pycom04g09470

Belongs to the short-chain dehydrogenases reductases (SDR) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
N/A
Physical Location & Seq
Reverse (-)
12094556 .. 12096392
1837 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1014 bp
ATGTCGCTATTCACATGGCGGATGGGCGTCCACCAACCCAATTCCAATTCCGGGCTTCTCGGTTACATGCACTTCAACACGTTCCCCCCTGAGCTGTCTAGTATGCAGAACCACTCACCCCACCAAACCGCGGCGCCCTCTCCCCCACCCACCCCGCCCCCGCCACCCACCTGCTCCATCAATTGCCACAACAATAAAAAAACAACTCAAGACGACTCCACAAAGTCTCCTACTTCCTCACAATCTTCGTATGCTGTTGTGACAGGGGCTAACAAAGGGGTTGGATTTGGCACAGTTAAGCAGTTGGCTTCAAATGGGATCATGGTGGTGTTAACTGCTAGAGATGAGAAGAGGGGTCTTGAAGCTCTTGAAAAATTGAAAGAGTTTGGCGTCTCCGACCGGGTGGTTTTTCATCAGCTTGATGTAACAAATTCTGCTAGCATTGCTTCCCTTGCAGATTTTGTGAAAACCCAATTCGGAAAACTCGATATCTTGGTAAACAGTGCAGGAATTAATGGTAGCATAGTAAACCCTGAAAGTTTTAGATCAGCTGTAATTGGTAAGATGCCTGATGAAATCAATTGGAGTGAAATATCGATGACACCAAACTATGAGTTAGCAGAAGAATGCCTGAAAACAAACTACTATGGTCCCAAAAGTGTGACTGAGGCGCTTTTACCCCTCCTCAAGCTATCTGATTCGCCAAGAATCATTAATGTTTCTTCCGGTGCTGCTAAAGAGCATCCTCGCAAGATCAGATATACAAAGACAAGGATAATTAGGACACCAATAGACGCCACCACTCCGGAAGTGTCGTCCTACCTATTAGTGCAAAGAACACCCCACCCTTACAAAGGAGGACATGGAATACTGTCTTTGTTCAAAATGCCCACTAACGAAGATGGTGGTCTTACAACCCAGCTGAACTCGCACATCTTCGGAGAAATATACCTTGCGACAAAATCCATCGCCATATTTGCTGGTCATGGAGTCCACGACCAGGAGCATGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00902 ko01110 map00902 map01110
Pfam Domains
Protein Families
adh_short adh_short_C2

Protein Analysis

338

Amino Acids

36.71

Weight (kDa)

7.79

Isoelectric Point (pI)

