pycom03g13760

cysteine synthase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
N/A
Physical Location & Seq
Forward (+)
14859482 .. 14860021
540 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 231 bp
ATGTCCAGGTTTCTTATGAAGAACGATGGGCTCTTTTTGGGGAGTTCTTCAGCCATGAATTGTGTTGGAGCAGTCCGAGCGGCACAGGCACTTGGTCCCGGTCACACAATTGTGACCATTTTATGCGATAGTGGGATGAGACATTTGAGCAAATTTTTCAGCGCTGAGTATCTGTCTCAGTATGGTTTGACACCCTCGGCTAGCGGATTAGAGTTCCTAGGTGTTGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00270 ko00920 ko01100 ko01110 ko01120 ko01130 ko01200 ko01230 map00270 map00920 map01100 map01110 map01120 map01130 map01200 map01230
Pfam Domains
Protein Families
PALP

Protein Analysis

77

Amino Acids

7.97

Weight (kDa)

7.84

Isoelectric Point (pI)

27.41

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 80
AciI CCGC 2 cut(s) 80, 204
AcsI RAATTY 1 cut(s) 152
AcuI CTGAAG 1 cut(s) 33
AfeI AGCGCT 1 cut(s) 163
AjnI CCWGG 1 cut(s) 5
AleI CACNNNNGTG 1 cut(s) 110
Alw26I GTCTC 2 cut(s) 133, 180
Aor51HI AGCGCT 1 cut(s) 163
ApoI RAATTY 1 cut(s) 152
AspA2I CCTAGG 1 cut(s) 217
AspLEI GCGC 1 cut(s) 164
AspS9I GGNCC 1 cut(s) 95
AsuC2I CCSGG 1 cut(s) 99
AsuNHI GCTAGC 1 cut(s) 200
AvaII GGWCC 1 cut(s) 95
AvrII CCTAGG 1 cut(s) 217
BanII GRGCYC 1 cut(s) 33
BccI CCATC 1 cut(s) 20
BciT130I CCWGG 1 cut(s) 7
BcnI CCSGG 1 cut(s) 99
BcoDI GTCTC 2 cut(s) 133, 180
BfaI CTAG 2 cut(s) 201, 218
BfoI RGCGCY 1 cut(s) 165
BisI GCNGC 1 cut(s) 81
BlnI CCTAGG 1 cut(s) 217
BlsI GCNGC 1 cut(s) 82
Bme1390I CCNGG 2 cut(s) 7, 99
Bme18I GGWCC 1 cut(s) 95
BmgT120I GGNCC 1 cut(s) 95
BmiI GGNNCC 1 cut(s) 97
BmrFI CCNGG 2 cut(s) 7, 99
BmtI GCTAGC 1 cut(s) 204
BpuMI CCSGG 1 cut(s) 99
BsaJI CCNNGG 2 cut(s) 195, 217
BseBI CCWGG 1 cut(s) 7
BseDI CCNNGG 2 cut(s) 195, 217
BseGI GGATG 1 cut(s) 141
BseMII CTCAG 2 cut(s) 156, 191
BsiSI CCGG 1 cut(s) 99
BslFI GGGAC 1 cut(s) 81
BsmAI GTCTC 2 cut(s) 133, 180
BsmFI GGGAC 1 cut(s) 81
Bsp1286I GDGCHC 1 cut(s) 33
BspACI CCGC 2 cut(s) 80, 204
BspCNI CTCAG 2 cut(s) 157, 190
BspLI GGNNCC 1 cut(s) 97
BspOI GCTAGC 1 cut(s) 204
BsrBI CCGCTC 1 cut(s) 80
BssECI CCNNGG 2 cut(s) 195, 217
BssT1I CCWWGG 1 cut(s) 217
Bst2UI CCWGG 1 cut(s) 7
BstC8I GCNNGC 1 cut(s) 202
BstDEI CTNAG 2 cut(s) 165, 177
BstF5I GGATG 1 cut(s) 141
BstH2I RGCGCY 1 cut(s) 165
BstHHI GCGC 1 cut(s) 164
BstMAI GTCTC 2 cut(s) 133, 180
BstMWI GCNNNNNNNGC 2 cut(s) 77, 86
BstNI CCWGG 1 cut(s) 7
BstSCI CCNGG 2 cut(s) 5, 97
BtsCI GGATG 1 cut(s) 141
