pycom03g18670

Topless-related protein 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
N/A
Physical Location & Seq
Forward (+)
19594862 .. 19596067
1206 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 783 bp
ATGAAAGAACACATTCTTGAAGATTGTTCACACGGAGAATGTTACTCTGCTGATGAGAGAGAGCCAAAACAATTCTTAGTTAATCTCAGCCCTCCATTCCGTCTAGCCTATGAGCTAAATACAAGTGTCCTATCGAATTACAGTTGGAATTCAAACATAAACAGCGAACACATTTATATTTCAACAGTTTATTGTTGGGAGGGGTTCATGGCCCAGAATTGCAGTTGCTTGGCGGGGGATTATTGTAGTTGTTTGATGGACCCATGTTGGGAGGCGGCATTGGAGCCCTGTTCGGGGGTGGAGGACCCATGCTGGCAGATTCAGGTCCCGAATTTGCACAATTCTGCATATTTTCCCTTCTCCTTCCAAAGTTACGAGGTCTGTCATTACGCCTATTTCTCTCATTTTCTTGACTATTGTTTCTTAGCAAGAAGAAAAGGAGAGACAGAGATGGAAAAAAAAAAAAAATTAATGCAGAGACTTGTGAGGATAACAAAGGCACAAAACGTAGTTCAGACTTGTGAGCGAATCAAGCAGAGAACAATAGAAGCCTTAAAAGACCCCAAGGTGAAGTCATGTTTTTCACTGGACAGAAAGACTTTAATAGATGGTCAAGTTGCTGCTCAACGGGACATAAGCATGTCACATAACTCACCTTTGGAGAAACTGGATCTAGAGCTTGTAAAGAGAGAAAATTCGAAAAGAAGTTTTGTTAGCAGACCAGACCAGAGCTGCCAAAAACAGAAAAATGGAAGTAGCTGTCGTAACCTCATCCTCTGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04934 map04934
Pfam Domains
Protein Families
WD40

Protein Analysis

261

Amino Acids

30.11

Weight (kDa)

6.99

Isoelectric Point (pI)

