pycom03g13560

LRR receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
N/A
Physical Location & Seq
Forward (+)
14670802 .. 14672181
1380 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 456 bp
ATGGCCTTAAATCTCTCGTACATGGGTCTTGCCGGGGTTATTCCTCCGCATCTAGGCAACCTCTCATTTCTCGTTGAGTTGGGCCTTAGGAATAATAGTTTTCATGGTCCCCTACCCCAAGAACTGTCTCGTTTGCGCCGGTTGAAGGTGATTAACTTTCGATACAACAACTTTATGGGAACCATTCCTTCGTGGTTTGGGTCCTTCCCTAAACTTCAAACCTTCATATTGTGGGGTAATGGCTTCTCTGGTTTCATACCCGCTGCTATCTTCAACTTATCTGCACTCGAACACATTGATCTGAGCAGTAATCAACTATCAGGTAGCATACCCAGAGAAATCGGCAACTTAACAATGTTGAAGCGCATATACCTTGACGACAACAAGTTCGAAGGTACAATACCGGATGAGATTGGTCGATCTTCCACAGTTAGAGATTTTGGCACTGCATGCTAA

Protein Analysis

152

Amino Acids

16.82

Weight (kDa)

8.93

Isoelectric Point (pI)

40.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_8 PF13855 70 - 130 1.7e-10 Leucine rich repeat
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 47, 261
AfaI GTAC 2 cut(s) 20, 397
AfiI CCNNNNNNNGG 2 cut(s) 53, 145
AgsI TTSAA 4 cut(s) 145, 218, 274, 361
Alw26I GTCTC 1 cut(s) 132
AoxI GGCC 2 cut(s) 3, 82
ApeKI GCWGC 1 cut(s) 263
ArsI GACNNNNNNTTYG 2 cut(s) 371, 403
AspLEI GCGC 2 cut(s) 138, 366
AspS9I GGNCC 3 cut(s) 82, 107, 201
AsuC2I CCSGG 1 cut(s) 34
AsuHPI GGTGA 1 cut(s) 160
AsuII TTCGAA 1 cut(s) 390
AvaII GGWCC 2 cut(s) 107, 201
AxyI CCTNAGG 1 cut(s) 86
BarI GAAGNNNNNNTAC 4 cut(s) 353, 384, 385, 416
BbvI GCAGC 1 cut(s) 250
BcnI CCSGG 1 cut(s) 34
BcoDI GTCTC 1 cut(s) 132
BfaI CTAG 1 cut(s) 53
BisI GCNGC 1 cut(s) 264
BlsI GCNGC 1 cut(s) 265
Bme1390I CCNGG 1 cut(s) 34
Bme18I GGWCC 2 cut(s) 107, 201
BmgT120I GGNCC 3 cut(s) 82, 107, 201
BmiI GGNNCC 3 cut(s) 109, 181, 202
BmrFI CCNGG 1 cut(s) 34
BmsI GCATC 1 cut(s) 58
Bpu14I TTCGAA 1 cut(s) 390
BpuMI CCSGG 1 cut(s) 34
BsaJI CCNNGG 1 cut(s) 33
BsaWI WCCGGW 1 cut(s) 403
Bsc4I CCNNNNNNNGG 2 cut(s) 53, 145
Bse118I RCCGGY 1 cut(s) 138
Bse21I CCTNAGG 1 cut(s) 86
BseDI CCNNGG 1 cut(s) 33
BseGI GGATG 1 cut(s) 412
BseLI CCNNNNNNNGG 2 cut(s) 53, 145
BseMII CTCAG 1 cut(s) 293
BseXI GCAGC 1 cut(s) 250
BsgI GTGCAG 1 cut(s) 267
BshFI GGCC 2 cut(s) 5, 84
BsiSI CCGG 3 cut(s) 33, 139, 404
BslFI GGGAC 1 cut(s) 93
BslI CCNNNNNNNGG 2 cut(s) 53, 145
BsmAI GTCTC 1 cut(s) 132
BsmFI GGGAC 1 cut(s) 93
BsnI GGCC 2 cut(s) 5, 84
Bsp119I TTCGAA 1 cut(s) 390
Bsp143I GATC 2 cut(s) 298, 419
BspACI CCGC 2 cut(s) 47, 261
BspANI GGCC 2 cut(s) 5, 84
BspCNI CTCAG 1 cut(s) 294
BspLI GGNNCC 3 cut(s) 109, 181, 202
BspT104I TTCGAA 1 cut(s) 390
BsrFI RCCGGY 1 cut(s) 138
BssAI RCCGGY 1 cut(s) 138
BssECI CCNNGG 1 cut(s) 33
BssMI GATC 2 cut(s) 298, 419
Bst4CI ACNGT 2 cut(s) 126, 430
BstAPI GCANNNNNTGC 1 cut(s) 450
BstBI TTCGAA 1 cut(s) 390
BstC8I GCNNGC 1 cut(s) 451
BstDEI CTNAG 2 cut(s) 86, 302
BstF5I GGATG 1 cut(s) 412
BstHHI GCGC 2 cut(s) 138, 366
BstKTI GATC 2 cut(s) 301, 422
BstMAI GTCTC 1 cut(s) 132
BstMBI GATC 2 cut(s) 298, 419
BstMWI GCNNNNNNNGC 1 cut(s) 450
BstNSI RCATGY 1 cut(s) 453
BstSCI CCNGG 1 cut(s) 32
BstV1I GCAGC 1 cut(s) 250
Bsu36I CCTNAGG 1 cut(s) 86
