pycom04g02560

lipid-transfer protein DIR1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
N/A
Physical Location & Seq
Forward (+)
2279645 .. 2281258
1614 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1152 bp
ATGATTTCCACACTTCTTTTTACGCTTTCTCCTATATTTTTTCATCTTTTCCGTAGAGAGAAGTGTGGGGAAAAAAAACCCAGTAGGCAAGTAATACTAATAACTATTAAAACATGCAGACAAAAACAAGTGCGTCGAACTTATCTGAATCACAAGTACGGATCCCGTGACACCACAGCATGGCCAACTTTTGGGTCCACGGGACCACCGTCAGCAAATTCCTTTGCTTCTCATTCCCCTATAAAACCCACCAACTTTGGAGCTCCAAAATCCACAACCTTAACGCACCAAACAATTCCTATGAGAAAAGATATGGAAGGTGCTCGTCAAAAGCTGGTCGTAGTTTTGGCTGCTGTGGTTTTGGTTGCAATTGCAGGCAATGGCGGTTTTGTTCATGTTGTTAATGCTCAGAACATATGCAACGTCTCTGTCAGCGATTTGATGACTTGCAGGCCGGCTGTGACTTCTCCGAATCCGGCCGGCACCGCCTACAAAAGCCTGCTGCGCGGCGCTGTCGCATGCCAACCTGAGCTGCCTTTGCTCCTACAAGAACTCCACTTCCCGCCAAGTGCAAGCTTCCCCATCCTGCCAATTGCTAGACAGAGCGACGTACACAAAGGTCTAATCAACAAACATGATTTAGTTACGATTAAGATATGGTTAATCTTTGGATGTGTGCGTTTCTTTTGTCGTTCTAGTTCAATTTGCTATCATGTTTTCGATATCGAATATGTAATATTATTAATAATAGAAATGTTGGTTTTTTTTCTTTTTTTTTTCGTCTCTTTCAATACAAATCTCGGTTTTCACATAAGTGGTTATGTTCAACTTATGGAATATATAATTAAGATTGCAGTCATATATTTCGCATATAACTCTAGTTTTTTTTATATGGAATATCTCCCATCAAAATTTCACAACCTTCAACAATATACACATACATACATACATACATACATACATACATACATATATATATATATATATATGAAACACCATCTCCAATGGTTTCCTAATCCTTCAACAACATACATACATACATACATACATACATACATACATACATACATACATATATATATATATATATATATATATATATATGAAACACCATCTCCATTGGGGCCTAAAATTTTAGCCCGGGATTATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LTP_2

Protein Analysis

384

Amino Acids

44.19

Weight (kDa)

9.4

Isoelectric Point (pI)

