pycom04g03960

metalloendoproteinase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
N/A
Physical Location & Seq
Forward (+)
3539687 .. 3542500
2814 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 2136 bp
ATGAAAACTAAGTTGATTACTATATTAAATACGAGAGGAAATAAAATAGTTAAACCCATCATCATCTTCCCCACATCTTCATGCCTGAATTTTCTCTTTCTTCTTTCTCCTCCTCTTCCTCCATTTTTTGTCTCTAATTACAGAATCCTCAGTCCACAATATTCAGAACCCCCACCTCACACACATCAACAAAAAAACAAAAAACAAAAAAAAACCCAAAAAACCAATTTTCACAGAGCGGAAGTGATAAGGATATCGTTCCTTCCATTCGGTTTCGGTAGAAGTTTTGGCCCGCTGCGTTCGTTATTCCAAATCACGGTATCGTTGAGGCATAGTTCTAGATCGCGATGGTGGGGTGTATTTGGGTATTTTGGGTGGAAAATTTTGATTCCTGATGTAACCTTCTACCTGGAGTCCAATTACTACCCCTATAGAGTGGGTGGGCCCTACATAATTTATGATTCCTTACTTGCTGGTAAACGCCATAATACTTTGCGAGGAGTGCAATTCCGTTTCTCCACATTCCAACCCCAATTGGTAACACACTTCTGCAATCATCCCAGTAGACTCTCAAACCAACGGATCAAAAGACAAATGAACTCATCCAAAATCTATAGAAAACGAAACCCTCAATCCATAATTGATAGAGTATGGGATGTGAATGTAGTATTGGAAAGTAAGTGGGGTTTATTAAGCACGTGGAGTGTGGTCAACAAAATATGTAGTGTGTATAAAACAACCCATTACGTATTACAGGCGACAAAAACACGATTCCTTATATCAAACAATAAACACATAATTAACTTGGTTCAGGAATATTATCCAGCACATGGGAATAATATATATCAACACAATTATTCTGAACCAAGTTTTTGGAGTAAAAAAAAACAAAAAAAAACATTCTATCAGAGTAAAATAACACAAGTTGGCGATTATGAAATACATTATGTACAGAATATTATTATCCAGGACATACATGCATGTGAACAATCTCTGACCATTATTTATGATGTCCATTTTTTACCATTCATTACATTTAGCCAAATTCACTCAAGCAATGTGCTCAATTCTCTGCACTTAAGTTCGAATTATGTTTTCCGTAGTTTAAATCAATTTAGTGTGCATTATCTTAGTATTTGTAAAATATACGTATATAATCGGATTAGGGCAACTGCAAGATTAATCTTGCTACAATATTCTACCCTAGCCCTCCAAATATGTGGCGTTTGCAACATTCAAAAAATAGTGAAAATGGCCTCAAAAGCCCTCTCTCTCATCCTTTTCTCTCTTCTCTCTTCTGCAACTTCATCAACATCTCCGTTTGAGTTCTTAGAGCATCTGAAGGGGGGTCACAAGGGTGACCACATTGAAGGCATCCATGACCTCAAGCTATACCTCGAAAAATTCGGCTACTTAAGCTATGCCCATGCCAAGAACAACCCTCATGCCAATGACGATGATTTTGACAACCTACTCGAGTTAGCCGTCAAGACATACCAGCTCAATTACCACCTCAACACCACCGGAACATTGGATGGCGAAACGGTGTCAAAGATGATGATGCCTCGTTGTGGTAAAAAGCACCATCAACCTGACCCCGACCATGACCACCACAACCACGGTTCCAGTGTGCATGCAGTCTCTCACTATTCTTTCTTCAAGGGAAGGCTAAGATGGCCAACTTCTAAGCATCATCTCACATATGGATTTCTTCCAAGCACCCCGACGGAAGCCATGGGACCGGTTTCACATGCGTTTCGAACATGGGCGTCAAACACGCACTTCAGATTTAGTCGTTCCAGAAACTACAAAAGGGCGGACATCAAGATTAGTTTCGAATACAGCGATCATGGAGATGGGAGCCCATTTGATGGGGAAGGTGGGGTTTTGGCCCATGCTTATGCTCCAACAAATGGAAGATTCCACTACGACGCCGATGAGCCATGGTCTGTGGGTGCTTTGGAAGGTTATTACGACTTGGAGACGGTGGCTTTGCATGAGATCGGACACCTTCTTGGACTAGGGCATAGCTCAGTTGAGGGAGCCATCATGTACCCTTACATATCTTCTGGAGCCACCAAGCTGACTTTGCATGACGACGACATTCAAGGGATTAAAGTTCTATACAAGTCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PG_binding_1 Peptidase_M10

Protein Analysis

712

Amino Acids

81.84

Weight (kDa)

9.55

Isoelectric Point (pI)

48.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PG_binding_1 PF01471 458 - 519 4.7e-09 Putative peptidoglycan binding domain
Peptidase_M10 PF00413 559 - 709 1.2e-47 Matrixin
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 3 cut(s) 830, 1871, 1913
AccBSI CCGCTC 1 cut(s) 239
AccI GTMKAC 1 cut(s) 565
AccII CGCG 1 cut(s) 346
AciI CCGC 3 cut(s) 239, 293, 1817
AclWI GGATC 1 cut(s) 590
AcoI YGGCCR 1 cut(s) 1676
AcsI RAATTY 4 cut(s) 88, 381, 1044, 1403
AcuI CTGAAG 2 cut(s) 1361, 1768
AcvI CACGTG 1 cut(s) 699
AcyI GRCGYC 2 cut(s) 1769, 1932
AfaI GTAC 2 cut(s) 951, 2054
AfiI CCNNNNNNNGG 5 cut(s) 316, 830, 1571, 1871, 1913
AflII CTTAAG 2 cut(s) 1078, 1414
AgeI ACCGGT 1 cut(s) 1741
AgsI TTSAA 4 cut(s) 1238, 1370, 1660, 2108
AjnI CCWGG 2 cut(s) 408, 966
AjuI GAANNNNNNNTTGG 2 cut(s) 653, 685
AleI CACNNNNGTG 1 cut(s) 1356
AluBI AGCT 5 cut(s) 1390, 1419, 1501, 2031, 2083
AluI AGCT 5 cut(s) 1390, 1419, 1501, 2031, 2083
Alw21I GWGCWC 1 cut(s) 1065
Alw26I GTCTC 3 cut(s) 136, 1645, 1976
AlwI GGATC 1 cut(s) 590
Ama87I CYCGRG 1 cut(s) 1475
AoxI GGCC 5 cut(s) 289, 443, 1254, 1676, 1890
ApaI GGGCCC 1 cut(s) 447
ApeKI GCWGC 1 cut(s) 295
ApoI RAATTY 4 cut(s) 88, 381, 1044, 1403
ArsI GACNNNNNNTTYG 4 cut(s) 1445, 1477, 1975, 2007
AseI ATTAAT 1 cut(s) 1181
AsiGI ACCGGT 1 cut(s) 1741
AspS9I GGNCC 5 cut(s) 290, 443, 444, 1739, 1891
AsuHPI GGTGA 1 cut(s) 1370
AsuII TTCGAA 3 cut(s) 1085, 1759, 1836
AvaI CYCGRG 1 cut(s) 1475
AvaII GGWCC 1 cut(s) 1739
BaeGI GKGCMC 1 cut(s) 447
BalI TGGCCA 1 cut(s) 1678
BanII GRGCYC 2 cut(s) 447, 1865
BbrPI CACGTG 1 cut(s) 699
Bbv12I GWGCWC 1 cut(s) 1065
BbvI GCAGC 1 cut(s) 282
