pycom04g13080

At1g04910-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
N/A
Physical Location & Seq
Reverse (-)
16092312 .. 16094083
1772 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 1113 bp
ATGTCCTCCGGTGGGACGAACTGCTCAAGCAACATTAGTTCGAAACAACCGGCTCTCCTCGCGCTGCCCACGACCACCAGACGACGCGTCGCTGACGGCGTCATGGACGCCGGCGAGCGGCTCCCGACGAGCACTCTCCCTCCCGACAGCAACATTGTCTTCGCCGACGACGACGATGTGGAAAACGACAGCCTCCTCGACGGCACTATATGCGGGCCCCACCACGCCCTCCACCACAACCACAACCATTATTCGCACCACCCCGTGATGAAGTACCTCGTTCTCCGTCGGCGGCTATTCTTCTGCGTTCCCGACAAGTGGCTGCTCCCGATCGAGGACGGATTTCTATGGACGGTCGCGATGTTGCAGTCGCTGAGATCGGGGCGGAACGTGGTCCGCAATATTTTCGGGGCTCTGCTTTTGATGGCGGTTATCTCCGCGTTCGTGAAGTTGTCGTTTTTTAGTAGCCACGTCGAGGTGGTCGGGGAGGTGGCACAGAGGACGGAGGGCCGACTTTTGATTCTGAATACGGTGAAGGACGATTGGTCGGGGGCGCAGCGGGCCGTCGCCGAGAACCAGGAGTCCAATTCCATGCCTAAACGTGTTTTGGAGAGGGAGAGGTTGTCGAAAACCGAATCCTCCACCATGTTCAAGCGACTTCTCGAGCGGTTGCCGACCCCAGAGATTTGGAGGAAACCCAACAGCGACAATCTTGTTAAATGCATAGCTCGACCCAAGAACCGAATAAGGACAGGATCGAAAACAAATGGTTATCTGCTTGTTCATGCCAATGGCGGATTGAATCAGATGAGAACAGGGATATGTGATATGGTAGCCGTTGCAAAGATAATGAATGCTACCCTGGTTCTTCCACTGATGGACCATAAATCTTTTTGGACGGATCCAAGTGATTTTAAAGACATTTTTGACTGGAGACACTTCATGAAAGTATTGAAAGATGATATTGATATTATCGAGTATTTGCCAACCAAATATGCAGCTGTGAAGCCTGTTCTGAAGGCTCCCGTCTCCTGGTCGAAGGCGAGCTATTACAGAAGCGAGATCGATCCCCTTGCTGAAAAAGCACAAGGTTATAAAGTTTACGCACACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0044424 GO:0044464
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
O-FucT

Protein Analysis

371

Amino Acids

41.77

Weight (kDa)

9.16

Isoelectric Point (pI)

