pycom04g07190

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
N/A
Physical Location & Seq
Reverse (-)
7562809 .. 7563450
642 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 642 bp
ATGCAAAACGCACCGTTACATCCCTCACTTGGCCACCATTCTGGCAATGAAGTCCTCGTATTTGATCTGCCATCGGACCCGACATCCACCTCCCGGATCCACTCGTCGAACTCGGACGGCTCCAATTTCTCGCCGATGCTGGTGAGGATGTGCCGGAGCTCCGATACGGAGACGAAGCCGGTGGAGTCCTTGTCGAGGACCTTGAAGGCGTCGCGGAGCTGGCGATCGAAAGGCTCGGGCTTCATGTGCTTGGCCATGAGGTCGAGAAAGCGAGGGAAGTCGAAGGGGGCGGTGAGCTTCTCCTCGGCGACTATGGATTTGAGCTGGGCCTGGGTCGGGTTGCCGCCGAGCGATCGCATTAGGATCCCGAGCTCCGACGGAGCAATCTTGCCGTCGTTGTCGGTGTCGAATAGAGTGAACGCCTCCTTCATCGACGATACTTGGTCATCGCTCAGATCCTTGCCCATTTTTTCCGGGTTCGCGATTCGGGTTTTCGGGTCTCTCTCTCTCTCTCTCTCTCTGCGAAACTGGCTGGCTTATTTCCCTTTAATTTCTCTCGGAGATATGACAAGGGTGGAAAGGAATTTGGGATTTGAACAAAGGGAAGGAAATTGTGCGAGCGCTAAAGCGGCGTCGTTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

214

Amino Acids

23.24

Weight (kDa)

10.2

Isoelectric Point (pI)

