pycom05g02540

Large subunit GTPase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
N/A
Physical Location & Seq
Forward (+)
3032231 .. 3032932
702 bp
Loading structure...
UTR
Exon/CDS
Intron
N/A

Sequence Viewer

Length: 702 bp
ATGTCTGACGAGTTAATGCTATGTGATTGCCCTGGATTGGTCTTTCCATCCTTTTCAAGCTCAAGACATGAAATGATTGCTTCTGGTGTATTGCCAATTGATCGAATGACTGAGAATAGGGAGGCTGTGCAGGTAGTGGCCAATCGAATTCCACGGCATGTTATTGAAGCGGTTTATAGGATTGATCTGCCCAAGCCCAAATCCTATGAACTGCAGTCTCGACCACCTTTGGCAGCAGAATTTCTGAGGGTCTATTGTGCCTCTCGTGGTTATGTCGCCTCAAGTGGGCTTCCTGATGAAACGAGAGCTGCCCGCCAAATTTTGAAGGATTACATTGATGGAAAGCTGCCCCATTATCAAATGCCCCCTGGAATGACTGAGGAAGATGCTGTGGGAAACTGCTTATCTGAGCAGCATAAATCAGACTCCTCTGAGAATGAACACTCTGTAGATGATGAAGGTGAAGACGAGCCAGAGCTTGATCATGTCCTGGAAGATCTCAATTCATTTGACTTAGCTAATGGGCTGGCTACCAAGAAGAAAGTCACTGTCAGAAAGCCCACTGCGGCCCATAAACAGCACAAAAAGACTCAGAGGAAGAAGGATCGCTCCTGGAGGGTAGGGAATGACGGAGGAGATGGAATGCCAGTTGTAAGAGCCTTTCAGAAACCAGCAAACACCGGTCCTCTTAAGGTTGGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0000003 GO:0000054 GO:0000166 GO:0001882 GO:0001883 GO:0003006 GO:0003674 GO:0003824 GO:0003924 GO:0005488 GO:0005525 GO:0005575 GO:0005622 GO:0005623 GO:0005634 GO:0005654 GO:0005730 GO:0005737 GO:0005783 GO:0005829 GO:0006139 GO:0006364 GO:0006396 GO:0006403 GO:0006405 GO:0006611 GO:0006725 GO:0006807 GO:0006810 GO:0006886 GO:0006913 GO:0007275 GO:0008104 GO:0008150 GO:0008152 GO:0009790 GO:0009791 GO:0009793 GO:0009966 GO:0009987 GO:0010154 GO:0010467 GO:0010646 GO:0010817 GO:0012505 GO:0015030 GO:0015031 GO:0015833 GO:0015931 GO:0016070 GO:0016072 GO:0016462 GO:0016604 GO:0016787 GO:0016817 GO:0016818 GO:0017076 GO:0017111 GO:0019001 GO:0022414 GO:0022613 GO:0023051 GO:0031503 GO:0031974 GO:0031981 GO:0032501 GO:0032502 GO:0032549 GO:0032550 GO:0032553 GO:0032555 GO:0032561 GO:0032879 GO:0033036 GO:0033750 GO:0034470 GO:0034613 GO:0034641 GO:0034660 GO:0035639 GO:0036094 GO:0042254 GO:0042886 GO:0043021 GO:0043022 GO:0043167 GO:0043168 GO:0043170 GO:0043226 GO:0043227 GO:0043228 GO:0043229 GO:0043231 GO:0043232 GO:0043233 GO:0044085 GO:0044087 GO:0044089 GO:0044237 GO:0044238 GO:0044422 GO:0044424 GO:0044428 GO:0044444 GO:0044446 GO:0044451 GO:0044464 GO:0044877 GO:0045184 GO:0046483 GO:0046626 GO:0046907 GO:0048316 GO:0048366 GO:0048367 GO:0048518 GO:0048583 GO:0048608 GO:0048731 GO:0048825 GO:0048827 GO:0048856 GO:0050657 GO:0050658 GO:0050789 GO:0050794 GO:0051049 GO:0051168 GO:0051169 GO:0051179 GO:0051234 GO:0051236 GO:0051640 GO:0051641 GO:0051649 GO:0051656 GO:0061458 GO:0065007 GO:0065008 GO:0070013 GO:0070727 GO:0071166 GO:0071426 GO:0071428 GO:0071702 GO:0071704 GO:0071705 GO:0071840 GO:0090069 GO:0090070 GO:0090304 GO:0097159 GO:0097367 GO:0099402 GO:1900076 GO:1901265 GO:1901360 GO:1901363 GO:2000012
KEGG Pathways
Metabolic & Signaling
ko03008 map03008
Pfam Domains
Protein Families
MMR_HSR1

Protein Analysis

234

Amino Acids

25.93

Weight (kDa)

6.08

Isoelectric Point (pI)

48.35

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes

No orthologs found for this gene in the current database.

