pycom03g14620

Belongs to the 'GDSL' lipolytic enzyme family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
N/A
Physical Location & Seq
Forward (+)
15949252 .. 15950022
771 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 303 bp
ATGGCTCCTGCTTGCTCATCTCCTAGTGCAGATTCTATGGTGTGTGCGAAAGTTCCAGCAATCATTGTGTTTGGAGACTACTGTGGATACAGGGAACAACAACCAGATATCAACAGTTTTAAAGATAAGACTTTGAACCCTACGGTCGCGATTTCTATGGAGGCTGGCCTACTGGCCGGCAGAGCGTCGGAGTTTTCCATTGAGGAGCACCAAAACTTTCTACTAGGAATTGCTGGAAATTTCAACAGGGCGCTTTACCATCTTGGAGATCGGGAGATGTCTCGGGCGGCCGTCCTCCAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Lipase_GDSL

Protein Analysis

101

Amino Acids

10.83

Weight (kDa)

5.19

Isoelectric Point (pI)

44.88

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 149
AciI CCGC 1 cut(s) 287
AcoI YGGCCR 2 cut(s) 174, 288
AcsI RAATTY 1 cut(s) 238
AgsI TTSAA 2 cut(s) 136, 244
Alw21I GWGCWC 1 cut(s) 210
Alw26I GTCTC 2 cut(s) 69, 285
Ama87I CYCGRG 1 cut(s) 282
AoxI GGCC 3 cut(s) 166, 174, 288
ApoI RAATTY 1 cut(s) 238
AspLEI GCGC 1 cut(s) 253
AvaI CYCGRG 1 cut(s) 282
Bbv12I GWGCWC 1 cut(s) 210
BccI CCATC 1 cut(s) 267
BceAI ACGGC 1 cut(s) 275
BciVI GTATCC 1 cut(s) 80
BcoDI GTCTC 2 cut(s) 69, 285
BfaI CTAG 2 cut(s) 24, 224
BfoI RGCGCY 1 cut(s) 254
BfuI GTATCC 1 cut(s) 80
BisI GCNGC 1 cut(s) 288
BlsI GCNGC 1 cut(s) 289
BmeT110I CYCGRG 1 cut(s) 282
BmiI GGNNCC 1 cut(s) 6
Bse118I RCCGGY 1 cut(s) 176
Bse1I ACTGG 1 cut(s) 177
BseNI ACTGG 1 cut(s) 177
BseRI GAGGAG 1 cut(s) 218
BseX3I CGGCCG 1 cut(s) 288
BsgI GTGCAG 1 cut(s) 48
Bsh1236I CGCG 1 cut(s) 149
Bsh1285I CGRYCG 2 cut(s) 147, 291
BshFI GGCC 3 cut(s) 168, 176, 290
BsiEI CGRYCG 2 cut(s) 147, 291
BsiHKAI GWGCWC 1 cut(s) 210
BsiHKCI CYCGRG 1 cut(s) 282
BsiSI CCGG 1 cut(s) 177
BsmAI GTCTC 2 cut(s) 69, 285
BsnI GGCC 3 cut(s) 168, 176, 290
BsoBI CYCGRG 1 cut(s) 282
Bsp1286I GDGCHC 1 cut(s) 210
Bsp143I GATC 1 cut(s) 268
Bsp68I TCGCGA 1 cut(s) 149
BspACI CCGC 1 cut(s) 287
BspANI GGCC 3 cut(s) 168, 176, 290
BspFNI CGCG 1 cut(s) 149
BspLI GGNNCC 1 cut(s) 6
BsrFI RCCGGY 1 cut(s) 176
BsrI ACTGG 1 cut(s) 177
BssAI RCCGGY 1 cut(s) 176
BssMI GATC 1 cut(s) 268
Bst4CI ACNGT 3 cut(s) 83, 116, 145
BstC8I GCNNGC 3 cut(s) 13, 166, 178
BstFNI CGCG 1 cut(s) 149
BstH2I RGCGCY 1 cut(s) 254
BstHHI GCGC 1 cut(s) 253
BstKTI GATC 1 cut(s) 271
BstMAI GTCTC 2 cut(s) 69, 285
BstMBI GATC 1 cut(s) 268
BstMCI CGRYCG 2 cut(s) 147, 291
