pycom03g20800

Serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
N/A
Physical Location & Seq
Forward (+)
21229195 .. 21230672
1478 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 927 bp
ATGTCTTTGTTTTTATCAATATTCTTCTCATGGCCTGCTGCTATTTTTGATATTATAGTCGAGATTTTGGATTGGGGGGAGGATGGTATTGCCACAACAGCTCAAACTGCTGCTTTTCGGGCGTCGTTTCAGCAAGAGGTTGCTGTTTGGCATAAGCTTGACCACCCAAATATTCCAAAGGTAAAACTAATATACGTTGATTCTTCGTATCATTCAATGGGAACTTCCAATCTTAAAATCCCTGTTAAAAACACATCAAACGATATTCACAATCGCCCTCCTTCCAGAGCTTGTTGCGTTGTTGTTGAGTATGTTCCTGGTGGGACGCTAAAGAACTTTTTGATTAGGAATAGAAGAAAGAAACTTGCCCTTAAGGTTGTGATTCAGCTTGCTTTAGTTCTTTCCAAAGGTTTGAGCTATCTTCACTCGAAGAAAATTGTACACCGTGAATTGAAAGCAGAAAATATGTTGCTAGATACTCGTAGAACATTGAAAGTTGCTGATTTTGTCGTTGCTCGAGTAGAAGCTCAGAACCCGAGGGACATGACTGGGGAGACTGGTACCCTTGCGTACATGGCACCAGAGGTCCTTGATGGCAAGCCATACAACAGGAAATGCGACATCTATAGTTTTGCTATTTGCTTATGGGAAATCTATTGTTGTGACATGCCTTATCCTAATCTAAGTTTTGCCGATGTCTCATCTGCAGTAGTGCGACAGTATTTACGACCAGAGATTCCACGATGTTGCTCGAGCACATTGGCCAGTGTCATGCGGAAATGTTGGGATGCAAGCCCGGATAAGCGCCCTGAAATGGAACCGGTAGTGAAGCTGTTGGATGCAATAGATACGAGCAAGGGAGGCGGCATGATACCTGAAGACCAGTCCACCGGCTGTTTCTGTTTCGGCACACCTCGCGGTCCATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Pkinase_Tyr

Protein Analysis

309

Amino Acids

34.53

Weight (kDa)

8.69

Isoelectric Point (pI)