42.27

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 214, 482
AciI CCGC 4 cut(s) 214, 290, 344, 629
AclWI GGATC 5 cut(s) 91, 104, 358, 371, 450
AcoI YGGCCR 2 cut(s) 31, 252
AcsI RAATTY 1 cut(s) 583
AcyI GRCGYC 2 cut(s) 209, 632
AfeI AGCGCT 1 cut(s) 622
AfiI CCNNNNNNNGG 4 cut(s) 29, 93, 195, 336
AgsI TTSAA 2 cut(s) 205, 596
AjnI CCWGG 1 cut(s) 329
AluBI AGCT 5 cut(s) 159, 219, 297, 324, 372
AluI AGCT 5 cut(s) 159, 219, 297, 324, 372
Alw21I GWGCWC 2 cut(s) 161, 374
Alw26I GTCTC 2 cut(s) 164, 504
AlwI GGATC 5 cut(s) 91, 104, 358, 371, 450
Ama87I CYCGRG 2 cut(s) 235, 367
Aor51HI AGCGCT 1 cut(s) 622
AoxI GGCC 3 cut(s) 31, 252, 327
ApoI RAATTY 1 cut(s) 583
ArsI GACNNNNNNTTYG 2 cut(s) 301, 333
AsiSI GCGATCGC 1 cut(s) 355
AspLEI GCGC 1 cut(s) 623
AspS9I GGNCC 3 cut(s) 76, 198, 327
AsuC2I CCSGG 2 cut(s) 94, 475
AsuHPI GGTGA 2 cut(s) 154, 304
AvaI CYCGRG 2 cut(s) 235, 367
AvaII GGWCC 2 cut(s) 76, 198
BalI TGGCCA 2 cut(s) 33, 254
BamHI GGATCC 2 cut(s) 96, 363
BanII GRGCYC 2 cut(s) 161, 374
Bbv12I GWGCWC 2 cut(s) 161, 374
BccI CCATC 1 cut(s) 79
BceAI ACGGC 2 cut(s) 133, 376
BciT130I CCWGG 1 cut(s) 331
BcnI CCSGG 2 cut(s) 94, 475
BcoDI GTCTC 2 cut(s) 164, 504
BfoI RGCGCY 1 cut(s) 624
BisI GCNGC 2 cut(s) 344, 630
BlsI GCNGC 2 cut(s) 345, 631
Bme1390I CCNGG 3 cut(s) 94, 331, 475
Bme18I GGWCC 2 cut(s) 76, 198
BmeT110I CYCGRG 2 cut(s) 235, 367
BmgT120I GGNCC 3 cut(s) 76, 198, 327
BmiI GGNNCC 4 cut(s) 78, 98, 121, 365
BmrFI CCNGG 3 cut(s) 94, 331, 475
BmsI GCATC 1 cut(s) 126
BpuMI CCSGG 2 cut(s) 94, 475
BsaHI GRCGYC 2 cut(s) 209, 632
BsaI GGTCTC 1 cut(s) 504
BsaJI CCNNGG 2 cut(s) 303, 330
BsaXI ACNNNNNCTCC 2 cut(s) 176, 206
Bsc4I CCNNNNNNNGG 4 cut(s) 29, 93, 195, 336
Bse118I RCCGGY 1 cut(s) 178
Bse1I ACTGG 1 cut(s) 533
Bse3DI GCAATG 1 cut(s) 52
BseBI CCWGG 1 cut(s) 331
BseDI CCNNGG 2 cut(s) 303, 330
BseGI GGATG 3 cut(s) 19, 83, 153
BseLI CCNNNNNNNGG 4 cut(s) 29, 93, 195, 336
BseMI GCAATG 1 cut(s) 52
BseMII CTCAG 1 cut(s) 466
BseNI ACTGG 1 cut(s) 533
BseRI GAGGAG 1 cut(s) 292
BseYI CCCAGC 1 cut(s) 324
Bsh1236I CGCG 2 cut(s) 214, 482
Bsh1285I CGRYCG 2 cut(s) 227, 355
BshFI GGCC 3 cut(s) 33, 254, 329
BsiEI CGRYCG 2 cut(s) 227, 355
BsiHKAI GWGCWC 2 cut(s) 161, 374
BsiHKCI CYCGRG 2 cut(s) 235, 367
BsiSI CCGG 4 cut(s) 94, 154, 179, 474
BslI CCNNNNNNNGG 4 cut(s) 29, 93, 195, 336
BsmAI GTCTC 2 cut(s) 164, 504
BsmBI CGTCTC 1 cut(s) 164
BsnI GGCC 3 cut(s) 33, 254, 329
Bso31I GGTCTC 1 cut(s) 504
BsoBI CYCGRG 2 cut(s) 235, 367
Bsp1286I GDGCHC 2 cut(s) 161, 374
Bsp143I GATC 6 cut(s) 64, 96, 224, 352, 363, 455
Bsp68I TCGCGA 1 cut(s) 482
BspACI CCGC 4 cut(s) 214, 290, 344, 629
BspANI GGCC 3 cut(s) 33, 254, 329
BspCNI CTCAG 1 cut(s) 465
BspFNI CGCG 2 cut(s) 214, 482
BspLI GGNNCC 4 cut(s) 78, 98, 121, 365
