pycom04g14570

Ethylene-overproduction protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
N/A
Physical Location & Seq
Forward (+)
17447615 .. 17448436
822 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 468 bp
ATGATGTTTCATGGGAAAATGCATGGTGACCATCTGGTAGAGCTGCTCCACCCTCTTGTTCAACAGTGGAGTCAGGCTGATTGTTGGATGCAACTGTATGATCGATGGTCCTCTGTTGATGATATTGGTTCTCTAGCTGTTGTACATCATATGTTGGCAAATGACCCTGGGAAGAGCCTTCTACACTTTAGGCAGTCTCTCCTTCTGTTGCGGTTAAATTGTCAAAAGGCTGCTATGCATAGTCTTCGGTTGGCTCGGAATCATTCTAGTTCTGAGTATGAGCGGCTTGTCTATGAAGGATGGATCTTCAATGACACTGGCCATCGTGAAGAAGCATTGGCAAAGGCAGAGGAGTCCATTGCTATCCAGAGATCATTTGAAGCATTTTTCCTTAAAGCATATGCTTTAGCAGACTCAAGTCTTGATTCTGAGTCTTCAACATACGTGGATGATCGATCCAGCTTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
BTB TPR_8

Protein Analysis

156

Amino Acids

17.84

Weight (kDa)

5.74

Isoelectric Point (pI)