37.84

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Pkinase PF00069 40 - 276 1.1e-38 Protein kinase domain
PK_Tyr_Ser-Thr PF07714 96 - 278 2.1e-46 Protein tyrosine and serine/threonine kinase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 560
AccB1I GGYRCC 2 cut(s) 560, 577
AccII CGCG 1 cut(s) 918
AciI CCGC 3 cut(s) 775, 864, 918
AcoI YGGCCR 1 cut(s) 762
AcuI CTGAAG 1 cut(s) 897
AcyI GRCGYC 1 cut(s) 122
AfaI GTAC 3 cut(s) 441, 562, 572
AfiI CCNNNNNNNGG 1 cut(s) 814
AflII CTTAAG 1 cut(s) 371
AgeI ACCGGT 1 cut(s) 820
AgsI TTSAA 3 cut(s) 216, 454, 493
AjnI CCWGG 1 cut(s) 316
AluBI AGCT 7 cut(s) 101, 157, 290, 388, 417, 527, 832
AluI AGCT 7 cut(s) 101, 157, 290, 388, 417, 527, 832
Alw21I GWGCWC 1 cut(s) 758
Alw26I GTCTC 2 cut(s) 548, 703
Ama87I CYCGRG 3 cut(s) 516, 535, 751
AoxI GGCC 2 cut(s) 32, 762
ApeKI GCWGC 2 cut(s) 38, 110
AsiGI ACCGGT 1 cut(s) 820
Asp718I GGTACC 1 cut(s) 560
AspLEI GCGC 1 cut(s) 807
AspS9I GGNCC 2 cut(s) 586, 920
AsuC2I CCSGG 1 cut(s) 797
AvaI CYCGRG 3 cut(s) 516, 535, 751
AvaII GGWCC 2 cut(s) 586, 920
BalI TGGCCA 1 cut(s) 764
BanI GGYRCC 2 cut(s) 560, 577
BbsI GAAGAC 1 cut(s) 885
Bbv12I GWGCWC 1 cut(s) 758
BbvI GCAGC 2 cut(s) 25, 97
BccI CCATC 2 cut(s) 77, 587
BciT130I CCWGG 1 cut(s) 318
BcnI CCSGG 1 cut(s) 797
BcoDI GTCTC 2 cut(s) 548, 703
BfaI CTAG 1 cut(s) 473
BfmI CTRYAG 2 cut(s) 625, 705
BfoI RGCGCY 1 cut(s) 808
BfrI CTTAAG 1 cut(s) 371
BisI GCNGC 3 cut(s) 39, 111, 865
BlsI GCNGC 3 cut(s) 40, 112, 866
Bme1390I CCNGG 2 cut(s) 318, 797
Bme18I GGWCC 2 cut(s) 586, 920
BmeT110I CYCGRG 3 cut(s) 516, 535, 751
BmgT120I GGNCC 2 cut(s) 586, 920
BmiI GGNNCC 3 cut(s) 562, 579, 819
BmrFI CCNGG 2 cut(s) 318, 797
BmrI ACTGGG 1 cut(s) 558
BmsI GCATC 2 cut(s) 778, 829
BmuI ACTGGG 1 cut(s) 558
BpiI GAAGAC 1 cut(s) 885
BpuMI CCSGG 1 cut(s) 797
BsaHI GRCGYC 1 cut(s) 122
BsaJI CCNNGG 1 cut(s) 536
BsaWI WCCGGW 1 cut(s) 820
Bsc4I CCNNNNNNNGG 1 cut(s) 814
Bse118I RCCGGY 2 cut(s) 820, 890
Bse1I ACTGG 4 cut(s) 553, 562, 765, 883
BseBI CCWGG 1 cut(s) 318
BseDI CCNNGG 1 cut(s) 536
BseGI GGATG 3 cut(s) 88, 793, 844
BseLI CCNNNNNNNGG 1 cut(s) 814
BseMII CTCAG 1 cut(s) 542
BseNI ACTGG 4 cut(s) 553, 562, 765, 883
BseXI GCAGC 2 cut(s) 25, 97
Bsh1236I CGCG 1 cut(s) 918
BshFI GGCC 2 cut(s) 34, 764
BshNI GGYRCC 2 cut(s) 560, 577
BshTI ACCGGT 1 cut(s) 820
BsiHKAI GWGCWC 1 cut(s) 758
BsiHKCI CYCGRG 3 cut(s) 516, 535, 751
BsiSI CCGG 3 cut(s) 797, 821, 891
BslFI GGGAC 2 cut(s) 337, 554
BslI CCNNNNNNNGG 1 cut(s) 814
BsmAI GTCTC 2 cut(s) 548, 703
BsmFI GGGAC 2 cut(s) 337, 554
BsnI GGCC 2 cut(s) 34, 764
BsoBI CYCGRG 3 cut(s) 516, 535, 751
Bsp1286I GDGCHC 1 cut(s) 758
