pycom04g01800

Cell division cycle

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
N/A
Physical Location & Seq
Reverse (-)
1654278 .. 1655122
845 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 801 bp
ATGATTTTGCAGGGTTCAGAGAAGACGCTGGATGCTCCCGGGATTGTAGATGATTTCTACTTGAATGTACTCGACTGGAGCAGCAATAATGTTCTTGCAATTGCTCTAGGAAACACGGTGTACCTATGGAGTGCTTCTGATGCATCTGTTGAAGAACTTGTCACAGTTGATGACGAAGCTGGCCCCATTACAAGCATCAGTTGGGCTCCTGATGGGCAGCATGTTGCTGTTGGCTTGAACAATTCCAATGTTCAATTGTGGGATTCCATCTCTGTTAAACTGGTAAGAACATTGTTATTGCTTATTCTACTGTTAAACAAAACTTTGTTGCTAAATTTACTACTCATTATGCAGTTGCGAGTGTTGAGAGATGGTCACCAACAAAGAGTGGGTTCACTGGCCTGGAATAAGCACATACTTACCACAGGTGGTGGGGATAGTAAAATAATCGACAATGACCTTAGAGTGAGATCTCATATAGTTGAAACCTACATCGGTCACCATCAGGAAGTTTGTGGATTGAAATGGTCAGCGTCGGGCCAACAATTAGCTAGTGGAGGGAACGACAATCTTCTCTTTATATGGGATAGGTCGGCAACCTCTTCAACTTCATCACACCAGTGGCTTCACAGGCTTCAAAACCATTCTGCAGCAGTCAAGGCCCTTGCTTGGTGTCCATTCCAAGGGAATTTGCTGGCCTCTGGTGGAGGTGAAGGTGACCAGTGCATTAAGTTCTGGAATGCACACACAGGTGCGTGCTTGAACTCGGTTGAAACTGGCTCGCAATTCTCACGGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04110 ko04111 ko04113 ko04114 ko04120 ko05166 ko05203 map04110 map04111 map04113 map04114 map04120 map05166 map05203
Pfam Domains
Protein Families
ANAPC4_WD40 WD40

Protein Analysis

267

Amino Acids

29.05

Weight (kDa)

5.74

Isoelectric Point (pI)

