pycom03g23440

helicase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
N/A
Physical Location & Seq
Forward (+)
23253115 .. 23253560
446 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 348 bp
ATGCCACTTTCCCCGACCCCCACCTCCCTCTCCTCCACTCTCTTCACCTCTTCCCCTCCCATCCAAAAACCCACCACATCCCCCCACCTCTGCCATCGTACTTGCCGGCTCTGCTGCCCTCACTTCCCGCCATCTTCTTCCTCAGTTCCAATCTCTGCAAACCCTAATTTCAATTCTCCACTTTCCCCCTATTTCTCCACTCAATCCCTAATTCGTTTCCCAAATGCAGCGCCTCTCGTTCTCCAAATCTATGAGATTCGAAAGAGGGTCGAGCTCAAAGCCGCTGTGTCCAAGCTCGAGCGACCGTGGGAGTTTGTCGAGAAAGCTCCGCAGGTTTGTTATACGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

116

Amino Acids

12.8

Weight (kDa)

9.36

Isoelectric Point (pI)

66.09

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 322
AciI CCGC 3 cut(s) 128, 282, 329
AfaI GTAC 1 cut(s) 100
AgsI TTSAA 1 cut(s) 172
AluBI AGCT 3 cut(s) 274, 295, 326
AluI AGCT 3 cut(s) 274, 295, 326
Alw21I GWGCWC 1 cut(s) 276
Ama87I CYCGRG 1 cut(s) 296
ApeKI GCWGC 2 cut(s) 114, 227
AspLEI GCGC 1 cut(s) 232
AsuHPI GGTGA 1 cut(s) 37
AsuII TTCGAA 1 cut(s) 259
AvaI CYCGRG 1 cut(s) 296
BanII GRGCYC 1 cut(s) 276
Bbv12I GWGCWC 1 cut(s) 276
BbvI GCAGC 2 cut(s) 101, 239
BccI CCATC 3 cut(s) 68, 102, 139
BfoI RGCGCY 1 cut(s) 233
BfuAI ACCTGC 1 cut(s) 322
BisI GCNGC 3 cut(s) 115, 228, 282
BlsI GCNGC 3 cut(s) 116, 229, 283
BmeT110I CYCGRG 1 cut(s) 296
Bpu14I TTCGAA 1 cut(s) 259
BsaAI YACGTR 1 cut(s) 345
BsaJI CCNNGG 1 cut(s) 305
Bse118I RCCGGY 1 cut(s) 105
BseDI CCNNGG 1 cut(s) 305
BseGI GGATG 2 cut(s) 60, 77
BseMII CTCAG 1 cut(s) 156
BseRI GAGGAG 1 cut(s) 22
BseXI GCAGC 2 cut(s) 101, 239
Bsh1285I CGRYCG 1 cut(s) 305
BsiEI CGRYCG 1 cut(s) 305
BsiHKAI GWGCWC 1 cut(s) 276
BsiHKCI CYCGRG 1 cut(s) 296
BsiSI CCGG 1 cut(s) 106
BsoBI CYCGRG 1 cut(s) 296
Bsp119I TTCGAA 1 cut(s) 259
Bsp1286I GDGCHC 1 cut(s) 276
BspACI CCGC 3 cut(s) 128, 282, 329
BspCNI CTCAG 1 cut(s) 155
BspMI ACCTGC 1 cut(s) 322
BspT104I TTCGAA 1 cut(s) 259
BsrFI RCCGGY 1 cut(s) 105
BssAI RCCGGY 1 cut(s) 105
BssECI CCNNGG 1 cut(s) 305
Bst4CI ACNGT 1 cut(s) 306
Bst6I CTCTTC 2 cut(s) 47, 55
BstBAI YACGTR 1 cut(s) 345
BstBI TTCGAA 1 cut(s) 259
BstC8I GCNNGC 1 cut(s) 107
BstDEI CTNAG 1 cut(s) 142
BstDSI CCRYGG 1 cut(s) 305
BstF5I GGATG 2 cut(s) 60, 77
BstH2I RGCGCY 1 cut(s) 233
BstHHI GCGC 1 cut(s) 232
BstMCI CGRYCG 1 cut(s) 305
BstMWI GCNNNNNNNGC 1 cut(s) 111
BstSNI TACGTA 1 cut(s) 345
BstV1I GCAGC 2 cut(s) 101, 239
BtgI CCRYGG 1 cut(s) 305
BtsCI GGATG 2 cut(s) 60, 77
BveI ACCTGC 1 cut(s) 322
Cac8I GCNNGC 1 cut(s) 107