Bsp1407I TGTACA 1 cut(s) 439
BspACI CCGC 3 cut(s) 775, 864, 918
BspANI GGCC 2 cut(s) 34, 764
BspCNI CTCAG 1 cut(s) 541
BspFNI CGCG 1 cut(s) 918
BspLI GGNNCC 3 cut(s) 562, 579, 819
BspMAI CTGCAG 1 cut(s) 709
BspT107I GGYRCC 2 cut(s) 560, 577
BspTI CTTAAG 1 cut(s) 371
BsrFI RCCGGY 2 cut(s) 820, 890
BsrGI TGTACA 1 cut(s) 439
BsrI ACTGG 4 cut(s) 553, 562, 765, 883
BssAI RCCGGY 2 cut(s) 820, 890
BssECI CCNNGG 1 cut(s) 536
BssNI GRCGYC 1 cut(s) 122
Bst2UI CCWGG 1 cut(s) 318
Bst4CI ACNGT 2 cut(s) 446, 720
BstACI GRCGYC 1 cut(s) 122
BstAFI CTTAAG 1 cut(s) 371
BstAUI TGTACA 1 cut(s) 439
BstC8I GCNNGC 4 cut(s) 36, 390, 599, 793
BstDEI CTNAG 2 cut(s) 528, 683
BstF5I GGATG 3 cut(s) 88, 793, 844
BstFNI CGCG 1 cut(s) 918
BstH2I RGCGCY 1 cut(s) 808
BstHHI GCGC 1 cut(s) 807
BstMAI GTCTC 2 cut(s) 548, 703
BstMWI GCNNNNNNNGC 6 cut(s) 98, 107, 119, 575, 861, 915
BstNI CCWGG 1 cut(s) 318
BstNSI RCATGY 1 cut(s) 670
BstSCI CCNGG 2 cut(s) 316, 795
BstSFI CTRYAG 2 cut(s) 625, 705
BstUI CGCG 1 cut(s) 918
BstV1I GCAGC 2 cut(s) 25, 97
BstV2I GAAGAC 1 cut(s) 885
BsuRI GGCC 2 cut(s) 34, 764
BtsCI GGATG 3 cut(s) 88, 793, 844
BtsIMutI CAGTG 1 cut(s) 772
Cac8I GCNNGC 4 cut(s) 36, 390, 599, 793
CfoI GCGC 1 cut(s) 807
Cfr10I RCCGGY 2 cut(s) 820, 890
Cfr13I GGNCC 2 cut(s) 586, 920
CseI GACGC 2 cut(s) 111, 334
Csp6I GTAC 3 cut(s) 440, 561, 571
CspAI ACCGGT 1 cut(s) 820
CviAII CATG 7 cut(s) 30, 544, 574, 667, 772, 868, 924
CviQI GTAC 3 cut(s) 440, 561, 571
DdeI CTNAG 2 cut(s) 528, 683
EaeI YGGCCR 1 cut(s) 762
Eco47I GGWCC 2 cut(s) 586, 920
Eco57I CTGAAG 1 cut(s) 897
Eco88I CYCGRG 3 cut(s) 516, 535, 751
EcoO109I RGGNCCY 1 cut(s) 586
EcoRII CCWGG 1 cut(s) 316
FaeI CATG 7 cut(s) 33, 547, 577, 670, 775, 871, 927
FaqI GGGAC 2 cut(s) 337, 554
FatI CATG 7 cut(s) 29, 543, 573, 666, 771, 867, 923
Fnu4HI GCNGC 3 cut(s) 39, 111, 865
FokI GGATG 3 cut(s) 95, 800, 851
Fsp4HI GCNGC 3 cut(s) 39, 111, 865
FspBI CTAG 1 cut(s) 473
GlaI GCGC 1 cut(s) 806
GluI GCNGC 3 cut(s) 39, 111, 865
HaeII RGCGCY 1 cut(s) 808
HaeIII GGCC 2 cut(s) 34, 764
HapII CCGG 3 cut(s) 797, 821, 891
HgaI GACGC 2 cut(s) 111, 334
HhaI GCGC 1 cut(s) 807
Hin1I GRCGYC 1 cut(s) 122
Hin1II CATG 7 cut(s) 33, 547, 577, 670, 775, 871, 927
Hin6I GCGC 1 cut(s) 805
HinP1I GCGC 1 cut(s) 805
HindIII AAGCTT 1 cut(s) 155
HinfI GANTC 3 cut(s) 200, 382, 736
HpaII CCGG 3 cut(s) 797, 821, 891
Hpy166II GTNNAC 2 cut(s) 442, 888
Hpy188I TCNGA 1 cut(s) 531
Hpy188III TCNNGA 2 cut(s) 61, 285
Hpy8I GTNNAC 2 cut(s) 442, 888
Hpy99I CGWCG 1 cut(s) 127
HpyAV CCTTC 1 cut(s) 291
HpyCH4III ACNGT 2 cut(s) 446, 720
HpyCH4IV ACGT 1 cut(s) 195
HpyCH4V TGCA 3 cut(s) 707, 791, 842
