pycom04g14200

Monothiol glutaredoxin-S16

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
N/A
Physical Location & Seq
Reverse (-)
17086068 .. 17086370
303 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 303 bp
ATGATCAATCCCGTCGCCTCCCTCCATCCGCTTATTCTCTTCTCACTCTTCCCAAAATACCCTAATCCTTCATTCAATTTACCACCAAAAGCCCTCTCCTTTCCCTCCATTTCTCTCAAACCCTACACCGCAACCAAGGCTCGAGCTTTGGTCATCACTTCGCCGGTTAAGACTCTGTCGGGGGCCGATCTAATCCCCGCGCCGGAGGCGGCGGAGACGGGGATCTACGCCCTTTTTTTTTATCAGAACGGCGAGCTGCAGTTCGTCGGGTTATCGAGGAACATAGCGGCGAGTGTTCTCTGA

Protein Analysis

101

Amino Acids

10.75

Weight (kDa)

9.46

Isoelectric Point (pI)

43.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 200
AciI CCGC 6 cut(s) 29, 129, 198, 209, 212, 287
AclWI GGATC 1 cut(s) 230
AfiI CCNNNNNNNGG 1 cut(s) 202
AgsI TTSAA 1 cut(s) 76
AluBI AGCT 2 cut(s) 146, 256
AluI AGCT 2 cut(s) 146, 256
Alw26I GTCTC 1 cut(s) 209
AlwI GGATC 1 cut(s) 230
Ama87I CYCGRG 1 cut(s) 141
AoxI GGCC 1 cut(s) 183
ApeKI GCWGC 1 cut(s) 256
AspLEI GCGC 1 cut(s) 202
AspS9I GGNCC 1 cut(s) 183
AvaI CYCGRG 1 cut(s) 141
BbvI GCAGC 1 cut(s) 243
BccI CCATC 1 cut(s) 33
BceAI ACGGC 1 cut(s) 265
BclI TGATCA 1 cut(s) 3
BcoDI GTCTC 1 cut(s) 209
BfmI CTRYAG 1 cut(s) 257
BisI GCNGC 3 cut(s) 210, 257, 288
BlsI GCNGC 3 cut(s) 211, 258, 289
BmeT110I CYCGRG 1 cut(s) 141
BmgT120I GGNCC 1 cut(s) 183
BmiI GGNNCC 1 cut(s) 184
BsaJI CCNNGG 1 cut(s) 135
Bsc4I CCNNNNNNNGG 1 cut(s) 202
Bse118I RCCGGY 1 cut(s) 163
BseDI CCNNGG 1 cut(s) 135
BseGI GGATG 1 cut(s) 25
BseLI CCNNNNNNNGG 1 cut(s) 202
BseXI GCAGC 1 cut(s) 243
Bsh1236I CGCG 1 cut(s) 200
BshFI GGCC 1 cut(s) 185
BsiHKCI CYCGRG 1 cut(s) 141
BsiSI CCGG 2 cut(s) 164, 203
BslI CCNNNNNNNGG 1 cut(s) 202
BsmAI GTCTC 1 cut(s) 209
BsmBI CGTCTC 1 cut(s) 209
BsnI GGCC 1 cut(s) 185
BsoBI CYCGRG 1 cut(s) 141
Bsp143I GATC 3 cut(s) 3, 187, 222
BspACI CCGC 6 cut(s) 29, 129, 198, 209, 212, 287
BspANI GGCC 1 cut(s) 185
BspFNI CGCG 1 cut(s) 200
BspLI GGNNCC 1 cut(s) 184
BspMAI CTGCAG 1 cut(s) 261
BspPI GGATC 1 cut(s) 230
BsrFI RCCGGY 1 cut(s) 163
BssAI RCCGGY 1 cut(s) 163
BssECI CCNNGG 1 cut(s) 135
BssMI GATC 3 cut(s) 3, 187, 222
BssT1I CCWWGG 1 cut(s) 135
Bst6I CTCTTC 2 cut(s) 44, 53
BstC8I GCNNGC 1 cut(s) 254
BstF5I GGATG 1 cut(s) 25
BstFNI CGCG 1 cut(s) 200
BstHHI GCGC 1 cut(s) 202
BstKTI GATC 3 cut(s) 6, 190, 225
BstMAI GTCTC 1 cut(s) 209
BstMBI GATC 3 cut(s) 3, 187, 222
BstMWI GCNNNNNNNGC 2 cut(s) 137, 206
BstSFI CTRYAG 1 cut(s) 257
BstUI CGCG 1 cut(s) 200
BstV1I GCAGC 1 cut(s) 243
BstX2I RGATCY 1 cut(s) 222
BstYI RGATCY 1 cut(s) 222
BsuRI GGCC 1 cut(s) 185
BtsCI GGATG 1 cut(s) 25