56.17

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 233, 275
AclWI GGATC 1 cut(s) 678
AcsI RAATTY 3 cut(s) 148, 331, 694
AfiI CCNNNNNNNGG 3 cut(s) 268, 293, 294
AgsI TTSAA 3 cut(s) 20, 153, 183
AluBI AGCT 4 cut(s) 115, 679, 732, 759
AluI AGCT 4 cut(s) 115, 679, 732, 759
Alw26I GTCTC 2 cut(s) 437, 472
AlwI GGATC 1 cut(s) 678
AoxI GGCC 1 cut(s) 210
ApeKI GCWGC 2 cut(s) 620, 732
ApoI RAATTY 3 cut(s) 148, 331, 694
AseI ATTAAT 1 cut(s) 470
Asp700I GAANNNNTTC 1 cut(s) 12
AspS9I GGNCC 4 cut(s) 211, 259, 304, 325
AsuHPI GGTGA 2 cut(s) 580, 645
AsuII TTCGAA 1 cut(s) 698
AvaII GGWCC 3 cut(s) 259, 304, 325
BanII GRGCYC 1 cut(s) 288
BbvI GCAGC 2 cut(s) 607, 719
BccI CCATC 3 cut(s) 250, 445, 602
BcoDI GTCTC 2 cut(s) 437, 472
BfaI CTAG 2 cut(s) 104, 674
BisI GCNGC 3 cut(s) 276, 621, 733
BlsI GCNGC 3 cut(s) 277, 622, 734
Bme18I GGWCC 3 cut(s) 259, 304, 325
BmgT120I GGNCC 4 cut(s) 211, 259, 304, 325
BmiI GGNNCC 4 cut(s) 261, 285, 306, 327
Bpu14I TTCGAA 1 cut(s) 698
BsaJI CCNNGG 1 cut(s) 564
Bsc4I CCNNNNNNNGG 3 cut(s) 268, 293, 294
Bse1I ACTGG 2 cut(s) 591, 672
BseDI CCNNGG 1 cut(s) 564
BseGI GGATG 1 cut(s) 771
BseLI CCNNNNNNNGG 3 cut(s) 268, 293, 294
BseMII CTCAG 1 cut(s) 100
BseNI ACTGG 2 cut(s) 591, 672
BseXI GCAGC 2 cut(s) 607, 719
BshFI GGCC 1 cut(s) 212
BslFI GGGAC 2 cut(s) 311, 644
BslI CCNNNNNNNGG 3 cut(s) 268, 293, 294
BsmAI GTCTC 2 cut(s) 437, 472
BsmFI GGGAC 2 cut(s) 311, 644
BsnI GGCC 1 cut(s) 212
Bsp119I TTCGAA 1 cut(s) 698
Bsp1286I GDGCHC 1 cut(s) 288
Bsp143I GATC 1 cut(s) 670
BspACI CCGC 2 cut(s) 233, 275
BspANI GGCC 1 cut(s) 212
BspCNI CTCAG 1 cut(s) 99
BspLI GGNNCC 4 cut(s) 261, 285, 306, 327
BspPI GGATC 1 cut(s) 678
BspT104I TTCGAA 1 cut(s) 698
BsrI ACTGG 2 cut(s) 591, 672
BssECI CCNNGG 1 cut(s) 564
BssMI GATC 1 cut(s) 670
BssT1I CCWWGG 1 cut(s) 564
Bst4CI ACNGT 2 cut(s) 143, 187
BstBI TTCGAA 1 cut(s) 698
BstC8I GCNNGC 1 cut(s) 314
BstDEI CTNAG 3 cut(s) 76, 86, 424
BstF5I GGATG 1 cut(s) 771
BstKTI GATC 1 cut(s) 673
BstMAI GTCTC 2 cut(s) 437, 472
BstMBI GATC 1 cut(s) 670
BstMWI GCNNNNNNNGC 1 cut(s) 532
BstNSI RCATGY 1 cut(s) 643
BstV1I GCAGC 2 cut(s) 607, 719
BstX2I RGATCY 1 cut(s) 670
BstYI RGATCY 1 cut(s) 670
BsuRI GGCC 1 cut(s) 212
BtsCI GGATG 1 cut(s) 771
BtsIMutI CAGTG 1 cut(s) 584
Cac8I GCNNGC 1 cut(s) 314
Cfr13I GGNCC 4 cut(s) 211, 259, 304, 325
CviAII CATG 5 cut(s) 208, 264, 309, 576, 640
DdeI CTNAG 3 cut(s) 76, 86, 424
DpnI GATC 1 cut(s) 672
DpnII GATC 1 cut(s) 670
Eco130I CCWWGG 1 cut(s) 564
Eco24I GRGCYC 1 cut(s) 288
Eco47I GGWCC 3 cut(s) 259, 304, 325
EcoO109I RGGNCCY 2 cut(s) 304, 325
EcoRI GAATTC 1 cut(s) 148
EcoT14I CCWWGG 1 cut(s) 564
EcoT38I GRGCYC 1 cut(s) 288
ErhI CCWWGG 1 cut(s) 564
FaeI CATG 5 cut(s) 211, 267, 312, 579, 643
FaqI GGGAC 2 cut(s) 311, 644
FatI CATG 5 cut(s) 207, 263, 308, 575, 639
FauI CCCGC 1 cut(s) 226
Fnu4HI GCNGC 3 cut(s) 276, 621, 733
FokI GGATG 1 cut(s) 758
FriOI GRGCYC 1 cut(s) 288
