pycom04g18750
ERF Family

Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
N/A
Physical Location & Seq
Reverse (-)
20658138 .. 20658847
710 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 534 bp
ATGTTTGCAGGTTTCTGCTGCCGGAATGTGCTACAGTTTGTTTCTTGTGGGCTTGGCATTTTTGTATTCGGGTTTCTCCCCAGCAAAACTTTGATACTTCTCTTGTTTGATTTGCTGTTTGCTGTGAAAATAGACGGTCGTGCGTTGAAGTACATTTTTTCGTTATGGCCGGACCTTGGCCTGTGGAAGAAACTTACTCCAGTTGCTCATCGTCATGCTGGTACAATATGGCGGATCACATCTTCCATTCCCTTTCGTTATGGTTATGGTTCAACATACTTGATCGCAATGTCAGGACTATCATGGGTTATTATGTCAGAGTTTGGTGTACCGGTCTTGCCCTTGGGTAATAACATACACCTCCAATTTTATGACGGATTCGAGCTCGGCATCCTTCCTCTCTTCCCTGCCCTTTCGGTATTCTTAAACGTTCAAACGATTTCAGGAACGTTTTTATTCTACTCAAAGCTGGTGCCGGAGACCAAGGAGAGAACCCTAGAAGAAATACAAGCATCAATTGCCCATTTCGTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Sugar_tr

Protein Analysis

178

Amino Acids

19.92

Weight (kDa)

8.83

Isoelectric Point (pI)