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 121
AciI CCGC 3 cut(s) 170, 313, 566
AclWI GGATC 1 cut(s) 612
AcoI YGGCCR 1 cut(s) 138
AcsI RAATTY 3 cut(s) 147, 239, 318
AfiI CCNNNNNNNGG 2 cut(s) 37, 285
AflII CTTAAG 1 cut(s) 689
AgeI ACCGGT 1 cut(s) 680
AgsI TTSAA 3 cut(s) 57, 167, 325
AjnI CCWGG 4 cut(s) 31, 367, 489, 611
AluBI AGCT 5 cut(s) 60, 308, 346, 478, 518
AluI AGCT 5 cut(s) 60, 308, 346, 478, 518
Alw26I GTCTC 1 cut(s) 222
AlwI GGATC 1 cut(s) 612
AoxI GGCC 2 cut(s) 138, 567
ApeKI GCWGC 4 cut(s) 233, 308, 346, 412
ApoI RAATTY 3 cut(s) 147, 239, 318
AsiGI ACCGGT 1 cut(s) 680
AspS9I GGNCC 2 cut(s) 568, 683
AsuHPI GGTGA 1 cut(s) 473
AvaII GGWCC 1 cut(s) 683
BalI TGGCCA 1 cut(s) 140
BauI CACGAG 1 cut(s) 264
BbsI GAAGAC 1 cut(s) 471
BbvI GCAGC 4 cut(s) 245, 295, 333, 424
BccI CCATC 3 cut(s) 55, 332, 632
BceAI ACGGC 1 cut(s) 170
BciT130I CCWGG 4 cut(s) 33, 369, 491, 613
BclI TGATCA 1 cut(s) 481
BcoDI GTCTC 1 cut(s) 222
BfmI CTRYAG 2 cut(s) 212, 447
BfrI CTTAAG 1 cut(s) 689
BfuAI ACCTGC 1 cut(s) 121
BglII AGATCT 1 cut(s) 496
BisI GCNGC 5 cut(s) 234, 309, 347, 413, 567
BlsI GCNGC 5 cut(s) 235, 310, 348, 414, 568
Bme1390I CCNGG 4 cut(s) 33, 369, 491, 613
Bme18I GGWCC 1 cut(s) 683
BmgT120I GGNCC 2 cut(s) 568, 683
BmrFI CCNGG 4 cut(s) 33, 369, 491, 613
BmsI GCATC 1 cut(s) 376
BpiI GAAGAC 1 cut(s) 471
BpmI CTGGAG 1 cut(s) 634
BpuEI CTTGAG 2 cut(s) 46, 265
BsaJI CCNNGG 3 cut(s) 31, 152, 367
BsaWI WCCGGW 1 cut(s) 680
Bsc4I CCNNNNNNNGG 2 cut(s) 37, 285
Bse118I RCCGGY 1 cut(s) 680
Bse1I ACTGG 1 cut(s) 647
BseBI CCWGG 4 cut(s) 33, 369, 491, 613
BseDI CCNNGG 3 cut(s) 31, 152, 367
BseGI GGATG 1 cut(s) 47
BseLI CCNNNNNNNGG 2 cut(s) 37, 285
BseMII CTCAG 6 cut(s) 102, 236, 369, 399, 423, 605
BseNI ACTGG 1 cut(s) 647
BseRI GAGGAG 2 cut(s) 418, 648
BseXI GCAGC 4 cut(s) 245, 295, 333, 424
BsgI GTGCAG 1 cut(s) 149
BshFI GGCC 2 cut(s) 140, 569
BshTI ACCGGT 1 cut(s) 680
BsiSI CCGG 1 cut(s) 681
BslI CCNNNNNNNGG 2 cut(s) 37, 285
BsmAI GTCTC 1 cut(s) 222
BsmI GAATGC 1 cut(s) 648
BsnI GGCC 2 cut(s) 140, 569
Bsp143I GATC 5 cut(s) 100, 184, 481, 496, 604
BspACI CCGC 3 cut(s) 170, 313, 566
BspANI GGCC 2 cut(s) 140, 569
BspCNI CTCAG 6 cut(s) 103, 237, 370, 400, 424, 604
BspMAI CTGCAG 1 cut(s) 216
BspMI ACCTGC 1 cut(s) 121
BspPI GGATC 1 cut(s) 612
BspTI CTTAAG 1 cut(s) 689