Fsp4HI GCNGC 3 cut(s) 276, 621, 733
FspBI CTAG 2 cut(s) 104, 674
GluI GCNGC 3 cut(s) 276, 621, 733
HaeIII GGCC 1 cut(s) 212
Hin1II CATG 5 cut(s) 211, 267, 312, 579, 643
HinfI GANTC 2 cut(s) 319, 528
HphI GGTGA 2 cut(s) 580, 645
Hpy166II GTNNAC 1 cut(s) 29
Hpy188I TCNGA 1 cut(s) 516
Hpy188III TCNNGA 4 cut(s) 17, 328, 410, 674
Hpy8I GTNNAC 1 cut(s) 29
HpyAV CCTTC 2 cut(s) 367, 373
HpyCH4III ACNGT 2 cut(s) 143, 187
HpyCH4IV ACGT 1 cut(s) 507
HpyCH4V TGCA 4 cut(s) 222, 337, 347, 475
HpyF10VI GCNNNNNNNGC 1 cut(s) 532
HpyF3I CTNAG 3 cut(s) 76, 86, 424
HpySE526I ACGT 1 cut(s) 507
Hsp92II CATG 5 cut(s) 211, 267, 312, 579, 643
Kzo9I GATC 1 cut(s) 670
LmnI GCTCC 1 cut(s) 283
LpnPI CCDG 8 cut(s) 227, 298, 301, 308, 572, 653, 735, 740
Lsp1109I GCAGC 2 cut(s) 607, 719
MaeI CTAG 2 cut(s) 104, 674
MaeII ACGT 1 cut(s) 507
MaeIII GTNAC 4 cut(s) 41, 371, 642, 764
MalI GATC 1 cut(s) 672
MboI GATC 1 cut(s) 670
MboII GAAGA 2 cut(s) 32, 444
MflI RGATCY 1 cut(s) 670
MhlI GDGCHC 1 cut(s) 288
MluCI AATT 8 cut(s) 71, 136, 148, 217, 331, 340, 467, 694
MmeI TCCRAC 1 cut(s) 125
MnlI CCTC 7 cut(s) 102, 193, 265, 295, 370, 480, 779
MroXI GAANNNNTTC 1 cut(s) 12
MseI TTAA 4 cut(s) 81, 470, 554, 602
MslI CAYNNNNRTG 1 cut(s) 638
MwoI GCNNNNNNNGC 1 cut(s) 532
NdeII GATC 1 cut(s) 670
NlaIII CATG 5 cut(s) 211, 267, 312, 579, 643
NlaIV GGNNCC 4 cut(s) 261, 285, 306, 327
NmuCI GTSAC 1 cut(s) 642
NspI RCATGY 1 cut(s) 643
NspV TTCGAA 1 cut(s) 698
PdmI GAANNNNTTC 1 cut(s) 12
PfeI GAWTC 2 cut(s) 319, 528
PkrI GCNGC 3 cut(s) 277, 622, 734
PpuMI RGGWCCY 2 cut(s) 304, 325
PshBI ATTAAT 1 cut(s) 470
Psp5II RGGWCCY 2 cut(s) 304, 325
PspN4I GGNNCC 4 cut(s) 261, 285, 306, 327
PspPI GGNCC 4 cut(s) 211, 259, 304, 325
PspPPI RGGWCCY 2 cut(s) 304, 325
PsuI RGATCY 1 cut(s) 670
RseI CAYNNNNRTG 1 cut(s) 638
SaqAI TTAA 4 cut(s) 81, 470, 554, 602
SatI GCNGC 3 cut(s) 276, 621, 733
Sau3AI GATC 1 cut(s) 670
Sau96I GGNCC 4 cut(s) 211, 259, 304, 325
SduI GDGCHC 1 cut(s) 288
SfuI TTCGAA 1 cut(s) 698
SinI GGWCC 3 cut(s) 259, 304, 325
SmiMI CAYNNNNRTG 1 cut(s) 638
Sse9I AATT 8 cut(s) 71, 136, 148, 217, 331, 340, 467, 694
SsiI CCGC 2 cut(s) 233, 275
SspMI CTAG 2 cut(s) 104, 674
StyI CCWWGG 1 cut(s) 564
TaaI ACNGT 2 cut(s) 143, 187
TaiI ACGT 1 cut(s) 510
TaqI TCGA 2 cut(s) 134, 698
TasI AATT 8 cut(s) 71, 136, 148, 217, 331, 340, 467, 694
TauI GCSGC 1 cut(s) 278
TfiI GAWTC 2 cut(s) 319, 528
Tru1I TTAA 4 cut(s) 81, 470, 554, 602
Tru9I TTAA 4 cut(s) 81, 470, 554, 602
TscAI CASTG 1 cut(s) 591
TseFI GTSAC 1 cut(s) 642
TseI GCWGC 2 cut(s) 620, 732
Tsp45I GTSAC 1 cut(s) 642
TspDTI ATGAA 2 cut(s) 17, 196
TspGWI ACGGA 2 cut(s) 48, 89
TspRI CASTG 1 cut(s) 591
VpaK11BI GGWCC 3 cut(s) 259, 304, 325
VspI ATTAAT 1 cut(s) 470
XapI RAATTY 3 cut(s) 148, 331, 694
XbaI TCTAGA 1 cut(s) 673
XceI RCATGY 1 cut(s) 643
XmnI GAANNNNTTC 1 cut(s) 12
XspI CTAG 2 cut(s) 104, 674
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.