BccI CCATC 8 cut(s) 65, 342, 1529, 1593, 1668, 1850, 1865, 2054
BceAI ACGGC 1 cut(s) 1469
BciT130I CCWGG 2 cut(s) 410, 968
BcoDI GTCTC 3 cut(s) 136, 1645, 1976
BfaI CTAG 3 cut(s) 339, 1205, 2021
BfmI CTRYAG 2 cut(s) 430, 613
BfrI CTTAAG 2 cut(s) 1078, 1414
BisI GCNGC 1 cut(s) 296
BlsI GCNGC 1 cut(s) 297
Bme1390I CCNGG 2 cut(s) 410, 968
Bme18I GGWCC 1 cut(s) 1739
BmeT110I CYCGRG 1 cut(s) 1475
BmgT120I GGNCC 5 cut(s) 290, 443, 444, 1739, 1891
BmiI GGNNCC 6 cut(s) 445, 1624, 1740, 1862, 2044, 2074
BmrFI CCNGG 2 cut(s) 410, 968
BmrI ACTGGG 1 cut(s) 555
BmsI GCATC 4 cut(s) 1345, 1383, 1551, 1699
BmuI ACTGGG 1 cut(s) 555
BpmI CTGGAG 2 cut(s) 431, 2091
Bpu14I TTCGAA 3 cut(s) 1085, 1759, 1836
BpuEI CTTGAG 2 cut(s) 1036, 1370
BsaAI YACGTR 3 cut(s) 699, 748, 1150
BsaHI GRCGYC 2 cut(s) 1769, 1932
BsaJI CCNNGG 3 cut(s) 1618, 1734, 1943
BsaWI WCCGGW 2 cut(s) 1523, 1741
Bsc4I CCNNNNNNNGG 5 cut(s) 316, 830, 1571, 1871, 1913
Bse118I RCCGGY 1 cut(s) 1741
Bse1I ACTGG 2 cut(s) 561, 1626
Bse3DI GCAATG 1 cut(s) 1063
BseBI CCWGG 2 cut(s) 410, 968
BseDI CCNNGG 3 cut(s) 1618, 1734, 1943
BseGI GGATG 6 cut(s) 556, 602, 661, 1275, 1374, 1540
BseLI CCNNNNNNNGG 5 cut(s) 316, 830, 1571, 1871, 1913
BseMI GCAATG 1 cut(s) 1063
BseMII CTCAG 2 cut(s) 163, 2046
BseNI ACTGG 2 cut(s) 561, 1626
BseRI GAGGAG 3 cut(s) 99, 102, 513
BseSI GKGCMC 1 cut(s) 447
BseXI GCAGC 1 cut(s) 282
BsgI GTGCAG 1 cut(s) 1058
Bsh1236I CGCG 1 cut(s) 346
BshFI GGCC 5 cut(s) 291, 445, 1256, 1678, 1892
BshTI ACCGGT 1 cut(s) 1741
BsiHKAI GWGCWC 1 cut(s) 1065
BsiHKCI CYCGRG 1 cut(s) 1475
BsiSI CCGG 2 cut(s) 1524, 1742
BslFI GGGAC 1 cut(s) 1752
BslI CCNNNNNNNGG 5 cut(s) 316, 830, 1571, 1871, 1913
BsmAI GTCTC 3 cut(s) 136, 1645, 1976
BsmBI CGTCTC 1 cut(s) 1976
BsmFI GGGAC 1 cut(s) 1752
BsnI GGCC 5 cut(s) 291, 445, 1256, 1678, 1892
BsoBI CYCGRG 1 cut(s) 1475
Bsp119I TTCGAA 3 cut(s) 1085, 1759, 1836
Bsp120I GGGCCC 1 cut(s) 443
Bsp1286I GDGCHC 3 cut(s) 447, 1065, 1865
Bsp1407I TGTACA 1 cut(s) 949
Bsp143I GATC 4 cut(s) 341, 582, 1846, 2001
Bsp19I CCATGG 2 cut(s) 1734, 1943
Bsp68I TCGCGA 1 cut(s) 346