47.32

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
O-FucT PF10250 258 - 363 3.3e-33 GDP-fucose protein O-fucosyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1095
AccBSI CCGCTC 2 cut(s) 118, 667
AccII CGCG 4 cut(s) 62, 87, 359, 440
AclWI GGATC 4 cut(s) 763, 896, 909, 1061
AcuI CTGAAG 1 cut(s) 1037
AcyI GRCGYC 2 cut(s) 99, 108
AdeI CACNNNGTG 1 cut(s) 265
AfaI GTAC 1 cut(s) 275
AfiI CCNNNNNNNGG 6 cut(s) 12, 117, 318, 334, 475, 1032
AflIII ACRYGT 2 cut(s) 85, 601
AgsI TTSAA 3 cut(s) 652, 802, 955
AhdI GACNNNNNGTC 2 cut(s) 86, 544
AjiI CACGTC 1 cut(s) 472
AjnI CCWGG 3 cut(s) 576, 861, 1031
AloI GAACNNNNNNTCC 2 cut(s) 566, 598
AluBI AGCT 3 cut(s) 728, 1001, 1047
AluI AGCT 3 cut(s) 728, 1001, 1047
Alw21I GWGCWC 1 cut(s) 134
Alw26I GTCTC 2 cut(s) 928, 1033
AlwI GGATC 4 cut(s) 763, 896, 909, 1061
AlwNI CAGNNNCTG 1 cut(s) 373
Ama87I CYCGRG 1 cut(s) 662
AoxI GGCC 3 cut(s) 215, 508, 561
ApaI GGGCCC 1 cut(s) 219
ApeKI GCWGC 4 cut(s) 64, 322, 556, 998
AspLEI GCGC 2 cut(s) 64, 556
AspS9I GGNCC 6 cut(s) 215, 216, 394, 508, 561, 880
AsuHPI GGTGA 1 cut(s) 544
AsuII TTCGAA 1 cut(s) 41
AvaI CYCGRG 1 cut(s) 662
AvaII GGWCC 2 cut(s) 394, 880
BaeGI GKGCMC 1 cut(s) 219
BamHI GGATCC 1 cut(s) 901
BanII GRGCYC 2 cut(s) 219, 415
BbsI GAAGAC 1 cut(s) 151
Bbv12I GWGCWC 1 cut(s) 134
BbvI GCAGC 4 cut(s) 51, 309, 568, 1010
BccI CCATC 2 cut(s) 418, 871
BceAI ACGGC 4 cut(s) 112, 217, 548, 821
BcgI CGANNNNNNTGC 4 cut(s) 388, 422, 1055, 1089
BciT130I CCWGG 3 cut(s) 578, 863, 1033
BcoDI GTCTC 2 cut(s) 928, 1033
BisI GCNGC 6 cut(s) 65, 119, 293, 323, 557, 999
BlsI GCNGC 6 cut(s) 66, 120, 294, 324, 558, 1000
Bme1390I CCNGG 3 cut(s) 578, 863, 1033
Bme18I GGWCC 2 cut(s) 394, 880
BmeRI GACNNNNNGTC 2 cut(s) 86, 544
BmeT110I CYCGRG 1 cut(s) 662
BmgBI CACGTC 1 cut(s) 472
BmgT120I GGNCC 6 cut(s) 215, 216, 394, 508, 561, 880
BmiI GGNNCC 5 cut(s) 122, 217, 218, 903, 1023
BmrFI CCNGG 3 cut(s) 578, 863, 1033
BpiI GAAGAC 1 cut(s) 151
BpmI CTGGAG 1 cut(s) 952
Bpu14I TTCGAA 1 cut(s) 41
BpuEI CTTGAG 1 cut(s) 10
Bsa29I ATCGAT 1 cut(s) 1065
BsaHI GRCGYC 2 cut(s) 99, 108
BsaJI CCNNGG 1 cut(s) 861
BsaWI WCCGGW 1 cut(s) 8
BsaXI ACNNNNNCTCC 6 cut(s) 39, 69, 124, 154, 608, 638
Bsc4I CCNNNNNNNGG 6 cut(s) 12, 117, 318, 334, 475, 1032
Bse118I RCCGGY 2 cut(s) 49, 110
Bse1I ACTGG 1 cut(s) 935
BseBI CCWGG 3 cut(s) 578, 863, 1033
BseCI ATCGAT 1 cut(s) 1065
BseDI CCNNGG 1 cut(s) 861
BseLI CCNNNNNNNGG 6 cut(s) 12, 117, 318, 334, 475, 1032
BseMII CTCAG 1 cut(s) 365
BseNI ACTGG 1 cut(s) 935
BseRI GAGGAG 2 cut(s) 47, 185