46.36

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 482
AccB7I CCANNNNNTGG 2 cut(s) 180, 191
AccII CGCG 1 cut(s) 507
AciI CCGC 4 cut(s) 384, 486, 507, 563
AclWI GGATC 2 cut(s) 156, 169
AcoI YGGCCR 2 cut(s) 182, 477
AcsI RAATTY 3 cut(s) 217, 911, 1131
AfaI GTAC 2 cut(s) 158, 612
AfiI CCNNNNNNNGG 2 cut(s) 180, 191
AgsI TTSAA 5 cut(s) 702, 790, 827, 926, 1023
AleI CACNNNNGTG 1 cut(s) 813
AloI GAACNNNNNNTCC 2 cut(s) 543, 575
AluBI AGCT 4 cut(s) 263, 334, 532, 576
AluI AGCT 4 cut(s) 263, 334, 532, 576
Alw21I GWGCWC 2 cut(s) 265, 325
Alw26I GTCTC 2 cut(s) 430, 787
AlwI GGATC 2 cut(s) 156, 169
Ama87I CYCGRG 1 cut(s) 1140
AoxI GGCC 4 cut(s) 182, 452, 477, 1124
ApeKI GCWGC 3 cut(s) 350, 502, 532
ApoI RAATTY 3 cut(s) 217, 911, 1131
AseI ATTAAT 1 cut(s) 743
AspLEI GCGC 2 cut(s) 507, 512
AspS9I GGNCC 3 cut(s) 195, 203, 1124
AsuC2I CCSGG 2 cut(s) 1141, 1142
AvaI CYCGRG 1 cut(s) 1140
AvaII GGWCC 2 cut(s) 195, 203
BalI TGGCCA 1 cut(s) 184
BamHI GGATCC 1 cut(s) 161
BanI GGYRCC 1 cut(s) 482
BanII GRGCYC 1 cut(s) 265
Bbv12I GWGCWC 2 cut(s) 265, 325
BbvI GCAGC 3 cut(s) 337, 489, 519
BccI CCATC 4 cut(s) 590, 913, 1005, 1120
BcnI CCSGG 2 cut(s) 1141, 1142
BcoDI GTCTC 2 cut(s) 430, 787
BfaI CTAG 3 cut(s) 597, 696, 879
BfoI RGCGCY 1 cut(s) 513
BisI GCNGC 4 cut(s) 351, 503, 508, 533
BlsI GCNGC 4 cut(s) 352, 504, 509, 534
Bme1390I CCNGG 2 cut(s) 1141, 1142
Bme18I GGWCC 2 cut(s) 195, 203
BmeT110I CYCGRG 1 cut(s) 1140
BmgT120I GGNCC 3 cut(s) 195, 203, 1124
BmiI GGNNCC 5 cut(s) 163, 196, 204, 484, 1125
BmrFI CCNGG 2 cut(s) 1141, 1142
BmrI ACTGGG 1 cut(s) 75
BmuI ACTGGG 1 cut(s) 75
BoxI GACNNNNGTC 1 cut(s) 208
Bpu10I CCTNAGC 1 cut(s) 528
BpuMI CCSGG 2 cut(s) 1141, 1142
BsaJI CCNNGG 2 cut(s) 198, 1140
BsaXI ACNNNNNCTCC 8 cut(s) 13, 43, 537, 567, 984, 1014, 1099, 1129
Bsc4I CCNNNNNNNGG 2 cut(s) 180, 191
Bse118I RCCGGY 2 cut(s) 454, 479
Bse1I ACTGG 1 cut(s) 81
Bse3DI GCAATG 1 cut(s) 385
BseDI CCNNGG 2 cut(s) 198, 1140
BseGI GGATG 2 cut(s) 582, 677
BseLI CCNNNNNNNGG 2 cut(s) 180, 191
BseMI GCAATG 1 cut(s) 385
BseMII CTCAG 2 cut(s) 422, 519
BseNI ACTGG 1 cut(s) 81
BseX3I CGGCCG 1 cut(s) 477
BseXI GCAGC 3 cut(s) 337, 489, 519
Bsh1236I CGCG 1 cut(s) 507
Bsh1285I CGRYCG 1 cut(s) 480
BshFI GGCC 4 cut(s) 184, 454, 479, 1126