35.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 10 - 98 1.8e-29 CDC20/Fizzy WD40 domain
WD40_WDHD1_1st PF24817 21 - 95 2.7e-06 WDHD1 first WD40 domain
Beta-prop_THOC3 PF25174 30 - 97 1.1e-06 THOC3 beta-propeller domain
WD40_CDC20-Fz PF24807 124 - 264 5e-52 CDC20/Fizzy WD40 domain
Beta-prop_WDR5 PF25175 124 - 257 8.6e-15 WDR5 beta-propeller domain
WD40_Prp19 PF24814 136 - 260 6.8e-12 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 160 - 258 1e-14 WDR3 first beta-propeller domain
WD40 PF00400 161 - 196 6.6e-07 WD domain, G-beta repeat
Beta-prop_CAF1B_HIR1 PF24105 161 - 256 4.5e-07 CAF1B/HIR1 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 164 - 254 8e-06 TEP-1 second beta-propeller
Beta-prop_THOC3 PF25174 167 - 262 3.6e-18 THOC3 beta-propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 2 cut(s) 334, 688
AfaI GTAC 2 cut(s) 69, 122
AfiI CCNNNNNNNGG 2 cut(s) 669, 683
AjnI CCWGG 1 cut(s) 401
AleI CACNNNNGTG 2 cut(s) 619, 750
AluBI AGCT 2 cut(s) 179, 551
AluI AGCT 2 cut(s) 179, 551
Ama87I CYCGRG 1 cut(s) 38
AoxI GGCC 5 cut(s) 181, 399, 538, 660, 696
ApeKI GCWGC 3 cut(s) 81, 217, 650
ApoI RAATTY 2 cut(s) 334, 688
AspS9I GGNCC 3 cut(s) 182, 538, 661
AsuC2I CCSGG 2 cut(s) 39, 40
AsuHPI GGTGA 4 cut(s) 368, 491, 722, 728
AvaI CYCGRG 1 cut(s) 38
BanII GRGCYC 1 cut(s) 208
BbsI GAAGAC 1 cut(s) 29
BbvI GCAGC 3 cut(s) 93, 229, 662
BccI CCATC 4 cut(s) 206, 275, 365, 510
BciT130I CCWGG 1 cut(s) 403
BcnI CCSGG 2 cut(s) 39, 40
BfaI CTAG 2 cut(s) 107, 552
BfmI CTRYAG 1 cut(s) 648
BglII AGATCT 1 cut(s) 470
BisI GCNGC 3 cut(s) 82, 218, 651
BlsI GCNGC 3 cut(s) 83, 219, 652
Bme1390I CCNGG 3 cut(s) 39, 40, 403
BmeT110I CYCGRG 1 cut(s) 38
BmgT120I GGNCC 3 cut(s) 182, 538, 661
BmiI GGNNCC 2 cut(s) 184, 207
BmrFI CCNGG 3 cut(s) 39, 40, 403
BmsI GCATC 4 cut(s) 22, 130, 152, 204
BpiI GAAGAC 1 cut(s) 29
BpmI CTGGAG 1 cut(s) 97
BpuMI CCSGG 2 cut(s) 39, 40
BsaJI CCNNGG 2 cut(s) 38, 682
Bsc4I CCNNNNNNNGG 2 cut(s) 669, 683
Bse1I ACTGG 6 cut(s) 80, 285, 402, 619, 721, 781
BseBI CCWGG 1 cut(s) 403
BseDI CCNNGG 2 cut(s) 38, 682
BseGI GGATG 1 cut(s) 37
BseLI CCNNNNNNNGG 2 cut(s) 669, 683
BseNI ACTGG 6 cut(s) 80, 285, 402, 619, 721, 781
BseXI GCAGC 3 cut(s) 93, 229, 662
BshFI GGCC 5 cut(s) 183, 401, 540, 662, 698
BsiHKCI CYCGRG 1 cut(s) 38
BsiSI CCGG 1 cut(s) 39
BslI CCNNNNNNNGG 2 cut(s) 669, 683
BsmI GAATGC 1 cut(s) 745
BsnI GGCC 5 cut(s) 183, 401, 540, 662, 698
BsoBI CYCGRG 1 cut(s) 38
Bsp1286I GDGCHC 1 cut(s) 208
Bsp143I GATC 1 cut(s) 470