BspACI CCGC 3 cut(s) 239, 293, 1817
BspANI GGCC 5 cut(s) 291, 445, 1256, 1678, 1892
BspCNI CTCAG 2 cut(s) 162, 2045
BspFNI CGCG 1 cut(s) 346
BspLI GGNNCC 6 cut(s) 445, 1624, 1740, 1862, 2044, 2074
BspPI GGATC 1 cut(s) 590
BspT104I TTCGAA 3 cut(s) 1085, 1759, 1836
BspTI CTTAAG 2 cut(s) 1078, 1414
BsrBI CCGCTC 1 cut(s) 239
BsrDI GCAATG 1 cut(s) 1063
BsrFI RCCGGY 1 cut(s) 1741
BsrGI TGTACA 1 cut(s) 949
BsrI ACTGG 2 cut(s) 561, 1626
BssAI RCCGGY 1 cut(s) 1741
BssECI CCNNGG 3 cut(s) 1618, 1734, 1943
BssMI GATC 4 cut(s) 341, 582, 1846, 2001
BssNI GRCGYC 2 cut(s) 1769, 1932
BssT1I CCWWGG 2 cut(s) 1734, 1943
Bst2UI CCWGG 2 cut(s) 410, 968
Bst4CI ACNGT 4 cut(s) 319, 1546, 1622, 1987
Bst6I CTCTTC 3 cut(s) 120, 1293, 1300
BstACI GRCGYC 2 cut(s) 1769, 1932
BstAFI CTTAAG 2 cut(s) 1078, 1414
BstAUI TGTACA 1 cut(s) 949
BstBAI YACGTR 3 cut(s) 699, 748, 1150
BstBI TTCGAA 3 cut(s) 1085, 1759, 1836
BstC8I GCNNGC 2 cut(s) 293, 1635
BstDEI CTNAG 7 cut(s) 9, 149, 1130, 1330, 1670, 1686, 2032
BstDSI CCRYGG 3 cut(s) 1618, 1734, 1943
BstEII GGTNACC 1 cut(s) 1358
BstF5I GGATG 6 cut(s) 556, 602, 661, 1275, 1374, 1540
BstFNI CGCG 1 cut(s) 346
BstKTI GATC 4 cut(s) 344, 585, 1849, 2004
BstMAI GTCTC 3 cut(s) 136, 1645, 1976
BstMBI GATC 4 cut(s) 341, 582, 1846, 2001
BstMWI GCNNNNNNNGC 5 cut(s) 502, 1262, 1416, 1675, 2089
BstNI CCWGG 2 cut(s) 410, 968
BstNSI RCATGY 4 cut(s) 980, 984, 1637, 1754
BstPI GGTNACC 1 cut(s) 1358
BstSCI CCNGG 2 cut(s) 408, 966
BstSFI CTRYAG 2 cut(s) 430, 613
BstSLI GKGCMC 1 cut(s) 447
BstSNI TACGTA 2 cut(s) 748, 1150
BstUI CGCG 1 cut(s) 346
BstV1I GCAGC 1 cut(s) 282
BstXI CCANNNNNNTGG 2 cut(s) 873, 1220
BsuRI GGCC 5 cut(s) 291, 445, 1256, 1678, 1892
BtgI CCRYGG 3 cut(s) 1618, 1734, 1943
BtgZI GCGATG 1 cut(s) 361
BtsCI GGATG 6 cut(s) 556, 602, 661, 1275, 1374, 1540
BtsIMutI CAGTG 1 cut(s) 1633
BtuMI TCGCGA 1 cut(s) 346
Cac8I GCNNGC 2 cut(s) 293, 1635
Cfr10I RCCGGY 1 cut(s) 1741
Cfr13I GGNCC 5 cut(s) 290, 443, 444, 1739, 1891
CseI GACGC 2 cut(s) 1758, 1940
Csp6I GTAC 2 cut(s) 950, 2053
CspAI ACCGGT 1 cut(s) 1741
CviQI GTAC 2 cut(s) 950, 2053