BspPI GGATC 5 cut(s) 91, 104, 358, 371, 450
BspTNI GGTCTC 1 cut(s) 504
BsrDI GCAATG 1 cut(s) 52
BsrFI RCCGGY 1 cut(s) 178
BsrI ACTGG 1 cut(s) 533
BssAI RCCGGY 1 cut(s) 178
BssECI CCNNGG 2 cut(s) 303, 330
BssMI GATC 6 cut(s) 64, 96, 224, 352, 363, 455
BssNI GRCGYC 2 cut(s) 209, 632
Bst2UI CCWGG 1 cut(s) 331
Bst4CI ACNGT 1 cut(s) 15
BstACI GRCGYC 2 cut(s) 209, 632
BstC8I GCNNGC 3 cut(s) 221, 534, 619
BstDEI CTNAG 1 cut(s) 452
BstENI CCTNNNNNAGG 1 cut(s) 193
BstF5I GGATG 3 cut(s) 19, 83, 153
BstFNI CGCG 2 cut(s) 214, 482
BstH2I RGCGCY 1 cut(s) 624
BstHHI GCGC 1 cut(s) 623
BstKTI GATC 6 cut(s) 67, 99, 227, 355, 366, 458
BstMAI GTCTC 2 cut(s) 164, 504
BstMBI GATC 6 cut(s) 64, 96, 224, 352, 363, 455
BstMCI CGRYCG 2 cut(s) 227, 355
BstMWI GCNNNNNNNGC 4 cut(s) 220, 246, 529, 629
BstNI CCWGG 1 cut(s) 331
BstSCI CCNGG 3 cut(s) 92, 329, 473
BstUI CGCG 2 cut(s) 214, 482
BstX2I RGATCY 3 cut(s) 96, 363, 455
BstXI CCANNNNNNTGG 1 cut(s) 41
BstYI RGATCY 3 cut(s) 96, 363, 455
BsuRI GGCC 3 cut(s) 33, 254, 329
BtgZI GCGATG 1 cut(s) 432
BtsCI GGATG 3 cut(s) 19, 83, 153
BtuMI TCGCGA 1 cut(s) 482
Cac8I GCNNGC 3 cut(s) 221, 534, 619
CfoI GCGC 1 cut(s) 623
Cfr10I RCCGGY 1 cut(s) 178
Cfr13I GGNCC 3 cut(s) 76, 198, 327
CseI GACGC 2 cut(s) 198, 621
CviAII CATG 2 cut(s) 244, 256
DdeI CTNAG 1 cut(s) 452
DpnI GATC 6 cut(s) 66, 98, 226, 354, 365, 457
DpnII GATC 6 cut(s) 64, 96, 224, 352, 363, 455
EaeI YGGCCR 2 cut(s) 31, 252
Ecl136II GAGCTC 2 cut(s) 159, 372
Eco24I GRGCYC 2 cut(s) 161, 374
Eco31I GGTCTC 1 cut(s) 504
Eco47I GGWCC 2 cut(s) 76, 198
Eco47III AGCGCT 1 cut(s) 622
Eco53kI GAGCTC 2 cut(s) 159, 372
Eco88I CYCGRG 2 cut(s) 235, 367
EcoICRI GAGCTC 2 cut(s) 159, 372
EcoNI CCTNNNNNAGG 1 cut(s) 193
EcoO109I RGGNCCY 1 cut(s) 198
EcoRII CCWGG 1 cut(s) 329
EcoT38I GRGCYC 2 cut(s) 161, 374
Esp3I CGTCTC 1 cut(s) 164
FaeI CATG 2 cut(s) 247, 259
FaiI YATR 4 cut(s) 245, 257, 314, 566
FatI CATG 2 cut(s) 243, 255
Fnu4HI GCNGC 2 cut(s) 344, 630
FokI GGATG 3 cut(s) 6, 70, 160
FriOI GRGCYC 2 cut(s) 161, 374
Fsp4HI GCNGC 2 cut(s) 344, 630
GlaI GCGC 1 cut(s) 622
GluI GCNGC 2 cut(s) 344, 630
GsaI CCCAGC 1 cut(s) 328
HaeII RGCGCY 1 cut(s) 624
HaeIII GGCC 3 cut(s) 33, 254, 329
HapII CCGG 4 cut(s) 94, 154, 179, 474
HgaI GACGC 2 cut(s) 198, 621
HhaI GCGC 1 cut(s) 623
Hin1I GRCGYC 2 cut(s) 209, 632
Hin1II CATG 2 cut(s) 247, 259
Hin6I GCGC 1 cut(s) 621
HinP1I GCGC 1 cut(s) 621
HinfI GANTC 2 cut(s) 185, 484
HpaII CCGG 4 cut(s) 94, 154, 179, 474
HphI GGTGA 2 cut(s) 154, 304
Hpy166II GTNNAC 1 cut(s) 418
Hpy188I TCNGA 6 cut(s) 76, 115, 163, 376, 455, 560
Hpy188III TCNNGA 3 cut(s) 264, 367, 481
Hpy8I GTNNAC 1 cut(s) 418
Hpy99I CGWCG 6 cut(s) 109, 214, 380, 397, 437, 637
HpyAV CCTTC 4 cut(s) 199, 277, 436, 599