BseSI GKGCMC 1 cut(s) 219
BseXI GCAGC 4 cut(s) 51, 309, 568, 1010
Bsh1236I CGCG 4 cut(s) 62, 87, 359, 440
Bsh1285I CGRYCG 2 cut(s) 333, 357
BshFI GGCC 3 cut(s) 217, 510, 563
BshVI ATCGAT 1 cut(s) 1065
BsiEI CGRYCG 2 cut(s) 333, 357
BsiHKAI GWGCWC 1 cut(s) 134
BsiHKCI CYCGRG 1 cut(s) 662
BsiSI CCGG 3 cut(s) 9, 50, 111
BslFI GGGAC 1 cut(s) 28
BslI CCNNNNNNNGG 6 cut(s) 12, 117, 318, 334, 475, 1032
BsmAI GTCTC 2 cut(s) 928, 1033
BsmBI CGTCTC 1 cut(s) 1033
BsmFI GGGAC 1 cut(s) 28
BsmI GAATGC 1 cut(s) 859
BsnI GGCC 3 cut(s) 217, 510, 563
BsoBI CYCGRG 1 cut(s) 662
Bsp119I TTCGAA 1 cut(s) 41
Bsp120I GGGCCC 1 cut(s) 215
Bsp1286I GDGCHC 3 cut(s) 134, 219, 415
Bsp143I GATC 6 cut(s) 330, 377, 755, 901, 1062, 1066
Bsp68I TCGCGA 1 cut(s) 359
BspANI GGCC 3 cut(s) 217, 510, 563
BspCNI CTCAG 1 cut(s) 366
BspDI ATCGAT 1 cut(s) 1065
BspFNI CGCG 4 cut(s) 62, 87, 359, 440
BspHI TCATGA 1 cut(s) 942
BspLI GGNNCC 5 cut(s) 122, 217, 218, 903, 1023
BspPI GGATC 4 cut(s) 763, 896, 909, 1061
BspT104I TTCGAA 1 cut(s) 41
BsrBI CCGCTC 2 cut(s) 118, 667
BsrFI RCCGGY 2 cut(s) 49, 110
BsrI ACTGG 1 cut(s) 935
BssAI RCCGGY 2 cut(s) 49, 110
BssECI CCNNGG 1 cut(s) 861
BssMI GATC 6 cut(s) 330, 377, 755, 901, 1062, 1066
BssNI GRCGYC 2 cut(s) 99, 108
Bst2UI CCWGG 3 cut(s) 578, 863, 1033
Bst4CI ACNGT 2 cut(s) 355, 532
BstACI GRCGYC 2 cut(s) 99, 108
BstAPI GCANNNNNTGC 1 cut(s) 210
BstBI TTCGAA 1 cut(s) 41
BstC8I GCNNGC 5 cut(s) 112, 116, 215, 561, 1045
BstDEI CTNAG 1 cut(s) 374
BstFNI CGCG 4 cut(s) 62, 87, 359, 440
BstHHI GCGC 2 cut(s) 64, 556
BstKTI GATC 6 cut(s) 333, 380, 758, 904, 1065, 1069
BstMAI GTCTC 2 cut(s) 928, 1033
BstMBI GATC 6 cut(s) 330, 377, 755, 901, 1062, 1066
BstMCI CGRYCG 2 cut(s) 333, 357
BstMWI GCNNNNNNNGC 4 cut(s) 59, 210, 560, 1082
BstNI CCWGG 3 cut(s) 578, 863, 1033
BstSCI CCNGG 3 cut(s) 576, 861, 1031
BstSLI GKGCMC 1 cut(s) 219
BstUI CGCG 4 cut(s) 62, 87, 359, 440
BstV1I GCAGC 4 cut(s) 51, 309, 568, 1010
BstV2I GAAGAC 1 cut(s) 151
BstX2I RGATCY 1 cut(s) 901
BstXI CCANNNNNNTGG 1 cut(s) 687
BstYI RGATCY 1 cut(s) 901
Bsu15I ATCGAT 1 cut(s) 1065
BsuRI GGCC 3 cut(s) 217, 510, 563
BsuTUI ATCGAT 1 cut(s) 1065
BtgZI GCGATG 1 cut(s) 374
BtrI CACGTC 1 cut(s) 472
BtsIMutI CAGTG 2 cut(s) 872, 1108
BtuMI TCGCGA 1 cut(s) 359
Cac8I GCNNGC 5 cut(s) 112, 116, 215, 561, 1045
CaiI CAGNNNCTG 1 cut(s) 373
CciI TCATGA 1 cut(s) 942
CfoI GCGC 2 cut(s) 64, 556
Cfr10I RCCGGY 2 cut(s) 49, 110
Cfr13I GGNCC 6 cut(s) 215, 216, 394, 508, 561, 880
ClaI ATCGAT 1 cut(s) 1065
CseI GACGC 4 cut(s) 76, 88, 93, 116
