pycom04g22000

Introduction of a cis double bond between carbons of the acyl chain

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
N/A
Physical Location & Seq
Forward (+)
22950967 .. 22951506
540 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 540 bp
ATGTTAGACTTGGAAAGGCCATGGCCAGTCCTTCAAATCCTCGTCAGCTGCTTGTTGCACCTTGGAACTCCTCATACCAGCAGCGCCACCACCACCACTTCCCTCAACGGAGGCATAGTACCTCAGGAAGAAGGCGAGTGTGAGCACCACCCACTCCAAGCAGAATATCACCACGCTCAGCCCTCCTGCCAATTTCAGAATCACACTCCCATCCTCTTCCCTCACGTACGACTTGAGCTCCCCGAGAAAGTCCCCCGTCCTCGTGAACACCAGCACCGCCACCGACCCTTGGAATATCGCCGTCAGCACCGTCGACACCATGTGCGCTCCGTACCATTTGGAGCTCGACCTGCTCCCCGCCGCCGCACATCCCGATATGGCCCCGGCAATTGTCAGCACGTGGAGCAGGATCGAGAAGAAGCCGCACACCGACGGCAGCAGCCGCAGAGACAGCGTCAGGAAGATGCAGCTGGAGGCTGCTCCCAGCAATATGTAGTTGCTCAGCAGGAACACCTTGTGGGTGTGGTATTCCGACTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0016491 GO:0016705 GO:0016717 GO:0045300 GO:0055114
KEGG Pathways
Metabolic & Signaling
ko00061 ko01040 ko01212 map00061 map01040 map01212
Pfam Domains
Protein Families
FA_desaturase_2

Protein Analysis

180

Amino Acids

20.81

Weight (kDa)

9.25

Isoelectric Point (pI)

