pycom04g00290

Ankyrin repeat domain-containing protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0028687
Physical Location & Seq
Reverse (-)
285004 .. 286427
1424 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1257 bp
ATGCGGAGGATGAGGGATTTCTACATGGAAATTACATTCCACTTTGAGAGTTCTGTTGTACCTTTCATTTCGAGGATTGCCCCATCAGATACTTACAAGATCTGGAAAAAGGGTGCAAATTTGAGAGCAGATATGTCGTTGGCTGGTTATGATGGTTTGCGAATTAAGCGTCATGATAAGAGTGTTCTTTTCCTGGGTGATGGGTCTGAGGATGGTAAGGTGCCTCCCGGGTCACTTTGCATGGCCTCACACAAGGATAAGGAGGTGGTGAATGCTTTGGCCGGTTTTAGTTCTCAACCAACTGAAGAAAAGGTTCGGCAAAAGGTGGTTGCAATGTCTCGGACTGTGATGTCCAGGGGTAGGATTGATGTGACTCAGGCAGTACTTTTTCCTCAAATGACCTGGAAGCGGCAGAAGAAAACAGAAATGGTTGGCCCGTGGAAGGCTGTGGCATACGACATGCGTAATGTGGTTGTGAGTATGAAATCTAGAAGGGTTCCCGGGGCTATGACAGATGGCAAGCTCGTCTCGTCCTGCAATGAAAATGAAACAGAGAGCGAGGAGTTTAAGGATATTCTGACAGAGGATGAGATGAGGCAACTTGAAAATGCACTTTCGTCAGAATTATCAAACGAAAATGGTGATGGGAGTATTGAAGGTGGTTGTTATGAGCACAGGGAGATTCCTATTGAAGATGTTAGTAGTGATAGAAATGGAGAGAATAAGCAGGAAAAGAAGGGATGGTTCGGTGGACGGAGGAAGGGGGGTGATCACCAGAGAGGAAGGGATATGAAAGCATCTTCTTCCGCAAGTCCCGAAAATGAGAATCGTCACAAGGATGCTAGCAAGGAGAACGAGTGTAAGAAAGGATTAAGGCCAGTACTGTGGCTTTCCCCAAGCTTTCCATTACAAACAGAGGAACTGTTACCGTTACTTGATATTTTATCAAACAAGATCAAGGCAATTCGTCGTTTGAGGGAGCTGCTTACAACCAAACTTCCGAGGGGTACATTTCCAGTCAAGGTTGCTATCCCAGTGGTTCCTACTGTCAGACTAATTGTCACCTTCGTAAAGCTTGAAGAATTGCCTTCAACGGATGAGTTTGCAACACCGCCATCAAGCCCCACTGGCAGGGAGAGCCCAGGTTCGACAAGAGCTCCTTGCCAGCGCCCTATTCAAGACCCGTTTGCCATTCCGTCAGAATATAGATGGGTTACCAAATTAAAGAAAGGGAAGAGTCAGAGTCACCAAAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Ank_2 Ank_5 GPCR_chapero_1

Protein Analysis

419

Amino Acids

47.08

Weight (kDa)

9.34

Isoelectric Point (pI)

65.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANKRD13_C PF11904 42 - 403 3.8e-100 ANKRD13 C-terminal
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0028687)

Species Orthologous Gene IDs
malus_domestica MD04G1002800.v1.1
pyrus_communis pycom04g00290

