pycom04g03650

Belongs to the disease resistance NB-LRR family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
N/A
Physical Location & Seq
Reverse (-)
3282489 .. 3286269
3781 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 642 bp
ATGACAGGAAAATTCTATGACATCATTTTGCCCAAAGCTGAGCAAGTCGGTTTCTTTCATATTAACAAAAATATTGCATCGTCATCATCATCAACTGACCGTGACCATGTTCATCAGAGTCAGACACCTCCTCCTCGGGTTGCAGTTCGTAGGGTTGCCGAGTACCTGGGCCTAAGATGGACCTTCGTGGATGCTCTTCCTTCATCGGTTCGTAACCTCATCCATCTGCAGACCTTGGACCTCAAGCATACTTATATCCGTTCTCTCCCAGTTTCCTTTCAGAATATGGATTTCTTGCGGCATCTATACTTGAATGATATTCGTGTGGGTAGGATTTGGATGTTGGATAGTGGCCAAATTAGGCTAGGAAACAAAACAATAGTGATGAGGGAACATCATGATGAGGATTATGTTTCCTTGTTTTGTGGCTTGTTTTGTGGCTTAAATTGCTCCATGTTTTGTGGCTTTTTCAAAGCCATGTTTTGTGGGAGAGGCGGTGGATTCAGAGTAAAGCATCCAAAGAGATGGCTTTTGGGAAAATTCTACGAGGATGTAGCAAGGAAGAGATGTAAAGCATCCAAAGAGATGGTGAGAGCATTTGGCTCTACCATGGGTAAGGGTGAGAGAAATCAAGAGAGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04014 ko04626 map04014 map04626
Pfam Domains
Protein Families
LRR_8 NB-ARC

Protein Analysis

214

Amino Acids

24.57

Weight (kDa)

9.75

Isoelectric Point (pI)

