pycom04g04100

AAA domain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
N/A
Physical Location & Seq
Reverse (-)
3668025 .. 3668628
604 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 498 bp
ATGGTAGGAACATATGTGCCACAGGTCGGAGATCTCATTGCCTTGACTGATACTAAACCAAAATGCGTTGATCGTTTCAACAGGCCCAGAAATTCGTATCTTGTTGCGTATGTTCATCGGAGTAGAGGCAATAATCTCTCAATACTCTCGTCAAAGCTTATCGATAGAGTTGGATACGTGCAAATTACGAGCAAGAGAGATACGCTCTGTGCGTTCTATCTTGTGAACATGACAACAACTGTTCGCGGTAGGAATTCTTGTTCAATTTACTTTGACAACGAAAAATGCTACCTGTCTGGTACAGTTCCGGCAATGTGCTCCGATCTAAATGATTCCCAAGAAGCTGCAGTTTCAAACTGTATCAGTTTAAGTAAATTGATCGGTATGTCACTCTTTGACCCAGTTAAATTCAAGCGCAGAACATTAACACGCGCTCCAACCGGGTTAGAAGTAACAGCACGCGTGCTGAGATTATGGCAAGCACGGGATTGGAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
AAA_11 AAA_12

Protein Analysis

166

Amino Acids

18.65

Weight (kDa)

9.69

Isoelectric Point (pI)