BsrFI RCCGGY 1 cut(s) 680
BsrI ACTGG 1 cut(s) 647
BssAI RCCGGY 1 cut(s) 680
BssECI CCNNGG 3 cut(s) 31, 152, 367
BssMI GATC 5 cut(s) 100, 184, 481, 496, 604
BssSI CACGAG 1 cut(s) 264
Bst2BI CACGAG 1 cut(s) 264
Bst2UI CCWGG 4 cut(s) 33, 369, 491, 613
Bst4CI ACNGT 1 cut(s) 550
BstAFI CTTAAG 1 cut(s) 689
BstC8I GCNNGC 2 cut(s) 313, 528
BstDEI CTNAG 7 cut(s) 111, 245, 378, 408, 432, 514, 591
BstDSI CCRYGG 1 cut(s) 152
BstF5I GGATG 1 cut(s) 47
BstKTI GATC 5 cut(s) 103, 187, 484, 499, 607
BstMAI GTCTC 1 cut(s) 222
BstMBI GATC 5 cut(s) 100, 184, 481, 496, 604
BstNI CCWGG 4 cut(s) 33, 369, 491, 613
BstNSI RCATGY 1 cut(s) 161
BstSCI CCNGG 4 cut(s) 31, 367, 489, 611
BstSFI CTRYAG 2 cut(s) 212, 447
BstV1I GCAGC 4 cut(s) 245, 295, 333, 424
BstV2I GAAGAC 1 cut(s) 471
BstX2I RGATCY 1 cut(s) 496
BstYI RGATCY 1 cut(s) 496
BsuRI GGCC 2 cut(s) 140, 569
BtgI CCRYGG 1 cut(s) 152
BtsCI GGATG 1 cut(s) 47
BtsI GCAGTG 1 cut(s) 561
BtsIMutI CAGTG 2 cut(s) 546, 561
BveI ACCTGC 1 cut(s) 121
Cac8I GCNNGC 2 cut(s) 313, 528
Cfr10I RCCGGY 1 cut(s) 680
Cfr13I GGNCC 2 cut(s) 568, 683
CspAI ACCGGT 1 cut(s) 680
CviAII CATG 3 cut(s) 68, 158, 485
DdeI CTNAG 7 cut(s) 111, 245, 378, 408, 432, 514, 591
DpnI GATC 5 cut(s) 102, 186, 483, 498, 606
DpnII GATC 5 cut(s) 100, 184, 481, 496, 604
EaeI YGGCCR 1 cut(s) 138
Eco47I GGWCC 1 cut(s) 683
EcoRI GAATTC 1 cut(s) 147
EcoRII CCWGG 4 cut(s) 31, 367, 489, 611
FaeI CATG 3 cut(s) 71, 161, 488
FaiI YATR 9 cut(s) 22, 69, 159, 177, 207, 273, 417, 486, 573
FatI CATG 3 cut(s) 67, 157, 484
FauI CCCGC 1 cut(s) 320
FbaI TGATCA 1 cut(s) 481
Fnu4HI GCNGC 5 cut(s) 234, 309, 347, 413, 567
FokI GGATG 1 cut(s) 34
Fsp4HI GCNGC 5 cut(s) 234, 309, 347, 413, 567
GluI GCNGC 5 cut(s) 234, 309, 347, 413, 567
GsuI CTGGAG 1 cut(s) 634
HaeIII GGCC 2 cut(s) 140, 569
HapII CCGG 1 cut(s) 681
Hin1II CATG 3 cut(s) 71, 161, 488
HinfI GANTC 2 cut(s) 425, 589
HpaII CCGG 1 cut(s) 681
HphI GGTGA 1 cut(s) 473
Hpy188I TCNGA 8 cut(s) 7, 246, 409, 424, 433, 554, 594, 666
Hpy188III TCNNGA 3 cut(s) 63, 219, 293
HpyAV CCTTC 3 cut(s) 319, 452, 595
HpyCH4III ACNGT 1 cut(s) 550
HpyCH4V TGCA 2 cut(s) 130, 214
HpyF3I CTNAG 7 cut(s) 111, 245, 378, 408, 432, 514, 591
Hsp92II CATG 3 cut(s) 71, 161, 488
Ksp22I TGATCA 1 cut(s) 481
Kzo9I GATC 5 cut(s) 100, 184, 481, 496, 604
LmnI GCTCC 1 cut(s) 614