41.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
adh_short PF00106 85 - 248 2.6e-25 short chain dehydrogenase
KR PF08659 86 - 171 8e-06 KR domain
adh_short_C2 PF13561 89 - 185 1.4e-15 Enoyl-(Acyl carrier protein) reductase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 179
Acc36I ACCTGC 1 cut(s) 179
AccB1I GGYRCC 1 cut(s) 133
AccII CGCG 1 cut(s) 131
AccIII TCCGGA 1 cut(s) 805
AciI CCGC 5 cut(s) 19, 129, 131, 155, 161
AclWI GGATC 1 cut(s) 326
AcsI RAATTY 1 cut(s) 430
AcyI GRCGYC 4 cut(s) 27, 134, 390, 795
AfiI CCNNNNNNNGG 4 cut(s) 51, 130, 854, 1000
AflIII ACRYGT 1 cut(s) 78
AgsI TTSAA 6 cut(s) 76, 312, 362, 371, 379, 883
AjnI CCWGG 1 cut(s) 999
AluBI AGCT 6 cut(s) 94, 365, 418, 551, 691, 922
AluI AGCT 6 cut(s) 94, 365, 418, 551, 691, 922
Alw26I GTCTC 2 cut(s) 231, 397
AlwI GGATC 1 cut(s) 326
Aor13HI TCCGGA 1 cut(s) 805
ApeKI GCWGC 1 cut(s) 731
ApoI RAATTY 1 cut(s) 430
AseI ATTAAT 2 cut(s) 513, 714
AspLEI GCGC 2 cut(s) 136, 673
AspS9I GGNCC 1 cut(s) 650
AsuC2I CCSGG 2 cut(s) 52, 401
AsuHPI GGTGA 1 cut(s) 108
AsuNHI GCTAGC 1 cut(s) 437
AvaII GGWCC 1 cut(s) 650
BanI GGYRCC 1 cut(s) 133
BbvI GCAGC 1 cut(s) 718
BccI CCATC 4 cut(s) 16, 185, 896, 974
BciT130I CCWGG 1 cut(s) 1001
BcnI CCSGG 2 cut(s) 52, 401
BcoDI GTCTC 2 cut(s) 231, 397
BfaI CTAG 3 cut(s) 99, 339, 438
BfoI RGCGCY 2 cut(s) 137, 674
BfuAI ACCTGC 1 cut(s) 179
BisI GCNGC 2 cut(s) 132, 732
BlsI GCNGC 2 cut(s) 133, 733
Bme1390I CCNGG 3 cut(s) 52, 401, 1001
Bme18I GGWCC 1 cut(s) 650
BmgT120I GGNCC 1 cut(s) 650
BmiI GGNNCC 2 cut(s) 135, 652
BmrFI CCNGG 3 cut(s) 52, 401, 1001
BmsI GCATC 2 cut(s) 555, 751
BmtI GCTAGC 1 cut(s) 441
Bpu10I CCTNAGC 1 cut(s) 90
BpuEI CTTGAG 2 cut(s) 192, 671
BpuMI CCSGG 2 cut(s) 52, 401
Bsa29I ATCGAT 1 cut(s) 596
BsaHI GRCGYC 4 cut(s) 27, 134, 390, 795
BsaJI CCNNGG 1 cut(s) 129
BsaWI WCCGGW 2 cut(s) 725, 805
BsaXI ACNNNNNCTCC 2 cut(s) 211, 241
Bsc4I CCNNNNNNNGG 4 cut(s) 51, 130, 854, 1000
Bse3DI GCAATG 1 cut(s) 441
BseAI TCCGGA 1 cut(s) 805
BseBI CCWGG 1 cut(s) 1001
BseCI ATCGAT 1 cut(s) 596
BseDI CCNNGG 1 cut(s) 129
BseGI GGATG 2 cut(s) 27, 742
BseLI CCNNNNNNNGG 4 cut(s) 51, 130, 854, 1000
BseMI GCAATG 1 cut(s) 441
BseMII CTCAG 2 cut(s) 81, 657
BseRI GAGGAG 1 cut(s) 674
BseXI GCAGC 1 cut(s) 718
