pycom03g18390

At5g64816-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
N/A
Physical Location & Seq
Forward (+)
19363842 .. 19365509
1668 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 747 bp
ATGCCTGATGAACATTTCGGGATTCTTGTAGCAATCTTTCCTGTTACTGTTTGTGTATCAGATGATATGAGTCTGCTGCTGAAGAAGAAGTTGACCCCTGCTTTTGTGATGGTGGAAGTGGGGTGGTCCCTCTTAGCTGCTATCCCTGCAGTGGTTGCAGGACAAGCATTTCCAGTGAAGAAAATGCGCGCCGAAGAGCAGAGATTAAAGGGCGCTAGAGGAAGGGAGAAGAGTTCTGATGAGATTTTTGTCTGCGAAAGGGTATGCACGTCGATGAGAATGCTGAAAAAGGTTGGTGCATTCTCAAAGGACCCAATTCATGATACTTATGTTACCGTCTGCGGCGTATCTGAGGTTGATGCGTGTGCTGATGCATGCGCACGCACTGTCTGTGTAAACCAACATCAAGTACCCAATTGGAATGACATCTGCCTTCGGAGATGCCAGAGTGAGTGCTTGAACTCTCTTCTTCAAGTTCTCTTTAAATGCGAAAACATGTATTACAAAACGAGTTATGTTAAAAAGGAACTCAGGACCAATACCATCCCAACTAAAAGAACCTCTAATTTTTTTAATAAATGTTGCAACAGAATGTGCCGCTCAGTTGCCTTCTCGACGAACAATCTCGAACGAAACAGTCCCCACATCATTTGTAAAGCCCAAATGTCATTAACGATGCTTTCCACCTCCGCATCAACACCGATCTCCTTTCACGTTTTTGTTTTCTTTTTTCCTTTTTGTTGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

249

Amino Acids

27.95

Weight (kDa)

8.9

Isoelectric Point (pI)