73.93

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 358
AccI GTMKAC 1 cut(s) 313
AciI CCGC 6 cut(s) 277, 358, 361, 364, 423, 443
AclWI GGATC 1 cut(s) 417
AcoI YGGCCR 1 cut(s) 23
AcvI CACGTG 1 cut(s) 400
AdeI CACNNNGTG 2 cut(s) 322, 517
AfaI GTAC 3 cut(s) 120, 228, 333
AfiI CCNNNNNNNGG 1 cut(s) 289
AgsI TTSAA 1 cut(s) 35
AluBI AGCT 4 cut(s) 48, 238, 344, 470
AluI AGCT 4 cut(s) 48, 238, 344, 470
Alw21I GWGCWC 3 cut(s) 147, 240, 346
Alw26I GTCTC 1 cut(s) 442
AlwI GGATC 1 cut(s) 417
Ama87I CYCGRG 1 cut(s) 242
AoxI GGCC 3 cut(s) 17, 23, 379
ApeKI GCWGC 6 cut(s) 48, 81, 436, 439, 467, 477
AspLEI GCGC 2 cut(s) 86, 327
AspS9I GGNCC 1 cut(s) 380
AsuC2I CCSGG 1 cut(s) 384
AsuHPI GGTGA 1 cut(s) 161
AvaI CYCGRG 1 cut(s) 242
AxyI CCTNAGG 1 cut(s) 123
BalI TGGCCA 1 cut(s) 25
BanII GRGCYC 2 cut(s) 240, 346
BauI CACGAG 1 cut(s) 261
BbrPI CACGTG 1 cut(s) 400
Bbv12I GWGCWC 3 cut(s) 147, 240, 346
BbvI GCAGC 6 cut(s) 35, 93, 448, 451, 464, 479
BccI CCATC 1 cut(s) 218
BceAI ACGGC 2 cut(s) 285, 449
BcnI CCSGG 1 cut(s) 384
BcoDI GTCTC 1 cut(s) 442
BfoI RGCGCY 1 cut(s) 87
BfuAI ACCTGC 1 cut(s) 358
BlpI GCTNAGC 2 cut(s) 177, 501
Bme1390I CCNGG 1 cut(s) 384
BmeT110I CYCGRG 1 cut(s) 242
BmgT120I GGNCC 1 cut(s) 380
BmiI GGNNCC 1 cut(s) 382
BmrFI CCNGG 1 cut(s) 384
BmsI GCATC 1 cut(s) 454
BpmI CTGGAG 1 cut(s) 492
Bpu1102I GCTNAGC 2 cut(s) 177, 501
BpuEI CTTGAG 1 cut(s) 254
BpuMI CCSGG 1 cut(s) 384
BsaAI YACGTR 2 cut(s) 226, 400
BsaJI CCNNGG 4 cut(s) 20, 61, 288, 382
BsaXI ACNNNNNCTCC 4 cut(s) 102, 132, 222, 252
Bsc4I CCNNNNNNNGG 1 cut(s) 289
Bse1I ACTGG 1 cut(s) 26
Bse21I CCTNAGG 1 cut(s) 123
BseDI CCNNGG 4 cut(s) 20, 61, 288, 382
BseGI GGATG 2 cut(s) 210, 368
BseLI CCNNNNNNNGG 1 cut(s) 289
BseMII CTCAG 3 cut(s) 137, 191, 515
BseNI ACTGG 1 cut(s) 26
BseRI GAGGAG 1 cut(s) 60
BseXI GCAGC 6 cut(s) 35, 93, 448, 451, 464, 479
BseYI CCCAGC 1 cut(s) 483
BshFI GGCC 3 cut(s) 19, 25, 381
BsiHKAI GWGCWC 3 cut(s) 147, 240, 346
BsiHKCI CYCGRG 1 cut(s) 242
BsiSI CCGG 1 cut(s) 384
BsiWI CGTACG 1 cut(s) 226
BslFI GGGAC 1 cut(s) 236
BslI CCNNNNNNNGG 1 cut(s) 289
BsmAI GTCTC 1 cut(s) 442
BsmFI GGGAC 1 cut(s) 236
BsnI GGCC 3 cut(s) 19, 25, 381
BsoBI CYCGRG 1 cut(s) 242
Bsp1286I GDGCHC 3 cut(s) 147, 240, 346
Bsp143I GATC 1 cut(s) 409
Bsp1720I GCTNAGC 2 cut(s) 177, 501
Bsp19I CCATGG 1 cut(s) 20
BspACI CCGC 6 cut(s) 277, 358, 361, 364, 423, 443
BspANI GGCC 3 cut(s) 19, 25, 381
BspCNI CTCAG 3 cut(s) 136, 190, 514