Cac8I GCNNGC 1 cut(s) 254
CfoI GCGC 1 cut(s) 202
Cfr10I RCCGGY 1 cut(s) 163
Cfr13I GGNCC 1 cut(s) 183
CviJI RGCY 5 cut(s) 92, 140, 146, 185, 256
CviKI_1 RGCY 5 cut(s) 92, 140, 146, 185, 256
DpnI GATC 3 cut(s) 5, 189, 224
DpnII GATC 3 cut(s) 3, 187, 222
Eam1104I CTCTTC 2 cut(s) 44, 53
EarI CTCTTC 2 cut(s) 44, 53
EciI GGCGGA 1 cut(s) 227
Eco130I CCWWGG 1 cut(s) 135
Eco88I CYCGRG 1 cut(s) 141
EcoT14I CCWWGG 1 cut(s) 135
ErhI CCWWGG 1 cut(s) 135
Esp3I CGTCTC 1 cut(s) 209
FaiI YATR 1 cut(s) 284
FauI CCCGC 1 cut(s) 205
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 3 cut(s) 210, 257, 288
FokI GGATG 1 cut(s) 12
Fsp4HI GCNGC 3 cut(s) 210, 257, 288
GlaI GCGC 1 cut(s) 201
GluI GCNGC 3 cut(s) 210, 257, 288
HaeIII GGCC 1 cut(s) 185
HapII CCGG 2 cut(s) 164, 203
HhaI GCGC 1 cut(s) 202
Hin6I GCGC 1 cut(s) 200
HinP1I GCGC 1 cut(s) 200
HinfI GANTC 1 cut(s) 172
HpaII CCGG 2 cut(s) 164, 203
Hpy188I TCNGA 2 cut(s) 246, 302
Hpy99I CGWCG 2 cut(s) 17, 269
HpyAV CCTTC 1 cut(s) 78
HpyCH4V TGCA 1 cut(s) 259
HpyF10VI GCNNNNNNNGC 2 cut(s) 137, 206
HspAI GCGC 1 cut(s) 200
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 3 cut(s) 3, 187, 222
LpnPI CCDG 2 cut(s) 177, 216
Lsp1109I GCAGC 1 cut(s) 243
MalI GATC 3 cut(s) 5, 189, 224
MboI GATC 3 cut(s) 3, 187, 222
MboII GAAGA 2 cut(s) 31, 40
MflI RGATCY 1 cut(s) 222
MluCI AATT 1 cut(s) 76
MlyI GAGTC 1 cut(s) 166
MnlI CCTC 6 cut(s) 28, 32, 104, 115, 199, 270
MseI TTAA 1 cut(s) 168
MspI CCGG 2 cut(s) 164, 203
MvnI CGCG 1 cut(s) 200
MwoI GCNNNNNNNGC 2 cut(s) 137, 206
NdeII GATC 3 cut(s) 3, 187, 222
NlaIV GGNNCC 1 cut(s) 184
PaeR7I CTCGAG 1 cut(s) 141
PflFI GACNNNGTC 1 cut(s) 175
PkrI GCNGC 3 cut(s) 211, 258, 289
PleI GAGTC 1 cut(s) 166
PpsI GAGTC 1 cut(s) 166
PspN4I GGNNCC 1 cut(s) 184
PspPI GGNCC 1 cut(s) 183
PspXI VCTCGAGB 1 cut(s) 141
PstI CTGCAG 1 cut(s) 261
PsuI RGATCY 1 cut(s) 222
PsyI GACNNNGTC 1 cut(s) 175
SaqAI TTAA 1 cut(s) 168
SatI GCNGC 3 cut(s) 210, 257, 288
Sau3AI GATC 3 cut(s) 3, 187, 222
Sau96I GGNCC 1 cut(s) 183
SchI GAGTC 1 cut(s) 166
SetI ASST 2 cut(s) 148, 258
SfcI CTRYAG 1 cut(s) 257
Sfr274I CTCGAG 1 cut(s) 141
SlaI CTCGAG 1 cut(s) 141
SmlI CTYRAG 1 cut(s) 141
SmoI CTYRAG 1 cut(s) 141
Sse9I AATT 1 cut(s) 76
SsiI CCGC 6 cut(s) 29, 129, 198, 209, 212, 287
StyI CCWWGG 1 cut(s) 135
TaqI TCGA 2 cut(s) 142, 275
TasI AATT 1 cut(s) 76
TauI GCSGC 2 cut(s) 212, 290
Tru1I TTAA 1 cut(s) 168
Tru9I TTAA 1 cut(s) 168
TseI GCWGC 1 cut(s) 256
TspDTI ATGAA 1 cut(s) 60
Tth111I GACNNNGTC 1 cut(s) 175
XhoI CTCGAG 1 cut(s) 141
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.