BseYI CCCAGC 1 cut(s) 918
BsgI GTGCAG 1 cut(s) 525
Bsh1236I CGCG 1 cut(s) 131
Bsh1285I CGRYCG 1 cut(s) 400
BshNI GGYRCC 1 cut(s) 133
BshVI ATCGAT 1 cut(s) 596
BsiEI CGRYCG 1 cut(s) 400
BsiSI CCGG 4 cut(s) 51, 400, 726, 806
BslFI GGGAC 1 cut(s) 636
BslI CCNNNNNNNGG 4 cut(s) 51, 130, 854, 1000
BsmAI GTCTC 2 cut(s) 231, 397
BsmBI CGTCTC 1 cut(s) 397
BsmFI GGGAC 1 cut(s) 636
BsmI GAATGC 1 cut(s) 632
Bsp13I TCCGGA 1 cut(s) 805
Bsp143I GATC 3 cut(s) 318, 545, 753
BspACI CCGC 5 cut(s) 19, 129, 131, 155, 161
BspCNI CTCAG 2 cut(s) 82, 658
BspDI ATCGAT 1 cut(s) 596
BspEI TCCGGA 1 cut(s) 805
BspFNI CGCG 1 cut(s) 131
BspLI GGNNCC 2 cut(s) 135, 652
BspMI ACCTGC 1 cut(s) 179
BspOI GCTAGC 1 cut(s) 441
BspPI GGATC 1 cut(s) 326
BspT107I GGYRCC 1 cut(s) 133
BsrDI GCAATG 1 cut(s) 441
BssECI CCNNGG 1 cut(s) 129
BssMI GATC 3 cut(s) 318, 545, 753
BssNI GRCGYC 4 cut(s) 27, 134, 390, 795
Bst2UI CCWGG 1 cut(s) 1001
Bst4CI ACNGT 3 cut(s) 295, 503, 873
Bst6I CTCTTC 1 cut(s) 344
BstACI GRCGYC 4 cut(s) 27, 134, 390, 795
BstC8I GCNNGC 1 cut(s) 439
BstDEI CTNAG 2 cut(s) 90, 666
BstDSI CCRYGG 1 cut(s) 129
BstENI CCTNNNNNAGG 1 cut(s) 852
BstF5I GGATG 2 cut(s) 27, 742
BstFNI CGCG 1 cut(s) 131
BstH2I RGCGCY 2 cut(s) 137, 674
BstHHI GCGC 2 cut(s) 136, 673
BstKTI GATC 3 cut(s) 321, 548, 756
BstMAI GTCTC 2 cut(s) 231, 397
BstMBI GATC 3 cut(s) 318, 545, 753
BstMCI CGRYCG 1 cut(s) 400
BstMWI GCNNNNNNNGC 4 cut(s) 443, 452, 928, 977
BstNI CCWGG 1 cut(s) 1001
BstNSI RCATGY 1 cut(s) 70
BstSCI CCNGG 3 cut(s) 50, 399, 999
BstUI CGCG 1 cut(s) 131
BstV1I GCAGC 1 cut(s) 718
Bsu15I ATCGAT 1 cut(s) 596
BsuTUI ATCGAT 1 cut(s) 596
BtgI CCRYGG 1 cut(s) 129
BtgZI GCGATG 1 cut(s) 952
BtsCI GGATG 2 cut(s) 27, 742
BtsIMutI CAGTG 1 cut(s) 508
BveI ACCTGC 1 cut(s) 179
Cac8I GCNNGC 1 cut(s) 439
CfoI GCGC 2 cut(s) 136, 673
Cfr13I GGNCC 1 cut(s) 650
Cfr42I CCGCGG 1 cut(s) 132
ClaI ATCGAT 1 cut(s) 596
CseI GACGC 3 cut(s) 16, 379, 803
CviAII CATG 6 cut(s) 15, 67, 322, 863, 986, 1007
CviJI RGCY 9 cut(s) 55, 94, 269, 308, 365, 418, 551, 691, 922
CviKI_1 RGCY 9 cut(s) 55, 94, 269, 308, 365, 418, 551, 691, 922
DdeI CTNAG 2 cut(s) 90, 666
DinI GGCGCC 1 cut(s) 135
DpnI GATC 3 cut(s) 320, 547, 755
DpnII GATC 3 cut(s) 318, 545, 753
Eam1104I CTCTTC 1 cut(s) 344
