pycom04g01210

Alpha-1,4-glucan-protein synthase (UDP-forming)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
N/A
Physical Location & Seq
Reverse (-)
1085315 .. 1085623
309 bp
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UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 309 bp
ATGTGGAGGCCTTTCTTCCAGCAGTACCACCTCATCATCATCCAGGATGGCGACCCGTTGAAGGTGATCAAGGTCCCAGAGGGCTTCGACTACGAGCTTTACAACAGCAACGACATCAATAGGATTCTAGGTCCTAAGGCGTCATGCATCTCCTTCAAGGACTTTGCTTGCAAATGCTTCAGCTACATGGTCTCCAAGAAGAAGTACATCTACACCATCGACGAAGATTGCTTTGTAAGTATCGCTTTTGGGTTTCGAAAACTTGGGATCTTAATTGGGGCTGTTTTGGATGTGGGATTTGATTTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00520 map00520
Pfam Domains
Protein Families
RGP

Protein Analysis

103

Amino Acids

11.83

Weight (kDa)

6.53

Isoelectric Point (pI)

30.23

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RGP PF03214 1 - 80 7.7e-34 Reversibly glycosylated polypeptide
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AclWI GGATC 1 cut(s) 275
AcuI CTGAAG 1 cut(s) 163
AcyI GRCGYC 1 cut(s) 140
AfaI GTAC 2 cut(s) 26, 206
AfiI CCNNNNNNNGG 1 cut(s) 61
AgsI TTSAA 2 cut(s) 61, 157
AjnI CCWGG 1 cut(s) 42
AluBI AGCT 2 cut(s) 97, 183
AluI AGCT 2 cut(s) 97, 183
Alw26I GTCTC 1 cut(s) 196
AlwI GGATC 1 cut(s) 275
AoxI GGCC 1 cut(s) 8
AspS9I GGNCC 2 cut(s) 73, 131
AsuHPI GGTGA 1 cut(s) 76
AsuII TTCGAA 1 cut(s) 256
AvaII GGWCC 2 cut(s) 73, 131
AxyI CCTNAGG 1 cut(s) 135
BarI GAAGNNNNNNTAC 2 cut(s) 194, 226
BccI CCATC 2 cut(s) 41, 224
BciT130I CCWGG 1 cut(s) 44
BclI TGATCA 1 cut(s) 66
BcoDI GTCTC 1 cut(s) 196
BfaI CTAG 1 cut(s) 128
Bme1390I CCNGG 1 cut(s) 44
Bme18I GGWCC 2 cut(s) 73, 131
BmgT120I GGNCC 2 cut(s) 73, 131
BmiI GGNNCC 1 cut(s) 75
BmrFI CCNGG 1 cut(s) 44
BmsI GCATC 1 cut(s) 156
Bpu14I TTCGAA 1 cut(s) 256
BsaHI GRCGYC 1 cut(s) 140
BsaI GGTCTC 1 cut(s) 196
BsaXI ACNNNNNCTCC 2 cut(s) 176, 206
Bsc4I CCNNNNNNNGG 1 cut(s) 61
Bse21I CCTNAGG 1 cut(s) 135
BseBI CCWGG 1 cut(s) 44
BseGI GGATG 3 cut(s) 39, 52, 295
BseLI CCNNNNNNNGG 1 cut(s) 61
BshFI GGCC 1 cut(s) 10
BslFI GGGAC 1 cut(s) 59
BslI CCNNNNNNNGG 1 cut(s) 61
BsmAI GTCTC 1 cut(s) 196
BsmFI GGGAC 1 cut(s) 59
BsnI GGCC 1 cut(s) 10
Bso31I GGTCTC 1 cut(s) 196
Bsp119I TTCGAA 1 cut(s) 256
Bsp143I GATC 2 cut(s) 66, 267
BspANI GGCC 1 cut(s) 10
BspLI GGNNCC 1 cut(s) 75
BspPI GGATC 1 cut(s) 275
BspT104I TTCGAA 1 cut(s) 256
BspTNI GGTCTC 1 cut(s) 196
BssMI GATC 2 cut(s) 66, 267
BssNI GRCGYC 1 cut(s) 140
Bst2UI CCWGG 1 cut(s) 44
BstACI GRCGYC 1 cut(s) 140
BstBI TTCGAA 1 cut(s) 256
BstC8I GCNNGC 1 cut(s) 169
BstDEI CTNAG 1 cut(s) 135
BstF5I GGATG 3 cut(s) 39, 52, 295
BstKTI GATC 2 cut(s) 69, 270
BstMAI GTCTC 1 cut(s) 196
BstMBI GATC 2 cut(s) 66, 267
BstNI CCWGG 1 cut(s) 44
BstSCI CCNGG 1 cut(s) 42
BstX2I RGATCY 1 cut(s) 267