BshNI GGYRCC 1 cut(s) 482
BsiEI CGRYCG 1 cut(s) 480
BsiHKAI GWGCWC 2 cut(s) 265, 325
BsiHKCI CYCGRG 1 cut(s) 1140
BsiSI CCGG 4 cut(s) 455, 476, 480, 1141
BslFI GGGAC 1 cut(s) 216
BslI CCNNNNNNNGG 2 cut(s) 180, 191
BsmAI GTCTC 2 cut(s) 430, 787
BsmBI CGTCTC 2 cut(s) 430, 787
BsmFI GGGAC 1 cut(s) 216
BsnI GGCC 4 cut(s) 184, 454, 479, 1126
BsoBI CYCGRG 1 cut(s) 1140
Bsp1286I GDGCHC 2 cut(s) 265, 325
Bsp143I GATC 1 cut(s) 161
BspACI CCGC 4 cut(s) 384, 486, 507, 563
BspANI GGCC 4 cut(s) 184, 454, 479, 1126
BspCNI CTCAG 2 cut(s) 421, 520
BspFNI CGCG 1 cut(s) 507
BspLI GGNNCC 5 cut(s) 163, 196, 204, 484, 1125
BspPI GGATC 2 cut(s) 156, 169
BspT107I GGYRCC 1 cut(s) 482
BsrDI GCAATG 1 cut(s) 385
BsrFI RCCGGY 2 cut(s) 454, 479
BsrI ACTGG 1 cut(s) 81
BssAI RCCGGY 2 cut(s) 454, 479
BssECI CCNNGG 2 cut(s) 198, 1140
BssMI GATC 1 cut(s) 161
Bst4CI ACNGT 1 cut(s) 210
BstC8I GCNNGC 7 cut(s) 376, 452, 456, 481, 500, 520, 574
BstDEI CTNAG 2 cut(s) 408, 528
BstDSI CCRYGG 1 cut(s) 198
BstF5I GGATG 2 cut(s) 582, 677
BstFNI CGCG 1 cut(s) 507
BstH2I RGCGCY 1 cut(s) 513
BstHHI GCGC 2 cut(s) 507, 512
BstKTI GATC 1 cut(s) 164
BstMAI GTCTC 2 cut(s) 430, 787
BstMBI GATC 1 cut(s) 161
BstMCI CGRYCG 1 cut(s) 480
BstMWI GCNNNNNNNGC 3 cut(s) 485, 504, 538
BstNSI RCATGY 2 cut(s) 117, 522
BstPAI GACNNNNGTC 1 cut(s) 208
BstSCI CCNGG 2 cut(s) 1139, 1140
BstUI CGCG 1 cut(s) 507
BstV1I GCAGC 3 cut(s) 337, 489, 519
BstX2I RGATCY 1 cut(s) 161
BstYI RGATCY 1 cut(s) 161
BstZI CGGCCG 1 cut(s) 477
BsuRI GGCC 4 cut(s) 184, 454, 479, 1126
BtgI CCRYGG 1 cut(s) 198
BtsCI GGATG 2 cut(s) 582, 677
Cac8I GCNNGC 7 cut(s) 376, 452, 456, 481, 500, 520, 574
CfoI GCGC 2 cut(s) 507, 512
Cfr10I RCCGGY 2 cut(s) 454, 479
Cfr13I GGNCC 3 cut(s) 195, 203, 1124
Cfr9I CCCGGG 1 cut(s) 1140
CseI GACGC 1 cut(s) 122
Csp6I GTAC 2 cut(s) 157, 611
CspCI CAANNNNNGTGG 2 cut(s) 238, 273
CviAII CATG 6 cut(s) 114, 180, 395, 519, 635, 713
CviQI GTAC 2 cut(s) 157, 611
DdeI CTNAG 2 cut(s) 408, 528
DpnI GATC 1 cut(s) 163
DpnII GATC 1 cut(s) 161
EaeI YGGCCR 2 cut(s) 182, 477
EagI CGGCCG 1 cut(s) 477
Ecl136II GAGCTC 1 cut(s) 263
EclXI CGGCCG 1 cut(s) 477
Eco24I GRGCYC 1 cut(s) 265
Eco32I GATATC 1 cut(s) 724
Eco47I GGWCC 2 cut(s) 195, 203
Eco52I CGGCCG 1 cut(s) 477
Eco53kI GAGCTC 1 cut(s) 263
