Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr4

Orthogroup ID

N/A

Physical Location & Seq

Genomic Coordinates Reverse (-)

6313038 .. 6314622

Gene Structure 1585 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

N/A

Sequence Viewer

CDS Protein Promoter (2k)

Length: 972 bp

ATGGTAAGGTTCTATAGGATACACCATCAGTGTCCGATACAGTCGCACTCGACCACTCCTCCTCTCGCGTTCCTTCTTTTTCTATTGCCTATATACACCACCCACACCCCTCATTCCATTTCTCACACAATCCCCAGATCCAAATCTCAAACACTCCTCAGCTCGCTCTCTCTCTCTCTGTGGTCTGTGGTTGTGGCACTGATGGGTTTCCCATCCAATGGCGCCCAGAAGCCGCTCAAGTTCCTGATCTACGGCCGGACGGGCTGGATAGGCGGCCTCCTCGGCAAGCTCTGCGAGGCCCAATCAATCGACTACGTCTACGGCTCCGGCCGCCTCGAGAATCGCAGCTCGCTCGAGGCCGACATCGCCAATATTAAGCCCACCCACGTCTTCAACGCCGCCGGCGTCACCGGCCGCCCCAACGTCGACTGGTGCGAATCCCATAAGGTCGAGACCATCCGGACCAACGTGGTCGGCACCCTGACCCTCGCTGACGTATGCAGAGAGCGGGGGTTGGTCCTCATTAACTATGCGACCGGGTGCATTTTCGAGTACGATGCGGGTCACCCGCTCGGGTCCGGCGTCGGGTTCAAGGAGGAGGACACCCCCAACTTTATCGGATCTTTCTATTCCAAGACAAAGGCCATGGTGGAAGATCTGCTCAACAATTTCGAGAACGTTTGTACTTTGCGCGTTCGGATGCCGATTTCATCCGACTTATCCAACCCCCGTAACTTCATCACGAAGATCACTCGATACGAGAAGGTAGTCGACATTCCAAACTCCATGACAATCTTGGACGAACTCCTCCCCATTTCCATCGAGATGGCAAAGAGAAACCTCACCGGAATCTACAACTTCACAAACCCCGGAGTGGTCAGCCACAACGATCGATCTTGGAGATGTACAAGGAATACATTGACCCCAGCTTCACTTACAAGAACTTTACTCTTGAGGAGCAGGCAAAGGTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0005911 GO:0006139 GO:0006725 GO:0006793 GO:0006807 GO:0006810 GO:0008150 GO:0008152 GO:0009058 GO:0009225 GO:0009226 GO:0009506 GO:0009812 GO:0009813 GO:0009914 GO:0009966 GO:0009987 GO:0010253 GO:0010280 GO:0010315 GO:0010646 GO:0010817 GO:0010928 GO:0016829 GO:0016835 GO:0016836 GO:0018130 GO:0019438 GO:0023051 GO:0030054 GO:0030154 GO:0032502 GO:0033478 GO:0034641 GO:0034654 GO:0042127 GO:0042440 GO:0044237 GO:0044238 GO:0044249 GO:0044271 GO:0044281 GO:0044424 GO:0044444 GO:0044464 GO:0046148 GO:0046483 GO:0048583 GO:0048869 GO:0050377 GO:0050789 GO:0050794 GO:0051179 GO:0051234 GO:0051552 GO:0051553 GO:0051554 GO:0051555 GO:0055044 GO:0055086 GO:0060918 GO:0065007 GO:0065008 GO:0071704 GO:1901135 GO:1901137 GO:1901360 GO:1901362 GO:1901576 GO:1901615 GO:1901617

KEGG Pathways

Metabolic & Signaling

ko00520 ko00523 ko01130 map00520 map00523 map01130

Pfam Domains

Protein Families

RmlD_sub_bind

Protein Analysis

324

Amino Acids

36.12

Weight (kDa)

9.27

Isoelectric Point (pI)

31.48

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
RmlD_sub_bind	PF04321	80 - 252	6e-14	RmlD substrate binding domain
Epimerase	PF01370	82 - 244	4.8e-12	NAD dependent epimerase/dehydratase family

