pycom04g14020

SelT-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
N/A
Physical Location & Seq
Forward (+)
16931414 .. 16932174
761 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 222 bp
ATGGATCGATCGAGCTCAGTTAGTTCTGGTAGGGCGCCTGCTGGACAAACTGGTACCGGTTGTTCAGGTAGGAATCATTGGGACGATAATGGTCGCAAACATGTGTTCTCGAGGCTGAGAATAACGACGCCACCTCCTTGGTACCAGGCCTTGCAAGCAAATAGGTTTGGAAGTATGGCAAGCACTTGGCTTTTTGGAAACTTCATCCAATCCTTCCTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Rdx

Protein Analysis

74

Amino Acids

8.1

Weight (kDa)

11.27

Isoelectric Point (pI)

42.53

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 2 cut(s) 53, 141
AccB1I GGYRCC 3 cut(s) 34, 53, 141
AclWI GGATC 1 cut(s) 12
AcyI GRCGYC 2 cut(s) 35, 128
AfaI GTAC 2 cut(s) 55, 143
AflIII ACRYGT 1 cut(s) 100
AgeI ACCGGT 1 cut(s) 56
AjnI CCWGG 1 cut(s) 144
AluBI AGCT 1 cut(s) 15
AluI AGCT 1 cut(s) 15
Alw21I GWGCWC 1 cut(s) 17
AlwI GGATC 1 cut(s) 12
Ama87I CYCGRG 1 cut(s) 109
AoxI GGCC 1 cut(s) 147
AsiGI ACCGGT 1 cut(s) 56
Asp718I GGTACC 2 cut(s) 53, 141
AspLEI GCGC 1 cut(s) 37
AvaI CYCGRG 1 cut(s) 109
BanI GGYRCC 3 cut(s) 34, 53, 141
BanII GRGCYC 1 cut(s) 17
Bbv12I GWGCWC 1 cut(s) 17
BciT130I CCWGG 1 cut(s) 146
BfoI RGCGCY 1 cut(s) 38
Bme1390I CCNGG 1 cut(s) 146
BmeT110I CYCGRG 1 cut(s) 109
BmiI GGNNCC 3 cut(s) 36, 55, 143
BmrFI CCNGG 1 cut(s) 146
Bsa29I ATCGAT 1 cut(s) 7
BsaHI GRCGYC 2 cut(s) 35, 128
BsaJI CCNNGG 1 cut(s) 137
BsaWI WCCGGW 1 cut(s) 56
BsaXI ACNNNNNCTCC 2 cut(s) 118, 148
Bse118I RCCGGY 1 cut(s) 56
Bse1I ACTGG 1 cut(s) 55
BseBI CCWGG 1 cut(s) 146
BseCI ATCGAT 1 cut(s) 7
BseDI CCNNGG 1 cut(s) 137
BseGI GGATG 1 cut(s) 204
BseMII CTCAG 2 cut(s) 30, 107
BseNI ACTGG 1 cut(s) 55
Bsh1285I CGRYCG 1 cut(s) 11
BshFI GGCC 1 cut(s) 149
BshNI GGYRCC 3 cut(s) 34, 53, 141
BshTI ACCGGT 1 cut(s) 56
BshVI ATCGAT 1 cut(s) 7
BsiEI CGRYCG 1 cut(s) 11
BsiHKAI GWGCWC 1 cut(s) 17
BsiHKCI CYCGRG 1 cut(s) 109
BsiSI CCGG 1 cut(s) 57
BslFI GGGAC 1 cut(s) 95
BsmFI GGGAC 1 cut(s) 95
BsnI GGCC 1 cut(s) 149
