pycom05g00730

psbP domain-containing protein 4

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
N/A
Physical Location & Seq
Reverse (-)
935335 .. 936534
1200 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 477 bp
ATGAGAACAGCCTCACTTACCGGTTGGAGCTTCTCCTGGACTCATCAGCGGCAAGCAATTCCTTCCCACAATTTGGTTGTTCAGTCTTCATTGGAGGATGGAAATTCCAGGACAAAAGTTAGCTCAAGTGCGACAGAGAGTGGCTTGGTTGATGGAGAAGCTGAGAATTTCTCCGGAATTTTGAAGAGACGATCCGTGTTGCTTTCTGCAGGAGCTTCTTTGTTTTCTTCTCAAGTGTTGGGTTTTCCGGGAGAGGGATTGGCAGTGGTGAAACAAGGTCTTCTAGCCGGCCGGATTCCCGGTCTGTCCGAACCGGATGAACAAGGTTGGAGGGCATACCGAAGACCAGATGAGAAGTCCGGAGGGCATGGCGTTGGTTGGAGCCCTATCATCCCTTATCGCTTTTCAGTTCCTCAAGATTGGGAAGAGGTTCCAGTATCAATAGCGGATCGAGGCGGTACAGATCGATCGATTTGA

Protein Analysis

159

Amino Acids

17.01

Weight (kDa)

6.29

Isoelectric Point (pI)