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB1I	GGYRCC	2 cut(s)	221, 476
AccB7I	CCANNNNNTGG	1 cut(s)	218
AccBSI	CCGCTC	3 cut(s)	235, 508, 571
AccI	GTMKAC	3 cut(s)	318, 426, 771
AccII	CGCG	2 cut(s)	68, 693
AccIII	TCCGGA	1 cut(s)	459
AciI	CCGC	8 cut(s)	233, 273, 331, 399, 415, 508, 560, 569
AclI	AACGTT	1 cut(s)	678
AclWI	GGATC	2 cut(s)	132, 628
AcoI	YGGCCR	3 cut(s)	253, 328, 412
AcyI	GRCGYC	3 cut(s)	222, 405, 582
AfaI	GTAC	3 cut(s)	554, 685, 907
AfiI	CCNNNNNNNGG	3 cut(s)	218, 585, 874
AgsI	TTSAA	2 cut(s)	394, 592
AjiI	CACGTC	1 cut(s)	388
AluBI	AGCT	4 cut(s)	162, 289, 348, 929
AluI	AGCT	4 cut(s)	162, 289, 348, 929
Alw26I	GTCTC	1 cut(s)	446
AlwI	GGATC	2 cut(s)	132, 628
Ama87I	CYCGRG	3 cut(s)	335, 353, 572
Aor13HI	TCCGGA	1 cut(s)	459
AoxI	GGCC	7 cut(s)	253, 274, 297, 328, 357, 412, 642
ApeKI	GCWGC	1 cut(s)	345
AspLEI	GCGC	2 cut(s)	224, 693
AspS9I	GGNCC	4 cut(s)	298, 462, 517, 576
AsuC2I	CCSGG	2 cut(s)	538, 870
AsuHPI	GGTGA	3 cut(s)	400, 557, 835
AvaI	CYCGRG	3 cut(s)	335, 353, 572
AvaII	GGWCC	3 cut(s)	462, 517, 576
BanI	GGYRCC	2 cut(s)	221, 476
BbsI	GAAGAC	1 cut(s)	382
BbvCI	CCTCAGC	1 cut(s)	158
BbvI	GCAGC	1 cut(s)	357
BccI	CCATC	6 cut(s)	33, 196, 220, 464, 820, 827
BceAI	ACGGC	2 cut(s)	268, 337
BcgI	CGANNNNNNTGC	4 cut(s)	334, 368, 539, 573
BciVI	GTATCC	1 cut(s)	12
BcnI	CCSGG	2 cut(s)	538, 870
BcoDI	GTCTC	1 cut(s)	446
BfmI	CTRYAG	1 cut(s)	13
BfoI	RGCGCY	1 cut(s)	225
BfuI	GTATCC	1 cut(s)	12
BglI	GCCNNNNNGGC	2 cut(s)	261, 282
BglII	AGATCT	1 cut(s)	655
BisI	GCNGC	6 cut(s)	233, 274, 331, 346, 399, 415
BlsI	GCNGC	6 cut(s)	234, 275, 332, 347, 400, 416
Bme1390I	CCNGG	2 cut(s)	538, 870
Bme18I	GGWCC	3 cut(s)	462, 517, 576
BmeT110I	CYCGRG	3 cut(s)	335, 353, 572
BmgBI	CACGTC	1 cut(s)	388
BmgT120I	GGNCC	4 cut(s)	298, 462, 517, 576
BmiI	GGNNCC	4 cut(s)	223, 325, 478, 577
BmrFI	CCNGG	2 cut(s)	538, 870
BmsI	GCATC	2 cut(s)	547, 690
BpiI	GAAGAC	1 cut(s)	382
Bpu10I	CCTNAGC	1 cut(s)	158
BpuEI	CTTGAG	1 cut(s)	221
BpuMI	CCSGG	2 cut(s)	538, 870
Bsa29I	ATCGAT	1 cut(s)	892
BsaBI	GATNNNNATC	1 cut(s)	142
BsaHI	GRCGYC	3 cut(s)	222, 405, 582
BsaI	GGTCTC	1 cut(s)	446
BsaJI	CCNNGG	3 cut(s)	280, 645, 868
BsaWI	WCCGGW	2 cut(s)	459, 845
BsaXI	ACNNNNNCTCC	2 cut(s)	43, 73