52.5

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 1 cut(s) 283
AciI CCGC 2 cut(s) 211, 283
AclWI GGATC 2 cut(s) 311, 450
AcoI YGGCCR 1 cut(s) 319
AfaI GTAC 1 cut(s) 144
AgsI TTSAA 4 cut(s) 62, 310, 380, 438
AjnI CCWGG 1 cut(s) 166
AluBI AGCT 3 cut(s) 43, 137, 462
AluI AGCT 3 cut(s) 43, 137, 462
Alw26I GTCTC 1 cut(s) 201
AlwI GGATC 2 cut(s) 311, 450
AoxI GGCC 1 cut(s) 319
ApeKI GCWGC 2 cut(s) 43, 230
AspS9I GGNCC 1 cut(s) 108
AsuHPI GGTGA 1 cut(s) 38
AvaII GGWCC 1 cut(s) 108
BalI TGGCCA 1 cut(s) 321
BbsI GAAGAC 2 cut(s) 236, 426
BbvI GCAGC 2 cut(s) 30, 217
BccI CCATC 4 cut(s) 39, 99, 294, 330
BcgI CGANNNNNNTGC 2 cut(s) 227, 261
BciT130I CCWGG 1 cut(s) 168
BcoDI GTCTC 1 cut(s) 201
BfaI CTAG 3 cut(s) 134, 267, 466
BisI GCNGC 3 cut(s) 44, 231, 284
BlsI GCNGC 3 cut(s) 45, 232, 285
Bme1390I CCNGG 1 cut(s) 168
Bme18I GGWCC 1 cut(s) 108
BmgT120I GGNCC 1 cut(s) 108
BmrFI CCNGG 1 cut(s) 168
BmsI GCATC 1 cut(s) 78
BoxI GACNNNNGTC 1 cut(s) 417
BpiI GAAGAC 2 cut(s) 236, 426
BpuEI CTTGAG 1 cut(s) 400
Bsa29I ATCGAT 2 cut(s) 103, 454
BsaAI YACGTR 1 cut(s) 445
BsaJI CCNNGG 2 cut(s) 166, 167
Bse1I ACTGG 1 cut(s) 322
Bse3DI GCAATG 1 cut(s) 357
BseBI CCWGG 1 cut(s) 168
BseCI ATCGAT 2 cut(s) 103, 454
BseDI CCNNGG 2 cut(s) 166, 167
BseGI GGATG 3 cut(s) 93, 305, 454
BseMI GCAATG 1 cut(s) 357
BseMII CTCAG 2 cut(s) 264, 420
BseNI ACTGG 1 cut(s) 322
BseRI GAGGAG 1 cut(s) 365
BseXI GCAGC 2 cut(s) 30, 217
BshFI GGCC 1 cut(s) 321
BshVI ATCGAT 2 cut(s) 103, 454
BsmAI GTCTC 1 cut(s) 201
BsnI GGCC 1 cut(s) 321
Bsp1407I TGTACA 1 cut(s) 142
Bsp143I GATC 5 cut(s) 100, 303, 371, 451, 455
BspACI CCGC 2 cut(s) 211, 283
BspANI GGCC 1 cut(s) 321
BspCNI CTCAG 2 cut(s) 265, 421
BspDI ATCGAT 2 cut(s) 103, 454
BspPI GGATC 2 cut(s) 311, 450
BspQI GCTCTTC 1 cut(s) 167
BsrBI CCGCTC 1 cut(s) 283
BsrDI GCAATG 1 cut(s) 357
BsrGI TGTACA 1 cut(s) 142
BsrI ACTGG 1 cut(s) 322
BssECI CCNNGG 2 cut(s) 166, 167
BssMI GATC 5 cut(s) 100, 303, 371, 451, 455
Bst2UI CCWGG 1 cut(s) 168
Bst4CI ACNGT 2 cut(s) 66, 96
Bst6I CTCTTC 1 cut(s) 167
BstAUI TGTACA 1 cut(s) 142
BstBAI YACGTR 1 cut(s) 445
BstDEI CTNAG 2 cut(s) 273, 429
BstEII GGTNACC 1 cut(s) 26
BstF5I GGATG 3 cut(s) 93, 305, 454
BstKTI GATC 5 cut(s) 103, 306, 374, 454, 458
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 5 cut(s) 100, 303, 371, 451, 455
BstNI CCWGG 1 cut(s) 168
BstPAI GACNNNNGTC 1 cut(s) 417
BstPI GGTNACC 1 cut(s) 26
BstSCI CCNGG 1 cut(s) 166
BstV1I GCAGC 2 cut(s) 30, 217
BstV2I GAAGAC 2 cut(s) 236, 426
BstX2I RGATCY 1 cut(s) 303
BstYI RGATCY 1 cut(s) 303
Bsu15I ATCGAT 2 cut(s) 103, 454
BsuRI GGCC 1 cut(s) 321
BsuTUI ATCGAT 2 cut(s) 103, 454
BtsCI GGATG 3 cut(s) 93, 305, 454
BtsIMutI CAGTG 2 cut(s) 71, 315
Cfr13I GGNCC 1 cut(s) 108
ClaI ATCGAT 2 cut(s) 103, 454
Csp6I GTAC 1 cut(s) 143
CspCI CAANNNNNGTGG 2 cut(s) 426, 461
CviAII CATG 2 cut(s) 11, 23
CviJI RGCY 9 cut(s) 43, 77, 137, 177, 230, 254, 286, 321, 462
CviKI_1 RGCY 9 cut(s) 43, 77, 137, 177, 230, 254, 286, 321, 462
CviQI GTAC 1 cut(s) 143
DdeI CTNAG 2 cut(s) 273, 429
DpnI GATC 5 cut(s) 102, 305, 373, 453, 457
DpnII GATC 5 cut(s) 100, 303, 371, 451, 455
EaeI YGGCCR 1 cut(s) 319
Eam1104I CTCTTC 1 cut(s) 167
EarI CTCTTC 1 cut(s) 167
Eco47I GGWCC 1 cut(s) 108