44.91

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 472
AciI CCGC 1 cut(s) 232
AclI AACGTT 2 cut(s) 429, 449
AclWI GGATC 1 cut(s) 242
AcoI YGGCCR 1 cut(s) 167
AfaI GTAC 3 cut(s) 152, 223, 330
AfiI CCNNNNNNNGG 1 cut(s) 176
AgeI ACCGGT 1 cut(s) 331
AgsI TTSAA 3 cut(s) 148, 273, 434
AluBI AGCT 2 cut(s) 385, 469
AluI AGCT 2 cut(s) 385, 469
Alw21I GWGCWC 1 cut(s) 387
Alw26I GTCTC 1 cut(s) 473
AlwI GGATC 1 cut(s) 242
AoxI GGCC 2 cut(s) 167, 178
ApeKI GCWGC 1 cut(s) 18
AsiGI ACCGGT 1 cut(s) 331
AspS9I GGNCC 1 cut(s) 172
AvaII GGWCC 1 cut(s) 172
BanI GGYRCC 1 cut(s) 472
BanII GRGCYC 1 cut(s) 387
Bbv12I GWGCWC 1 cut(s) 387
BbvI GCAGC 1 cut(s) 5
BcoDI GTCTC 1 cut(s) 473
BfaI CTAG 1 cut(s) 497
BfmI CTRYAG 1 cut(s) 32
BisI GCNGC 1 cut(s) 19
BlsI GCNGC 1 cut(s) 20
Bme18I GGWCC 1 cut(s) 172
BmgT120I GGNCC 1 cut(s) 172
BmiI GGNNCC 1 cut(s) 474
BmsI GCATC 2 cut(s) 399, 521
BpmI CTGGAG 1 cut(s) 183
BsaI GGTCTC 1 cut(s) 473
BsaJI CCNNGG 3 cut(s) 175, 342, 483
BsaWI WCCGGW 1 cut(s) 331
Bsc4I CCNNNNNNNGG 1 cut(s) 176
Bse118I RCCGGY 1 cut(s) 331
Bse1I ACTGG 1 cut(s) 200
Bse3DI GCAATG 1 cut(s) 294
BseDI CCNNGG 3 cut(s) 175, 342, 483
BseGI GGATG 1 cut(s) 390
BseLI CCNNNNNNNGG 1 cut(s) 176
BseMI GCAATG 1 cut(s) 294
BseNI ACTGG 1 cut(s) 200
BseXI GCAGC 1 cut(s) 5
BseYI CCCAGC 1 cut(s) 80
Bsh1285I CGRYCG 1 cut(s) 139
BshFI GGCC 2 cut(s) 169, 180
BshNI GGYRCC 1 cut(s) 472
BshTI ACCGGT 1 cut(s) 331
BsiEI CGRYCG 1 cut(s) 139
BsiHKAI GWGCWC 1 cut(s) 387
BsiSI CCGG 4 cut(s) 22, 170, 332, 476
BslI CCNNNNNNNGG 1 cut(s) 176
BsmAI GTCTC 1 cut(s) 473
BsnI GGCC 2 cut(s) 169, 180
Bso31I GGTCTC 1 cut(s) 473
Bsp1286I GDGCHC 1 cut(s) 387
Bsp143I GATC 2 cut(s) 234, 282
BspACI CCGC 1 cut(s) 232
BspANI GGCC 2 cut(s) 169, 180
BspLI GGNNCC 1 cut(s) 474
BspPI GGATC 1 cut(s) 242
BspT107I GGYRCC 1 cut(s) 472
BspTNI GGTCTC 1 cut(s) 473
BsrDI GCAATG 1 cut(s) 294
BsrFI RCCGGY 1 cut(s) 331
BsrI ACTGG 1 cut(s) 200
BssAI RCCGGY 1 cut(s) 331
BssECI CCNNGG 3 cut(s) 175, 342, 483
BssMI GATC 2 cut(s) 234, 282
BssT1I CCWWGG 3 cut(s) 175, 342, 483
Bst4CI ACNGT 2 cut(s) 36, 137
Bst6I CTCTTC 1 cut(s) 407
BstAPI GCANNNNNTGC 1 cut(s) 518
BstF5I GGATG 1 cut(s) 390
BstKTI GATC 2 cut(s) 237, 285
BstMAI GTCTC 1 cut(s) 473
BstMBI GATC 2 cut(s) 234, 282
BstMCI CGRYCG 1 cut(s) 139
BstMWI GCNNNNNNNGC 1 cut(s) 518
BstSFI CTRYAG 1 cut(s) 32
BstV1I GCAGC 1 cut(s) 5
BsuRI GGCC 2 cut(s) 169, 180
BtsCI GGATG 1 cut(s) 390
Cfr10I RCCGGY 1 cut(s) 331
Cfr13I GGNCC 1 cut(s) 172
Csp6I GTAC 3 cut(s) 151, 222, 329
CspAI ACCGGT 1 cut(s) 331
CviAII CATG 2 cut(s) 215, 303
CviJI RGCY 5 cut(s) 52, 169, 180, 385, 469
CviKI_1 RGCY 5 cut(s) 52, 169, 180, 385, 469
CviQI GTAC 3 cut(s) 151, 222, 329
DpnI GATC 2 cut(s) 236, 284
DpnII GATC 2 cut(s) 234, 282
EaeI YGGCCR 1 cut(s) 167
Eam1104I CTCTTC 1 cut(s) 407
EarI CTCTTC 1 cut(s) 407