BspANI GGCC 5 cut(s) 183, 401, 540, 662, 698
BspLI GGNNCC 2 cut(s) 184, 207
BspMAI CTGCAG 1 cut(s) 652
BsrI ACTGG 6 cut(s) 80, 285, 402, 619, 721, 781
BssECI CCNNGG 2 cut(s) 38, 682
BssMI GATC 1 cut(s) 470
BssT1I CCWWGG 1 cut(s) 682
Bst2UI CCWGG 1 cut(s) 403
Bst4CI ACNGT 4 cut(s) 118, 166, 312, 795
Bst6I CTCTTC 1 cut(s) 607
BstC8I GCNNGC 4 cut(s) 181, 696, 757, 782
BstDEI CTNAG 1 cut(s) 461
BstEII GGTNACC 3 cut(s) 374, 497, 716
BstF5I GGATG 1 cut(s) 37
BstKTI GATC 1 cut(s) 473
BstMBI GATC 1 cut(s) 470
BstMWI GCNNNNNNNGC 3 cut(s) 140, 631, 659
BstNI CCWGG 1 cut(s) 403
BstNSI RCATGY 1 cut(s) 224
BstPI GGTNACC 3 cut(s) 374, 497, 716
BstSCI CCNGG 3 cut(s) 37, 38, 401
BstSFI CTRYAG 1 cut(s) 648
BstV1I GCAGC 3 cut(s) 93, 229, 662
BstV2I GAAGAC 1 cut(s) 29
BstX2I RGATCY 1 cut(s) 470
BstYI RGATCY 1 cut(s) 470
BsuRI GGCC 5 cut(s) 183, 401, 540, 662, 698
BtsCI GGATG 1 cut(s) 37
BtsIMutI CAGTG 3 cut(s) 395, 626, 728
Cac8I GCNNGC 4 cut(s) 181, 696, 757, 782
Cfr13I GGNCC 3 cut(s) 182, 538, 661
Cfr9I CCCGGG 1 cut(s) 38
CseI GACGC 2 cut(s) 34, 522
Csp6I GTAC 2 cut(s) 68, 121
CviAII CATG 1 cut(s) 221
CviQI GTAC 2 cut(s) 68, 121
DdeI CTNAG 1 cut(s) 461
DpnI GATC 1 cut(s) 472
DpnII GATC 1 cut(s) 470
Eam1104I CTCTTC 1 cut(s) 607
EarI CTCTTC 1 cut(s) 607
Eco130I CCWWGG 1 cut(s) 682
Eco24I GRGCYC 1 cut(s) 208
Eco88I CYCGRG 1 cut(s) 38
Eco91I GGTNACC 3 cut(s) 374, 497, 716
EcoO109I RGGNCCY 1 cut(s) 661
EcoO65I GGTNACC 3 cut(s) 374, 497, 716
EcoRII CCWGG 1 cut(s) 401
EcoT14I CCWWGG 1 cut(s) 682
EcoT22I ATGCAT 1 cut(s) 145
EcoT38I GRGCYC 1 cut(s) 208
ErhI CCWWGG 1 cut(s) 682
FaeI CATG 1 cut(s) 224
FaiI YATR 8 cut(s) 127, 222, 350, 416, 477, 479, 581, 583
FatI CATG 1 cut(s) 220
Fnu4HI GCNGC 3 cut(s) 82, 218, 651
FokI GGATG 1 cut(s) 44
FriOI GRGCYC 1 cut(s) 208
Fsp4HI GCNGC 3 cut(s) 82, 218, 651
FspBI CTAG 2 cut(s) 107, 552
GluI GCNGC 3 cut(s) 82, 218, 651
GsuI CTGGAG 1 cut(s) 97
HaeIII GGCC 5 cut(s) 183, 401, 540, 662, 698
HapII CCGG 1 cut(s) 39
HgaI GACGC 2 cut(s) 34, 522
Hin1II CATG 1 cut(s) 224
HinfI GANTC 1 cut(s) 263
HpaII CCGG 1 cut(s) 39
HphI GGTGA 4 cut(s) 368, 491, 722, 728
Hpy166II GTNNAC 2 cut(s) 121, 395
Hpy188I TCNGA 2 cut(s) 19, 139
Hpy188III TCNNGA 3 cut(s) 209, 506, 736
Hpy8I GTNNAC 2 cut(s) 121, 395
Hpy99I CGWCG 1 cut(s) 538
HpyAV CCTTC 1 cut(s) 707
HpyCH4III ACNGT 4 cut(s) 118, 166, 312, 795
HpyCH4V TGCA 7 cut(s) 10, 98, 143, 352, 650, 726, 743
HpyF10VI GCNNNNNNNGC 3 cut(s) 140, 631, 659
HpyF3I CTNAG 1 cut(s) 461
Hsp92II CATG 1 cut(s) 224