42.26

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 379
AccBSI CCGCTC 1 cut(s) 600
AccII CGCG 1 cut(s) 189
AciI CCGC 3 cut(s) 342, 598, 690
AcuI CTGAAG 1 cut(s) 101
AfaI GTAC 1 cut(s) 411
AfiI CCNNNNNNNGG 1 cut(s) 151
AflIII ACRYGT 1 cut(s) 495
AgsI TTSAA 2 cut(s) 460, 473
AjiI CACGTC 1 cut(s) 270
AluBI AGCT 1 cut(s) 137
AluI AGCT 1 cut(s) 137
ApeKI GCWGC 2 cut(s) 76, 137
AspLEI GCGC 4 cut(s) 189, 191, 215, 380
AspS9I GGNCC 3 cut(s) 126, 310, 534
AvaII GGWCC 3 cut(s) 126, 310, 534
BaeI ACNNNNGTAYC 2 cut(s) 315, 348
BbvI GCAGC 2 cut(s) 63, 124
BccI CCATC 2 cut(s) 103, 551
BcgI CGANNNNNNTGC 2 cut(s) 262, 296
BfaI CTAG 1 cut(s) 216
BfmI CTRYAG 1 cut(s) 147
BfoI RGCGCY 1 cut(s) 216
BisI GCNGC 4 cut(s) 77, 138, 343, 598
BlsI GCNGC 4 cut(s) 78, 139, 344, 599
Bme18I GGWCC 3 cut(s) 126, 310, 534
BmgBI CACGTC 1 cut(s) 270
BmgT120I GGNCC 3 cut(s) 126, 310, 534
BmiI GGNNCC 2 cut(s) 128, 312
BmsI GCATC 5 cut(s) 349, 361, 431, 666, 701
Bsc4I CCNNNNNNNGG 1 cut(s) 151
Bse1I ACTGG 1 cut(s) 173
BseGI GGATG 1 cut(s) 543
BseLI CCNNNNNNNGG 1 cut(s) 151
BseMII CTCAG 3 cut(s) 342, 544, 615
BseNI ACTGG 1 cut(s) 173
BsePI GCGCGC 1 cut(s) 187
BseXI GCAGC 2 cut(s) 63, 124
Bsh1236I CGCG 1 cut(s) 189
BslFI GGGAC 2 cut(s) 112, 624
BslI CCNNNNNNNGG 1 cut(s) 151
BsmFI GGGAC 2 cut(s) 112, 624
BsmI GAATGC 2 cut(s) 285, 299
Bsp143I GATC 1 cut(s) 702
BspACI CCGC 3 cut(s) 342, 598, 690
BspCNI CTCAG 3 cut(s) 343, 543, 614
BspFNI CGCG 1 cut(s) 189
BspHI TCATGA 1 cut(s) 319
BspLI GGNNCC 2 cut(s) 128, 312
BspMAI CTGCAG 1 cut(s) 151
BspQI GCTCTTC 1 cut(s) 189
BsrBI CCGCTC 1 cut(s) 600
BsrI ACTGG 1 cut(s) 173
BssHII GCGCGC 1 cut(s) 187
BssMI GATC 1 cut(s) 702
Bst4CI ACNGT 4 cut(s) 49, 337, 388, 638
Bst6I CTCTTC 3 cut(s) 189, 224, 471
BstAPI GCANNNNNTGC 1 cut(s) 155
BstC8I GCNNGC 3 cut(s) 189, 376, 382
BstDEI CTNAG 4 cut(s) 133, 351, 530, 601
BstF5I GGATG 1 cut(s) 543
BstFNI CGCG 1 cut(s) 189
BstH2I RGCGCY 1 cut(s) 216
BstHHI GCGC 4 cut(s) 189, 191, 215, 380
BstKTI GATC 1 cut(s) 705
BstMBI GATC 1 cut(s) 702
BstMWI GCNNNNNNNGC 3 cut(s) 146, 155, 164
BstNSI RCATGY 2 cut(s) 378, 499
BstSFI CTRYAG 1 cut(s) 147
BstUI CGCG 1 cut(s) 189
BstV1I GCAGC 2 cut(s) 63, 124
BtrI CACGTC 1 cut(s) 270
BtsCI GGATG 1 cut(s) 543
BtsI GCAGTG 1 cut(s) 156
BtsIMutI CAGTG 3 cut(s) 156, 180, 384
Cac8I GCNNGC 3 cut(s) 189, 376, 382
CciI TCATGA 1 cut(s) 319
CfoI GCGC 4 cut(s) 189, 191, 215, 380
Cfr13I GGNCC 3 cut(s) 126, 310, 534
Csp6I GTAC 1 cut(s) 410
CspCI CAANNNNNGTGG 2 cut(s) 632, 667
CviAII CATG 3 cut(s) 320, 375, 496
CviJI RGCY 2 cut(s) 137, 659
CviKI_1 RGCY 2 cut(s) 137, 659
CviQI GTAC 1 cut(s) 410
DdeI CTNAG 4 cut(s) 133, 351, 530, 601
DpnI GATC 1 cut(s) 704
DpnII GATC 1 cut(s) 702
DraI TTTAAA 1 cut(s) 484
Eam1104I CTCTTC 3 cut(s) 189, 224, 471
EarI CTCTTC 3 cut(s) 189, 224, 471
Eco47I GGWCC 3 cut(s) 126, 310, 534
Eco57I CTGAAG 1 cut(s) 101
EcoO109I RGGNCCY 1 cut(s) 310
EcoT22I ATGCAT 1 cut(s) 376
FaeI CATG 3 cut(s) 323, 378, 499
FaiI YATR 7 cut(s) 68, 265, 321, 330, 376, 497, 516
FaqI GGGAC 2 cut(s) 112, 624
FatI CATG 3 cut(s) 319, 374, 495
Fnu4HI GCNGC 4 cut(s) 77, 138, 343, 598
FokI GGATG 1 cut(s) 530
Fsp4HI GCNGC 4 cut(s) 77, 138, 343, 598
FspAI RTGCGCAY 1 cut(s) 379
FspBI CTAG 1 cut(s) 216
FspI TGCGCA 1 cut(s) 379
GlaI GCGC 4 cut(s) 188, 190, 214, 379
GluI GCNGC 4 cut(s) 77, 138, 343, 598
HaeII RGCGCY 1 cut(s) 216