Bsc4I	CCNNNNNNNGG	3 cut(s)	218, 585, 874
Bse118I	RCCGGY	2 cut(s)	401, 410
Bse1I	ACTGG	1 cut(s)	434
Bse8I	GATNNNNATC	1 cut(s)	142
BseAI	TCCGGA	1 cut(s)	459
BseCI	ATCGAT	1 cut(s)	892
BseDI	CCNNGG	3 cut(s)	280, 645, 868
BseGI	GGATG	4 cut(s)	212, 456, 705, 710
BseJI	GATNNNNATC	1 cut(s)	142
BseLI	CCNNNNNNNGG	3 cut(s)	218, 585, 874
BseMII	CTCAG	1 cut(s)	172
BseNI	ACTGG	1 cut(s)	434
BseRI	GAGGAG	7 cut(s)	48, 51, 146, 269, 611, 797, 970
BseX3I	CGGCCG	3 cut(s)	253, 328, 412
BseXI	GCAGC	1 cut(s)	357
BseYI	CCCAGC	1 cut(s)	925
Bsh1236I	CGCG	2 cut(s)	68, 693
Bsh1285I	CGRYCG	5 cut(s)	256, 331, 415, 537, 892
BshFI	GGCC	7 cut(s)	255, 276, 299, 330, 359, 414, 644
BshNI	GGYRCC	2 cut(s)	221, 476
BshVI	ATCGAT	1 cut(s)	892
BsiEI	CGRYCG	5 cut(s)	256, 331, 415, 537, 892
BsiHKCI	CYCGRG	3 cut(s)	335, 353, 572
BsiSI	CCGG	9 cut(s)	256, 327, 402, 411, 460, 537, 579, 846, 870
BslI	CCNNNNNNNGG	3 cut(s)	218, 585, 874
BsmAI	GTCTC	1 cut(s)	446
BsnI	GGCC	7 cut(s)	255, 276, 299, 330, 359, 414, 644
Bso31I	GGTCTC	1 cut(s)	446
BsoBI	CYCGRG	3 cut(s)	335, 353, 572
Bsp13I	TCCGGA	1 cut(s)	459
Bsp1407I	TGTACA	1 cut(s)	905
Bsp143I	GATC	7 cut(s)	137, 246, 620, 655, 747, 889, 893
Bsp19I	CCATGG	1 cut(s)	645
BspACI	CCGC	8 cut(s)	233, 273, 331, 399, 415, 508, 560, 569
BspANI	GGCC	7 cut(s)	255, 276, 299, 330, 359, 414, 644
BspCNI	CTCAG	1 cut(s)	171
BspDI	ATCGAT	1 cut(s)	892
BspEI	TCCGGA	1 cut(s)	459
BspFNI	CGCG	2 cut(s)	68, 693
BspLI	GGNNCC	4 cut(s)	223, 325, 478, 577
BspPI	GGATC	2 cut(s)	132, 628
BspT107I	GGYRCC	2 cut(s)	221, 476
BspTNI	GGTCTC	1 cut(s)	446
BsrBI	CCGCTC	3 cut(s)	235, 508, 571
BsrFI	RCCGGY	2 cut(s)	401, 410
BsrGI	TGTACA	1 cut(s)	905
BsrI	ACTGG	1 cut(s)	434
BssAI	RCCGGY	2 cut(s)	401, 410
BssECI	CCNNGG	3 cut(s)	280, 645, 868
BssMI	GATC	7 cut(s)	137, 246, 620, 655, 747, 889, 893
BssNI	GRCGYC	3 cut(s)	222, 405, 582
BssT1I	CCWWGG	1 cut(s)	645
Bst4CI	ACNGT	1 cut(s)	42
BstACI	GRCGYC	3 cut(s)	222, 405, 582
BstAPI	GCANNNNNTGC	1 cut(s)	291
BstAUI	TGTACA	1 cut(s)	905
BstC8I	GCNNGC	5 cut(s)	164, 287, 350, 403, 962
BstDEI	CTNAG	1 cut(s)	158
BstDSI	CCRYGG	1 cut(s)	645
BstEII	GGTNACC	1 cut(s)	563
BstF5I	GGATG	4 cut(s)	212, 456, 705, 710
BstFNI	CGCG	2 cut(s)	68, 693
BstH2I	RGCGCY	1 cut(s)	225
BstHHI	GCGC	2 cut(s)	224, 693