EciI GGCGGA 1 cut(s) 247
Ecl136II GAGCTC 1 cut(s) 385
Eco130I CCWWGG 3 cut(s) 175, 342, 483
Eco24I GRGCYC 1 cut(s) 387
Eco31I GGTCTC 1 cut(s) 473
Eco47I GGWCC 1 cut(s) 172
Eco53kI GAGCTC 1 cut(s) 385
EcoICRI GAGCTC 1 cut(s) 385
EcoT14I CCWWGG 3 cut(s) 175, 342, 483
EcoT38I GRGCYC 1 cut(s) 387
ErhI CCWWGG 3 cut(s) 175, 342, 483
FaeI CATG 2 cut(s) 218, 306
FatI CATG 2 cut(s) 214, 302
Fnu4HI GCNGC 1 cut(s) 19
FokI GGATG 1 cut(s) 377
FriOI GRGCYC 1 cut(s) 387
Fsp4HI GCNGC 1 cut(s) 19
FspBI CTAG 1 cut(s) 497
GluI GCNGC 1 cut(s) 19
GsaI CCCAGC 1 cut(s) 84
GsuI CTGGAG 1 cut(s) 183
HaeIII GGCC 2 cut(s) 169, 180
HapII CCGG 4 cut(s) 22, 170, 332, 476
Hin1II CATG 2 cut(s) 218, 306
HinfI GANTC 1 cut(s) 378
HpaII CCGG 4 cut(s) 22, 170, 332, 476
Hpy166II GTNNAC 1 cut(s) 329
Hpy188I TCNGA 1 cut(s) 319
Hpy188III TCNNGA 2 cut(s) 294, 444
Hpy8I GTNNAC 1 cut(s) 329
HpyAV CCTTC 1 cut(s) 404
HpyCH4III ACNGT 2 cut(s) 36, 137
HpyCH4IV ACGT 2 cut(s) 429, 449
HpyCH4V TGCA 1 cut(s) 8
HpyF10VI GCNNNNNNNGC 1 cut(s) 518
HpySE526I ACGT 2 cut(s) 429, 449
Hsp92II CATG 2 cut(s) 218, 306
Kzo9I GATC 2 cut(s) 234, 282
Lsp1109I GCAGC 1 cut(s) 5
LweI GCATC 2 cut(s) 399, 521
MaeI CTAG 1 cut(s) 497
MaeII ACGT 2 cut(s) 429, 449
MalI GATC 2 cut(s) 236, 284
MboI GATC 2 cut(s) 234, 282
MboII GAAGA 4 cut(s) 199, 234, 394, 512
MfeI CAATTG 1 cut(s) 516
MhlI GDGCHC 1 cut(s) 387
MluCI AATT 2 cut(s) 365, 516
MnlI CCTC 2 cut(s) 371, 408
MseI TTAA 2 cut(s) 425, 532
MslI CAYNNNNRTG 1 cut(s) 213
MspI CCGG 4 cut(s) 22, 170, 332, 476
MunI CAATTG 1 cut(s) 516
MwoI GCNNNNNNNGC 1 cut(s) 518
NdeII GATC 2 cut(s) 234, 282
NlaIII CATG 2 cut(s) 218, 306
NlaIV GGNNCC 1 cut(s) 474
NmeAIII GCCGAG 1 cut(s) 366
PfeI GAWTC 1 cut(s) 378
PinAI ACCGGT 1 cut(s) 331
PkrI GCNGC 1 cut(s) 20
Psp124BI GAGCTC 1 cut(s) 387
Psp1406I AACGTT 2 cut(s) 429, 449
PspFI CCCAGC 1 cut(s) 80
PspN4I GGNNCC 1 cut(s) 474
PspPI GGNCC 1 cut(s) 172
RsaI GTAC 3 cut(s) 152, 223, 330
RsaNI GTAC 3 cut(s) 151, 222, 329
RseI CAYNNNNRTG 1 cut(s) 213
SacI GAGCTC 1 cut(s) 387
SaqAI TTAA 2 cut(s) 425, 532
SatI GCNGC 1 cut(s) 19
Sau3AI GATC 2 cut(s) 234, 282
Sau96I GGNCC 1 cut(s) 172
SduI GDGCHC 1 cut(s) 387
SetI ASST 7 cut(s) 13, 177, 363, 387, 432, 452, 471
SfaNI GCATC 2 cut(s) 399, 521
SfcI CTRYAG 1 cut(s) 32
SinI GGWCC 1 cut(s) 172
SmiMI CAYNNNNRTG 1 cut(s) 213
Sse9I AATT 2 cut(s) 365, 516
SsiI CCGC 1 cut(s) 232
SspMI CTAG 1 cut(s) 497
SstI GAGCTC 1 cut(s) 387
StyI CCWWGG 3 cut(s) 175, 342, 483
TaaI ACNGT 2 cut(s) 36, 137
TaiI ACGT 2 cut(s) 432, 452
TaqI TCGA 1 cut(s) 381
TasI AATT 2 cut(s) 365, 516
TatI WGTACW 1 cut(s) 150
TfiI GAWTC 1 cut(s) 378
Tru1I TTAA 2 cut(s) 425, 532
Tru9I TTAA 2 cut(s) 425, 532
TseI GCWGC 1 cut(s) 18
TspGWI ACGGA 1 cut(s) 390
VpaK11BI GGWCC 1 cut(s) 172
XspI CTAG 1 cut(s) 497
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.