Eco88I CYCGRG 1 cut(s) 1140
EcoICRI GAGCTC 1 cut(s) 263
EcoO109I RGGNCCY 1 cut(s) 1124
EcoRV GATATC 1 cut(s) 724
EcoT38I GRGCYC 1 cut(s) 265
Esp3I CGTCTC 2 cut(s) 430, 787
FaeI CATG 6 cut(s) 117, 183, 398, 522, 638, 716
FaqI GGGAC 1 cut(s) 216
FatI CATG 6 cut(s) 113, 179, 394, 518, 634, 712
FauI CCCGC 1 cut(s) 570
FauNDI CATATG 1 cut(s) 416
Fnu4HI GCNGC 4 cut(s) 351, 503, 508, 533
FokI GGATG 2 cut(s) 569, 684
FriOI GRGCYC 1 cut(s) 265
Fsp4HI GCNGC 4 cut(s) 351, 503, 508, 533
FspBI CTAG 3 cut(s) 597, 696, 879
GlaI GCGC 2 cut(s) 506, 511
GluI GCNGC 4 cut(s) 351, 503, 508, 533
HaeII RGCGCY 1 cut(s) 513
HaeIII GGCC 4 cut(s) 184, 454, 479, 1126
HapII CCGG 4 cut(s) 455, 476, 480, 1141
HgaI GACGC 1 cut(s) 122
HhaI GCGC 2 cut(s) 507, 512
Hin1II CATG 6 cut(s) 117, 183, 398, 522, 638, 716
Hin6I GCGC 2 cut(s) 505, 510
HinP1I GCGC 2 cut(s) 505, 510
HindIII AAGCTT 1 cut(s) 574
HinfI GANTC 2 cut(s) 148, 472
HpaII CCGG 4 cut(s) 455, 476, 480, 1141
Hpy166II GTNNAC 2 cut(s) 198, 613
Hpy188I TCNGA 3 cut(s) 147, 411, 471
Hpy8I GTNNAC 2 cut(s) 198, 613
Hpy99I CGWCG 2 cut(s) 138, 611
HpyAV CCTTC 3 cut(s) 311, 932, 1029
HpyCH4III ACNGT 1 cut(s) 210
HpyCH4IV ACGT 2 cut(s) 423, 609
HpyCH4V TGCA 7 cut(s) 117, 368, 374, 420, 450, 572, 854
HpyF10VI GCNNNNNNNGC 3 cut(s) 485, 504, 538
HpyF3I CTNAG 2 cut(s) 408, 528
HpySE526I ACGT 2 cut(s) 423, 609
Hsp92II CATG 6 cut(s) 117, 183, 398, 522, 638, 716
HspAI GCGC 2 cut(s) 505, 510
KroI GCCGGC 2 cut(s) 454, 479
KroNI GCCGGC 2 cut(s) 456, 481
Kzo9I GATC 1 cut(s) 161
LmnI GCTCC 3 cut(s) 260, 268, 546
Lsp1109I GCAGC 3 cut(s) 337, 489, 519
MaeI CTAG 3 cut(s) 597, 696, 879
MaeII ACGT 2 cut(s) 423, 609
MaeIII GTNAC 3 cut(s) 167, 460, 643
MalI GATC 1 cut(s) 163
MboI GATC 1 cut(s) 161
MfeI CAATTG 2 cut(s) 369, 591
MflI RGATCY 1 cut(s) 161
MhlI GDGCHC 2 cut(s) 265, 325
MlsI TGGCCA 1 cut(s) 184
MluCI AATT 8 cut(s) 217, 294, 369, 591, 702, 843, 911, 1131
MluNI TGGCCA 1 cut(s) 184
Mox20I TGGCCA 1 cut(s) 184
MroNI GCCGGC 2 cut(s) 454, 479
MscI TGGCCA 1 cut(s) 184
MseI TTAA 8 cut(s) 108, 281, 402, 651, 662, 743, 846, 1150
MslI CAYNNNNRTG 1 cut(s) 813
Msp20I TGGCCA 1 cut(s) 184
MspI CCGG 4 cut(s) 455, 476, 480, 1141
MspR9I CCNGG 2 cut(s) 1141, 1142
MteI GCGCNGCGC 1 cut(s) 508