BstKTI	GATC	7 cut(s)	140, 249, 623, 658, 750, 892, 896
BstMAI	GTCTC	1 cut(s)	446
BstMBI	GATC	7 cut(s)	137, 246, 620, 655, 747, 889, 893
BstMCI	CGRYCG	5 cut(s)	256, 331, 415, 537, 892
BstMWI	GCNNNNNNNGC	7 cut(s)	261, 270, 282, 291, 330, 365, 411
BstPI	GGTNACC	1 cut(s)	563
BstSCI	CCNGG	2 cut(s)	536, 868
BstSFI	CTRYAG	1 cut(s)	13
BstUI	CGCG	2 cut(s)	68, 693
BstV1I	GCAGC	1 cut(s)	357
BstV2I	GAAGAC	1 cut(s)	382
BstX2I	RGATCY	3 cut(s)	137, 620, 655
BstXI	CCANNNNNNTGG	1 cut(s)	826
BstYI	RGATCY	3 cut(s)	137, 620, 655
BstZI	CGGCCG	3 cut(s)	253, 328, 412
Bsu15I	ATCGAT	1 cut(s)	892
BsuI	GTATCC	1 cut(s)	12
BsuRI	GGCC	7 cut(s)	255, 276, 299, 330, 359, 414, 644
BsuTUI	ATCGAT	1 cut(s)	892
BtgI	CCRYGG	1 cut(s)	645
BtgZI	GCGATG	1 cut(s)	349
BtrI	CACGTC	1 cut(s)	388
BtsCI	GGATG	4 cut(s)	212, 456, 705, 710
BtsIMutI	CAGTG	2 cut(s)	35, 197
Cac8I	GCNNGC	5 cut(s)	164, 287, 350, 403, 962
CfoI	GCGC	2 cut(s)	224, 693
Cfr10I	RCCGGY	2 cut(s)	401, 410
Cfr13I	GGNCC	4 cut(s)	298, 462, 517, 576
ClaI	ATCGAT	1 cut(s)	892
CseI	GACGC	2 cut(s)	394, 571
Csp6I	GTAC	3 cut(s)	553, 684, 906
CviAII	CATG	2 cut(s)	646, 787
CviQI	GTAC	3 cut(s)	553, 684, 906
DdeI	CTNAG	1 cut(s)	158
DinI	GGCGCC	1 cut(s)	223
DpnI	GATC	7 cut(s)	139, 248, 622, 657, 749, 891, 895
DpnII	GATC	7 cut(s)	137, 246, 620, 655, 747, 889, 893
EaeI	YGGCCR	3 cut(s)	253, 328, 412
EagI	CGGCCG	3 cut(s)	253, 328, 412
EclXI	CGGCCG	3 cut(s)	253, 328, 412
Eco130I	CCWWGG	1 cut(s)	645
Eco31I	GGTCTC	1 cut(s)	446
Eco47I	GGWCC	3 cut(s)	462, 517, 576
Eco52I	CGGCCG	3 cut(s)	253, 328, 412
Eco88I	CYCGRG	3 cut(s)	335, 353, 572
Eco91I	GGTNACC	1 cut(s)	563
EcoO65I	GGTNACC	1 cut(s)	563
EcoT14I	CCWWGG	1 cut(s)	645
EgeI	GGCGCC	1 cut(s)	223
EheI	GGCGCC	1 cut(s)	223
ErhI	CCWWGG	1 cut(s)	645
FaeI	CATG	2 cut(s)	649, 790
FaiI	YATR	8 cut(s)	15, 92, 94, 444, 499, 531, 647, 788
FatI	CATG	2 cut(s)	645, 786
FauI	CCCGC	3 cut(s)	501, 553, 576
FblI	GTMKAC	3 cut(s)	318, 426, 771
Fnu4HI	GCNGC	6 cut(s)	233, 274, 331, 346, 399, 415
FokI	GGATG	4 cut(s)	199, 443, 697, 712
Fsp4HI	GCNGC	6 cut(s)	233, 274, 331, 346, 399, 415
GlaI	GCGC	2 cut(s)	223, 692
GluI	GCNGC	6 cut(s)	233, 274, 331, 346, 399, 415
GsaI	CCCAGC	1 cut(s)	929
HaeII	RGCGCY	1 cut(s)	225
HaeIII	GGCC	7 cut(s)	255, 276, 299, 330, 359, 414, 644