DdeI CTNAG 7 cut(s) 9, 149, 1130, 1330, 1670, 1686, 2032
DpnI GATC 4 cut(s) 343, 584, 1848, 2003
DpnII GATC 4 cut(s) 341, 582, 1846, 2001
DraI TTTAAA 1 cut(s) 1107
EaeI YGGCCR 1 cut(s) 1676
Eam1104I CTCTTC 3 cut(s) 120, 1293, 1300
EarI CTCTTC 3 cut(s) 120, 1293, 1300
EciI GGCGGA 1 cut(s) 1832
Eco105I TACGTA 2 cut(s) 748, 1150
Eco130I CCWWGG 2 cut(s) 1734, 1943
Eco24I GRGCYC 2 cut(s) 447, 1865
Eco32I GATATC 1 cut(s) 255
Eco47I GGWCC 1 cut(s) 1739
Eco57I CTGAAG 2 cut(s) 1361, 1768
Eco72I CACGTG 1 cut(s) 699
Eco88I CYCGRG 1 cut(s) 1475
Eco91I GGTNACC 1 cut(s) 1358
EcoO109I RGGNCCY 1 cut(s) 444
EcoO65I GGTNACC 1 cut(s) 1358
EcoRII CCWGG 2 cut(s) 408, 966
EcoRV GATATC 1 cut(s) 255
EcoT14I CCWWGG 2 cut(s) 1734, 1943
EcoT22I ATGCAT 1 cut(s) 982
EcoT38I GRGCYC 2 cut(s) 447, 1865
ErhI CCWWGG 2 cut(s) 1734, 1943
Esp3I CGTCTC 1 cut(s) 1976
FaqI GGGAC 1 cut(s) 1752
FauI CCCGC 1 cut(s) 300
FauNDI CATATG 1 cut(s) 1702
FblI GTMKAC 1 cut(s) 565
Fnu4HI GCNGC 1 cut(s) 296
FokI GGATG 6 cut(s) 543, 589, 668, 1262, 1361, 1547
FriOI GRGCYC 2 cut(s) 447, 1865
Fsp4HI GCNGC 1 cut(s) 296
FspBI CTAG 3 cut(s) 339, 1205, 2021
GluI GCNGC 1 cut(s) 296
GsuI CTGGAG 2 cut(s) 431, 2091
HaeIII GGCC 5 cut(s) 291, 445, 1256, 1678, 1892
HapII CCGG 2 cut(s) 1524, 1742
HgaI GACGC 2 cut(s) 1758, 1940
Hin1I GRCGYC 2 cut(s) 1769, 1932
HincII GTYRAC 1 cut(s) 712
HindII GTYRAC 1 cut(s) 712
HinfI GANTC 7 cut(s) 144, 388, 413, 461, 567, 771, 1920
HpaII CCGG 2 cut(s) 1524, 1742
HphI GGTGA 1 cut(s) 1370
Hpy166II GTNNAC 5 cut(s) 155, 479, 566, 712, 986
Hpy188I TCNGA 8 cut(s) 166, 862, 909, 996, 1161, 1341, 1787, 2006
Hpy188III TCNNGA 9 cut(s) 339, 345, 392, 812, 1489, 1800, 1825, 2070, 2133
Hpy8I GTNNAC 5 cut(s) 155, 479, 566, 712, 986
Hpy99I CGWCG 3 cut(s) 1729, 1934, 2102
HpyAV CCTTC 8 cut(s) 272, 412, 1336, 1364, 1659, 1871, 1958, 2021
HpyCH4III ACNGT 4 cut(s) 319, 1546, 1622, 1987
HpyCH4IV ACGT 3 cut(s) 698, 747, 1149
HpyF10VI GCNNNNNNNGC 5 cut(s) 502, 1262, 1416, 1675, 2089
HpyF3I CTNAG 7 cut(s) 9, 149, 1130, 1330, 1670, 1686, 2032
HpySE526I ACGT 3 cut(s) 698, 747, 1149