BsuRI GGCC 2 cut(s) 5, 84
BtsCI GGATG 1 cut(s) 412
BtsI GCAGTG 1 cut(s) 444
BtsIMutI CAGTG 1 cut(s) 444
Cac8I GCNNGC 1 cut(s) 451
CfoI GCGC 2 cut(s) 138, 366
Cfr10I RCCGGY 1 cut(s) 138
Cfr13I GGNCC 3 cut(s) 82, 107, 201
Csp6I GTAC 2 cut(s) 19, 396
CviAII CATG 3 cut(s) 22, 104, 450
CviJI RGCY 3 cut(s) 5, 84, 243
CviKI_1 RGCY 3 cut(s) 5, 84, 243
CviQI GTAC 2 cut(s) 19, 396
DdeI CTNAG 2 cut(s) 86, 302
DpnI GATC 2 cut(s) 300, 421
DpnII GATC 2 cut(s) 298, 419
Eco47I GGWCC 2 cut(s) 107, 201
Eco81I CCTNAGG 1 cut(s) 86
EcoO109I RGGNCCY 1 cut(s) 201
FaeI CATG 3 cut(s) 25, 107, 453
FaiI YATR 9 cut(s) 23, 105, 176, 227, 257, 329, 368, 370, 451
FaqI GGGAC 1 cut(s) 93
FatI CATG 3 cut(s) 21, 103, 449
FauI CCCGC 1 cut(s) 268
Fnu4HI GCNGC 1 cut(s) 264
FokI GGATG 1 cut(s) 419
Fsp4HI GCNGC 1 cut(s) 264
FspBI CTAG 1 cut(s) 53
GlaI GCGC 2 cut(s) 137, 365
GluI GCNGC 1 cut(s) 264
HaeIII GGCC 2 cut(s) 5, 84
HapII CCGG 3 cut(s) 33, 139, 404
HhaI GCGC 2 cut(s) 138, 366
Hin1II CATG 3 cut(s) 25, 107, 453
Hin6I GCGC 2 cut(s) 136, 364
HinP1I GCGC 2 cut(s) 136, 364
HpaII CCGG 3 cut(s) 33, 139, 404
HphI GGTGA 1 cut(s) 160
Hpy188I TCNGA 1 cut(s) 303
HpyAV CCTTC 5 cut(s) 139, 198, 214, 232, 386
HpyCH4III ACNGT 2 cut(s) 126, 430
HpyCH4V TGCA 2 cut(s) 284, 449
HpyF10VI GCNNNNNNNGC 1 cut(s) 450
HpyF3I CTNAG 2 cut(s) 86, 302
Hsp92II CATG 3 cut(s) 25, 107, 453
HspAI GCGC 2 cut(s) 136, 364
Kzo9I GATC 2 cut(s) 298, 419
LpnPI CCDG 6 cut(s) 46, 152, 234, 306, 346, 417
Lsp1109I GCAGC 1 cut(s) 250
LweI GCATC 1 cut(s) 58
MaeI CTAG 1 cut(s) 53
MalI GATC 2 cut(s) 300, 421
MboI GATC 2 cut(s) 298, 419
MboII GAAGA 2 cut(s) 262, 414
MnlI CCTC 2 cut(s) 54, 71
MseI TTAA 3 cut(s) 8, 153, 350
MspA1I CMGCKG 1 cut(s) 263
MspI CCGG 3 cut(s) 33, 139, 404
MspR9I CCNGG 1 cut(s) 34
MwoI GCNNNNNNNGC 1 cut(s) 450
NciI CCSGG 1 cut(s) 34
NdeII GATC 2 cut(s) 298, 419
NlaIII CATG 3 cut(s) 25, 107, 453
NlaIV GGNNCC 3 cut(s) 109, 181, 202
NspI RCATGY 1 cut(s) 453
NspV TTCGAA 1 cut(s) 390
PaeI GCATGC 1 cut(s) 453
PkrI GCNGC 1 cut(s) 265
PpuMI RGGWCCY 1 cut(s) 201
Psp5II RGGWCCY 1 cut(s) 201
PspN4I GGNNCC 3 cut(s) 109, 181, 202
PspPI GGNCC 3 cut(s) 82, 107, 201
PspPPI RGGWCCY 1 cut(s) 201
RsaI GTAC 2 cut(s) 20, 397
RsaNI GTAC 2 cut(s) 19, 396
SaqAI TTAA 3 cut(s) 8, 153, 350
SatI GCNGC 1 cut(s) 264
Sau3AI GATC 2 cut(s) 298, 419
Sau96I GGNCC 3 cut(s) 82, 107, 201
ScrFI CCNGG 1 cut(s) 34
SetI ASST 6 cut(s) 63, 150, 224, 325, 375, 397
SfaNI GCATC 1 cut(s) 58
SfuI TTCGAA 1 cut(s) 390
SinI GGWCC 2 cut(s) 107, 201
SphI GCATGC 1 cut(s) 453
SsiI CCGC 2 cut(s) 47, 261
SspMI CTAG 1 cut(s) 53
StyD4I CCNGG 1 cut(s) 32
TaaI ACNGT 2 cut(s) 126, 430
TaqI TCGA 4 cut(s) 160, 288, 390, 418
Tru1I TTAA 3 cut(s) 8, 153, 350
Tru9I TTAA 3 cut(s) 8, 153, 350
TscAI CASTG 1 cut(s) 451
TseI GCWGC 1 cut(s) 263
TspDTI ATGAA 3 cut(s) 92, 214, 244
TspRI CASTG 1 cut(s) 451
VpaK11BI GGWCC 2 cut(s) 107, 201
XceI RCATGY 1 cut(s) 453
XspI CTAG 1 cut(s) 53
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.