Csp6I GTAC 1 cut(s) 274
CviAII CATG 5 cut(s) 103, 592, 646, 785, 943
CviQI GTAC 1 cut(s) 274
DdeI CTNAG 1 cut(s) 374
DpnI GATC 6 cut(s) 332, 379, 757, 903, 1064, 1068
DpnII GATC 6 cut(s) 330, 377, 755, 901, 1062, 1066
DraI TTTAAA 1 cut(s) 916
DraIII CACNNNGTG 1 cut(s) 265
DriI GACNNNNNGTC 2 cut(s) 86, 544
Eam1105I GACNNNNNGTC 2 cut(s) 86, 544
EciI GGCGGA 2 cut(s) 400, 810
Eco24I GRGCYC 2 cut(s) 219, 415
Eco47I GGWCC 2 cut(s) 394, 880
Eco57I CTGAAG 1 cut(s) 1037
Eco88I CYCGRG 1 cut(s) 662
EcoO109I RGGNCCY 1 cut(s) 216
EcoRII CCWGG 3 cut(s) 576, 861, 1031
EcoT22I ATGCAT 1 cut(s) 725
EcoT38I GRGCYC 2 cut(s) 219, 415
Esp3I CGTCTC 1 cut(s) 1033
FaeI CATG 5 cut(s) 106, 595, 649, 788, 946
FaqI GGGAC 1 cut(s) 28
FatI CATG 5 cut(s) 102, 591, 645, 784, 942
FauI CCCGC 2 cut(s) 206, 552
Fnu4HI GCNGC 6 cut(s) 65, 119, 293, 323, 557, 999
FriOI GRGCYC 2 cut(s) 219, 415
Fsp4HI GCNGC 6 cut(s) 65, 119, 293, 323, 557, 999
GlaI GCGC 2 cut(s) 63, 555
GluI GCNGC 6 cut(s) 65, 119, 293, 323, 557, 999
GsuI CTGGAG 1 cut(s) 952
HaeIII GGCC 3 cut(s) 217, 510, 563
HapII CCGG 3 cut(s) 9, 50, 111
HgaI GACGC 4 cut(s) 76, 88, 93, 116
HhaI GCGC 2 cut(s) 64, 556
Hin1I GRCGYC 2 cut(s) 99, 108
Hin1II CATG 5 cut(s) 106, 595, 649, 788, 946
Hin6I GCGC 2 cut(s) 62, 554
HinP1I GCGC 2 cut(s) 62, 554
HinfI GANTC 4 cut(s) 520, 581, 635, 802
HpaII CCGG 3 cut(s) 9, 50, 111
HphI GGTGA 1 cut(s) 544
Hpy166II GTNNAC 1 cut(s) 1102
Hpy188I TCNGA 3 cut(s) 525, 807, 1017
Hpy188III TCNNGA 8 cut(s) 124, 143, 311, 328, 358, 445, 662, 943
Hpy8I GTNNAC 1 cut(s) 1102
HpyAV CCTTC 3 cut(s) 529, 1012, 1033
HpyCH4III ACNGT 2 cut(s) 355, 532
HpyCH4IV ACGT 3 cut(s) 390, 471, 601
HpyCH4V TGCA 4 cut(s) 367, 723, 842, 998
HpyF10VI GCNNNNNNNGC 4 cut(s) 59, 210, 560, 1082
HpyF3I CTNAG 1 cut(s) 374
HpySE526I ACGT 3 cut(s) 390, 471, 601
Hsp92I GRCGYC 2 cut(s) 99, 108
Hsp92II CATG 5 cut(s) 106, 595, 649, 788, 946
HspAI GCGC 2 cut(s) 62, 554
KroI GCCGGC 1 cut(s) 110
KroNI GCCGGC 1 cut(s) 112
Kzo9I GATC 6 cut(s) 330, 377, 755, 901, 1062, 1066
LmnI GCTCC 3 cut(s) 126, 330, 1027
Lsp1109I GCAGC 4 cut(s) 51, 309, 568, 1010
MaeII ACGT 3 cut(s) 390, 471, 601
MalI GATC 6 cut(s) 332, 379, 757, 903, 1064, 1068
MbiI CCGCTC 2 cut(s) 118, 667
MboI GATC 6 cut(s) 330, 377, 755, 901, 1062, 1066
MboII GAAGA 3 cut(s) 151, 292, 860
MflI RGATCY 1 cut(s) 901
MhlI GDGCHC 3 cut(s) 134, 219, 415
MluCI AATT 1 cut(s) 586
MluI ACGCGT 1 cut(s) 85
MlyI GAGTC 1 cut(s) 590
Mph1103I ATGCAT 1 cut(s) 725
MreI CGCCGGCG 1 cut(s) 110
MroNI GCCGGC 1 cut(s) 110