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 349
AccB1I GGYRCC 1 cut(s) 220
AciI CCGC 4 cut(s) 4, 409, 807, 1112
AcoI YGGCCR 1 cut(s) 279
AcsI RAATTY 1 cut(s) 118
AcuI CTGAAG 1 cut(s) 324
AfaI GTAC 4 cut(s) 60, 384, 882, 1009
AfiI CCNNNNNNNGG 4 cut(s) 360, 408, 442, 1131
AgsI TTSAA 6 cut(s) 605, 656, 692, 1079, 1092, 1178
AhdI GACNNNNNGTC 1 cut(s) 1058
AjnI CCWGG 4 cut(s) 192, 353, 401, 1141
AluBI AGCT 5 cut(s) 523, 900, 982, 1075, 1157
AluI AGCT 5 cut(s) 523, 900, 982, 1075, 1157
Alw21I GWGCWC 2 cut(s) 675, 1159
Alw26I GTCTC 2 cut(s) 342, 532
Ama87I CYCGRG 2 cut(s) 227, 500
AoxI GGCC 4 cut(s) 243, 279, 433, 875
ApeKI GCWGC 1 cut(s) 982
ApoI RAATTY 1 cut(s) 118
ArsI GACNNNNNNTTYG 2 cut(s) 154, 186
Asp700I GAANNNNTTC 1 cut(s) 312
AspLEI GCGC 1 cut(s) 1170
AspS9I GGNCC 1 cut(s) 434
AsuC2I CCSGG 4 cut(s) 228, 229, 501, 502
AsuHPI GGTGA 7 cut(s) 209, 280, 653, 764, 779, 1054, 1238
AsuNHI GCTAGC 1 cut(s) 842
AvaI CYCGRG 2 cut(s) 227, 500
BanI GGYRCC 1 cut(s) 220
BanII GRGCYC 2 cut(s) 1142, 1159
Bbv12I GWGCWC 2 cut(s) 675, 1159
BbvI GCAGC 1 cut(s) 969
BccI CCATC 9 cut(s) 91, 146, 194, 206, 509, 638, 735, 1123, 1203
BcgI CGANNNNNNTGC 2 cut(s) 117, 151
BciT130I CCWGG 4 cut(s) 194, 355, 403, 1143
BclI TGATCA 1 cut(s) 769
BcnI CCSGG 4 cut(s) 228, 229, 501, 502
BcoDI GTCTC 2 cut(s) 342, 532
BfaI CTAG 2 cut(s) 489, 843
BfoI RGCGCY 1 cut(s) 1171
BglI GCCNNNNNGGC 1 cut(s) 1128
BglII AGATCT 1 cut(s) 99
BisI GCNGC 2 cut(s) 410, 983
BlsI GCNGC 2 cut(s) 411, 984
BmcAI AGTACT 2 cut(s) 384, 882
Bme1390I CCNGG 8 cut(s) 194, 228, 229, 355, 403, 501, 502, 1143
BmeRI GACNNNNNGTC 1 cut(s) 1058
BmeT110I CYCGRG 2 cut(s) 227, 500
BmgT120I GGNCC 1 cut(s) 434
BmiI GGNNCC 3 cut(s) 222, 498, 1041
BmrFI CCNGG 8 cut(s) 194, 228, 229, 355, 403, 501, 502, 1143
BmrI ACTGGG 1 cut(s) 1028
BmsI GCATC 2 cut(s) 806, 829
BmtI GCTAGC 1 cut(s) 846
BmuI ACTGGG 1 cut(s) 1028
BpuMI CCSGG 4 cut(s) 228, 229, 501, 502
BsaJI CCNNGG 8 cut(s) 193, 227, 354, 437, 500, 501, 1001, 1141
BsaXI ACNNNNNCTCC 4 cut(s) 842, 872, 1141, 1171
Bsc4I CCNNNNNNNGG 4 cut(s) 360, 408, 442, 1131
Bse118I RCCGGY 1 cut(s) 281
Bse1I ACTGG 4 cut(s) 878, 1016, 1034, 1132
Bse3DI GCAATG 2 cut(s) 339, 544
BseBI CCWGG 4 cut(s) 194, 355, 403, 1143
BseDI CCNNGG 8 cut(s) 193, 227, 354, 437, 500, 501, 1001, 1141
BseGI GGATG 6 cut(s) 15, 217, 592, 746, 844, 1102
BseLI CCNNNNNNNGG 4 cut(s) 360, 408, 442, 1131
BseMI GCAATG 2 cut(s) 339, 544
BseMII CTCAG 2 cut(s) 198, 389