EarI CTCTTC 1 cut(s) 344
EciI GGCGGA 1 cut(s) 34
Eco32I GATATC 1 cut(s) 490
Eco47I GGWCC 1 cut(s) 650
EcoNI CCTNNNNNAGG 1 cut(s) 852
EcoRII CCWGG 1 cut(s) 999
EcoRV GATATC 1 cut(s) 490
EgeI GGCGCC 1 cut(s) 135
EheI GGCGCC 1 cut(s) 135
Esp3I CGTCTC 1 cut(s) 397
FaeI CATG 6 cut(s) 18, 70, 325, 866, 989, 1010
FaqI GGGAC 1 cut(s) 636
FatI CATG 6 cut(s) 14, 66, 321, 862, 985, 1006
FauI CCCGC 2 cut(s) 162, 168
Fnu4HI GCNGC 2 cut(s) 132, 732
FokI GGATG 2 cut(s) 34, 729
Fsp4HI GCNGC 2 cut(s) 132, 732
FspBI CTAG 3 cut(s) 99, 339, 438
GlaI GCGC 2 cut(s) 135, 672
GluI GCNGC 2 cut(s) 132, 732
GsaI CCCAGC 1 cut(s) 922
HaeII RGCGCY 2 cut(s) 137, 674
HapII CCGG 4 cut(s) 51, 400, 726, 806
HgaI GACGC 3 cut(s) 16, 379, 803
HhaI GCGC 2 cut(s) 136, 673
Hin1I GRCGYC 4 cut(s) 27, 134, 390, 795
Hin1II CATG 6 cut(s) 18, 70, 325, 866, 989, 1010
Hin6I GCGC 2 cut(s) 134, 671
HinP1I GCGC 2 cut(s) 134, 671
HincII GTYRAC 1 cut(s) 333
HindII GTYRAC 1 cut(s) 333
HinfI GANTC 4 cut(s) 215, 698, 708, 990
HpaI GTTAAC 1 cut(s) 333
HpaII CCGG 4 cut(s) 51, 400, 726, 806
HphI GGTGA 1 cut(s) 108
Hpy166II GTNNAC 5 cut(s) 31, 333, 499, 529, 994
Hpy188I TCNGA 5 cut(s) 397, 479, 697, 758, 941
Hpy188III TCNNGA 4 cut(s) 209, 359, 368, 806
Hpy8I GTNNAC 5 cut(s) 31, 333, 499, 529, 994
HpyCH4III ACNGT 3 cut(s) 295, 503, 873
HpyCH4IV ACGT 1 cut(s) 80
HpyCH4V TGCA 5 cut(s) 70, 106, 455, 506, 832
HpyF10VI GCNNNNNNNGC 4 cut(s) 443, 452, 928, 977
HpyF3I CTNAG 2 cut(s) 90, 666
HpySE526I ACGT 1 cut(s) 80
Hsp92I GRCGYC 4 cut(s) 27, 134, 390, 795
Hsp92II CATG 6 cut(s) 18, 70, 325, 866, 989, 1010
HspAI GCGC 2 cut(s) 134, 671
KasI GGCGCC 1 cut(s) 133
Kpn2I TCCGGA 1 cut(s) 805
KspAI GTTAAC 1 cut(s) 333
KspI CCGCGG 1 cut(s) 132
Kzo9I GATC 3 cut(s) 318, 545, 753
LmnI GCTCC 2 cut(s) 179, 1003
Lsp1109I GCAGC 1 cut(s) 718
LweI GCATC 2 cut(s) 555, 751
MaeI CTAG 3 cut(s) 99, 339, 438
MaeII ACGT 1 cut(s) 80
MaeIII GTNAC 4 cut(s) 62, 259, 424, 661
MalI GATC 3 cut(s) 320, 547, 755
MboI GATC 3 cut(s) 318, 545, 753
MboII GAAGA 6 cut(s) 237, 361, 635, 714, 911, 928
MfeI CAATTG 2 cut(s) 181, 580
Mly113I GGCGCC 1 cut(s) 134
MlyI GAGTC 2 cut(s) 209, 999
MmeI TCCRAC 2 cut(s) 262, 420
MnlI CCTC 8 cut(s) 148, 247, 345, 661, 692, 695, 756, 851