Hsp92I GRCGYC 2 cut(s) 1769, 1932
Kzo9I GATC 4 cut(s) 341, 582, 1846, 2001
LmnI GCTCC 4 cut(s) 1860, 1909, 2042, 2072
Lsp1109I GCAGC 1 cut(s) 282
LweI GCATC 4 cut(s) 1345, 1383, 1551, 1699
MaeI CTAG 3 cut(s) 339, 1205, 2021
MaeII ACGT 3 cut(s) 698, 747, 1149
MaeIII GTNAC 4 cut(s) 397, 538, 1349, 1358
MalI GATC 4 cut(s) 343, 584, 1848, 2003
MbiI CCGCTC 1 cut(s) 239
MboI GATC 4 cut(s) 341, 582, 1846, 2001
MfeI CAATTG 1 cut(s) 533
MhlI GDGCHC 3 cut(s) 447, 1065, 1865
MlsI TGGCCA 1 cut(s) 1678
MluNI TGGCCA 1 cut(s) 1678
MlyI GAGTC 2 cut(s) 422, 561
MmeI TCCRAC 2 cut(s) 550, 1931
Mox20I TGGCCA 1 cut(s) 1678
Mph1103I ATGCAT 1 cut(s) 982
MscI TGGCCA 1 cut(s) 1678
MseI TTAA 9 cut(s) 26, 51, 692, 801, 1079, 1106, 1181, 1415, 2115
MslI CAYNNNNRTG 6 cut(s) 79, 981, 1356, 1449, 1854, 1899
Msp20I TGGCCA 1 cut(s) 1678
MspA1I CMGCKG 1 cut(s) 295
MspCI CTTAAG 2 cut(s) 1078, 1414
MspI CCGG 2 cut(s) 1524, 1742
MspR9I CCNGG 2 cut(s) 410, 968
MunI CAATTG 1 cut(s) 533
MvaI CCWGG 2 cut(s) 410, 968
MvnI CGCG 1 cut(s) 346
MwoI GCNNNNNNNGC 5 cut(s) 502, 1262, 1416, 1675, 2089
NcoI CCATGG 2 cut(s) 1734, 1943
NdeI CATATG 1 cut(s) 1702
NdeII GATC 4 cut(s) 341, 582, 1846, 2001
NlaIV GGNNCC 6 cut(s) 445, 1624, 1740, 1862, 2044, 2074
NmuCI GTSAC 2 cut(s) 1349, 1358
NruI TCGCGA 1 cut(s) 346
NsiI ATGCAT 1 cut(s) 982
NspI RCATGY 4 cut(s) 980, 984, 1637, 1754
NspV TTCGAA 3 cut(s) 1085, 1759, 1836
OliI CACNNNNGTG 1 cut(s) 1356
PaeI GCATGC 1 cut(s) 1637
PaeR7I CTCGAG 1 cut(s) 1475
PcsI WCGNNNNNNNCGW 2 cut(s) 1482, 1842
PfeI GAWTC 5 cut(s) 144, 388, 461, 771, 1920
PflMI CCANNNNNTGG 3 cut(s) 830, 1871, 1913
PfoI TCCNGGA 1 cut(s) 966
PinAI ACCGGT 1 cut(s) 1741
PkrI GCNGC 1 cut(s) 297
PleI GAGTC 2 cut(s) 421, 561
PmaCI CACGTG 1 cut(s) 699
PmlI CACGTG 1 cut(s) 699
PpsI GAGTC 2 cut(s) 421, 561
Ppu21I YACGTR 3 cut(s) 699, 748, 1150
PshBI ATTAAT 1 cut(s) 1181
Psp6I CCWGG 2 cut(s) 408, 966
PspCI CACGTG 1 cut(s) 699
PspEI GGTNACC 1 cut(s) 1358
PspGI CCWGG 2 cut(s) 408, 966
PspN4I GGNNCC 6 cut(s) 445, 1624, 1740, 1862, 2044, 2074
PspOMI GGGCCC 1 cut(s) 443