BseNI ACTGG 4 cut(s) 878, 1016, 1034, 1132
BseRI GAGGAG 1 cut(s) 575
BseXI GCAGC 1 cut(s) 969
BshFI GGCC 4 cut(s) 245, 281, 435, 877
BshNI GGYRCC 1 cut(s) 220
BsiHKAI GWGCWC 2 cut(s) 675, 1159
BsiHKCI CYCGRG 2 cut(s) 227, 500
BsiSI CCGG 3 cut(s) 228, 282, 501
BslFI GGGAC 1 cut(s) 798
BslI CCNNNNNNNGG 4 cut(s) 360, 408, 442, 1131
BsmAI GTCTC 2 cut(s) 342, 532
BsmBI CGTCTC 1 cut(s) 532
BsmFI GGGAC 1 cut(s) 798
BsmI GAATGC 1 cut(s) 277
BsnI GGCC 4 cut(s) 245, 281, 435, 877
BsoBI CYCGRG 2 cut(s) 227, 500
Bsp1286I GDGCHC 3 cut(s) 675, 1142, 1159
Bsp143I GATC 3 cut(s) 99, 769, 954
BspACI CCGC 4 cut(s) 4, 409, 807, 1112
BspANI GGCC 4 cut(s) 245, 281, 435, 877
BspCNI CTCAG 2 cut(s) 199, 388
BspHI TCATGA 1 cut(s) 172
BspLI GGNNCC 3 cut(s) 222, 498, 1041
BspOI GCTAGC 1 cut(s) 846
BspT107I GGYRCC 1 cut(s) 220
BsrDI GCAATG 2 cut(s) 339, 544
BsrFI RCCGGY 1 cut(s) 281
BsrI ACTGG 4 cut(s) 878, 1016, 1034, 1132
BssAI RCCGGY 1 cut(s) 281
BssECI CCNNGG 8 cut(s) 193, 227, 354, 437, 500, 501, 1001, 1141
BssMI GATC 3 cut(s) 99, 769, 954
Bst2UI CCWGG 4 cut(s) 194, 355, 403, 1143
Bst4CI ACNGT 5 cut(s) 346, 885, 924, 930, 1048
Bst6I CTCTTC 1 cut(s) 1229
BstC8I GCNNGC 3 cut(s) 521, 844, 1166
BstDEI CTNAG 2 cut(s) 207, 375
BstDSI CCRYGG 1 cut(s) 437
BstEII GGTNACC 1 cut(s) 1213
BstF5I GGATG 6 cut(s) 15, 217, 592, 746, 844, 1102
BstH2I RGCGCY 1 cut(s) 1171
BstHHI GCGC 1 cut(s) 1170
BstKTI GATC 3 cut(s) 102, 772, 957
BstMAI GTCTC 2 cut(s) 342, 532
BstMBI GATC 3 cut(s) 99, 769, 954
BstMWI GCNNNNNNNGC 3 cut(s) 166, 1128, 1137
BstNI CCWGG 4 cut(s) 194, 355, 403, 1143
BstNSI RCATGY 1 cut(s) 463
BstPI GGTNACC 1 cut(s) 1213
BstSCI CCNGG 8 cut(s) 192, 226, 227, 353, 401, 499, 500, 1141
BstV1I GCAGC 1 cut(s) 969
BstX2I RGATCY 1 cut(s) 99
BstXI CCANNNNNNTGG 1 cut(s) 885
BstYI RGATCY 1 cut(s) 99
BsuRI GGCC 4 cut(s) 245, 281, 435, 877
BtgI CCRYGG 1 cut(s) 437
BtsCI GGATG 6 cut(s) 15, 217, 592, 746, 844, 1102
BtsIMutI CAGTG 2 cut(s) 1041, 1125
Cac8I GCNNGC 3 cut(s) 521, 844, 1166
CciI TCATGA 1 cut(s) 172
CfoI GCGC 1 cut(s) 1170
Cfr10I RCCGGY 1 cut(s) 281
Cfr13I GGNCC 1 cut(s) 434
Cfr9I CCCGGG 2 cut(s) 227, 500
CseI GACGC 1 cut(s) 158
Csp6I GTAC 4 cut(s) 59, 383, 881, 1008
CviAII CATG 4 cut(s) 25, 173, 241, 460
CviQI GTAC 4 cut(s) 59, 383, 881, 1008
DdeI CTNAG 2 cut(s) 207, 375
DpnI GATC 3 cut(s) 101, 771, 956
DpnII GATC 3 cut(s) 99, 769, 954
DrdI GACNNNNNNGTC 1 cut(s) 349
DriI GACNNNNNGTC 1 cut(s) 1058