Cac8I GCNNGC 1 cut(s) 202
CfoI GCGC 1 cut(s) 164
Cfr13I GGNCC 1 cut(s) 95
CviAII CATG 1 cut(s) 55
CviJI RGCY 3 cut(s) 31, 53, 200
CviKI_1 RGCY 3 cut(s) 31, 53, 200
DdeI CTNAG 2 cut(s) 165, 177
Eco130I CCWWGG 1 cut(s) 217
Eco24I GRGCYC 1 cut(s) 33
Eco47I GGWCC 1 cut(s) 95
Eco47III AGCGCT 1 cut(s) 163
Eco57I CTGAAG 1 cut(s) 33
EcoRII CCWGG 1 cut(s) 5
EcoT14I CCWWGG 1 cut(s) 217
EcoT38I GRGCYC 1 cut(s) 33
ErhI CCWWGG 1 cut(s) 217
FaeI CATG 1 cut(s) 58
FaiI YATR 4 cut(s) 17, 56, 124, 183
FaqI GGGAC 1 cut(s) 81
FatI CATG 1 cut(s) 54
Fnu4HI GCNGC 1 cut(s) 81
FokI GGATG 1 cut(s) 148
FriOI GRGCYC 1 cut(s) 33
Fsp4HI GCNGC 1 cut(s) 81
FspBI CTAG 2 cut(s) 201, 218
GlaI GCGC 1 cut(s) 163
GluI GCNGC 1 cut(s) 81
HaeII RGCGCY 1 cut(s) 165
HapII CCGG 1 cut(s) 99
HhaI GCGC 1 cut(s) 164
Hin1II CATG 1 cut(s) 58
Hin6I GCGC 1 cut(s) 162
HinP1I GCGC 1 cut(s) 162
HpaII CCGG 1 cut(s) 99
Hpy188I TCNGA 1 cut(s) 77
HpyF10VI GCNNNNNNNGC 2 cut(s) 77, 86
HpyF3I CTNAG 2 cut(s) 165, 177
Hsp92II CATG 1 cut(s) 58
HspAI GCGC 1 cut(s) 162
LmnI GCTCC 1 cut(s) 68
LpnPI CCDG 3 cut(s) 19, 71, 112
MaeI CTAG 2 cut(s) 201, 218
MaeIII GTNAC 2 cut(s) 101, 112
MbiI CCGCTC 1 cut(s) 80
MboII GAAGA 2 cut(s) 31, 39
MfeI CAATTG 1 cut(s) 108
MhlI GDGCHC 1 cut(s) 33
MluCI AATT 3 cut(s) 58, 108, 152
MmeI TCCRAC 2 cut(s) 46, 205
MnlI CCTC 1 cut(s) 205
MslI CAYNNNNRTG 1 cut(s) 110
MspI CCGG 1 cut(s) 99
MspR9I CCNGG 2 cut(s) 7, 99
MunI CAATTG 1 cut(s) 108
MvaI CCWGG 1 cut(s) 7
MwoI GCNNNNNNNGC 2 cut(s) 77, 86
NciI CCSGG 1 cut(s) 99
NheI GCTAGC 1 cut(s) 200
NlaIII CATG 1 cut(s) 58
NlaIV GGNNCC 1 cut(s) 97
NmeAIII GCCGAG 1 cut(s) 176
NmuCI GTSAC 2 cut(s) 101, 112
OliI CACNNNNGTG 1 cut(s) 110
PkrI GCNGC 1 cut(s) 82
Psp6I CCWGG 1 cut(s) 5
PspGI CCWGG 1 cut(s) 5
PspN4I GGNNCC 1 cut(s) 97
PspPI GGNCC 1 cut(s) 95
RseI CAYNNNNRTG 1 cut(s) 110
SatI GCNGC 1 cut(s) 81
Sau96I GGNCC 1 cut(s) 95
ScrFI CCNGG 2 cut(s) 7, 99
SduI GDGCHC 1 cut(s) 33
SetI ASST 2 cut(s) 11, 223
SinI GGWCC 1 cut(s) 95
SmiMI CAYNNNNRTG 1 cut(s) 110
Sse9I AATT 3 cut(s) 58, 108, 152
SsiI CCGC 2 cut(s) 80, 204
SspMI CTAG 2 cut(s) 201, 218
StyD4I CCNGG 2 cut(s) 5, 97
StyI CCWWGG 1 cut(s) 217
TasI AATT 3 cut(s) 58, 108, 152
TauI GCSGC 1 cut(s) 83
TseFI GTSAC 2 cut(s) 101, 112
Tsp45I GTSAC 2 cut(s) 101, 112
TspDTI ATGAA 2 cut(s) 32, 71
VpaK11BI GGWCC 1 cut(s) 95
XapI RAATTY 1 cut(s) 152
XmaJI CCTAGG 1 cut(s) 217
XspI CTAG 2 cut(s) 201, 218
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.