pycom04g20260

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
N/A
Physical Location & Seq
Forward (+)
21787272 .. 21787809
538 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 435 bp
ATGATAAAACAAAGGGAAAAAAAAGATAACAAGATCACGAAGGAGGAGGAAGAGACCAAGAGATTCCTCAGGTCAAAAAGAGAAGCCTTGGTTCTTTGTTTAGAGATAGCTGCTCGGCATTATAAAACTCCATGGTGCCAAACCGTCATAGACATATCAGCCAAGCGCGCTTTTGATAACGTAGCAAGGTTCACATGGAAACAAAGGAGGGCCATTGAGAAACTTCGGGCTTCCATACGGGAACAGCATAATCGTACAAAACAATGGAAATCGGTCACTGGGAAACTCGATGCCAATGCTTTCGAGTGGTTGCAGCAGAAGTATGCTACTGAGAAGATCAGTATTCGACATTCAGTTGGAAATAGTGGGATCGTAAAACCGAAATGTGGCTTGGATGACAACATATTATCGGTATGGATGTTGATCATTCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

145

Amino Acids

17.0

Weight (kDa)

10.01

Isoelectric Point (pI)

59.28

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 123
AccB1I GGYRCC 1 cut(s) 135
AccII CGCG 1 cut(s) 168
AclWI GGATC 1 cut(s) 377
AfaI GTAC 1 cut(s) 256
AfiI CCNNNNNNNGG 1 cut(s) 386
AjuI GAANNNNNNNTTGG 2 cut(s) 374, 406
AluBI AGCT 1 cut(s) 110
AluI AGCT 1 cut(s) 110
Alw26I GTCTC 1 cut(s) 47
AlwI GGATC 1 cut(s) 377
AoxI GGCC 1 cut(s) 210
ApeKI GCWGC 2 cut(s) 110, 313
AspLEI GCGC 2 cut(s) 168, 170
AspS9I GGNCC 1 cut(s) 210
AxyI CCTNAGG 1 cut(s) 68
BanI GGYRCC 1 cut(s) 135
BbvI GCAGC 2 cut(s) 97, 325
BcgI CGANNNNNNTGC 2 cut(s) 278, 312
BclI TGATCA 1 cut(s) 423
BcoDI GTCTC 1 cut(s) 47
BisI GCNGC 2 cut(s) 111, 314
BlsI GCNGC 2 cut(s) 112, 315
BmgT120I GGNCC 1 cut(s) 210
BmiI GGNNCC 1 cut(s) 137
BmrI ACTGGG 1 cut(s) 288
BmsI GCATC 1 cut(s) 280
BmuI ACTGGG 1 cut(s) 288
BsaBI GATNNNNATC 1 cut(s) 422
BsaI GGTCTC 1 cut(s) 47
BsaJI CCNNGG 2 cut(s) 87, 131
Bsc4I CCNNNNNNNGG 1 cut(s) 386
Bse1I ACTGG 1 cut(s) 283
Bse21I CCTNAGG 1 cut(s) 68
Bse8I GATNNNNATC 1 cut(s) 422
BseDI CCNNGG 2 cut(s) 87, 131
BseGI GGATG 2 cut(s) 400, 423
BseJI GATNNNNATC 1 cut(s) 422
BseLI CCNNNNNNNGG 1 cut(s) 386
BseMII CTCAG 2 cut(s) 82, 321
BseNI ACTGG 1 cut(s) 283
BsePI GCGCGC 1 cut(s) 166
BseRI GAGGAG 1 cut(s) 59
BseXI GCAGC 2 cut(s) 97, 325
Bsh1236I CGCG 1 cut(s) 168
BshFI GGCC 1 cut(s) 212
BshNI GGYRCC 1 cut(s) 135
BslI CCNNNNNNNGG 1 cut(s) 386
BsmAI GTCTC 1 cut(s) 47
BsnI GGCC 1 cut(s) 212
Bso31I GGTCTC 1 cut(s) 47
Bsp143I GATC 4 cut(s) 33, 336, 369, 423
Bsp19I CCATGG 1 cut(s) 131
BspANI GGCC 1 cut(s) 212
BspCNI CTCAG 2 cut(s) 81, 322
BspFNI CGCG 1 cut(s) 168
BspLI GGNNCC 1 cut(s) 137
BspPI GGATC 1 cut(s) 377
BspT107I GGYRCC 1 cut(s) 135
BspTNI GGTCTC 1 cut(s) 47
BsrI ACTGG 1 cut(s) 283
BssECI CCNNGG 2 cut(s) 87, 131
BssHII GCGCGC 1 cut(s) 166
BssMI GATC 4 cut(s) 33, 336, 369, 423
BssT1I CCWWGG 2 cut(s) 87, 131
Bst4CI ACNGT 1 cut(s) 145
Bst6I CTCTTC 1 cut(s) 45
BstC8I GCNNGC 1 cut(s) 168
BstDEI CTNAG 2 cut(s) 68, 330
BstDSI CCRYGG 1 cut(s) 131
BstF5I GGATG 2 cut(s) 400, 423
BstFNI CGCG 1 cut(s) 168
BstHHI GCGC 2 cut(s) 168, 170
BstKTI GATC 4 cut(s) 36, 339, 372, 426
BstMAI GTCTC 1 cut(s) 47