DseDI GACNNNNNNGTC 1 cut(s) 349
EaeI YGGCCR 1 cut(s) 279
Eam1104I CTCTTC 1 cut(s) 1229
Eam1105I GACNNNNNGTC 1 cut(s) 1058
EarI CTCTTC 1 cut(s) 1229
Ecl136II GAGCTC 1 cut(s) 1157
Eco24I GRGCYC 2 cut(s) 1142, 1159
Eco53kI GAGCTC 1 cut(s) 1157
Eco57I CTGAAG 1 cut(s) 324
Eco88I CYCGRG 2 cut(s) 227, 500
Eco91I GGTNACC 1 cut(s) 1213
EcoICRI GAGCTC 1 cut(s) 1157
EcoO65I GGTNACC 1 cut(s) 1213
EcoRII CCWGG 4 cut(s) 192, 353, 401, 1141
EcoT38I GRGCYC 2 cut(s) 1142, 1159
Esp3I CGTCTC 1 cut(s) 532
FaeI CATG 4 cut(s) 28, 176, 244, 463
FalI AAGNNNNNCTT 2 cut(s) 1144, 1176
FaqI GGGAC 1 cut(s) 798
FatI CATG 4 cut(s) 24, 172, 240, 459
FbaI TGATCA 1 cut(s) 769
Fnu4HI GCNGC 2 cut(s) 410, 983
FokI GGATG 6 cut(s) 22, 224, 599, 753, 851, 1109
FriOI GRGCYC 2 cut(s) 1142, 1159
Fsp4HI GCNGC 2 cut(s) 410, 983
FspBI CTAG 2 cut(s) 489, 843
GlaI GCGC 1 cut(s) 1169
GluI GCNGC 2 cut(s) 410, 983
HaeII RGCGCY 1 cut(s) 1171
HaeIII GGCC 4 cut(s) 245, 281, 435, 877
HapII CCGG 3 cut(s) 228, 282, 501
HgaI GACGC 1 cut(s) 158
HhaI GCGC 1 cut(s) 1170
Hin1II CATG 4 cut(s) 28, 176, 244, 463
Hin6I GCGC 1 cut(s) 1168
HinP1I GCGC 1 cut(s) 1168
HindIII AAGCTT 2 cut(s) 898, 1073
HinfI GANTC 5 cut(s) 373, 682, 826, 1237, 1243
HpaII CCGG 3 cut(s) 228, 282, 501
HphI GGTGA 7 cut(s) 209, 280, 653, 764, 779, 1054, 1238
Hpy166II GTNNAC 1 cut(s) 752
Hpy188I TCNGA 9 cut(s) 88, 208, 342, 579, 622, 1002, 1052, 1201, 1242
Hpy188III TCNNGA 5 cut(s) 103, 173, 489, 815, 1178
Hpy8I GTNNAC 1 cut(s) 752
Hpy99I CGWCG 1 cut(s) 972
HpyAV CCTTC 8 cut(s) 436, 486, 650, 730, 754, 777, 1075, 1098
HpyCH4III ACNGT 5 cut(s) 346, 885, 924, 930, 1048
HpyCH4V TGCA 6 cut(s) 116, 240, 332, 537, 611, 1106
HpyF10VI GCNNNNNNNGC 3 cut(s) 166, 1128, 1137
HpyF3I CTNAG 2 cut(s) 207, 375
Hsp92II CATG 4 cut(s) 28, 176, 244, 463
HspAI GCGC 1 cut(s) 1168
Ksp22I TGATCA 1 cut(s) 769
Kzo9I GATC 3 cut(s) 99, 769, 954
LmnI GCTCC 2 cut(s) 979, 1162
Lsp1109I GCAGC 1 cut(s) 969
LweI GCATC 2 cut(s) 806, 829
MaeI CTAG 2 cut(s) 489, 843
MaeIII GTNAC 8 cut(s) 231, 370, 830, 924, 930, 1060, 1213, 1244
MalI GATC 3 cut(s) 101, 771, 956
MboI GATC 3 cut(s) 99, 769, 954
MboII GAAGA 7 cut(s) 317, 427, 704, 792, 795, 1091, 1246
MflI RGATCY 1 cut(s) 99
MhlI GDGCHC 3 cut(s) 675, 1142, 1159
MluCI AATT 8 cut(s) 30, 118, 162, 623, 963, 1056, 1082, 1220
MlyI GAGTC 3 cut(s) 367, 1246, 1252
MroXI GAANNNNTTC 1 cut(s) 312
MseI TTAA 4 cut(s) 165, 567, 872, 1223
MslI CAYNNNNRTG 1 cut(s) 837
MspI CCGG 3 cut(s) 228, 282, 501