BstMWI GCNNNNNNNGC 1 cut(s) 182
BstUI CGCG 1 cut(s) 149
BstZI CGGCCG 1 cut(s) 288
BsuI GTATCC 1 cut(s) 80
BsuRI GGCC 3 cut(s) 168, 176, 290
BtuMI TCGCGA 1 cut(s) 149
Cac8I GCNNGC 3 cut(s) 13, 166, 178
CfoI GCGC 1 cut(s) 253
Cfr10I RCCGGY 1 cut(s) 176
CseI GACGC 1 cut(s) 174
CviJI RGCY 5 cut(s) 5, 164, 168, 176, 290
CviKI_1 RGCY 5 cut(s) 5, 164, 168, 176, 290
DpnI GATC 1 cut(s) 270
DpnII GATC 1 cut(s) 268
DraI TTTAAA 1 cut(s) 121
EaeI YGGCCR 2 cut(s) 174, 288
EagI CGGCCG 1 cut(s) 288
EclXI CGGCCG 1 cut(s) 288
Eco32I GATATC 1 cut(s) 109
Eco52I CGGCCG 1 cut(s) 288
Eco88I CYCGRG 1 cut(s) 282
EcoRV GATATC 1 cut(s) 109
FaiI YATR 2 cut(s) 38, 158
Fnu4HI GCNGC 1 cut(s) 288
Fsp4HI GCNGC 1 cut(s) 288
FspBI CTAG 2 cut(s) 24, 224
GlaI GCGC 1 cut(s) 252
GluI GCNGC 1 cut(s) 288
HaeII RGCGCY 1 cut(s) 254
HaeIII GGCC 3 cut(s) 168, 176, 290
HapII CCGG 1 cut(s) 177
HgaI GACGC 1 cut(s) 174
HhaI GCGC 1 cut(s) 253
Hin6I GCGC 1 cut(s) 251
HinP1I GCGC 1 cut(s) 251
HinfI GANTC 1 cut(s) 32
HpaII CCGG 1 cut(s) 177
Hpy188I TCNGA 1 cut(s) 190
Hpy188III TCNNGA 2 cut(s) 148, 272
Hpy99I CGWCG 1 cut(s) 190
HpyCH4III ACNGT 3 cut(s) 83, 116, 145
HpyCH4V TGCA 1 cut(s) 29
HpyF10VI GCNNNNNNNGC 1 cut(s) 182
HspAI GCGC 1 cut(s) 251
KroI GCCGGC 1 cut(s) 176
KroNI GCCGGC 1 cut(s) 178
Kzo9I GATC 1 cut(s) 268
LmnI GCTCC 2 cut(s) 10, 205
LpnPI CCDG 9 cut(s) 21, 69, 76, 117, 150, 158, 190, 219, 232
MaeI CTAG 2 cut(s) 24, 224
MalI GATC 1 cut(s) 270
MboI GATC 1 cut(s) 268
MhlI GDGCHC 1 cut(s) 210
MluCI AATT 2 cut(s) 228, 238
MmeI TCCRAC 1 cut(s) 168
MnlI CCTC 2 cut(s) 154, 196
MroNI GCCGGC 1 cut(s) 176
MseI TTAA 1 cut(s) 120
MspI CCGG 1 cut(s) 177
MvnI CGCG 1 cut(s) 149
MwoI GCNNNNNNNGC 1 cut(s) 182
NaeI GCCGGC 1 cut(s) 178
NdeII GATC 1 cut(s) 268
NgoMIV GCCGGC 1 cut(s) 176
NlaIV GGNNCC 1 cut(s) 6
NruI TCGCGA 1 cut(s) 149
PdiI GCCGGC 1 cut(s) 178
PfeI GAWTC 1 cut(s) 32
PkrI GCNGC 1 cut(s) 289
PspN4I GGNNCC 1 cut(s) 6
RruI TCGCGA 1 cut(s) 149
SaqAI TTAA 1 cut(s) 120
SatI GCNGC 1 cut(s) 288
Sau3AI GATC 1 cut(s) 268
SduI GDGCHC 1 cut(s) 210
Sse9I AATT 2 cut(s) 228, 238
SsiI CCGC 1 cut(s) 287
SspMI CTAG 2 cut(s) 24, 224
TaaI ACNGT 3 cut(s) 83, 116, 145
TasI AATT 2 cut(s) 228, 238
TauI GCSGC 1 cut(s) 290
TfiI GAWTC 1 cut(s) 32
Tru1I TTAA 1 cut(s) 120
Tru9I TTAA 1 cut(s) 120
XapI RAATTY 1 cut(s) 238
XspI CTAG 2 cut(s) 24, 224
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.