BspLI GGNNCC 1 cut(s) 382
BspMI ACCTGC 1 cut(s) 358
BspPI GGATC 1 cut(s) 417
BsrI ACTGG 1 cut(s) 26
BssECI CCNNGG 4 cut(s) 20, 61, 288, 382
BssMI GATC 1 cut(s) 409
BssSI CACGAG 1 cut(s) 261
BssT1I CCWWGG 3 cut(s) 20, 61, 288
Bst2BI CACGAG 1 cut(s) 261
Bst4CI ACNGT 1 cut(s) 311
Bst6I CTCTTC 1 cut(s) 221
BstBAI YACGTR 2 cut(s) 226, 400
BstDEI CTNAG 3 cut(s) 123, 177, 501
BstDSI CCRYGG 1 cut(s) 20
BstF5I GGATG 2 cut(s) 210, 368
BstH2I RGCGCY 1 cut(s) 87
BstHHI GCGC 2 cut(s) 86, 327
BstKTI GATC 1 cut(s) 412
BstMAI GTCTC 1 cut(s) 442
BstMBI GATC 1 cut(s) 409
BstMWI GCNNNNNNNGC 4 cut(s) 350, 403, 442, 451
BstSCI CCNGG 1 cut(s) 382
BstV1I GCAGC 6 cut(s) 35, 93, 448, 451, 464, 479
Bsu36I CCTNAGG 1 cut(s) 123
BsuRI GGCC 3 cut(s) 19, 25, 381
BtgI CCRYGG 1 cut(s) 20
BtsCI GGATG 2 cut(s) 210, 368
BveI ACCTGC 1 cut(s) 358
CfoI GCGC 2 cut(s) 86, 327
Cfr13I GGNCC 1 cut(s) 380
CseI GACGC 1 cut(s) 443
Csp6I GTAC 3 cut(s) 119, 227, 332
CviAII CATG 2 cut(s) 21, 320
CviQI GTAC 3 cut(s) 119, 227, 332
DdeI CTNAG 3 cut(s) 123, 177, 501
DpnI GATC 1 cut(s) 411
DpnII GATC 1 cut(s) 409
DraIII CACNNNGTG 2 cut(s) 322, 517
EaeI YGGCCR 1 cut(s) 23
Eam1104I CTCTTC 1 cut(s) 221
EarI CTCTTC 1 cut(s) 221
Ecl136II GAGCTC 2 cut(s) 238, 344
Eco130I CCWWGG 3 cut(s) 20, 61, 288
Eco24I GRGCYC 2 cut(s) 240, 346
Eco53kI GAGCTC 2 cut(s) 238, 344
Eco72I CACGTG 1 cut(s) 400
Eco81I CCTNAGG 1 cut(s) 123
Eco88I CYCGRG 1 cut(s) 242
EcoICRI GAGCTC 2 cut(s) 238, 344
EcoT14I CCWWGG 3 cut(s) 20, 61, 288
EcoT38I GRGCYC 2 cut(s) 240, 346
ErhI CCWWGG 3 cut(s) 20, 61, 288
FaeI CATG 2 cut(s) 24, 323
FaiI YATR 6 cut(s) 22, 75, 116, 321, 378, 492
FaqI GGGAC 1 cut(s) 236
FatI CATG 2 cut(s) 20, 319
FauI CCCGC 1 cut(s) 365
FblI GTMKAC 1 cut(s) 313
FokI GGATG 2 cut(s) 197, 355
FriOI GRGCYC 2 cut(s) 240, 346
GlaI GCGC 2 cut(s) 85, 326
GsaI CCCAGC 1 cut(s) 487
GsuI CTGGAG 1 cut(s) 492
HaeII RGCGCY 1 cut(s) 87
HaeIII GGCC 3 cut(s) 19, 25, 381
HapII CCGG 1 cut(s) 384
HgaI GACGC 1 cut(s) 443
HhaI GCGC 2 cut(s) 86, 327
Hin1II CATG 2 cut(s) 24, 323
Hin6I GCGC 2 cut(s) 84, 325
HinP1I GCGC 2 cut(s) 84, 325
HincII GTYRAC 1 cut(s) 314
HindII GTYRAC 1 cut(s) 314
HinfI GANTC 2 cut(s) 199, 534
HpaII CCGG 1 cut(s) 384
HphI GGTGA 1 cut(s) 161
Hpy166II GTNNAC 2 cut(s) 266, 314
Hpy188I TCNGA 3 cut(s) 198, 533, 539
Hpy188III TCNNGA 5 cut(s) 125, 263, 372, 413, 458
Hpy8I GTNNAC 2 cut(s) 266, 314
Hpy99I CGWCG 2 cut(s) 315, 435