MseI TTAA 2 cut(s) 717, 915
MslI CAYNNNNRTG 1 cut(s) 789
MspA1I CMGCKG 2 cut(s) 559, 1001
MspI CCGG 3 cut(s) 9, 50, 111
MspR9I CCNGG 3 cut(s) 578, 863, 1033
Mva1269I GAATGC 1 cut(s) 859
MvaI CCWGG 3 cut(s) 578, 863, 1033
MvnI CGCG 4 cut(s) 62, 87, 359, 440
MwoI GCNNNNNNNGC 4 cut(s) 59, 210, 560, 1082
NaeI GCCGGC 1 cut(s) 112
NdeII GATC 6 cut(s) 330, 377, 755, 901, 1062, 1066
NgoMIV GCCGGC 1 cut(s) 110
NlaIII CATG 5 cut(s) 106, 595, 649, 788, 946
NlaIV GGNNCC 5 cut(s) 122, 217, 218, 903, 1023
NmeAIII GCCGAG 1 cut(s) 595
NruI TCGCGA 1 cut(s) 359
NsiI ATGCAT 1 cut(s) 725
NspV TTCGAA 1 cut(s) 41
PaeR7I CTCGAG 1 cut(s) 662
PagI TCATGA 1 cut(s) 942
PcsI WCGNNNNNNNCGW 2 cut(s) 96, 168
PctI GAATGC 1 cut(s) 859
PdiI GCCGGC 1 cut(s) 112
PfeI GAWTC 3 cut(s) 520, 635, 802
PflFI GACNNNGTC 1 cut(s) 98
PkrI GCNGC 6 cut(s) 66, 120, 294, 324, 558, 1000
Ple19I CGATCG 1 cut(s) 333
PleI GAGTC 1 cut(s) 589
PpsI GAGTC 1 cut(s) 589
PsiI TTATAA 1 cut(s) 1095
Psp6I CCWGG 3 cut(s) 576, 861, 1031
PspGI CCWGG 3 cut(s) 576, 861, 1031
PspN4I GGNNCC 5 cut(s) 122, 217, 218, 903, 1023
PspOMI GGGCCC 1 cut(s) 215
PspPI GGNCC 6 cut(s) 215, 216, 394, 508, 561, 880
PstNI CAGNNNCTG 1 cut(s) 373
PsuI RGATCY 1 cut(s) 901
PsyI GACNNNGTC 1 cut(s) 98
PvuI CGATCG 1 cut(s) 333
PvuII CAGCTG 1 cut(s) 1001
RruI TCGCGA 1 cut(s) 359
RsaI GTAC 1 cut(s) 275
RsaNI GTAC 1 cut(s) 274
RseI CAYNNNNRTG 1 cut(s) 789
SaqAI TTAA 2 cut(s) 717, 915
SatI GCNGC 6 cut(s) 65, 119, 293, 323, 557, 999
Sau3AI GATC 6 cut(s) 330, 377, 755, 901, 1062, 1066
Sau96I GGNCC 6 cut(s) 215, 216, 394, 508, 561, 880
SchI GAGTC 1 cut(s) 590
ScrFI CCNGG 3 cut(s) 578, 863, 1033
SduI GDGCHC 3 cut(s) 134, 219, 415
Sfr274I CTCGAG 1 cut(s) 662
SfuI TTCGAA 1 cut(s) 41
SgrAI CRCCGGYG 1 cut(s) 110
SinI GGWCC 2 cut(s) 394, 880
SlaI CTCGAG 1 cut(s) 662
SmiMI CAYNNNNRTG 1 cut(s) 789
SmlI CTYRAG 2 cut(s) 25, 662
SmoI CTYRAG 2 cut(s) 25, 662
Sse9I AATT 1 cut(s) 586
SspI AATATT 1 cut(s) 403
StyD4I CCNGG 3 cut(s) 576, 861, 1031
TaaI ACNGT 2 cut(s) 355, 532
TaiI ACGT 3 cut(s) 393, 474, 604
TasI AATT 1 cut(s) 586
TauI GCSGC 2 cut(s) 121, 295
TfiI GAWTC 3 cut(s) 520, 635, 802
Tru1I TTAA 2 cut(s) 717, 915
Tru9I TTAA 2 cut(s) 717, 915
TscAI CASTG 1 cut(s) 879
TseI GCWGC 4 cut(s) 64, 322, 556, 998
TspDTI ATGAA 5 cut(s) 284, 773, 866, 931, 959
TspGWI ACGGA 4 cut(s) 275, 354, 518, 914
TspRI CASTG 1 cut(s) 879
Tth111I GACNNNGTC 1 cut(s) 98
VpaK11BI GGWCC 2 cut(s) 394, 880
XhoI CTCGAG 1 cut(s) 662
Zsp2I ATGCAT 1 cut(s) 725
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.