MroI TCCGGA 1 cut(s) 805
MseI TTAA 4 cut(s) 297, 332, 513, 714
MslI CAYNNNNRTG 1 cut(s) 326
MspA1I CMGCKG 3 cut(s) 131, 551, 922
MspI CCGG 4 cut(s) 51, 400, 726, 806
MspR9I CCNGG 3 cut(s) 52, 401, 1001
MunI CAATTG 2 cut(s) 181, 580
Mva1269I GAATGC 1 cut(s) 632
MvaI CCWGG 1 cut(s) 1001
MvnI CGCG 1 cut(s) 131
MwoI GCNNNNNNNGC 4 cut(s) 443, 452, 928, 977
NarI GGCGCC 1 cut(s) 134
NciI CCSGG 2 cut(s) 52, 401
NdeII GATC 3 cut(s) 318, 545, 753
NheI GCTAGC 1 cut(s) 437
NlaIII CATG 6 cut(s) 18, 70, 325, 866, 989, 1010
NlaIV GGNNCC 2 cut(s) 135, 652
NmuCI GTSAC 2 cut(s) 259, 661
NspI RCATGY 1 cut(s) 70
PaqCI CACCTGC 1 cut(s) 179
PcsI WCGNNNNNNNCGW 1 cut(s) 483
PctI GAATGC 1 cut(s) 632
PfeI GAWTC 2 cut(s) 698, 708
PkrI GCNGC 2 cut(s) 133, 733
PleI GAGTC 2 cut(s) 209, 998
PluTI GGCGCC 1 cut(s) 137
PpsI GAGTC 2 cut(s) 209, 998
PshBI ATTAAT 2 cut(s) 513, 714
Psp6I CCWGG 1 cut(s) 999
PspFI CCCAGC 1 cut(s) 918
PspGI CCWGG 1 cut(s) 999
PspN4I GGNNCC 2 cut(s) 135, 652
PspPI GGNCC 1 cut(s) 650
PvuII CAGCTG 2 cut(s) 551, 922
RseI CAYNNNNRTG 1 cut(s) 326
SacII CCGCGG 1 cut(s) 132
SaqAI TTAA 4 cut(s) 297, 332, 513, 714
SatI GCNGC 2 cut(s) 132, 732
Sau3AI GATC 3 cut(s) 318, 545, 753
Sau96I GGNCC 1 cut(s) 650
SchI GAGTC 2 cut(s) 209, 999
ScrFI CCNGG 3 cut(s) 52, 401, 1001
SfaNI GCATC 2 cut(s) 555, 751
SfoI GGCGCC 1 cut(s) 135
Sfr303I CCGCGG 1 cut(s) 132
SgrBI CCGCGG 1 cut(s) 132
SinI GGWCC 1 cut(s) 650
SmiMI CAYNNNNRTG 1 cut(s) 326
SmlI CTYRAG 2 cut(s) 207, 686
SmoI CTYRAG 2 cut(s) 207, 686
SsiI CCGC 5 cut(s) 19, 129, 131, 155, 161
SspDI GGCGCC 1 cut(s) 133
SspMI CTAG 3 cut(s) 99, 339, 438
StyD4I CCNGG 3 cut(s) 50, 399, 999
TaaI ACNGT 3 cut(s) 295, 503, 873
TaiI ACGT 1 cut(s) 83
TaqI TCGA 2 cut(s) 486, 596
TauI GCSGC 1 cut(s) 134
TfiI GAWTC 2 cut(s) 698, 708
Tru1I TTAA 4 cut(s) 297, 332, 513, 714
Tru9I TTAA 4 cut(s) 297, 332, 513, 714
TscAI CASTG 1 cut(s) 508
TseFI GTSAC 2 cut(s) 259, 661
TseI GCWGC 1 cut(s) 731
Tsp45I GTSAC 2 cut(s) 259, 661
TspDTI ATGAA 2 cut(s) 401, 588
TspRI CASTG 1 cut(s) 508
VpaK11BI GGWCC 1 cut(s) 650
VspI ATTAAT 2 cut(s) 513, 714
XagI CCTNNNNNAGG 1 cut(s) 852
XapI RAATTY 1 cut(s) 430
XceI RCATGY 1 cut(s) 70
XspI CTAG 3 cut(s) 99, 339, 438
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.