Lsp1109I GCAGC 4 cut(s) 245, 295, 333, 424
LweI GCATC 1 cut(s) 376
MaeIII GTNAC 1 cut(s) 544
MalI GATC 5 cut(s) 102, 186, 483, 498, 606
MboI GATC 5 cut(s) 100, 184, 481, 496, 604
MboII GAAGA 5 cut(s) 395, 476, 506, 550, 610
MfeI CAATTG 1 cut(s) 96
MflI RGATCY 1 cut(s) 496
MlsI TGGCCA 1 cut(s) 140
MluCI AATT 5 cut(s) 96, 147, 239, 318, 502
MluNI TGGCCA 1 cut(s) 140
MlyI GAGTC 2 cut(s) 419, 583
MmeI TCCRAC 1 cut(s) 676
Mox20I TGGCCA 1 cut(s) 140
MscI TGGCCA 1 cut(s) 140
MseI TTAA 2 cut(s) 14, 690
Msp20I TGGCCA 1 cut(s) 140
MspCI CTTAAG 1 cut(s) 689
MspI CCGG 1 cut(s) 681
MspR9I CCNGG 4 cut(s) 33, 369, 491, 613
MunI CAATTG 1 cut(s) 96
Mva1269I GAATGC 1 cut(s) 648
MvaI CCWGG 4 cut(s) 33, 369, 491, 613
NdeII GATC 5 cut(s) 100, 184, 481, 496, 604
NlaIII CATG 3 cut(s) 71, 161, 488
NmuCI GTSAC 1 cut(s) 544
NspI RCATGY 1 cut(s) 161
PctI GAATGC 1 cut(s) 648
PfoI TCCNGGA 2 cut(s) 489, 611
PinAI ACCGGT 1 cut(s) 680
PkrI GCNGC 5 cut(s) 235, 310, 348, 414, 568
PleI GAGTC 2 cut(s) 419, 583
PpsI GAGTC 2 cut(s) 419, 583
Psp6I CCWGG 4 cut(s) 31, 367, 489, 611
PspGI CCWGG 4 cut(s) 31, 367, 489, 611
PspPI GGNCC 2 cut(s) 568, 683
PstI CTGCAG 1 cut(s) 216
PsuI RGATCY 1 cut(s) 496
SaqAI TTAA 2 cut(s) 14, 690
SatI GCNGC 5 cut(s) 234, 309, 347, 413, 567
Sau3AI GATC 5 cut(s) 100, 184, 481, 496, 604
Sau96I GGNCC 2 cut(s) 568, 683
SchI GAGTC 2 cut(s) 419, 583
ScrFI CCNGG 4 cut(s) 33, 369, 491, 613
SetI ASST 9 cut(s) 62, 135, 229, 310, 348, 463, 480, 520, 696
SfaNI GCATC 1 cut(s) 376
SfcI CTRYAG 2 cut(s) 212, 447
SinI GGWCC 1 cut(s) 683
SmlI CTYRAG 3 cut(s) 61, 280, 689
SmoI CTYRAG 3 cut(s) 61, 280, 689
Sse9I AATT 5 cut(s) 96, 147, 239, 318, 502
SsiI CCGC 3 cut(s) 170, 313, 566
StyD4I CCNGG 4 cut(s) 31, 367, 489, 611
TaaI ACNGT 1 cut(s) 550
TaqI TCGA 3 cut(s) 103, 145, 220
TasI AATT 5 cut(s) 96, 147, 239, 318, 502
TauI GCSGC 1 cut(s) 569
Tru1I TTAA 2 cut(s) 14, 690
Tru9I TTAA 2 cut(s) 14, 690
TscAI CASTG 2 cut(s) 553, 568
TseFI GTSAC 1 cut(s) 544
TseI GCWGC 4 cut(s) 233, 308, 346, 412
Tsp45I GTSAC 1 cut(s) 544
TspDTI ATGAA 6 cut(s) 84, 222, 312, 453, 471, 495
TspGWI ACGGA 1 cut(s) 645
TspRI CASTG 2 cut(s) 553, 568
Vha464I CTTAAG 1 cut(s) 689
VpaK11BI GGWCC 1 cut(s) 683
XapI RAATTY 3 cut(s) 147, 239, 318
XceI RCATGY 1 cut(s) 161
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.