HpyF10VI GCNNNNNNNGC 6 cut(s) 98, 107, 119, 575, 861, 915
HpyF3I CTNAG 2 cut(s) 528, 683
HpySE526I ACGT 1 cut(s) 195
Hsp92I GRCGYC 1 cut(s) 122
Hsp92II CATG 7 cut(s) 33, 547, 577, 670, 775, 871, 927
HspAI GCGC 1 cut(s) 805
KpnI GGTACC 1 cut(s) 564
Lsp1109I GCAGC 2 cut(s) 25, 97
LweI GCATC 2 cut(s) 778, 829
MaeI CTAG 1 cut(s) 473
MaeII ACGT 1 cut(s) 195
MaeIII GTNAC 1 cut(s) 662
MboII GAAGA 6 cut(s) 16, 195, 366, 413, 442, 890
MhlI GDGCHC 1 cut(s) 758
MlsI TGGCCA 1 cut(s) 764
MluCI AATT 2 cut(s) 435, 449
MluNI TGGCCA 1 cut(s) 764
MmeI TCCRAC 1 cut(s) 816
MnlI CCTC 7 cut(s) 73, 130, 288, 531, 577, 854, 924
Mox20I TGGCCA 1 cut(s) 764
MscI TGGCCA 1 cut(s) 764
MseI TTAA 3 cut(s) 234, 246, 372
Msp20I TGGCCA 1 cut(s) 764
MspCI CTTAAG 1 cut(s) 371
MspI CCGG 3 cut(s) 797, 821, 891
MspR9I CCNGG 2 cut(s) 318, 797
MvaI CCWGG 1 cut(s) 318
MvnI CGCG 1 cut(s) 918
MwoI GCNNNNNNNGC 6 cut(s) 98, 107, 119, 575, 861, 915
NciI CCSGG 1 cut(s) 797
NlaIII CATG 7 cut(s) 33, 547, 577, 670, 775, 871, 927
NlaIV GGNNCC 3 cut(s) 562, 579, 819
NmuCI GTSAC 1 cut(s) 662
NspI RCATGY 1 cut(s) 670
PaeR7I CTCGAG 2 cut(s) 516, 751
PfeI GAWTC 3 cut(s) 200, 382, 736
PinAI ACCGGT 1 cut(s) 820
PkrI GCNGC 3 cut(s) 40, 112, 866
PpuMI RGGWCCY 1 cut(s) 586
Psp5II RGGWCCY 1 cut(s) 586
Psp6I CCWGG 1 cut(s) 316
PspGI CCWGG 1 cut(s) 316
PspN4I GGNNCC 3 cut(s) 562, 579, 819
PspPI GGNCC 2 cut(s) 586, 920
PspPPI RGGWCCY 1 cut(s) 586
PspXI VCTCGAGB 2 cut(s) 516, 751
PstI CTGCAG 1 cut(s) 709
RsaI GTAC 3 cut(s) 441, 562, 572
RsaNI GTAC 3 cut(s) 440, 561, 571
SaqAI TTAA 3 cut(s) 234, 246, 372
SatI GCNGC 3 cut(s) 39, 111, 865
Sau96I GGNCC 2 cut(s) 586, 920
ScrFI CCNGG 2 cut(s) 318, 797
SduI GDGCHC 1 cut(s) 758
SfaNI GCATC 2 cut(s) 778, 829
SfcI CTRYAG 2 cut(s) 625, 705
Sfr274I CTCGAG 2 cut(s) 516, 751
SinI GGWCC 2 cut(s) 586, 920
SlaI CTCGAG 2 cut(s) 516, 751
SmlI CTYRAG 3 cut(s) 371, 516, 751
SmoI CTYRAG 3 cut(s) 371, 516, 751
Sse9I AATT 2 cut(s) 435, 449
SsiI CCGC 3 cut(s) 775, 864, 918
SspI AATATT 2 cut(s) 21, 172
SspMI CTAG 1 cut(s) 473
StyD4I CCNGG 2 cut(s) 316, 795
TaaI ACNGT 2 cut(s) 446, 720
TaiI ACGT 1 cut(s) 198
TaqI TCGA 4 cut(s) 60, 428, 517, 752
TasI AATT 2 cut(s) 435, 449
TatI WGTACW 1 cut(s) 439
TauI GCSGC 1 cut(s) 867
TfiI GAWTC 3 cut(s) 200, 382, 736
Tru1I TTAA 3 cut(s) 234, 246, 372
Tru9I TTAA 3 cut(s) 234, 246, 372
TscAI CASTG 1 cut(s) 772
TseFI GTSAC 1 cut(s) 662
TseI GCWGC 2 cut(s) 38, 110
Tsp45I GTSAC 1 cut(s) 662
TspRI CASTG 1 cut(s) 772
Vha464I CTTAAG 1 cut(s) 371
VpaK11BI GGWCC 2 cut(s) 586, 920
XceI RCATGY 1 cut(s) 670
XhoI CTCGAG 2 cut(s) 516, 751
XspI CTAG 1 cut(s) 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.