HhaI GCGC 4 cut(s) 189, 191, 215, 380
Hin1II CATG 3 cut(s) 323, 378, 499
Hin6I GCGC 4 cut(s) 187, 189, 213, 378
HinP1I GCGC 4 cut(s) 187, 189, 213, 378
HincII GTYRAC 1 cut(s) 93
HindII GTYRAC 1 cut(s) 93
HinfI GANTC 2 cut(s) 22, 70
Hpy166II GTNNAC 2 cut(s) 93, 397
Hpy188I TCNGA 4 cut(s) 61, 238, 352, 438
Hpy188III TCNNGA 5 cut(s) 19, 320, 532, 613, 626
Hpy8I GTNNAC 2 cut(s) 93, 397
Hpy99I CGWCG 2 cut(s) 274, 619
HpyAV CCTTC 3 cut(s) 216, 443, 619
HpyCH4III ACNGT 4 cut(s) 49, 337, 388, 638
HpyCH4IV ACGT 2 cut(s) 269, 714
HpyCH4V TGCA 6 cut(s) 149, 158, 267, 299, 374, 585
HpyF10VI GCNNNNNNNGC 3 cut(s) 146, 155, 164
HpyF3I CTNAG 4 cut(s) 133, 351, 530, 601
HpySE526I ACGT 2 cut(s) 269, 714
Hsp92II CATG 3 cut(s) 323, 378, 499
HspAI GCGC 4 cut(s) 187, 189, 213, 378
Kzo9I GATC 1 cut(s) 702
LguI GCTCTTC 1 cut(s) 189
LpnPI CCDG 8 cut(s) 18, 54, 111, 144, 159, 186, 458, 517
Lsp1109I GCAGC 2 cut(s) 63, 124
LweI GCATC 5 cut(s) 349, 361, 431, 666, 701
MaeI CTAG 1 cut(s) 216
MaeII ACGT 2 cut(s) 269, 714
MaeIII GTNAC 2 cut(s) 43, 331
MalI GATC 1 cut(s) 704
MbiI CCGCTC 1 cut(s) 600
MboI GATC 1 cut(s) 702
MboII GAAGA 7 cut(s) 94, 97, 190, 206, 241, 458, 461
MfeI CAATTG 1 cut(s) 415
MluCI AATT 3 cut(s) 315, 415, 565
MlyI GAGTC 1 cut(s) 79
MnlI CCTC 5 cut(s) 140, 212, 346, 571, 697
Mph1103I ATGCAT 1 cut(s) 376
MseI TTAA 5 cut(s) 206, 483, 519, 573, 671
MslI CAYNNNNRTG 1 cut(s) 272
MunI CAATTG 1 cut(s) 415
Mva1269I GAATGC 2 cut(s) 285, 299
MvnI CGCG 1 cut(s) 189
MwoI GCNNNNNNNGC 3 cut(s) 146, 155, 164
NdeII GATC 1 cut(s) 702
NlaIII CATG 3 cut(s) 323, 378, 499
NlaIV GGNNCC 2 cut(s) 128, 312
NsbI TGCGCA 1 cut(s) 379
NsiI ATGCAT 1 cut(s) 376
NspI RCATGY 2 cut(s) 378, 499
PaeI GCATGC 1 cut(s) 378
PagI TCATGA 1 cut(s) 319
PauI GCGCGC 1 cut(s) 187
PciI ACATGT 1 cut(s) 495
PciSI GCTCTTC 1 cut(s) 189
PctI GAATGC 2 cut(s) 285, 299
PfeI GAWTC 1 cut(s) 22
PkrI GCNGC 4 cut(s) 78, 139, 344, 599
PleI GAGTC 1 cut(s) 78
PpsI GAGTC 1 cut(s) 78
PpuMI RGGWCCY 1 cut(s) 310
PscI ACATGT 1 cut(s) 495
Psp5II RGGWCCY 1 cut(s) 310
PspN4I GGNNCC 2 cut(s) 128, 312
PspPI GGNCC 3 cut(s) 126, 310, 534
PspPPI RGGWCCY 1 cut(s) 310
PstI CTGCAG 1 cut(s) 151
PteI GCGCGC 1 cut(s) 187
RsaI GTAC 1 cut(s) 411
RsaNI GTAC 1 cut(s) 410
RseI CAYNNNNRTG 1 cut(s) 272
SapI GCTCTTC 1 cut(s) 189
SaqAI TTAA 5 cut(s) 206, 483, 519, 573, 671
SatI GCNGC 4 cut(s) 77, 138, 343, 598
Sau3AI GATC 1 cut(s) 702
Sau96I GGNCC 3 cut(s) 126, 310, 534
SchI GAGTC 1 cut(s) 79
SetI ASST 7 cut(s) 139, 272, 294, 357, 563, 689, 717
SfaNI GCATC 5 cut(s) 349, 361, 431, 666, 701
SfcI CTRYAG 1 cut(s) 147
SinI GGWCC 3 cut(s) 126, 310, 534
SmiMI CAYNNNNRTG 1 cut(s) 272
SphI GCATGC 1 cut(s) 378
Sse9I AATT 3 cut(s) 315, 415, 565
SsiI CCGC 3 cut(s) 342, 598, 690
SspMI CTAG 1 cut(s) 216
TaaI ACNGT 4 cut(s) 49, 337, 388, 638
TaiI ACGT 2 cut(s) 272, 717
TaqI TCGA 3 cut(s) 272, 614, 627
TasI AATT 3 cut(s) 315, 415, 565
TauI GCSGC 2 cut(s) 345, 600
TfiI GAWTC 1 cut(s) 22
Tru1I TTAA 5 cut(s) 206, 483, 519, 573, 671
Tru9I TTAA 5 cut(s) 206, 483, 519, 573, 671
TscAI CASTG 3 cut(s) 156, 180, 391
TseI GCWGC 2 cut(s) 76, 137
TspDTI ATGAA 2 cut(s) 24, 308
TspRI CASTG 3 cut(s) 156, 180, 391
VpaK11BI GGWCC 3 cut(s) 126, 310, 534
XceI RCATGY 2 cut(s) 378, 499
XspI CTAG 1 cut(s) 216
Zsp2I ATGCAT 1 cut(s) 376
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.