pycom03g22850

60S ribosomal protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
N/A
Physical Location & Seq
Forward (+)
22895059 .. 22896429
1371 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 561 bp
ATGTTTGTAGTTAAGTCTTATACTCGGGCTTTGGGCCCGTACCCGAAAGATAAATTAAAAACCCGCGAATCCAAAACCCTAGCACCTTTTATTCTACTGAGGGCTAACCCTAACATACGCAGCTCCCACACGCAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAAGCAAAGATGAGTCGTGGGGCGGCAGCAGGGACGAAGGGAGGGAAGAAGAAGGGAGCCTCGTTCACGATAGACTGCGCGAAGCCGGTGGAGGATAAGATCATGGACATCGCCTCACTGGAGAAGTTCCTCCAGGAGCGCATCAAGGTCGGCGGTAAGGCCGGCGCTCTCGGCGACTCCGTCACCGTCTCCCGCGAGAAGAACAAGATCACCGTCACCTCCGACTCCAACTTTTCCAAAAGGTATCTTAAATACTTGACGAAGAAGCACTTGAAGAAGCACAACGTGAGGGATTGGCTTCGCGTGATTGCATCCAACAAGGACCGCAATGTTTATGAATTGAGATACTTCAACATTGCTGAGAACGAGGGTGAGGAGGAAGACTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko03010 map03010
Pfam Domains
Protein Families
Ribosomal_L22e

Protein Analysis

187

Amino Acids

21.39

Weight (kDa)

9.94

Isoelectric Point (pI)