HapII	CCGG	9 cut(s)	256, 327, 402, 411, 460, 537, 579, 846, 870
HgaI	GACGC	2 cut(s)	394, 571
HhaI	GCGC	2 cut(s)	224, 693
Hin1I	GRCGYC	3 cut(s)	222, 405, 582
Hin1II	CATG	2 cut(s)	649, 790
Hin6I	GCGC	2 cut(s)	222, 691
HinP1I	GCGC	2 cut(s)	222, 691
HincII	GTYRAC	2 cut(s)	427, 772
HindII	GTYRAC	2 cut(s)	427, 772
HinfI	GANTC	3 cut(s)	340, 437, 849
HpaII	CCGG	9 cut(s)	256, 327, 402, 411, 460, 537, 579, 846, 870
HphI	GGTGA	3 cut(s)	400, 557, 835
Hpy166II	GTNNAC	3 cut(s)	319, 427, 772
Hpy188I	TCNGA	4 cut(s)	36, 620, 699, 715
Hpy188III	TCNNGA	8 cut(s)	244, 337, 451, 460, 673, 742, 823, 952
Hpy8I	GTNNAC	3 cut(s)	319, 427, 772
Hpy99I	CGWCG	2 cut(s)	428, 587
HpyAV	CCTTC	2 cut(s)	83, 757
HpyCH4III	ACNGT	1 cut(s)	42
HpyCH4IV	ACGT	6 cut(s)	315, 387, 423, 468, 495, 678
HpyCH4V	TGCA	2 cut(s)	501, 543
HpyF10VI	GCNNNNNNNGC	7 cut(s)	261, 270, 282, 291, 330, 365, 411
HpyF3I	CTNAG	1 cut(s)	158
HpySE526I	ACGT	6 cut(s)	315, 387, 423, 468, 495, 678
Hsp92I	GRCGYC	3 cut(s)	222, 405, 582
Hsp92II	CATG	2 cut(s)	649, 790
HspAI	GCGC	2 cut(s)	222, 691
KasI	GGCGCC	1 cut(s)	221
Kpn2I	TCCGGA	1 cut(s)	459
KroI	GCCGGC	1 cut(s)	401
KroNI	GCCGGC	1 cut(s)	403
Kzo9I	GATC	7 cut(s)	137, 246, 620, 655, 747, 889, 893
LmnI	GCTCC	2 cut(s)	329, 957
Lsp1109I	GCAGC	1 cut(s)	357
LweI	GCATC	2 cut(s)	547, 690
MaeII	ACGT	6 cut(s)	315, 387, 423, 468, 495, 678
MaeIII	GTNAC	3 cut(s)	406, 563, 731
MalI	GATC	7 cut(s)	139, 248, 622, 657, 749, 891, 895
MbiI	CCGCTC	3 cut(s)	235, 508, 571
MboI	GATC	7 cut(s)	137, 246, 620, 655, 747, 889, 893
MboII	GAAGA	3 cut(s)	382, 665, 757
MflI	RGATCY	3 cut(s)	137, 620, 655
MluCI	AATT	1 cut(s)	667
Mly113I	GGCGCC	1 cut(s)	222
MmeI	TCCRAC	2 cut(s)	738, 747
MreI	CGCCGGCG	1 cut(s)	401
MroI	TCCGGA	1 cut(s)	459
MroNI	GCCGGC	1 cut(s)	401
MseI	TTAA	2 cut(s)	375, 525
MslI	CAYNNNNRTG	1 cut(s)	824
MspI	CCGG	9 cut(s)	256, 327, 402, 411, 460, 537, 579, 846, 870
MspR9I	CCNGG	2 cut(s)	538, 870
MvnI	CGCG	2 cut(s)	68, 693
MwoI	GCNNNNNNNGC	7 cut(s)	261, 270, 282, 291, 330, 365, 411
NaeI	GCCGGC	1 cut(s)	403
NarI	GGCGCC	1 cut(s)	222
NciI	CCSGG	2 cut(s)	538, 870
NcoI	CCATGG	1 cut(s)	645
NdeII	GATC	7 cut(s)	137, 246, 620, 655, 747, 889, 893
NgoMIV	GCCGGC	1 cut(s)	401
NlaIII	CATG	2 cut(s)	649, 790