PspPI GGNCC 5 cut(s) 290, 443, 444, 1739, 1891
PspXI VCTCGAGB 1 cut(s) 1475
RruI TCGCGA 1 cut(s) 346
RsaI GTAC 2 cut(s) 951, 2054
RsaNI GTAC 2 cut(s) 950, 2053
RseI CAYNNNNRTG 6 cut(s) 79, 981, 1356, 1449, 1854, 1899
SaqAI TTAA 9 cut(s) 26, 51, 692, 801, 1079, 1106, 1181, 1415, 2115
SatI GCNGC 1 cut(s) 296
Sau3AI GATC 4 cut(s) 341, 582, 1846, 2001
Sau96I GGNCC 5 cut(s) 290, 443, 444, 1739, 1891
SchI GAGTC 2 cut(s) 422, 561
ScrFI CCNGG 2 cut(s) 410, 968
SduI GDGCHC 3 cut(s) 447, 1065, 1865
SfaNI GCATC 4 cut(s) 1345, 1383, 1551, 1699
SfcI CTRYAG 2 cut(s) 430, 613
Sfr274I CTCGAG 1 cut(s) 1475
SfuI TTCGAA 3 cut(s) 1085, 1759, 1836
SinI GGWCC 1 cut(s) 1739
SlaI CTCGAG 1 cut(s) 1475
SmiMI CAYNNNNRTG 6 cut(s) 79, 981, 1356, 1449, 1854, 1899
SmlI CTYRAG 5 cut(s) 1051, 1078, 1385, 1414, 1475
SmoI CTYRAG 5 cut(s) 1051, 1078, 1385, 1414, 1475
SnaBI TACGTA 2 cut(s) 748, 1150
SphI GCATGC 1 cut(s) 1637
SsiI CCGC 3 cut(s) 239, 293, 1817
SspI AATATT 4 cut(s) 161, 818, 958, 1196
SspMI CTAG 3 cut(s) 339, 1205, 2021
StyD4I CCNGG 2 cut(s) 408, 966
StyI CCWWGG 2 cut(s) 1734, 1943
TaaI ACNGT 4 cut(s) 319, 1546, 1622, 1987
TaiI ACGT 3 cut(s) 701, 750, 1152
TaqI TCGA 5 cut(s) 1085, 1398, 1476, 1759, 1836
TatI WGTACW 1 cut(s) 949
TfiI GAWTC 5 cut(s) 144, 388, 461, 771, 1920
Tru1I TTAA 9 cut(s) 26, 51, 692, 801, 1079, 1106, 1181, 1415, 2115
Tru9I TTAA 9 cut(s) 26, 51, 692, 801, 1079, 1106, 1181, 1415, 2115
TscAI CASTG 1 cut(s) 1633
TseFI GTSAC 2 cut(s) 1349, 1358
TseI GCWGC 1 cut(s) 295
Tsp45I GTSAC 2 cut(s) 1349, 1358
TspDTI ATGAA 6 cut(s) 17, 69, 611, 951, 1018, 1296
TspGWI ACGGA 5 cut(s) 500, 595, 1088, 1308, 1742
TspRI CASTG 1 cut(s) 1633
Van91I CCANNNNNTGG 3 cut(s) 830, 1871, 1913
Vha464I CTTAAG 2 cut(s) 1078, 1414
VpaK11BI GGWCC 1 cut(s) 1739
VspI ATTAAT 1 cut(s) 1181
XapI RAATTY 4 cut(s) 88, 381, 1044, 1403
XbaI TCTAGA 1 cut(s) 338
XceI RCATGY 4 cut(s) 980, 984, 1637, 1754
XcmI CCANNNNNNNNNTGG 1 cut(s) 1528
XhoI CTCGAG 1 cut(s) 1475
XmiI GTMKAC 1 cut(s) 565
XspI CTAG 3 cut(s) 339, 1205, 2021
Zsp2I ATGCAT 1 cut(s) 982
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.