CfoI GCGC 1 cut(s) 232
Cfr10I RCCGGY 1 cut(s) 105
Csp6I GTAC 1 cut(s) 99
CviJI RGCY 5 cut(s) 109, 274, 281, 295, 326
CviKI_1 RGCY 5 cut(s) 109, 274, 281, 295, 326
CviQI GTAC 1 cut(s) 99
DdeI CTNAG 1 cut(s) 142
Eam1104I CTCTTC 2 cut(s) 47, 55
EarI CTCTTC 2 cut(s) 47, 55
Ecl136II GAGCTC 1 cut(s) 274
Eco105I TACGTA 1 cut(s) 345
Eco24I GRGCYC 1 cut(s) 276
Eco53kI GAGCTC 1 cut(s) 274
Eco88I CYCGRG 1 cut(s) 296
EcoICRI GAGCTC 1 cut(s) 274
EcoT38I GRGCYC 1 cut(s) 276
FaiI YATR 2 cut(s) 252, 342
FauI CCCGC 1 cut(s) 135
Fnu4HI GCNGC 3 cut(s) 115, 228, 282
FokI GGATG 2 cut(s) 47, 64
FriOI GRGCYC 1 cut(s) 276
Fsp4HI GCNGC 3 cut(s) 115, 228, 282
GlaI GCGC 1 cut(s) 231
GluI GCNGC 3 cut(s) 115, 228, 282
HaeII RGCGCY 1 cut(s) 233
HapII CCGG 1 cut(s) 106
HhaI GCGC 1 cut(s) 232
Hin6I GCGC 1 cut(s) 230
HinP1I GCGC 1 cut(s) 230
HinfI GANTC 1 cut(s) 256
HpaII CCGG 1 cut(s) 106
HphI GGTGA 1 cut(s) 37
Hpy188III TCNNGA 1 cut(s) 319
HpyCH4III ACNGT 1 cut(s) 306
HpyCH4IV ACGT 1 cut(s) 344
HpyCH4V TGCA 2 cut(s) 158, 227
HpyF10VI GCNNNNNNNGC 1 cut(s) 111
HpyF3I CTNAG 1 cut(s) 142
HpySE526I ACGT 1 cut(s) 344
HspAI GCGC 1 cut(s) 230
KroI GCCGGC 1 cut(s) 105
KroNI GCCGGC 1 cut(s) 107
LmnI GCTCC 1 cut(s) 331
LpnPI CCDG 2 cut(s) 119, 317
Lsp1109I GCAGC 2 cut(s) 101, 239
MaeII ACGT 1 cut(s) 344
MboII GAAGA 4 cut(s) 34, 42, 126, 129
MhlI GDGCHC 1 cut(s) 276
MluCI AATT 3 cut(s) 166, 172, 210
MroNI GCCGGC 1 cut(s) 105
MspA1I CMGCKG 1 cut(s) 284
MspI CCGG 1 cut(s) 106
MwoI GCNNNNNNNGC 1 cut(s) 111
NaeI GCCGGC 1 cut(s) 107
NgoMIV GCCGGC 1 cut(s) 105
NspV TTCGAA 1 cut(s) 259
PaeR7I CTCGAG 1 cut(s) 296
PdiI GCCGGC 1 cut(s) 107
PfeI GAWTC 1 cut(s) 256
PkrI GCNGC 3 cut(s) 116, 229, 283
Ppu21I YACGTR 1 cut(s) 345
Psp124BI GAGCTC 1 cut(s) 276
PspXI VCTCGAGB 1 cut(s) 296
RsaI GTAC 1 cut(s) 100
RsaNI GTAC 1 cut(s) 99
SacI GAGCTC 1 cut(s) 276
SatI GCNGC 3 cut(s) 115, 228, 282
SduI GDGCHC 1 cut(s) 276
SetI ASST 8 cut(s) 26, 50, 90, 276, 297, 328, 336, 347
Sfr274I CTCGAG 1 cut(s) 296
SfuI TTCGAA 1 cut(s) 259
SlaI CTCGAG 1 cut(s) 296
SmlI CTYRAG 1 cut(s) 296
SmoI CTYRAG 1 cut(s) 296
SnaBI TACGTA 1 cut(s) 345
Sse9I AATT 3 cut(s) 166, 172, 210
SsiI CCGC 3 cut(s) 128, 282, 329
SstI GAGCTC 1 cut(s) 276
TaaI ACNGT 1 cut(s) 306
TaiI ACGT 1 cut(s) 347
TaqI TCGA 4 cut(s) 259, 270, 297, 318
TasI AATT 3 cut(s) 166, 172, 210
TauI GCSGC 1 cut(s) 284
TfiI GAWTC 1 cut(s) 256
TseI GCWGC 2 cut(s) 114, 227
XhoI CTCGAG 1 cut(s) 296
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.