52.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
LRR_14 PF23598 55 - 108 1.1e-10 Leucine-rich repeat region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 298, 495
AcoI YGGCCR 1 cut(s) 352
AcsI RAATTY 2 cut(s) 11, 539
AfaI GTAC 1 cut(s) 164
AgsI TTSAA 2 cut(s) 313, 472
AjnI CCWGG 1 cut(s) 165
AluBI AGCT 2 cut(s) 38, 639
AluI AGCT 2 cut(s) 38, 639
Ama87I CYCGRG 1 cut(s) 135
AoxI GGCC 2 cut(s) 169, 352
ApoI RAATTY 2 cut(s) 11, 539
AspS9I GGNCC 3 cut(s) 169, 180, 238
AsuHPI GGTGA 2 cut(s) 601, 632
AvaI CYCGRG 1 cut(s) 135
AvaII GGWCC 2 cut(s) 180, 238
BalI TGGCCA 1 cut(s) 354
BccI CCATC 4 cut(s) 171, 231, 519, 580
BciT130I CCWGG 1 cut(s) 167
BfaI CTAG 2 cut(s) 365, 640
BfmI CTRYAG 1 cut(s) 227
BisI GCNGC 1 cut(s) 299
BlpI GCTNAGC 1 cut(s) 39
BlsI GCNGC 1 cut(s) 300
Bme1390I CCNGG 1 cut(s) 167
Bme18I GGWCC 2 cut(s) 180, 238
BmeT110I CYCGRG 1 cut(s) 135
BmgT120I GGNCC 3 cut(s) 169, 180, 238
BmrFI CCNGG 1 cut(s) 167
BmrI ACTGGG 1 cut(s) 263
BmsI GCATC 5 cut(s) 86, 181, 310, 523, 584
BmuI ACTGGG 1 cut(s) 263
Bpu1102I GCTNAGC 1 cut(s) 39
BpuEI CTTGAG 1 cut(s) 227
BsaJI CCNNGG 4 cut(s) 134, 166, 234, 609
BsaXI ACNNNNNCTCC 4 cut(s) 115, 145, 481, 511
Bse1I ACTGG 1 cut(s) 269
BseBI CCWGG 1 cut(s) 167
BseDI CCNNGG 4 cut(s) 134, 166, 234, 609
BseGI GGATG 6 cut(s) 196, 219, 345, 514, 556, 575
BseMII CTCAG 1 cut(s) 30
BseNI ACTGG 1 cut(s) 269
BseRI GAGGAG 2 cut(s) 120, 123
BshFI GGCC 2 cut(s) 171, 354
BsiHKCI CYCGRG 1 cut(s) 135
BsnI GGCC 2 cut(s) 171, 354
BsoBI CYCGRG 1 cut(s) 135
Bsp1720I GCTNAGC 1 cut(s) 39
Bsp19I CCATGG 1 cut(s) 609
BspACI CCGC 2 cut(s) 298, 495
BspANI GGCC 2 cut(s) 171, 354
BspCNI CTCAG 1 cut(s) 31
BspHI TCATGA 1 cut(s) 397
BspMAI CTGCAG 1 cut(s) 231
BspQI GCTCTTC 1 cut(s) 201
BsrI ACTGG 1 cut(s) 269
BssECI CCNNGG 4 cut(s) 134, 166, 234, 609
BssT1I CCWWGG 2 cut(s) 234, 609
Bst2UI CCWGG 1 cut(s) 167
Bst4CI ACNGT 1 cut(s) 101
Bst6I CTCTTC 2 cut(s) 201, 557
BstDEI CTNAG 2 cut(s) 39, 173
BstDSI CCRYGG 1 cut(s) 609
BstF5I GGATG 6 cut(s) 196, 219, 345, 514, 556, 575
BstMWI GCNNNNNNNGC 1 cut(s) 447
BstNI CCWGG 1 cut(s) 167
BstSCI CCNGG 1 cut(s) 165
BstSFI CTRYAG 1 cut(s) 227
BstXI CCANNNNNNTGG 2 cut(s) 525, 586
BsuRI GGCC 2 cut(s) 171, 354
BtgI CCRYGG 1 cut(s) 609
BtsCI GGATG 6 cut(s) 196, 219, 345, 514, 556, 575
CciI TCATGA 1 cut(s) 397
Cfr13I GGNCC 3 cut(s) 169, 180, 238
Csp6I GTAC 1 cut(s) 163
CviAII CATG 5 cut(s) 107, 398, 454, 478, 610
CviQI GTAC 1 cut(s) 163
DdeI CTNAG 2 cut(s) 39, 173
EaeI YGGCCR 1 cut(s) 352
Eam1104I CTCTTC 2 cut(s) 201, 557
EarI CTCTTC 2 cut(s) 201, 557
Eco130I CCWWGG 2 cut(s) 234, 609
Eco47I GGWCC 2 cut(s) 180, 238
Eco88I CYCGRG 1 cut(s) 135
EcoRII CCWGG 1 cut(s) 165
EcoT14I CCWWGG 2 cut(s) 234, 609
ErhI CCWWGG 2 cut(s) 234, 609