50.58

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 73
AccIII TCCGGA 2 cut(s) 173, 359
AciI CCGC 3 cut(s) 49, 446, 456
AclWI GGATC 2 cut(s) 186, 456
AcoI YGGCCR 1 cut(s) 289
AcsI RAATTY 3 cut(s) 103, 166, 177
AfaI GTAC 1 cut(s) 460
AfiI CCNNNNNNNGG 2 cut(s) 73, 254
AgeI ACCGGT 1 cut(s) 20
AgsI TTSAA 1 cut(s) 184
AjnI CCWGG 2 cut(s) 35, 107
AluBI AGCT 4 cut(s) 30, 123, 161, 215
AluI AGCT 4 cut(s) 30, 123, 161, 215
Alw26I GTCTC 1 cut(s) 181
AlwI GGATC 2 cut(s) 186, 456
Aor13HI TCCGGA 2 cut(s) 173, 359
AoxI GGCC 1 cut(s) 289
ApoI RAATTY 3 cut(s) 103, 166, 177
AsiGI ACCGGT 1 cut(s) 20
Asp700I GAANNNNTTC 1 cut(s) 429
AsuC2I CCSGG 2 cut(s) 249, 300
AsuHPI GGTGA 1 cut(s) 280
BanII GRGCYC 1 cut(s) 386
BbsI GAAGAC 3 cut(s) 78, 272, 349
BccI CCATC 2 cut(s) 92, 146
BciT130I CCWGG 2 cut(s) 37, 109
BcnI CCSGG 2 cut(s) 249, 300
BcoDI GTCTC 1 cut(s) 181
BfaI CTAG 1 cut(s) 284
BfmI CTRYAG 1 cut(s) 207
BisI GCNGC 1 cut(s) 50
BlsI GCNGC 1 cut(s) 51
Bme1390I CCNGG 4 cut(s) 37, 109, 249, 300
BmiI GGNNCC 2 cut(s) 383, 432
BmrFI CCNGG 4 cut(s) 37, 109, 249, 300
BpiI GAAGAC 3 cut(s) 78, 272, 349
BplI GAGNNNNNCTC 2 cut(s) 155, 187
BpuEI CTTGAG 3 cut(s) 109, 216, 399
BpuMI CCSGG 2 cut(s) 249, 300
Bsa29I ATCGAT 2 cut(s) 466, 470
BsaWI WCCGGW 4 cut(s) 20, 173, 313, 359
Bsc4I CCNNNNNNNGG 2 cut(s) 73, 254
Bse118I RCCGGY 2 cut(s) 20, 287
Bse1I ACTGG 1 cut(s) 434
BseAI TCCGGA 2 cut(s) 173, 359
BseBI CCWGG 2 cut(s) 37, 109
BseCI ATCGAT 2 cut(s) 466, 470
BseGI GGATG 3 cut(s) 103, 322, 390
BseLI CCNNNNNNNGG 2 cut(s) 73, 254
BseMII CTCAG 1 cut(s) 153
BseNI ACTGG 1 cut(s) 434
BseX3I CGGCCG 1 cut(s) 289
Bsh1285I CGRYCG 2 cut(s) 292, 470
BshFI GGCC 1 cut(s) 291
BshTI ACCGGT 1 cut(s) 20
BshVI ATCGAT 2 cut(s) 466, 470
BsiEI CGRYCG 2 cut(s) 292, 470
BsiSI CCGG 8 cut(s) 21, 174, 248, 288, 292, 300, 314, 360
BslI CCNNNNNNNGG 2 cut(s) 73, 254
BsmAI GTCTC 1 cut(s) 181
BsmBI CGTCTC 1 cut(s) 181
BsnI GGCC 1 cut(s) 291
Bsp1286I GDGCHC 1 cut(s) 386
Bsp13I TCCGGA 2 cut(s) 173, 359
Bsp143I GATC 4 cut(s) 191, 448, 463, 467
BspACI CCGC 3 cut(s) 49, 446, 456
BspANI GGCC 1 cut(s) 291
BspCNI CTCAG 1 cut(s) 154
BspDI ATCGAT 2 cut(s) 466, 470
BspEI TCCGGA 2 cut(s) 173, 359
BspLI GGNNCC 2 cut(s) 383, 432
BspMAI CTGCAG 1 cut(s) 211
BspPI GGATC 2 cut(s) 186, 456
BsrFI RCCGGY 2 cut(s) 20, 287
BsrI ACTGG 1 cut(s) 434
BssAI RCCGGY 2 cut(s) 20, 287
BssMI GATC 4 cut(s) 191, 448, 463, 467
Bst2UI CCWGG 2 cut(s) 37, 109
Bst6I CTCTTC 2 cut(s) 179, 420
BstC8I GCNNGC 2 cut(s) 54, 289
BstDEI CTNAG 1 cut(s) 162
BstF5I GGATG 3 cut(s) 103, 322, 390
BstKTI GATC 4 cut(s) 194, 451, 466, 470
BstMAI GTCTC 1 cut(s) 181
BstMBI GATC 4 cut(s) 191, 448, 463, 467
BstMCI CGRYCG 2 cut(s) 292, 470
BstNI CCWGG 2 cut(s) 37, 109
BstSCI CCNGG 4 cut(s) 35, 107, 247, 298
BstSFI CTRYAG 1 cut(s) 207
BstV2I GAAGAC 3 cut(s) 78, 272, 349
BstZI CGGCCG 1 cut(s) 289
Bsu15I ATCGAT 2 cut(s) 466, 470
BsuRI GGCC 1 cut(s) 291
BsuTUI ATCGAT 2 cut(s) 466, 470
BtsCI GGATG 3 cut(s) 103, 322, 390
BtsI GCAGTG 1 cut(s) 270
BtsIMutI CAGTG 1 cut(s) 270
Cac8I GCNNGC 2 cut(s) 54, 289
Cfr10I RCCGGY 2 cut(s) 20, 287
ClaI ATCGAT 2 cut(s) 466, 470
Csp6I GTAC 1 cut(s) 459
CspAI ACCGGT 1 cut(s) 20
CviAII CATG 1 cut(s) 368
CviJI RGCY 9 cut(s) 11, 30, 123, 144, 161, 215, 287, 291, 384