NlaIV	GGNNCC	4 cut(s)	223, 325, 478, 577
NmeAIII	GCCGAG	1 cut(s)	261
NmuCI	GTSAC	2 cut(s)	406, 563
PaeR7I	CTCGAG	2 cut(s)	335, 353
PcsI	WCGNNNNNNNCGW	3 cut(s)	402, 432, 579
PdiI	GCCGGC	1 cut(s)	403
PfeI	GAWTC	3 cut(s)	340, 437, 849
PflFI	GACNNNGTC	1 cut(s)	314
PflMI	CCANNNNNTGG	1 cut(s)	218
PkrI	GCNGC	6 cut(s)	234, 275, 332, 347, 400, 416
Ple19I	CGATCG	1 cut(s)	892
PluTI	GGCGCC	1 cut(s)	225
Psp1406I	AACGTT	1 cut(s)	678
PspEI	GGTNACC	1 cut(s)	563
PspFI	CCCAGC	1 cut(s)	925
PspN4I	GGNNCC	4 cut(s)	223, 325, 478, 577
PspPI	GGNCC	4 cut(s)	298, 462, 517, 576
PspXI	VCTCGAGB	1 cut(s)	353
PsuI	RGATCY	3 cut(s)	137, 620, 655
PsyI	GACNNNGTC	1 cut(s)	314
PvuI	CGATCG	1 cut(s)	892
RsaI	GTAC	3 cut(s)	554, 685, 907
RsaNI	GTAC	3 cut(s)	553, 684, 906
RseI	CAYNNNNRTG	1 cut(s)	824
SalI	GTCGAC	2 cut(s)	425, 770
SaqAI	TTAA	2 cut(s)	375, 525
SatI	GCNGC	6 cut(s)	233, 274, 331, 346, 399, 415
Sau3AI	GATC	7 cut(s)	137, 246, 620, 655, 747, 889, 893
Sau96I	GGNCC	4 cut(s)	298, 462, 517, 576
ScrFI	CCNGG	2 cut(s)	538, 870
SfaNI	GCATC	2 cut(s)	547, 690
SfcI	CTRYAG	1 cut(s)	13
SfoI	GGCGCC	1 cut(s)	223
Sfr274I	CTCGAG	2 cut(s)	335, 353
SgrAI	CRCCGGYG	1 cut(s)	401
SinI	GGWCC	3 cut(s)	462, 517, 576
SlaI	CTCGAG	2 cut(s)	335, 353
SmiMI	CAYNNNNRTG	1 cut(s)	824
SmlI	CTYRAG	4 cut(s)	236, 335, 353, 952
SmoI	CTYRAG	4 cut(s)	236, 335, 353, 952
Sse9I	AATT	1 cut(s)	667
SsiI	CCGC	8 cut(s)	233, 273, 331, 399, 415, 508, 560, 569
SspDI	GGCGCC	1 cut(s)	221
SspI	AATATT	1 cut(s)	373
StyD4I	CCNGG	2 cut(s)	536, 868
StyI	CCWWGG	1 cut(s)	645
TaaI	ACNGT	1 cut(s)	42
TaiI	ACGT	6 cut(s)	318, 390, 426, 471, 498, 681
TasI	AATT	1 cut(s)	667
TatI	WGTACW	2 cut(s)	683, 905
TauI	GCSGC	5 cut(s)	235, 276, 333, 401, 417
TfiI	GAWTC	3 cut(s)	340, 437, 849
Tru1I	TTAA	2 cut(s)	375, 525
Tru9I	TTAA	2 cut(s)	375, 525
TscAI	CASTG	2 cut(s)	35, 204
TseFI	GTSAC	2 cut(s)	406, 563
TseI	GCWGC	1 cut(s)	345
Tsp45I	GTSAC	2 cut(s)	406, 563
TspDTI	ATGAA	2 cut(s)	699, 727
TspRI	CASTG	2 cut(s)	35, 204
Tth111I	GACNNNGTC	1 cut(s)	314
Van91I	CCANNNNNTGG	1 cut(s)	218
VpaK11BI	GGWCC	3 cut(s)	462, 517, 576
XcmI	CCANNNNNNNNNTGG	1 cut(s)	793
XhoI	CTCGAG	2 cut(s)	335, 353
XmiI	GTMKAC	3 cut(s)	318, 426, 771

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.