BsoBI CYCGRG 1 cut(s) 109
Bsp1286I GDGCHC 1 cut(s) 17
Bsp143I GATC 2 cut(s) 4, 8
BspANI GGCC 1 cut(s) 149
BspCNI CTCAG 2 cut(s) 29, 108
BspDI ATCGAT 1 cut(s) 7
BspLI GGNNCC 3 cut(s) 36, 55, 143
BspPI GGATC 1 cut(s) 12
BspT107I GGYRCC 3 cut(s) 34, 53, 141
BsrFI RCCGGY 1 cut(s) 56
BsrI ACTGG 1 cut(s) 55
BssAI RCCGGY 1 cut(s) 56
BssECI CCNNGG 1 cut(s) 137
BssMI GATC 2 cut(s) 4, 8
BssNI GRCGYC 2 cut(s) 35, 128
BssT1I CCWWGG 1 cut(s) 137
Bst2UI CCWGG 1 cut(s) 146
BstACI GRCGYC 2 cut(s) 35, 128
BstC8I GCNNGC 3 cut(s) 39, 156, 181
BstDEI CTNAG 2 cut(s) 16, 116
BstF5I GGATG 1 cut(s) 204
BstH2I RGCGCY 1 cut(s) 38
BstHHI GCGC 1 cut(s) 37
BstKTI GATC 2 cut(s) 7, 11
BstMBI GATC 2 cut(s) 4, 8
BstMCI CGRYCG 1 cut(s) 11
BstMWI GCNNNNNNNGC 1 cut(s) 155
BstNI CCWGG 1 cut(s) 146
BstNSI RCATGY 1 cut(s) 104
BstSCI CCNGG 1 cut(s) 144
BstXI CCANNNNNNTGG 1 cut(s) 138
Bsu15I ATCGAT 1 cut(s) 7
BsuRI GGCC 1 cut(s) 149
BsuTUI ATCGAT 1 cut(s) 7
BtsCI GGATG 1 cut(s) 204
Cac8I GCNNGC 3 cut(s) 39, 156, 181
CfoI GCGC 1 cut(s) 37
Cfr10I RCCGGY 1 cut(s) 56
ClaI ATCGAT 1 cut(s) 7
CseI GACGC 1 cut(s) 136
Csp6I GTAC 2 cut(s) 54, 142
CspAI ACCGGT 1 cut(s) 56
CviAII CATG 1 cut(s) 101
CviJI RGCY 4 cut(s) 15, 115, 149, 190
CviKI_1 RGCY 4 cut(s) 15, 115, 149, 190
CviQI GTAC 2 cut(s) 54, 142
DdeI CTNAG 2 cut(s) 16, 116
DinI GGCGCC 1 cut(s) 36
DpnI GATC 2 cut(s) 6, 10
DpnII GATC 2 cut(s) 4, 8
Ecl136II GAGCTC 1 cut(s) 15
Eco130I CCWWGG 1 cut(s) 137
Eco147I AGGCCT 1 cut(s) 149
Eco24I GRGCYC 1 cut(s) 17
Eco53kI GAGCTC 1 cut(s) 15
Eco88I CYCGRG 1 cut(s) 109
EcoICRI GAGCTC 1 cut(s) 15
EcoRII CCWGG 1 cut(s) 144
EcoT14I CCWWGG 1 cut(s) 137
EcoT38I GRGCYC 1 cut(s) 17
EgeI GGCGCC 1 cut(s) 36
EheI GGCGCC 1 cut(s) 36
ErhI CCWWGG 1 cut(s) 137
FaeI CATG 1 cut(s) 104
FaiI YATR 2 cut(s) 102, 176
FaqI GGGAC 1 cut(s) 95
FatI CATG 1 cut(s) 100
FokI GGATG 1 cut(s) 191
FriOI GRGCYC 1 cut(s) 17
GlaI GCGC 1 cut(s) 36
HaeII RGCGCY 1 cut(s) 38
HaeIII GGCC 1 cut(s) 149
HapII CCGG 1 cut(s) 57
HgaI GACGC 1 cut(s) 136
HhaI GCGC 1 cut(s) 37