MunI CAATTG 2 cut(s) 369, 591
MvnI CGCG 1 cut(s) 507
MwoI GCNNNNNNNGC 3 cut(s) 485, 504, 538
NaeI GCCGGC 2 cut(s) 456, 481
NciI CCSGG 2 cut(s) 1141, 1142
NdeI CATATG 1 cut(s) 416
NdeII GATC 1 cut(s) 161
NgoMIV GCCGGC 2 cut(s) 454, 479
NlaIII CATG 6 cut(s) 117, 183, 398, 522, 638, 716
NlaIV GGNNCC 5 cut(s) 163, 196, 204, 484, 1125
NmuCI GTSAC 2 cut(s) 167, 460
NspI RCATGY 2 cut(s) 117, 522
OliI CACNNNNGTG 1 cut(s) 813
PaeI GCATGC 1 cut(s) 522
PcsI WCGNNNNNNNCGW 1 cut(s) 206
PdiI GCCGGC 2 cut(s) 456, 481
PfeI GAWTC 2 cut(s) 148, 472
PflMI CCANNNNNTGG 2 cut(s) 180, 191
PkrI GCNGC 4 cut(s) 352, 504, 509, 534
PshAI GACNNNNGTC 1 cut(s) 208
PshBI ATTAAT 1 cut(s) 743
Psp124BI GAGCTC 1 cut(s) 265
PspN4I GGNNCC 5 cut(s) 163, 196, 204, 484, 1125
PspPI GGNCC 3 cut(s) 195, 203, 1124
PsuI RGATCY 1 cut(s) 161
RsaI GTAC 2 cut(s) 158, 612
RsaNI GTAC 2 cut(s) 157, 611
RseI CAYNNNNRTG 1 cut(s) 813
SacI GAGCTC 1 cut(s) 265
SaqAI TTAA 8 cut(s) 108, 281, 402, 651, 662, 743, 846, 1150
SatI GCNGC 4 cut(s) 351, 503, 508, 533
Sau3AI GATC 1 cut(s) 161
Sau96I GGNCC 3 cut(s) 195, 203, 1124
ScrFI CCNGG 2 cut(s) 1141, 1142
SduI GDGCHC 2 cut(s) 265, 325
SinI GGWCC 2 cut(s) 195, 203
SmaI CCCGGG 1 cut(s) 1142
SmiMI CAYNNNNRTG 1 cut(s) 813
SphI GCATGC 1 cut(s) 522
Sse9I AATT 8 cut(s) 217, 294, 369, 591, 702, 843, 911, 1131
SsiI CCGC 4 cut(s) 384, 486, 507, 563
SspI AATATT 1 cut(s) 738
SspMI CTAG 3 cut(s) 597, 696, 879
SstI GAGCTC 1 cut(s) 265
StyD4I CCNGG 2 cut(s) 1139, 1140
TaaI ACNGT 1 cut(s) 210
TaiI ACGT 2 cut(s) 426, 612
TaqI TCGA 3 cut(s) 136, 720, 726
TasI AATT 8 cut(s) 217, 294, 369, 591, 702, 843, 911, 1131
TauI GCSGC 1 cut(s) 510
TfiI GAWTC 2 cut(s) 148, 472
Tru1I TTAA 8 cut(s) 108, 281, 402, 651, 662, 743, 846, 1150
Tru9I TTAA 8 cut(s) 108, 281, 402, 651, 662, 743, 846, 1150
TseFI GTSAC 2 cut(s) 167, 460
TseI GCWGC 3 cut(s) 350, 502, 532
Tsp45I GTSAC 2 cut(s) 167, 460
TspDTI ATGAA 4 cut(s) 32, 383, 1004, 1119
TspGWI ACGGA 2 cut(s) 41, 174
TspMI CCCGGG 1 cut(s) 1140
Van91I CCANNNNNTGG 2 cut(s) 180, 191
VpaK11BI GGWCC 2 cut(s) 195, 203
VspI ATTAAT 1 cut(s) 743
XapI RAATTY 3 cut(s) 217, 911, 1131
XceI RCATGY 2 cut(s) 117, 522
XmaI CCCGGG 1 cut(s) 1140
XspI CTAG 3 cut(s) 597, 696, 879
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.