HpyCH4III ACNGT 1 cut(s) 15
HpyCH4V TGCA 1 cut(s) 4
HpyF10VI GCNNNNNNNGC 4 cut(s) 220, 246, 529, 629
HpyF3I CTNAG 1 cut(s) 452
Hsp92I GRCGYC 2 cut(s) 209, 632
Hsp92II CATG 2 cut(s) 247, 259
HspAI GCGC 1 cut(s) 621
Kzo9I GATC 6 cut(s) 64, 96, 224, 352, 363, 455
LmnI GCTCC 6 cut(s) 125, 156, 164, 216, 377, 380
LweI GCATC 1 cut(s) 126
MaeIII GTNAC 1 cut(s) 15
MalI GATC 6 cut(s) 66, 98, 226, 354, 365, 457
MboI GATC 6 cut(s) 64, 96, 224, 352, 363, 455
MflI RGATCY 3 cut(s) 96, 363, 455
MhlI GDGCHC 2 cut(s) 161, 374
MlsI TGGCCA 2 cut(s) 33, 254
MluCI AATT 4 cut(s) 124, 549, 583, 610
MluNI TGGCCA 2 cut(s) 33, 254
MlyI GAGTC 1 cut(s) 194
MmeI TCCRAC 1 cut(s) 399
MnlI CCTC 9 cut(s) 34, 65, 100, 138, 189, 252, 266, 313, 433
Mox20I TGGCCA 2 cut(s) 33, 254
MscI TGGCCA 2 cut(s) 33, 254
MseI TTAA 1 cut(s) 548
Msp20I TGGCCA 2 cut(s) 33, 254
MspI CCGG 4 cut(s) 94, 154, 179, 474
MspR9I CCNGG 3 cut(s) 94, 331, 475
MvaI CCWGG 1 cut(s) 331
MvnI CGCG 2 cut(s) 214, 482
MwoI GCNNNNNNNGC 4 cut(s) 220, 246, 529, 629
NciI CCSGG 2 cut(s) 94, 475
NdeII GATC 6 cut(s) 64, 96, 224, 352, 363, 455
NlaIII CATG 2 cut(s) 247, 259
NlaIV GGNNCC 4 cut(s) 78, 98, 121, 365
NmeAIII GCCGAG 2 cut(s) 284, 372
NruI TCGCGA 1 cut(s) 482
PfeI GAWTC 1 cut(s) 484
PfoI TCCNGGA 1 cut(s) 92
PkrI GCNGC 2 cut(s) 345, 631
Ple19I CGATCG 2 cut(s) 227, 355
PleI GAGTC 1 cut(s) 193
PpsI GAGTC 1 cut(s) 193
PpuMI RGGWCCY 1 cut(s) 198
Psp124BI GAGCTC 2 cut(s) 161, 374
Psp5II RGGWCCY 1 cut(s) 198
Psp6I CCWGG 1 cut(s) 329
PspFI CCCAGC 1 cut(s) 324
PspGI CCWGG 1 cut(s) 329
PspN4I GGNNCC 4 cut(s) 78, 98, 121, 365
PspPI GGNCC 3 cut(s) 76, 198, 327
PspPPI RGGWCCY 1 cut(s) 198
PsuI RGATCY 3 cut(s) 96, 363, 455
PvuI CGATCG 2 cut(s) 227, 355
RgaI GCGATCGC 1 cut(s) 355
RruI TCGCGA 1 cut(s) 482
SacI GAGCTC 2 cut(s) 161, 374
SaqAI TTAA 1 cut(s) 548
SatI GCNGC 2 cut(s) 344, 630
Sau3AI GATC 6 cut(s) 64, 96, 224, 352, 363, 455
Sau96I GGNCC 3 cut(s) 76, 198, 327
SchI GAGTC 1 cut(s) 194
ScrFI CCNGG 3 cut(s) 94, 331, 475
SduI GDGCHC 2 cut(s) 161, 374
SetI ASST 8 cut(s) 92, 161, 203, 221, 263, 299, 326, 374
SfaAI GCGATCGC 1 cut(s) 355
SfaNI GCATC 1 cut(s) 126
SgfI GCGATCGC 1 cut(s) 355
SinI GGWCC 2 cut(s) 76, 198
Sse9I AATT 4 cut(s) 124, 549, 583, 610
SsiI CCGC 4 cut(s) 214, 290, 344, 629
SstI GAGCTC 2 cut(s) 161, 374
StyD4I CCNGG 3 cut(s) 92, 329, 473
TaaI ACNGT 1 cut(s) 15
TaqI TCGA 7 cut(s) 107, 194, 227, 263, 281, 407, 432
TasI AATT 4 cut(s) 124, 549, 583, 610
TauI GCSGC 2 cut(s) 346, 632
TfiI GAWTC 1 cut(s) 484
Tru1I TTAA 1 cut(s) 548
Tru9I TTAA 1 cut(s) 548
TspDTI ATGAA 3 cut(s) 63, 232, 418
TspGWI ACGGA 2 cut(s) 182, 393
VpaK11BI GGWCC 2 cut(s) 76, 198
XagI CCTNNNNNAGG 1 cut(s) 193
XapI RAATTY 1 cut(s) 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.