Hin1I GRCGYC 2 cut(s) 35, 128
Hin1II CATG 1 cut(s) 104
Hin6I GCGC 1 cut(s) 35
HinP1I GCGC 1 cut(s) 35
HinfI GANTC 1 cut(s) 73
HpaII CCGG 1 cut(s) 57
Hpy188III TCNNGA 1 cut(s) 109
Hpy99I CGWCG 1 cut(s) 130
HpyCH4V TGCA 1 cut(s) 154
HpyF10VI GCNNNNNNNGC 1 cut(s) 155
HpyF3I CTNAG 2 cut(s) 16, 116
Hsp92I GRCGYC 2 cut(s) 35, 128
Hsp92II CATG 1 cut(s) 104
HspAI GCGC 1 cut(s) 35
KasI GGCGCC 1 cut(s) 34
KpnI GGTACC 2 cut(s) 57, 145
Kzo9I GATC 2 cut(s) 4, 8
LpnPI CCDG 8 cut(s) 12, 27, 36, 51, 51, 70, 131, 158
MalI GATC 2 cut(s) 6, 10
MboI GATC 2 cut(s) 4, 8
MhlI GDGCHC 1 cut(s) 17
Mly113I GGCGCC 1 cut(s) 35
MnlI CCTC 2 cut(s) 105, 144
MseI TTAA 1 cut(s) 220
MspI CCGG 1 cut(s) 57
MspR9I CCNGG 1 cut(s) 146
MvaI CCWGG 1 cut(s) 146
MwoI GCNNNNNNNGC 1 cut(s) 155
NarI GGCGCC 1 cut(s) 35
NdeII GATC 2 cut(s) 4, 8
NlaIII CATG 1 cut(s) 104
NlaIV GGNNCC 3 cut(s) 36, 55, 143
NspI RCATGY 1 cut(s) 104
PaeR7I CTCGAG 1 cut(s) 109
PceI AGGCCT 1 cut(s) 149
PciI ACATGT 1 cut(s) 100
PfeI GAWTC 1 cut(s) 73
PinAI ACCGGT 1 cut(s) 56
Ple19I CGATCG 1 cut(s) 11
PluTI GGCGCC 1 cut(s) 38
PscI ACATGT 1 cut(s) 100
Psp124BI GAGCTC 1 cut(s) 17
Psp6I CCWGG 1 cut(s) 144
PspGI CCWGG 1 cut(s) 144
PspN4I GGNNCC 3 cut(s) 36, 55, 143
PsrI GAACNNNNNNTAC 2 cut(s) 46, 78
PvuI CGATCG 1 cut(s) 11
RsaI GTAC 2 cut(s) 55, 143
RsaNI GTAC 2 cut(s) 54, 142
SacI GAGCTC 1 cut(s) 17
SaqAI TTAA 1 cut(s) 220
Sau3AI GATC 2 cut(s) 4, 8
ScrFI CCNGG 1 cut(s) 146
SduI GDGCHC 1 cut(s) 17
SetI ASST 4 cut(s) 17, 70, 136, 167
SfoI GGCGCC 1 cut(s) 36
Sfr274I CTCGAG 1 cut(s) 109
SlaI CTCGAG 1 cut(s) 109
SmlI CTYRAG 1 cut(s) 109
SmoI CTYRAG 1 cut(s) 109
SseBI AGGCCT 1 cut(s) 149
SspDI GGCGCC 1 cut(s) 34
SstI GAGCTC 1 cut(s) 17
StuI AGGCCT 1 cut(s) 149
StyD4I CCNGG 1 cut(s) 144
StyI CCWWGG 1 cut(s) 137
TaqI TCGA 3 cut(s) 7, 11, 110
TfiI GAWTC 1 cut(s) 73
Tru1I TTAA 1 cut(s) 220
Tru9I TTAA 1 cut(s) 220
TspDTI ATGAA 1 cut(s) 193
XceI RCATGY 1 cut(s) 104
XhoI CTCGAG 1 cut(s) 109
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.