HpyAV CCTTC 2 cut(s) 41, 125
HpyCH4III ACNGT 1 cut(s) 311
HpyCH4IV ACGT 2 cut(s) 225, 399
HpyCH4V TGCA 2 cut(s) 58, 467
HpyF10VI GCNNNNNNNGC 4 cut(s) 350, 403, 442, 451
HpyF3I CTNAG 3 cut(s) 123, 177, 501
HpySE526I ACGT 2 cut(s) 225, 399
Hsp92II CATG 2 cut(s) 24, 323
HspAI GCGC 2 cut(s) 84, 325
Kzo9I GATC 1 cut(s) 409
LmnI GCTCC 6 cut(s) 243, 332, 341, 358, 403, 485
Lsp1109I GCAGC 6 cut(s) 35, 93, 448, 451, 464, 479
LweI GCATC 1 cut(s) 454
MaeII ACGT 2 cut(s) 225, 399
MalI GATC 1 cut(s) 411
MboI GATC 1 cut(s) 409
MboII GAAGA 4 cut(s) 140, 208, 428, 473
MfeI CAATTG 1 cut(s) 388
MhlI GDGCHC 3 cut(s) 147, 240, 346
MlsI TGGCCA 1 cut(s) 25
MluCI AATT 2 cut(s) 191, 388
MluNI TGGCCA 1 cut(s) 25
MlyI GAGTC 1 cut(s) 528
Mox20I TGGCCA 1 cut(s) 25
MscI TGGCCA 1 cut(s) 25
Msp20I TGGCCA 1 cut(s) 25
MspA1I CMGCKG 2 cut(s) 48, 470
MspI CCGG 1 cut(s) 384
MspR9I CCNGG 1 cut(s) 384
MunI CAATTG 1 cut(s) 388
MwoI GCNNNNNNNGC 4 cut(s) 350, 403, 442, 451
NciI CCSGG 1 cut(s) 384
NcoI CCATGG 1 cut(s) 20
NdeII GATC 1 cut(s) 409
NlaIII CATG 2 cut(s) 24, 323
NlaIV GGNNCC 1 cut(s) 382
PfeI GAWTC 1 cut(s) 199
Pfl23II CGTACG 1 cut(s) 226
PflFI GACNNNGTC 1 cut(s) 453
PleI GAGTC 1 cut(s) 528
PmaCI CACGTG 1 cut(s) 400
PmlI CACGTG 1 cut(s) 400
PpsI GAGTC 1 cut(s) 528
Ppu21I YACGTR 2 cut(s) 226, 400
Psp124BI GAGCTC 2 cut(s) 240, 346
PspCI CACGTG 1 cut(s) 400
PspFI CCCAGC 1 cut(s) 483
PspLI CGTACG 1 cut(s) 226
PspN4I GGNNCC 1 cut(s) 382
PspPI GGNCC 1 cut(s) 380
PsrI GAACNNNNNNTAC 2 cut(s) 58, 90
PsyI GACNNNGTC 1 cut(s) 453
PvuII CAGCTG 2 cut(s) 48, 470
RsaI GTAC 3 cut(s) 120, 228, 333
RsaNI GTAC 3 cut(s) 119, 227, 332
SacI GAGCTC 2 cut(s) 240, 346
SalI GTCGAC 1 cut(s) 312
Sau3AI GATC 1 cut(s) 409
Sau96I GGNCC 1 cut(s) 380
SchI GAGTC 1 cut(s) 528
ScrFI CCNGG 1 cut(s) 384
SduI GDGCHC 3 cut(s) 147, 240, 346
SfaNI GCATC 1 cut(s) 454
SmlI CTYRAG 1 cut(s) 233
SmoI CTYRAG 1 cut(s) 233
Sse9I AATT 2 cut(s) 191, 388
SsiI CCGC 6 cut(s) 277, 358, 361, 364, 423, 443
SstI GAGCTC 2 cut(s) 240, 346
StyD4I CCNGG 1 cut(s) 382
StyI CCWWGG 3 cut(s) 20, 61, 288
TaaI ACNGT 1 cut(s) 311
TaiI ACGT 2 cut(s) 228, 402
TaqI TCGA 3 cut(s) 313, 346, 412
TasI AATT 2 cut(s) 191, 388
TauI GCSGC 4 cut(s) 363, 366, 425, 445
TfiI GAWTC 1 cut(s) 199
TseI GCWGC 6 cut(s) 48, 81, 436, 439, 467, 477
TspGWI ACGGA 2 cut(s) 123, 319
Tth111I GACNNNGTC 1 cut(s) 453
XmiI GTMKAC 1 cut(s) 313
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.