BstMBI GATC 4 cut(s) 33, 336, 369, 423
BstMWI GCNNNNNNNGC 1 cut(s) 167
BstUI CGCG 1 cut(s) 168
BstV1I GCAGC 2 cut(s) 97, 325
Bsu36I CCTNAGG 1 cut(s) 68
BsuRI GGCC 1 cut(s) 212
BtgI CCRYGG 1 cut(s) 131
BtsCI GGATG 2 cut(s) 400, 423
BtsIMutI CAGTG 1 cut(s) 276
Cac8I GCNNGC 1 cut(s) 168
CfoI GCGC 2 cut(s) 168, 170
Cfr13I GGNCC 1 cut(s) 210
Csp6I GTAC 1 cut(s) 255
CviAII CATG 2 cut(s) 132, 195
CviJI RGCY 6 cut(s) 86, 110, 161, 212, 230, 390
CviKI_1 RGCY 6 cut(s) 86, 110, 161, 212, 230, 390
CviQI GTAC 1 cut(s) 255
DdeI CTNAG 2 cut(s) 68, 330
DpnI GATC 4 cut(s) 35, 338, 371, 425
DpnII GATC 4 cut(s) 33, 336, 369, 423
Eam1104I CTCTTC 1 cut(s) 45
EarI CTCTTC 1 cut(s) 45
Eco130I CCWWGG 2 cut(s) 87, 131
Eco31I GGTCTC 1 cut(s) 47
Eco81I CCTNAGG 1 cut(s) 68
EcoT14I CCWWGG 2 cut(s) 87, 131
ErhI CCWWGG 2 cut(s) 87, 131
FaeI CATG 2 cut(s) 135, 198
FatI CATG 2 cut(s) 131, 194
FbaI TGATCA 1 cut(s) 423
Fnu4HI GCNGC 2 cut(s) 111, 314
FokI GGATG 2 cut(s) 407, 430
Fsp4HI GCNGC 2 cut(s) 111, 314
GlaI GCGC 2 cut(s) 167, 169
GluI GCNGC 2 cut(s) 111, 314
HaeIII GGCC 1 cut(s) 212
HhaI GCGC 2 cut(s) 168, 170
Hin1II CATG 2 cut(s) 135, 198
Hin6I GCGC 2 cut(s) 166, 168
HinP1I GCGC 2 cut(s) 166, 168
HinfI GANTC 1 cut(s) 63
Hpy166II GTNNAC 1 cut(s) 192
Hpy188III TCNNGA 1 cut(s) 37
Hpy8I GTNNAC 1 cut(s) 192
HpyAV CCTTC 1 cut(s) 34
HpyCH4III ACNGT 1 cut(s) 145
HpyCH4IV ACGT 1 cut(s) 180
HpyCH4V TGCA 1 cut(s) 313
HpyF10VI GCNNNNNNNGC 1 cut(s) 167
HpyF3I CTNAG 2 cut(s) 68, 330
HpySE526I ACGT 1 cut(s) 180
Hsp92II CATG 2 cut(s) 135, 198
HspAI GCGC 2 cut(s) 166, 168
Ksp22I TGATCA 1 cut(s) 423
Kzo9I GATC 4 cut(s) 33, 336, 369, 423
LpnPI CCDG 2 cut(s) 55, 264
Lsp1109I GCAGC 2 cut(s) 97, 325
LweI GCATC 1 cut(s) 280
MaeII ACGT 1 cut(s) 180
MaeIII GTNAC 1 cut(s) 274
MalI GATC 4 cut(s) 35, 338, 371, 425
MboI GATC 4 cut(s) 33, 336, 369, 423
MboII GAAGA 2 cut(s) 62, 346
MmeI TCCRAC 1 cut(s) 337
MnlI CCTC 4 cut(s) 37, 40, 77, 201
MvnI CGCG 1 cut(s) 168
MwoI GCNNNNNNNGC 1 cut(s) 167
NcoI CCATGG 1 cut(s) 131
NdeII GATC 4 cut(s) 33, 336, 369, 423
NlaIII CATG 2 cut(s) 135, 198
NlaIV GGNNCC 1 cut(s) 137
NmeAIII GCCGAG 1 cut(s) 94
NmuCI GTSAC 1 cut(s) 274
PauI GCGCGC 1 cut(s) 166
PfeI GAWTC 1 cut(s) 63
PkrI GCNGC 2 cut(s) 112, 315
PsiI TTATAA 1 cut(s) 123
PspN4I GGNNCC 1 cut(s) 137
PspPI GGNCC 1 cut(s) 210
PteI GCGCGC 1 cut(s) 166
RsaI GTAC 1 cut(s) 256
RsaNI GTAC 1 cut(s) 255
SatI GCNGC 2 cut(s) 111, 314
Sau3AI GATC 4 cut(s) 33, 336, 369, 423
Sau96I GGNCC 1 cut(s) 210
SetI ASST 4 cut(s) 74, 112, 183, 191
SfaNI GCATC 1 cut(s) 280
StyI CCWWGG 2 cut(s) 87, 131
TaaI ACNGT 1 cut(s) 145
TaiI ACGT 1 cut(s) 183
TaqI TCGA 3 cut(s) 288, 303, 346
TaqII GACCGA 1 cut(s) 262
TfiI GAWTC 1 cut(s) 63
TscAI CASTG 1 cut(s) 283
TseFI GTSAC 1 cut(s) 274
TseI GCWGC 2 cut(s) 110, 313
Tsp45I GTSAC 1 cut(s) 274
TspRI CASTG 1 cut(s) 283
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.