MspR9I CCNGG 8 cut(s) 194, 228, 229, 355, 403, 501, 502, 1143
Mva1269I GAATGC 1 cut(s) 277
MvaI CCWGG 4 cut(s) 194, 355, 403, 1143
MwoI GCNNNNNNNGC 3 cut(s) 166, 1128, 1137
NciI CCSGG 4 cut(s) 228, 229, 501, 502
NdeII GATC 3 cut(s) 99, 769, 954
NheI GCTAGC 1 cut(s) 842
NlaIII CATG 4 cut(s) 28, 176, 244, 463
NlaIV GGNNCC 3 cut(s) 222, 498, 1041
NmuCI GTSAC 5 cut(s) 231, 370, 830, 1060, 1244
NspI RCATGY 1 cut(s) 463
PagI TCATGA 1 cut(s) 172
PctI GAATGC 1 cut(s) 277
PdmI GAANNNNTTC 1 cut(s) 312
PfeI GAWTC 2 cut(s) 682, 826
PkrI GCNGC 2 cut(s) 411, 984
PleI GAGTC 3 cut(s) 367, 1245, 1251
PpsI GAGTC 3 cut(s) 367, 1245, 1251
Psp124BI GAGCTC 1 cut(s) 1159
Psp6I CCWGG 4 cut(s) 192, 353, 401, 1141
PspEI GGTNACC 1 cut(s) 1213
PspGI CCWGG 4 cut(s) 192, 353, 401, 1141
PspN4I GGNNCC 3 cut(s) 222, 498, 1041
PspPI GGNCC 1 cut(s) 434
PsuI RGATCY 1 cut(s) 99
RsaI GTAC 4 cut(s) 60, 384, 882, 1009
RsaNI GTAC 4 cut(s) 59, 383, 881, 1008
RseI CAYNNNNRTG 1 cut(s) 837
SacI GAGCTC 1 cut(s) 1159
SaqAI TTAA 4 cut(s) 165, 567, 872, 1223
SatI GCNGC 2 cut(s) 410, 983
Sau3AI GATC 3 cut(s) 99, 769, 954
Sau96I GGNCC 1 cut(s) 434
ScaI AGTACT 2 cut(s) 384, 882
SchI GAGTC 3 cut(s) 367, 1246, 1252
ScrFI CCNGG 8 cut(s) 194, 228, 229, 355, 403, 501, 502, 1143
SduI GDGCHC 3 cut(s) 675, 1142, 1159
SfaNI GCATC 2 cut(s) 806, 829
SmaI CCCGGG 2 cut(s) 229, 502
SmiMI CAYNNNNRTG 1 cut(s) 837
Sse9I AATT 8 cut(s) 30, 118, 162, 623, 963, 1056, 1082, 1220
SsiI CCGC 4 cut(s) 4, 409, 807, 1112
SspMI CTAG 2 cut(s) 489, 843
SstI GAGCTC 1 cut(s) 1159
StyD4I CCNGG 8 cut(s) 192, 226, 227, 353, 401, 499, 500, 1141
TaaI ACNGT 5 cut(s) 346, 885, 924, 930, 1048
TaqI TCGA 2 cut(s) 71, 1148
TasI AATT 8 cut(s) 30, 118, 162, 623, 963, 1056, 1082, 1220
TatI WGTACW 2 cut(s) 382, 880
TauI GCSGC 1 cut(s) 412
TfiI GAWTC 2 cut(s) 682, 826
Tru1I TTAA 4 cut(s) 165, 567, 872, 1223
Tru9I TTAA 4 cut(s) 165, 567, 872, 1223
TscAI CASTG 2 cut(s) 1041, 1132
TseFI GTSAC 5 cut(s) 231, 370, 830, 1060, 1244
TseI GCWGC 1 cut(s) 982
Tsp45I GTSAC 5 cut(s) 231, 370, 830, 1060, 1244
TspDTI ATGAA 5 cut(s) 55, 497, 555, 561, 806
TspGWI ACGGA 3 cut(s) 769, 1109, 1185
TspMI CCCGGG 2 cut(s) 227, 500
TspRI CASTG 2 cut(s) 1041, 1132
XapI RAATTY 1 cut(s) 118
XbaI TCTAGA 1 cut(s) 488
XceI RCATGY 1 cut(s) 463
XmaI CCCGGG 2 cut(s) 227, 500
XmnI GAANNNNTTC 1 cut(s) 312
XspI CTAG 2 cut(s) 489, 843
ZrmI AGTACT 2 cut(s) 384, 882
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.