36.6

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L22e PF01776 76 - 185 4.1e-46 Ribosomal L22e protein family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 4 cut(s) 66, 254, 369, 477
AciI CCGC 5 cut(s) 64, 197, 327, 367, 499
AdeI CACNNNGTG 1 cut(s) 460
AfaI GTAC 1 cut(s) 41
AgsI TTSAA 2 cut(s) 448, 526
AjnI CCWGG 1 cut(s) 306
AluBI AGCT 1 cut(s) 123
AluI AGCT 1 cut(s) 123
Alw26I GTCTC 1 cut(s) 367
Ama87I CYCGRG 1 cut(s) 24
AoxI GGCC 2 cut(s) 34, 333
ApaI GGGCCC 1 cut(s) 38
ApeKI GCWGC 2 cut(s) 120, 200
AspLEI GCGC 3 cut(s) 254, 315, 341
AspS9I GGNCC 3 cut(s) 34, 35, 496
AsuHPI GGTGA 4 cut(s) 349, 376, 382, 557
AvaI CYCGRG 1 cut(s) 24
AvaII GGWCC 1 cut(s) 496
BaeGI GKGCMC 1 cut(s) 38
BanII GRGCYC 1 cut(s) 38
BbvI GCAGC 2 cut(s) 132, 212
BcgI CGANNNNNNTGC 2 cut(s) 304, 338
BciT130I CCWGG 1 cut(s) 308
BcoDI GTCTC 1 cut(s) 367
BfaI CTAG 1 cut(s) 80
BfoI RGCGCY 1 cut(s) 342
BisI GCNGC 3 cut(s) 121, 198, 201
BlsI GCNGC 3 cut(s) 122, 199, 202
Bme1390I CCNGG 1 cut(s) 308
Bme18I GGWCC 1 cut(s) 496
BmeT110I CYCGRG 1 cut(s) 24
BmgT120I GGNCC 3 cut(s) 34, 35, 496
BmiI GGNNCC 2 cut(s) 36, 232
BmrFI CCNGG 1 cut(s) 308
BmsI GCATC 2 cut(s) 324, 494
BpmI CTGGAG 2 cut(s) 290, 314
Bse118I RCCGGY 2 cut(s) 259, 335
Bse1I ACTGG 1 cut(s) 297
Bse3DI GCAATG 2 cut(s) 508, 528
BseBI CCWGG 1 cut(s) 308
BseGI GGATG 1 cut(s) 485
BseMI GCAATG 2 cut(s) 508, 528
BseMII CTCAG 2 cut(s) 89, 525
BseNI ACTGG 1 cut(s) 297
BseSI GKGCMC 1 cut(s) 38
BseXI GCAGC 2 cut(s) 132, 212
Bsh1236I CGCG 4 cut(s) 66, 254, 369, 477
BshFI GGCC 2 cut(s) 36, 335
BsiHKCI CYCGRG 1 cut(s) 24
BsiSI CCGG 2 cut(s) 260, 336
BslFI GGGAC 1 cut(s) 220
BsmAI GTCTC 1 cut(s) 367
BsmBI CGTCTC 1 cut(s) 367
BsmFI GGGAC 1 cut(s) 220
BsnI GGCC 2 cut(s) 36, 335
BsoBI CYCGRG 1 cut(s) 24
Bsp120I GGGCCC 1 cut(s) 34
Bsp1286I GDGCHC 1 cut(s) 38
Bsp143I GATC 2 cut(s) 273, 381
BspACI CCGC 5 cut(s) 64, 197, 327, 367, 499
BspANI GGCC 2 cut(s) 36, 335
BspCNI CTCAG 2 cut(s) 90, 526
BspFNI CGCG 4 cut(s) 66, 254, 369, 477
BspLI GGNNCC 2 cut(s) 36, 232
BsrDI GCAATG 2 cut(s) 508, 528
BsrFI RCCGGY 2 cut(s) 259, 335
BsrI ACTGG 1 cut(s) 297
BssAI RCCGGY 2 cut(s) 259, 335
BssMI GATC 2 cut(s) 273, 381
Bst2UI CCWGG 1 cut(s) 308
Bst4CI ACNGT 2 cut(s) 361, 388
BstC8I GCNNGC 1 cut(s) 337
BstDEI CTNAG 2 cut(s) 98, 534
BstF5I GGATG 1 cut(s) 485
BstFNI CGCG 4 cut(s) 66, 254, 369, 477
BstH2I RGCGCY 1 cut(s) 342
BstHHI GCGC 3 cut(s) 254, 315, 341
BstKTI GATC 2 cut(s) 276, 384
BstMAI GTCTC 1 cut(s) 367
BstMBI GATC 2 cut(s) 273, 381
BstMWI GCNNNNNNNGC 1 cut(s) 345
BstNI CCWGG 1 cut(s) 308
BstSCI CCNGG 1 cut(s) 306
BstSLI GKGCMC 1 cut(s) 38
BstUI CGCG 4 cut(s) 66, 254, 369, 477
BstV1I GCAGC 2 cut(s) 132, 212
BsuRI GGCC 2 cut(s) 36, 335
BtgZI GCGATG 1 cut(s) 268
BtsCI GGATG 1 cut(s) 485
BtsIMutI CAGTG 1 cut(s) 290
Cac8I GCNNGC 1 cut(s) 337
CfoI GCGC 3 cut(s) 254, 315, 341
Cfr10I RCCGGY 2 cut(s) 259, 335
Cfr13I GGNCC 3 cut(s) 34, 35, 496
Csp6I GTAC 1 cut(s) 40
CviAII CATG 1 cut(s) 277
CviJI RGCY 8 cut(s) 29, 36, 104, 123, 233, 259, 335, 472
CviKI_1 RGCY 8 cut(s) 29, 36, 104, 123, 233, 259, 335, 472
CviQI GTAC 1 cut(s) 40
DdeI CTNAG 2 cut(s) 98, 534
DpnI GATC 2 cut(s) 275, 383
DpnII GATC 2 cut(s) 273, 381
DraIII CACNNNGTG 1 cut(s) 460
Eco24I GRGCYC 1 cut(s) 38
Eco47I GGWCC 1 cut(s) 496
Eco88I CYCGRG 1 cut(s) 24
EcoRII CCWGG 1 cut(s) 306
EcoT38I GRGCYC 1 cut(s) 38
Esp3I CGTCTC 1 cut(s) 367
FaeI CATG 1 cut(s) 280