CviKI_1 RGCY 9 cut(s) 11, 30, 123, 144, 161, 215, 287, 291, 384
CviQI GTAC 1 cut(s) 459
DdeI CTNAG 1 cut(s) 162
DpnI GATC 4 cut(s) 193, 450, 465, 469
DpnII GATC 4 cut(s) 191, 448, 463, 467
EaeI YGGCCR 1 cut(s) 289
EagI CGGCCG 1 cut(s) 289
Eam1104I CTCTTC 2 cut(s) 179, 420
EarI CTCTTC 2 cut(s) 179, 420
EclXI CGGCCG 1 cut(s) 289
Eco24I GRGCYC 1 cut(s) 386
Eco52I CGGCCG 1 cut(s) 289
EcoRII CCWGG 2 cut(s) 35, 107
EcoT38I GRGCYC 1 cut(s) 386
Esp3I CGTCTC 1 cut(s) 181
FaeI CATG 1 cut(s) 371
FaiI YATR 2 cut(s) 337, 369
FatI CATG 1 cut(s) 367
Fnu4HI GCNGC 1 cut(s) 50
FokI GGATG 3 cut(s) 110, 329, 377
FriOI GRGCYC 1 cut(s) 386
Fsp4HI GCNGC 1 cut(s) 50
FspBI CTAG 1 cut(s) 284
GluI GCNGC 1 cut(s) 50
HaeIII GGCC 1 cut(s) 291
HapII CCGG 8 cut(s) 21, 174, 248, 288, 292, 300, 314, 360
Hin1II CATG 1 cut(s) 371
HinfI GANTC 2 cut(s) 40, 295
HpaII CCGG 8 cut(s) 21, 174, 248, 288, 292, 300, 314, 360
HphI GGTGA 1 cut(s) 280
Hpy188I TCNGA 1 cut(s) 310
Hpy188III TCNNGA 3 cut(s) 174, 360, 416
HpyAV CCTTC 1 cut(s) 72
HpyCH4V TGCA 1 cut(s) 209
HpyF3I CTNAG 1 cut(s) 162
Hsp92II CATG 1 cut(s) 371
Kpn2I TCCGGA 2 cut(s) 173, 359
KroI GCCGGC 1 cut(s) 287
KroNI GCCGGC 1 cut(s) 289
Kzo9I GATC 4 cut(s) 191, 448, 463, 467
LmnI GCTCC 3 cut(s) 27, 212, 381
MaeI CTAG 1 cut(s) 284
MalI GATC 4 cut(s) 193, 450, 465, 469
MboI GATC 4 cut(s) 191, 448, 463, 467
MboII GAAGA 6 cut(s) 78, 196, 219, 272, 354, 437
MhlI GDGCHC 1 cut(s) 386
MluCI AATT 5 cut(s) 57, 70, 103, 166, 177
MlyI GAGTC 1 cut(s) 34
MmeI TCCRAC 3 cut(s) 5, 308, 359
MnlI CCTC 8 cut(s) 22, 88, 247, 324, 356, 421, 423, 446
MroI TCCGGA 2 cut(s) 173, 359
MroNI GCCGGC 1 cut(s) 287
MroXI GAANNNNTTC 1 cut(s) 429
MspA1I CMGCKG 1 cut(s) 49
MspI CCGG 8 cut(s) 21, 174, 248, 288, 292, 300, 314, 360
MspR9I CCNGG 4 cut(s) 37, 109, 249, 300
MvaI CCWGG 2 cut(s) 37, 109
NaeI GCCGGC 1 cut(s) 289
NciI CCSGG 2 cut(s) 249, 300
NdeII GATC 4 cut(s) 191, 448, 463, 467
NgoMIV GCCGGC 1 cut(s) 287
NlaIII CATG 1 cut(s) 371
NlaIV GGNNCC 2 cut(s) 383, 432
PdiI GCCGGC 1 cut(s) 289
PdmI GAANNNNTTC 1 cut(s) 429
PfeI GAWTC 1 cut(s) 295
PflMI CCANNNNNTGG 1 cut(s) 73
PfoI TCCNGGA 3 cut(s) 35, 107, 247
PinAI ACCGGT 1 cut(s) 20
PkrI GCNGC 1 cut(s) 51
Ple19I CGATCG 1 cut(s) 470
PleI GAGTC 1 cut(s) 34
PpsI GAGTC 1 cut(s) 34
Psp6I CCWGG 2 cut(s) 35, 107
PspGI CCWGG 2 cut(s) 35, 107
PspN4I GGNNCC 2 cut(s) 383, 432
PstI CTGCAG 1 cut(s) 211
PvuI CGATCG 1 cut(s) 470
RsaI GTAC 1 cut(s) 460
RsaNI GTAC 1 cut(s) 459
SatI GCNGC 1 cut(s) 50
Sau3AI GATC 4 cut(s) 191, 448, 463, 467
SchI GAGTC 1 cut(s) 34
ScrFI CCNGG 4 cut(s) 37, 109, 249, 300
SduI GDGCHC 1 cut(s) 386
SetI ASST 7 cut(s) 32, 125, 163, 217, 280, 328, 432
SfcI CTRYAG 1 cut(s) 207
SmlI CTYRAG 3 cut(s) 124, 231, 414
SmoI CTYRAG 3 cut(s) 124, 231, 414
Sse9I AATT 5 cut(s) 57, 70, 103, 166, 177
SsiI CCGC 3 cut(s) 49, 446, 456
SspMI CTAG 1 cut(s) 284
StyD4I CCNGG 4 cut(s) 35, 107, 247, 298
TaqI TCGA 3 cut(s) 451, 466, 470
TasI AATT 5 cut(s) 57, 70, 103, 166, 177
TauI GCSGC 1 cut(s) 52
TfiI GAWTC 1 cut(s) 295
TscAI CASTG 1 cut(s) 270
TspDTI ATGAA 2 cut(s) 78, 333
TspGWI ACGGA 1 cut(s) 184
TspRI CASTG 1 cut(s) 270
Van91I CCANNNNNTGG 1 cut(s) 73
XapI RAATTY 3 cut(s) 103, 166, 177
XmnI GAANNNNTTC 1 cut(s) 429
XspI CTAG 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.