33.81

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 3 cut(s) 246, 432, 462
AciI CCGC 1 cut(s) 246
AcsI RAATTY 3 cut(s) 91, 253, 407
AfaI GTAC 1 cut(s) 301
AfiI CCNNNNNNNGG 1 cut(s) 26
AflIII ACRYGT 1 cut(s) 460
AgsI TTSAA 4 cut(s) 79, 264, 354, 412
AloI GAACNNNNNNTCC 2 cut(s) 244, 276
AluBI AGCT 2 cut(s) 157, 344
AluI AGCT 2 cut(s) 157, 344
Alw21I GWGCWC 1 cut(s) 320
AoxI GGCC 1 cut(s) 83
ApeKI GCWGC 1 cut(s) 344
ApoI RAATTY 3 cut(s) 91, 253, 407
AspLEI GCGC 2 cut(s) 417, 434
AspS9I GGNCC 1 cut(s) 84
AsuC2I CCSGG 1 cut(s) 442
Bbv12I GWGCWC 1 cut(s) 320
BbvI GCAGC 1 cut(s) 331
BciVI GTATCC 1 cut(s) 167
BcnI CCSGG 1 cut(s) 442
BfmI CTRYAG 1 cut(s) 345
BfuI GTATCC 1 cut(s) 167
BglII AGATCT 1 cut(s) 31
BisI GCNGC 1 cut(s) 345
BlsI GCNGC 1 cut(s) 346
Bme1390I CCNGG 1 cut(s) 442
BmgT120I GGNCC 1 cut(s) 84
BmrFI CCNGG 1 cut(s) 442
BmrI ACTGGG 1 cut(s) 395
BmuI ACTGGG 1 cut(s) 395
BplI GAGNNNNNCTC 2 cut(s) 189, 221
BpuMI CCSGG 1 cut(s) 442
Bsa29I ATCGAT 1 cut(s) 162
BsaAI YACGTR 1 cut(s) 178
BsaXI ACNNNNNCTCC 2 cut(s) 418, 448
Bsc4I CCNNNNNNNGG 1 cut(s) 26
Bse1I ACTGG 1 cut(s) 401
Bse3DI GCAATG 2 cut(s) 36, 318
BseCI ATCGAT 1 cut(s) 162
BseLI CCNNNNNNNGG 1 cut(s) 26
BseMI GCAATG 2 cut(s) 36, 318
BseMII CTCAG 1 cut(s) 458
BseNI ACTGG 1 cut(s) 401
BseXI GCAGC 1 cut(s) 331
Bsh1236I CGCG 3 cut(s) 246, 432, 462
BshFI GGCC 1 cut(s) 85
BshVI ATCGAT 1 cut(s) 162
BsiHKAI GWGCWC 1 cut(s) 320
BsiSI CCGG 2 cut(s) 308, 441
BslI CCNNNNNNNGG 1 cut(s) 26
BsnI GGCC 1 cut(s) 85
Bsp1286I GDGCHC 1 cut(s) 320
Bsp143I GATC 4 cut(s) 31, 70, 322, 378
BspACI CCGC 1 cut(s) 246
BspANI GGCC 1 cut(s) 85
BspCNI CTCAG 1 cut(s) 459
BspDI ATCGAT 1 cut(s) 162
BspFNI CGCG 3 cut(s) 246, 432, 462
BspMAI CTGCAG 1 cut(s) 349
BsrDI GCAATG 2 cut(s) 36, 318
BsrI ACTGG 1 cut(s) 401
BssMI GATC 4 cut(s) 31, 70, 322, 378
Bst4CI ACNGT 3 cut(s) 241, 304, 359
BstBAI YACGTR 1 cut(s) 178
BstC8I GCNNGC 3 cut(s) 460, 464, 480
BstDEI CTNAG 1 cut(s) 467
BstFNI CGCG 3 cut(s) 246, 432, 462
BstHHI GCGC 2 cut(s) 417, 434
BstKTI GATC 4 cut(s) 34, 73, 325, 381
BstMBI GATC 4 cut(s) 31, 70, 322, 378
BstSCI CCNGG 1 cut(s) 440
BstSFI CTRYAG 1 cut(s) 345
BstUI CGCG 3 cut(s) 246, 432, 462
BstV1I GCAGC 1 cut(s) 331
BstX2I RGATCY 1 cut(s) 31
BstYI RGATCY 1 cut(s) 31
Bsu15I ATCGAT 1 cut(s) 162
BsuI GTATCC 1 cut(s) 167
BsuRI GGCC 1 cut(s) 85
BsuTUI ATCGAT 1 cut(s) 162
Cac8I GCNNGC 3 cut(s) 460, 464, 480
CfoI GCGC 2 cut(s) 417, 434
Cfr13I GGNCC 1 cut(s) 84
ClaI ATCGAT 1 cut(s) 162
Csp6I GTAC 1 cut(s) 300
CviAII CATG 1 cut(s) 229
CviJI RGCY 3 cut(s) 85, 157, 344
CviKI_1 RGCY 3 cut(s) 85, 157, 344
CviQI GTAC 1 cut(s) 300
DdeI CTNAG 1 cut(s) 467
DpnI GATC 4 cut(s) 33, 72, 324, 380
DpnII GATC 4 cut(s) 31, 70, 322, 378
EcoRI GAATTC 1 cut(s) 253
FaeI CATG 1 cut(s) 232
FaiI YATR 6 cut(s) 13, 15, 111, 230, 386, 475
FatI CATG 1 cut(s) 228
FauNDI CATATG 1 cut(s) 13
Fnu4HI GCNGC 1 cut(s) 345
Fsp4HI GCNGC 1 cut(s) 345
GlaI GCGC 2 cut(s) 416, 433
GluI GCNGC 1 cut(s) 345
HaeIII GGCC 1 cut(s) 85
HapII CCGG 2 cut(s) 308, 441
HhaI GCGC 2 cut(s) 417, 434
Hin1II CATG 1 cut(s) 232
Hin6I GCGC 2 cut(s) 415, 432
HinP1I GCGC 2 cut(s) 415, 432
HindIII AAGCTT 1 cut(s) 155
HinfI GANTC 1 cut(s) 332
HpaII CCGG 2 cut(s) 308, 441
Hpy166II GTNNAC 1 cut(s) 226
Hpy188I TCNGA 3 cut(s) 29, 120, 322
Hpy8I GTNNAC 1 cut(s) 226
HpyCH4III ACNGT 3 cut(s) 241, 304, 359
HpyCH4IV ACGT 1 cut(s) 177
HpyCH4V TGCA 2 cut(s) 181, 347
HpyF3I CTNAG 1 cut(s) 467
HpySE526I ACGT 1 cut(s) 177
Hsp92II CATG 1 cut(s) 232
HspAI GCGC 2 cut(s) 415, 432
Kzo9I GATC 4 cut(s) 31, 70, 322, 378
LmnI GCTCC 2 cut(s) 323, 439
LpnPI CCDG 8 cut(s) 8, 67, 100, 282, 305, 321, 414, 454
Lsp1109I GCAGC 1 cut(s) 331
MaeII ACGT 1 cut(s) 177
MaeIII GTNAC 2 cut(s) 387, 451
MalI GATC 4 cut(s) 33, 72, 324, 380
MboI GATC 4 cut(s) 31, 70, 322, 378
MflI RGATCY 1 cut(s) 31
MhlI GDGCHC 1 cut(s) 320
MluCI AATT 6 cut(s) 91, 183, 253, 264, 374, 407
MluI ACGCGT 1 cut(s) 460
MmeI TCCRAC 3 cut(s) 7, 151, 461
MnlI CCTC 1 cut(s) 119
MseI TTAA 3 cut(s) 368, 405, 425
MspI CCGG 2 cut(s) 308, 441
MspR9I CCNGG 1 cut(s) 442
MvnI CGCG 3 cut(s) 246, 432, 462
NciI CCSGG 1 cut(s) 442
NdeI CATATG 1 cut(s) 13
NdeII GATC 4 cut(s) 31, 70, 322, 378
NlaIII CATG 1 cut(s) 232
NmuCI GTSAC 1 cut(s) 387
PcsI WCGNNNNNNNCGW 1 cut(s) 209
PfeI GAWTC 1 cut(s) 332
PkrI GCNGC 1 cut(s) 346
Ppu21I YACGTR 1 cut(s) 178
PspPI GGNCC 1 cut(s) 84
PstI CTGCAG 1 cut(s) 349
PsuI RGATCY 1 cut(s) 31
RsaI GTAC 1 cut(s) 301
RsaNI GTAC 1 cut(s) 300
SaqAI TTAA 3 cut(s) 368, 405, 425
SatI GCNGC 1 cut(s) 345
Sau3AI GATC 4 cut(s) 31, 70, 322, 378
Sau96I GGNCC 1 cut(s) 84
ScrFI CCNGG 1 cut(s) 442
SduI GDGCHC 1 cut(s) 320
SetI ASST 5 cut(s) 27, 159, 180, 294, 346
SfcI CTRYAG 1 cut(s) 345
Sse9I AATT 6 cut(s) 91, 183, 253, 264, 374, 407
SsiI CCGC 1 cut(s) 246
StyD4I CCNGG 1 cut(s) 440
TaaI ACNGT 3 cut(s) 241, 304, 359
TaiI ACGT 1 cut(s) 180
TaqI TCGA 1 cut(s) 162
TasI AATT 6 cut(s) 91, 183, 253, 264, 374, 407
TfiI GAWTC 1 cut(s) 332
Tru1I TTAA 3 cut(s) 368, 405, 425
Tru9I TTAA 3 cut(s) 368, 405, 425
TseFI GTSAC 1 cut(s) 387
TseI GCWGC 1 cut(s) 344
Tsp45I GTSAC 1 cut(s) 387
TspDTI ATGAA 1 cut(s) 104
XapI RAATTY 3 cut(s) 91, 253, 407
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.