Eco91I GGTNACC 1 cut(s) 26
EcoO65I GGTNACC 1 cut(s) 26
EcoRII CCWGG 1 cut(s) 166
EcoT22I ATGCAT 2 cut(s) 24, 240
FaeI CATG 2 cut(s) 14, 26
FatI CATG 2 cut(s) 10, 22
FauNDI CATATG 2 cut(s) 150, 400
Fnu4HI GCNGC 3 cut(s) 44, 231, 284
FokI GGATG 3 cut(s) 100, 312, 461
Fsp4HI GCNGC 3 cut(s) 44, 231, 284
FspBI CTAG 3 cut(s) 134, 267, 466
GluI GCNGC 3 cut(s) 44, 231, 284
HaeIII GGCC 1 cut(s) 321
Hin1II CATG 2 cut(s) 14, 26
HinfI GANTC 6 cut(s) 70, 259, 353, 413, 425, 431
HphI GGTGA 1 cut(s) 38
Hpy188I TCNGA 3 cut(s) 258, 274, 430
Hpy188III TCNNGA 3 cut(s) 326, 367, 422
HpyAV CCTTC 3 cut(s) 188, 212, 290
HpyCH4III ACNGT 2 cut(s) 66, 96
HpyCH4IV ACGT 1 cut(s) 444
HpyCH4V TGCA 3 cut(s) 22, 91, 238
HpyF3I CTNAG 2 cut(s) 273, 429
HpySE526I ACGT 1 cut(s) 444
Hsp92II CATG 2 cut(s) 14, 26
Kzo9I GATC 5 cut(s) 100, 303, 371, 451, 455
LguI GCTCTTC 1 cut(s) 167
LmnI GCTCC 1 cut(s) 51
LpnPI CCDG 6 cut(s) 20, 59, 153, 180, 303, 380
Lsp1109I GCAGC 2 cut(s) 30, 217
LweI GCATC 1 cut(s) 78
MaeI CTAG 3 cut(s) 134, 267, 466
MaeII ACGT 1 cut(s) 444
MaeIII GTNAC 1 cut(s) 26
MalI GATC 5 cut(s) 102, 305, 373, 453, 457
MbiI CCGCTC 1 cut(s) 283
MboI GATC 5 cut(s) 100, 303, 371, 451, 455
MboII GAAGA 5 cut(s) 184, 236, 298, 341, 426
MflI RGATCY 1 cut(s) 303
MlsI TGGCCA 1 cut(s) 321
MluCI AATT 1 cut(s) 217
MluNI TGGCCA 1 cut(s) 321
MlyI GAGTC 4 cut(s) 79, 362, 407, 440
MmeI TCCRAC 1 cut(s) 65
MnlI CCTC 3 cut(s) 63, 121, 343
Mox20I TGGCCA 1 cut(s) 321
Mph1103I ATGCAT 2 cut(s) 24, 240
MscI TGGCCA 1 cut(s) 321
MseI TTAA 2 cut(s) 215, 393
Msp20I TGGCCA 1 cut(s) 321
MspR9I CCNGG 1 cut(s) 168
MvaI CCWGG 1 cut(s) 168
NdeI CATATG 2 cut(s) 150, 400
NdeII GATC 5 cut(s) 100, 303, 371, 451, 455
NlaIII CATG 2 cut(s) 14, 26
NmuCI GTSAC 1 cut(s) 26
NsiI ATGCAT 2 cut(s) 24, 240
PasI CCCWGGG 1 cut(s) 167
PciSI GCTCTTC 1 cut(s) 167
PfeI GAWTC 2 cut(s) 259, 425
PkrI GCNGC 3 cut(s) 45, 232, 285
PleI GAGTC 4 cut(s) 78, 361, 407, 439
PpsI GAGTC 4 cut(s) 78, 361, 407, 439
Ppu21I YACGTR 1 cut(s) 445
PshAI GACNNNNGTC 1 cut(s) 417
Psp6I CCWGG 1 cut(s) 166
PspEI GGTNACC 1 cut(s) 26
PspGI CCWGG 1 cut(s) 166
PspPI GGNCC 1 cut(s) 108
PsuI RGATCY 1 cut(s) 303
RsaI GTAC 1 cut(s) 144
RsaNI GTAC 1 cut(s) 143
SapI GCTCTTC 1 cut(s) 167
SaqAI TTAA 2 cut(s) 215, 393
SatI GCNGC 3 cut(s) 44, 231, 284
Sau3AI GATC 5 cut(s) 100, 303, 371, 451, 455
Sau96I GGNCC 1 cut(s) 108
SchI GAGTC 4 cut(s) 79, 362, 407, 440
ScrFI CCNGG 1 cut(s) 168
SetI ASST 4 cut(s) 45, 139, 447, 464
SfaNI GCATC 1 cut(s) 78
SinI GGWCC 1 cut(s) 108
SmlI CTYRAG 1 cut(s) 415
SmoI CTYRAG 1 cut(s) 415
Sse9I AATT 1 cut(s) 217
SsiI CCGC 2 cut(s) 211, 283
SspMI CTAG 3 cut(s) 134, 267, 466
StyD4I CCNGG 1 cut(s) 166
TaaI ACNGT 2 cut(s) 66, 96
TaiI ACGT 1 cut(s) 447
TaqI TCGA 2 cut(s) 103, 454
TasI AATT 1 cut(s) 217
TatI WGTACW 1 cut(s) 142
TauI GCSGC 1 cut(s) 286
TfiI GAWTC 2 cut(s) 259, 425
Tru1I TTAA 2 cut(s) 215, 393
Tru9I TTAA 2 cut(s) 215, 393
TscAI CASTG 2 cut(s) 71, 322
TseFI GTSAC 1 cut(s) 26
TseI GCWGC 2 cut(s) 43, 230
Tsp45I GTSAC 1 cut(s) 26
TspDTI ATGAA 1 cut(s) 309
TspRI CASTG 2 cut(s) 71, 322
VpaK11BI GGWCC 1 cut(s) 108
XspI CTAG 3 cut(s) 134, 267, 466
Zsp2I ATGCAT 2 cut(s) 24, 240
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.