FaeI CATG 5 cut(s) 110, 401, 457, 481, 613
FatI CATG 5 cut(s) 106, 397, 453, 477, 609
Fnu4HI GCNGC 1 cut(s) 299
FokI GGATG 6 cut(s) 203, 206, 352, 501, 562, 563
Fsp4HI GCNGC 1 cut(s) 299
FspBI CTAG 2 cut(s) 365, 640
GluI GCNGC 1 cut(s) 299
HaeIII GGCC 2 cut(s) 171, 354
Hin1II CATG 5 cut(s) 110, 401, 457, 481, 613
HinfI GANTC 2 cut(s) 118, 501
HphI GGTGA 2 cut(s) 601, 632
Hpy188I TCNGA 4 cut(s) 117, 123, 282, 506
Hpy188III TCNNGA 2 cut(s) 398, 632
HpyAV CCTTC 2 cut(s) 193, 210
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4V TGCA 3 cut(s) 77, 143, 229
HpyF10VI GCNNNNNNNGC 1 cut(s) 447
HpyF3I CTNAG 2 cut(s) 39, 173
Hsp92II CATG 5 cut(s) 110, 401, 457, 481, 613
LguI GCTCTTC 1 cut(s) 201
LmnI GCTCC 1 cut(s) 455
LpnPI CCDG 3 cut(s) 152, 179, 282
LweI GCATC 5 cut(s) 86, 181, 310, 523, 584
MaeI CTAG 2 cut(s) 365, 640
MaeIII GTNAC 2 cut(s) 101, 212
MboII GAAGA 2 cut(s) 188, 574
MlsI TGGCCA 1 cut(s) 354
MluCI AATT 4 cut(s) 11, 357, 445, 539
MluNI TGGCCA 1 cut(s) 354
MlyI GAGTC 1 cut(s) 127
MmeI TCCRAC 1 cut(s) 324
MnlI CCTC 9 cut(s) 138, 141, 144, 227, 251, 381, 397, 485, 541
Mox20I TGGCCA 1 cut(s) 354
MscI TGGCCA 1 cut(s) 354
MseI TTAA 2 cut(s) 63, 443
MslI CAYNNNNRTG 1 cut(s) 399
Msp20I TGGCCA 1 cut(s) 354
MspR9I CCNGG 1 cut(s) 167
MvaI CCWGG 1 cut(s) 167
MwoI GCNNNNNNNGC 1 cut(s) 447
NcoI CCATGG 1 cut(s) 609
NlaIII CATG 5 cut(s) 110, 401, 457, 481, 613
NmeAIII GCCGAG 1 cut(s) 184
NmuCI GTSAC 1 cut(s) 101
PagI TCATGA 1 cut(s) 397
PciSI GCTCTTC 1 cut(s) 201
PfeI GAWTC 1 cut(s) 501
PkrI GCNGC 1 cut(s) 300
PleI GAGTC 1 cut(s) 126
PpsI GAGTC 1 cut(s) 126
Psp6I CCWGG 1 cut(s) 165
PspGI CCWGG 1 cut(s) 165
PspPI GGNCC 3 cut(s) 169, 180, 238
PstI CTGCAG 1 cut(s) 231
RsaI GTAC 1 cut(s) 164
RsaNI GTAC 1 cut(s) 163
RseI CAYNNNNRTG 1 cut(s) 399
SapI GCTCTTC 1 cut(s) 201
SaqAI TTAA 2 cut(s) 63, 443
SatI GCNGC 1 cut(s) 299
Sau96I GGNCC 3 cut(s) 169, 180, 238
SchI GAGTC 1 cut(s) 127
ScrFI CCNGG 1 cut(s) 167
SetI ASST 8 cut(s) 40, 130, 168, 185, 219, 236, 243, 641
SfaNI GCATC 5 cut(s) 86, 181, 310, 523, 584
SfcI CTRYAG 1 cut(s) 227
SinI GGWCC 2 cut(s) 180, 238
SmiMI CAYNNNNRTG 1 cut(s) 399
SmlI CTYRAG 1 cut(s) 242
SmoI CTYRAG 1 cut(s) 242
Sse9I AATT 4 cut(s) 11, 357, 445, 539
SsiI CCGC 2 cut(s) 298, 495
SspI AATATT 1 cut(s) 73
SspMI CTAG 2 cut(s) 365, 640
StyD4I CCNGG 1 cut(s) 165
StyI CCWWGG 2 cut(s) 234, 609
TaaI ACNGT 1 cut(s) 101
TasI AATT 4 cut(s) 11, 357, 445, 539
TauI GCSGC 1 cut(s) 301
TfiI GAWTC 1 cut(s) 501
Tru1I TTAA 2 cut(s) 63, 443
Tru9I TTAA 2 cut(s) 63, 443
TseFI GTSAC 1 cut(s) 101
Tsp45I GTSAC 1 cut(s) 101
TspDTI ATGAA 3 cut(s) 47, 101, 192
TspGWI ACGGA 1 cut(s) 248
VpaK11BI GGWCC 2 cut(s) 180, 238
XapI RAATTY 2 cut(s) 11, 539
XspI CTAG 2 cut(s) 365, 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.