 133, 571, 865, 1051, 1899, 2470
LmnI GCTCC 5 cut(s) 1288, 1504, 1634, 2552, 2782
Lsp1109I GCAGC 5 cut(s) 523, 1605, 2140, 2450, 2786
LweI GCATC 7 cut(s) 331, 844, 1384, 1945, 2027, 2493, 2786
MaeII ACGT 1 cut(s) 1520
MaeIII GTNAC 8 cut(s) 173, 244, 271, 1521, 1562, 2285, 2432, 2618
MalI GATC 6 cut(s) 135, 573, 867, 1053, 1901, 2472
MbiI CCGCTC 1 cut(s) 2547
MboI GATC 6 cut(s) 133, 571, 865, 1051, 1899, 2470
MfeI CAATTG 2 cut(s) 2498, 2763
MhlI GDGCHC 2 cut(s) 259, 2032
MlyI GAGTC 4 cut(s) 279, 2043, 2548, 2709
MmeI TCCRAC 3 cut(s) 1035, 1575, 2000
MroI TCCGGA 2 cut(s) 817, 1748
MroXI GAANNNNTTC 3 cut(s) 2239, 2332, 2607
MslI CAYNNNNRTG 1 cut(s) 2643
MspA1I CMGCKG 3 cut(s) 536, 700, 2153
MspCI CTTAAG 2 cut(s) 359, 991
MspI CCGG 9 cut(s) 412, 818, 1037, 1248, 1317, 1749, 1893, 1980, 2437
MspR9I CCNGG 6 cut(s) 1318, 1613, 1761, 1859, 1894, 2121
MunI CAATTG 2 cut(s) 2498, 2763
Mva1269I GAATGC 2 cut(s) 1372, 2387
MvaI CCWGG 4 cut(s) 1613, 1761, 1859, 2121
MvnI CGCG 2 cut(s) 1506, 2245
MwoI GCNNNNNNNGC 7 cut(s) 149, 158, 258, 1288, 1590, 2153, 2693
NciI CCSGG 2 cut(s) 1318, 1894
NcoI CCATGG 2 cut(s) 294, 1131
NdeII GATC 6 cut(s) 133, 571, 865, 1051, 1899, 2470
NlaIV GGNNCC 4 cut(s) 382, 454, 475, 2778
NmuCI GTSAC 4 cut(s) 173, 271, 1521, 2432
NotI GCGGCCGC 1 cut(s) 2148
NspI RCATGY 2 cut(s) 1085, 2693
NspV TTCGAA 2 cut(s) 663, 1376
PaeI GCATGC 1 cut(s) 1085
PaeR7I CTCGAG 1 cut(s) 1355
PagI TCATGA 1 cut(s) 2278
PaqCI CACCTGC 1 cut(s) 86
PasI CCCWGGG 1 cut(s) 2120
PciI ACATGT 1 cut(s) 2689
PctI GAATGC 2 cut(s) 1372, 2387
PdmI GAANNNNTTC 3 cut(s) 2239, 2332, 2607
PfeI GAWTC 6 cut(s) 889, 1510, 2009, 2161, 2440, 2732
PflMI CCANNNNNTGG 1 cut(s) 903
PkrI GCNGC 7 cut(s) 538, 1595, 2149, 2152, 2155, 2465, 2801
PleI GAGTC 4 cut(s) 278, 2043, 2548, 2709
PpsI GAGTC 4 cut(s) 278, 2043, 2548, 2709
PpuMI RGGWCCY 1 cut(s) 474
PscI ACATGT 1 cut(s) 2689
PshBI ATTAAT 1 cut(s) 1158
Psp5II RGGWCCY 1 cut(s) 474
Psp6I CCWGG 4 cut(s) 1611, 1759, 1857, 2119
PspFI CCCAGC 2 cut(s) 305, 1180
PspGI CCWGG 4 cut(s) 1611, 1759, 1857, 2119
PspN4I GGNNCC 4 cut(s) 382, 454, 475, 2778
PspPI GGNCC 7 cut(s) 380, 474, 1228, 1250, 1679, 1823, 1985
PspPPI RGGWCCY 1 cut(s) 474
PspXI VCTCGAGB 1 cut(s) 1355
PstI CTGCAG 1 cut(s) 97
PstNI CAGNNNCTG 1 cut(s) 2799
PvuII CAGCTG 1 cut(s) 700
RsaI GTAC 2 cut(s) 1030, 2321
RsaNI GTAC 2 cut(s) 1029, 2320
RseI CAYNNNNRTG 1 cut(s) 2643
SatI GCNGC 7 cut(s) 537, 1594, 2148, 2151, 2154, 2464, 2800
Sau3AI GATC 6 cut(s) 133, 571, 865, 1051, 1899, 2470
Sau96I GGNCC 7 cut(s) 380, 474, 1228, 1250, 1679, 1823, 1985
SchI GAGTC 4 cut(s) 279, 2043, 2548, 2709
ScrFI CCNGG 6 cut(s) 1318, 1613, 1761, 1859, 1894, 2121
SduI GDGCHC 2 cut(s) 259, 2032
SfaNI GCATC 7 cut(s) 331, 844, 1384, 1945, 2027, 2493, 2786
SfcI CTRYAG 1 cut(s) 93
Sfr274I CTCGAG 1 cut(s) 1355
SfuI TTCGAA 2 cut(s) 663, 1376
SinI GGWCC 4 cut(s) 380, 474, 1250, 1679
SlaI CTCGAG 1 cut(s) 1355
SmiMI CAYNNNNRTG 1 cut(s) 2643
SmlI CTYRAG 6 cut(s) 359, 991, 1283, 1355, 1588, 1931
SmoI CTYRAG 6 cut(s) 359, 991, 1283, 1355, 1588, 1931
SpeI ACTAGT 1 cut(s) 170
SphI GCATGC 1 cut(s) 1085
StyD4I CCNGG 6 cut(s) 1316, 1611, 1759, 1857, 1892, 2119
StyI CCWWGG 5 cut(s) 294, 1131, 1219, 1800, 1963
TaaI ACNGT 5 cut(s) 24, 884, 903, 1062, 2027
TaiI ACGT 1 cut(s) 1523
TatI WGTACW 1 cut(s) 2319
TauI GCSGC 2 cut(s) 2150, 2153
TfiI GAWTC 6 cut(s) 889, 1510, 2009, 2161, 2440, 2732
TscAI CASTG 4 cut(s) 53, 1083, 1102, 2322
TseFI GTSAC 4 cut(s) 173, 271, 1521, 2432
TseI GCWGC 5 cut(s) 536, 1593, 2153, 2463, 2799
Tsp45I GTSAC 4 cut(s) 173, 271, 1521, 2432
TspDTI ATGAA 8 cut(s) 487, 517, 1827, 2001, 2267, 2594, 2600, 2627
TspGWI ACGGA 3 cut(s) 807, 941, 2420
TspRI CASTG 4 cut(s) 53, 1083, 1102, 2322
Van91I CCANNNNNTGG 1 cut(s) 903
Vha464I CTTAAG 2 cut(s) 359, 991
VneI GTGCAC 1 cut(s) 2028
VpaK11BI GGWCC 4 cut(s) 380, 474, 1250, 1679
VspI ATTAAT 1 cut(s) 1158
XapI RAATTY 5 cut(s) 1214, 1491, 1862, 1942, 1971
XceI RCATGY 2 cut(s) 1085, 2693
XhoI CTCGAG 1 cut(s) 1355
XmaJI CCTAGG 1 cut(s) 1219
XmnI GAANNNNTTC 3 cut(s) 2239, 2332, 2607
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.