Kzo9I GATC 1 cut(s) 470
LmnI GCTCC 3 cut(s) 40, 78, 211
Lsp1109I GCAGC 3 cut(s) 93, 229, 662
LweI GCATC 4 cut(s) 22, 130, 152, 204
MaeI CTAG 2 cut(s) 107, 552
MaeIII GTNAC 4 cut(s) 160, 374, 497, 716
MalI GATC 1 cut(s) 472
MboI GATC 1 cut(s) 470
MboII GAAGA 4 cut(s) 34, 164, 563, 594
MfeI CAATTG 2 cut(s) 99, 254
MflI RGATCY 1 cut(s) 470
MhlI GDGCHC 1 cut(s) 208
MluCI AATT 7 cut(s) 99, 241, 254, 334, 545, 688, 785
MnlI CCTC 4 cut(s) 551, 610, 701, 709
Mph1103I ATGCAT 1 cut(s) 145
MseI TTAA 3 cut(s) 276, 314, 729
MslI CAYNNNNRTG 2 cut(s) 619, 750
MspI CCGG 1 cut(s) 39
MspR9I CCNGG 3 cut(s) 39, 40, 403
MunI CAATTG 2 cut(s) 99, 254
Mva1269I GAATGC 1 cut(s) 745
MvaI CCWGG 1 cut(s) 403
MwoI GCNNNNNNNGC 3 cut(s) 140, 631, 659
NciI CCSGG 2 cut(s) 39, 40
NdeII GATC 1 cut(s) 470
NlaIII CATG 1 cut(s) 224
NlaIV GGNNCC 2 cut(s) 184, 207
NmuCI GTSAC 4 cut(s) 160, 374, 497, 716
NsiI ATGCAT 1 cut(s) 145
NspI RCATGY 1 cut(s) 224
OliI CACNNNNGTG 2 cut(s) 619, 750
PctI GAATGC 1 cut(s) 745
PfeI GAWTC 1 cut(s) 263
PkrI GCNGC 3 cut(s) 83, 219, 652
Psp6I CCWGG 1 cut(s) 401
PspEI GGTNACC 3 cut(s) 374, 497, 716
PspGI CCWGG 1 cut(s) 401
PspN4I GGNNCC 2 cut(s) 184, 207
PspPI GGNCC 3 cut(s) 182, 538, 661
PstI CTGCAG 1 cut(s) 652
PsuI RGATCY 1 cut(s) 470
RsaI GTAC 2 cut(s) 69, 122
RsaNI GTAC 2 cut(s) 68, 121
RseI CAYNNNNRTG 2 cut(s) 619, 750
SaqAI TTAA 3 cut(s) 276, 314, 729
SatI GCNGC 3 cut(s) 82, 218, 651
Sau3AI GATC 1 cut(s) 470
Sau96I GGNCC 3 cut(s) 182, 538, 661
ScrFI CCNGG 3 cut(s) 39, 40, 403
SduI GDGCHC 1 cut(s) 208
SfaNI GCATC 4 cut(s) 22, 130, 152, 204
SfcI CTRYAG 1 cut(s) 648
SmaI CCCGGG 1 cut(s) 40
SmiMI CAYNNNNRTG 2 cut(s) 619, 750
Sse9I AATT 7 cut(s) 99, 241, 254, 334, 545, 688, 785
SspMI CTAG 2 cut(s) 107, 552
StyD4I CCNGG 3 cut(s) 37, 38, 401
StyI CCWWGG 1 cut(s) 682
TaaI ACNGT 4 cut(s) 118, 166, 312, 795
TaqI TCGA 2 cut(s) 72, 450
TaqII GACCGA 1 cut(s) 485
TasI AATT 7 cut(s) 99, 241, 254, 334, 545, 688, 785
TatI WGTACW 1 cut(s) 67
TfiI GAWTC 1 cut(s) 263
Tru1I TTAA 3 cut(s) 276, 314, 729
Tru9I TTAA 3 cut(s) 276, 314, 729
TscAI CASTG 3 cut(s) 402, 626, 728
TseFI GTSAC 4 cut(s) 160, 374, 497, 716
TseI GCWGC 3 cut(s) 81, 217, 650
Tsp45I GTSAC 4 cut(s) 160, 374, 497, 716
TspDTI ATGAA 1 cut(s) 600
TspMI CCCGGG 1 cut(s) 38
TspRI CASTG 3 cut(s) 402, 626, 728
XapI RAATTY 2 cut(s) 334, 688
XceI RCATGY 1 cut(s) 224
XmaI CCCGGG 1 cut(s) 38
XspI CTAG 2 cut(s) 107, 552
Zsp2I ATGCAT 1 cut(s) 145
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.