FaiI YATR 4 cut(s) 21, 116, 278, 510
FalI AAGNNNNNCTT 2 cut(s) 428, 460
FaqI GGGAC 1 cut(s) 220
FatI CATG 1 cut(s) 276
FauI CCCGC 2 cut(s) 71, 374
Fnu4HI GCNGC 3 cut(s) 121, 198, 201
FokI GGATG 1 cut(s) 472
FriOI GRGCYC 1 cut(s) 38
Fsp4HI GCNGC 3 cut(s) 121, 198, 201
FspBI CTAG 1 cut(s) 80
GlaI GCGC 3 cut(s) 253, 314, 340
GluI GCNGC 3 cut(s) 121, 198, 201
GsuI CTGGAG 2 cut(s) 290, 314
HaeII RGCGCY 1 cut(s) 342
HaeIII GGCC 2 cut(s) 36, 335
HapII CCGG 2 cut(s) 260, 336
HhaI GCGC 3 cut(s) 254, 315, 341
Hin1II CATG 1 cut(s) 280
Hin6I GCGC 3 cut(s) 252, 313, 339
HinP1I GCGC 3 cut(s) 252, 313, 339
HinfI GANTC 4 cut(s) 68, 187, 350, 398
HpaII CCGG 2 cut(s) 260, 336
HphI GGTGA 4 cut(s) 349, 376, 382, 557
Hpy166II GTNNAC 1 cut(s) 240
Hpy188I TCNGA 1 cut(s) 397
Hpy188III TCNNGA 1 cut(s) 241
Hpy8I GTNNAC 1 cut(s) 240
HpyAV CCTTC 2 cut(s) 205, 220
HpyCH4III ACNGT 2 cut(s) 361, 388
HpyCH4IV ACGT 1 cut(s) 459
HpyCH4V TGCA 1 cut(s) 485
HpyF10VI GCNNNNNNNGC 1 cut(s) 345
HpyF3I CTNAG 2 cut(s) 98, 534
HpySE526I ACGT 1 cut(s) 459
Hsp92II CATG 1 cut(s) 280
HspAI GCGC 3 cut(s) 252, 313, 339
KroI GCCGGC 1 cut(s) 335
KroNI GCCGGC 1 cut(s) 337
Kzo9I GATC 2 cut(s) 273, 381
LmnI GCTCC 3 cut(s) 128, 230, 310
LpnPI CCDG 6 cut(s) 189, 273, 278, 293, 320, 349
Lsp1109I GCAGC 2 cut(s) 132, 212
LweI GCATC 2 cut(s) 324, 494
MaeI CTAG 1 cut(s) 80
MaeII ACGT 1 cut(s) 459
MaeIII GTNAC 2 cut(s) 355, 388
MalI GATC 2 cut(s) 275, 383
MboI GATC 2 cut(s) 273, 381
MboII GAAGA 5 cut(s) 232, 235, 385, 448, 460
MhlI GDGCHC 1 cut(s) 38
MluCI AATT 2 cut(s) 53, 512
MlyI GAGTC 3 cut(s) 196, 344, 392
MmeI TCCRAC 3 cut(s) 420, 426, 513
MroNI GCCGGC 1 cut(s) 335
MseI TTAA 3 cut(s) 12, 56, 423
MspI CCGG 2 cut(s) 260, 336
MspR9I CCNGG 1 cut(s) 308
MvaI CCWGG 1 cut(s) 308
MvnI CGCG 4 cut(s) 66, 254, 369, 477
MwoI GCNNNNNNNGC 1 cut(s) 345
NaeI GCCGGC 1 cut(s) 337
NdeII GATC 2 cut(s) 273, 381
NgoMIV GCCGGC 1 cut(s) 335
NlaIII CATG 1 cut(s) 280
NlaIV GGNNCC 2 cut(s) 36, 232
NmeAIII GCCGAG 1 cut(s) 324
NmuCI GTSAC 2 cut(s) 355, 388
PcsI WCGNNNNNNNCGW 1 cut(s) 351
PdiI GCCGGC 1 cut(s) 337
PfeI GAWTC 1 cut(s) 68
PflFI GACNNNGTC 1 cut(s) 353
PfoI TCCNGGA 1 cut(s) 306
PkrI GCNGC 3 cut(s) 122, 199, 202
PleI GAGTC 3 cut(s) 195, 344, 392
PpsI GAGTC 3 cut(s) 195, 344, 392
Psp6I CCWGG 1 cut(s) 306
PspGI CCWGG 1 cut(s) 306
PspN4I GGNNCC 2 cut(s) 36, 232
PspOMI GGGCCC 1 cut(s) 34
PspPI GGNCC 3 cut(s) 34, 35, 496
PsyI GACNNNGTC 1 cut(s) 353
RsaI GTAC 1 cut(s) 41
RsaNI GTAC 1 cut(s) 40
SaqAI TTAA 3 cut(s) 12, 56, 423
SatI GCNGC 3 cut(s) 121, 198, 201
Sau3AI GATC 2 cut(s) 273, 381
Sau96I GGNCC 3 cut(s) 34, 35, 496
SchI GAGTC 3 cut(s) 196, 344, 392
ScrFI CCNGG 1 cut(s) 308
SduI GDGCHC 1 cut(s) 38
SetI ASST 6 cut(s) 88, 125, 324, 395, 419, 462
SfaNI GCATC 2 cut(s) 324, 494
SinI GGWCC 1 cut(s) 496
Sse9I AATT 2 cut(s) 53, 512
SsiI CCGC 5 cut(s) 64, 197, 327, 367, 499
SspMI CTAG 1 cut(s) 80
StyD4I CCNGG 1 cut(s) 306
TaaI ACNGT 2 cut(s) 361, 388
TaiI ACGT 1 cut(s) 462
TasI AATT 2 cut(s) 53, 512
TauI GCSGC 1 cut(s) 200
TfiI GAWTC 1 cut(s) 68
Tru1I TTAA 3 cut(s) 12, 56, 423
Tru9I TTAA 3 cut(s) 12, 56, 423
TscAI CASTG 1 cut(s) 297
TseFI GTSAC 2 cut(s) 355, 388
TseI GCWGC 2 cut(s) 120, 200
Tsp45I GTSAC 2 cut(s) 355, 388
TspDTI ATGAA 1 cut(s) 525
TspGWI ACGGA 1 cut(s) 343
TspRI CASTG 1 cut(s) 297
Tth111I GACNNNGTC 1 cut(s) 353
VpaK11BI GGWCC 1 cut(s) 496
XspI CTAG 1 cut(s) 80
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.