BstYI RGATCY 1 cut(s) 267
Bsu36I CCTNAGG 1 cut(s) 135
BsuRI GGCC 1 cut(s) 10
BtsCI GGATG 3 cut(s) 39, 52, 295
Cac8I GCNNGC 1 cut(s) 169
Cfr13I GGNCC 2 cut(s) 73, 131
CseI GACGC 1 cut(s) 129
Csp6I GTAC 2 cut(s) 25, 205
CviAII CATG 2 cut(s) 144, 187
CviJI RGCY 5 cut(s) 10, 84, 97, 183, 281
CviKI_1 RGCY 5 cut(s) 10, 84, 97, 183, 281
CviQI GTAC 2 cut(s) 25, 205
DdeI CTNAG 1 cut(s) 135
DpnI GATC 2 cut(s) 68, 269
DpnII GATC 2 cut(s) 66, 267
Eco147I AGGCCT 1 cut(s) 10
Eco31I GGTCTC 1 cut(s) 196
Eco47I GGWCC 2 cut(s) 73, 131
Eco57I CTGAAG 1 cut(s) 163
Eco81I CCTNAGG 1 cut(s) 135
EcoO109I RGGNCCY 2 cut(s) 73, 131
EcoRII CCWGG 1 cut(s) 42
EcoT22I ATGCAT 1 cut(s) 149
FaeI CATG 2 cut(s) 147, 190
FaiI YATR 2 cut(s) 145, 188
FalI AAGNNNNNCTT 2 cut(s) 229, 261
FaqI GGGAC 1 cut(s) 59
FatI CATG 2 cut(s) 143, 186
FbaI TGATCA 1 cut(s) 66
FokI GGATG 3 cut(s) 26, 59, 302
FspBI CTAG 1 cut(s) 128
HaeIII GGCC 1 cut(s) 10
HgaI GACGC 1 cut(s) 129
Hin1I GRCGYC 1 cut(s) 140
Hin1II CATG 2 cut(s) 147, 190
HinfI GANTC 1 cut(s) 124
HphI GGTGA 1 cut(s) 76
Hpy99I CGWCG 1 cut(s) 224
HpyAV CCTTC 2 cut(s) 55, 163
HpyCH4V TGCA 2 cut(s) 147, 171
HpyF3I CTNAG 1 cut(s) 135
Hsp92I GRCGYC 1 cut(s) 140
Hsp92II CATG 2 cut(s) 147, 190
Ksp22I TGATCA 1 cut(s) 66
Kzo9I GATC 2 cut(s) 66, 267
LpnPI CCDG 4 cut(s) 29, 32, 56, 90
LweI GCATC 1 cut(s) 156
MaeI CTAG 1 cut(s) 128
MalI GATC 2 cut(s) 68, 269
MboI GATC 2 cut(s) 66, 267
MboII GAAGA 3 cut(s) 7, 211, 236
MflI RGATCY 1 cut(s) 267
MluCI AATT 1 cut(s) 273
MnlI CCTC 2 cut(s) 41, 73
Mph1103I ATGCAT 1 cut(s) 149
MseI TTAA 1 cut(s) 272
MspR9I CCNGG 1 cut(s) 44
MvaI CCWGG 1 cut(s) 44
NdeII GATC 2 cut(s) 66, 267
NlaIII CATG 2 cut(s) 147, 190
NlaIV GGNNCC 1 cut(s) 75
NsiI ATGCAT 1 cut(s) 149
NspV TTCGAA 1 cut(s) 256
PceI AGGCCT 1 cut(s) 10
PfeI GAWTC 1 cut(s) 124
PfoI TCCNGGA 1 cut(s) 42
PpuMI RGGWCCY 2 cut(s) 73, 131
Psp5II RGGWCCY 2 cut(s) 73, 131
Psp6I CCWGG 1 cut(s) 42
PspGI CCWGG 1 cut(s) 42
PspN4I GGNNCC 1 cut(s) 75
PspPI GGNCC 2 cut(s) 73, 131
PspPPI RGGWCCY 2 cut(s) 73, 131
PsuI RGATCY 1 cut(s) 267
RsaI GTAC 2 cut(s) 26, 206
RsaNI GTAC 2 cut(s) 25, 205
SaqAI TTAA 1 cut(s) 272
Sau3AI GATC 2 cut(s) 66, 267
Sau96I GGNCC 2 cut(s) 73, 131
ScrFI CCNGG 1 cut(s) 44
SetI ASST 6 cut(s) 33, 66, 75, 99, 133, 185
SfaNI GCATC 1 cut(s) 156
SfuI TTCGAA 1 cut(s) 256
SinI GGWCC 2 cut(s) 73, 131
Sse9I AATT 1 cut(s) 273
SseBI AGGCCT 1 cut(s) 10
SspMI CTAG 1 cut(s) 128
StuI AGGCCT 1 cut(s) 10
StyD4I CCNGG 1 cut(s) 42
TaqI TCGA 3 cut(s) 87, 219, 256
TasI AATT 1 cut(s) 273
TatI WGTACW 1 cut(s) 204
TfiI GAWTC 1 cut(s) 124
Tru1I TTAA 1 cut(s) 272
Tru9I TTAA 1 cut(s) 272
VpaK11BI GGWCC 2 cut(s) 73, 131
XspI CTAG 1 cut(s) 128
Zsp2I ATGCAT 1 cut(s) 149
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.